Pcdha4 (BC060211) Mouse Tagged ORF Clone

CAT#: MR209688

  • TrueORF®

Pcdha4 (Myc-DDK-tagged) - Mouse protocadherin alpha 4 (cDNA clone MGC:64735 IMAGE:6838078)

ORF Plasmid: DDK tGFP



  "BC060211" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 4,768.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms Cnr1; Crnr1; R75250
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR209688 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAATTTTCCTGGGGAAGTGGCCAGGAATCCCAGCGCTTGCTTCTTTCTTTTCTGCTTCTTGCAATCT
GGGAGGCAGGGAACAGCCAGATCCACTACTCCATCCCTGAGGAGGCCAAACACGGCACCTTCGTGGGCCG
CATCGCGCAGGACCTGGGGCTGGAGCTGACGGAGCTGGTGCCCCGCCTGTTCAGAGTGGCGTCCAAGGAC
CGCGGAGACCTTCTGGAGGTAAATCTGCAGAATGGCATTTTGTTTGTGAATTCTCGGATCGACCGGGAGG
AGCTGTGCGGGCGGAGCGCGGAGTGCAGCATCCACCTGGAGGTGATCGTGGACAGGCCGTTGCAGGTTTT
CCACGTGGAGGTGGAGGTGAGGGACATTAACGACAACCCTCCCAGGTTCCCAACAACACAAAAGAATCTG
TTCATTGCAGAATCAAGGCCACTTGACACTTGGTTTCCACTAGAGGGCGCTTCAGACGCAGATATCGGAA
TCAATGCTGTACTGACTTACAGACTGAGTCCAAATGATTACTTTTCTTTGGAAAAACCATCCAACGACGA
ACGGGTAAAAGGTCTTGGACTTGTATTACGGAAATCTTTAGACCGGGAGGAAACTCCAGAGATAATTTTA
GTGCTTACTGTCACGGACGGAGGAAAGCCAGAGCTGACCGGCAGTGTTCAGTTACTCATCACTGTGCTGG
ATGCCAATGATAATGCTCCAGTTTTTGACAGATCTCTGTATACCGTGAAATTACCAGAAAACGTTCCAAA
TGGGACATTGGTAGTCAAAGTCAATGCCTCAGATTTAGACGAAGGGGTAAATGGGGATATTATGTACTCA
TTTTCTACAGATATTTCACCAAATGTGAAATACAAATTCCACATAGACCCTGTTAGCGGAGAGATTATTG
TAAAGGGATACATTGATTTTGAAGAATGCAAATCCTATGAAATTCTCATAGAGGGAATTGACAAGGGACA
ACTTCCACTCTCTGGGCACTGTAAAGTCATTGTACAAGTTGAAGACATCAACGATAATGTTCCAGAATTG
GAATTCAAATCTCTATCACTTCCAATACGAGAGAATTCTCCAGTGGGCACTGTCATCGCACTCATTAGTG
TGTCTGATCGGGACACGGGTGTCAACGGGCAGGTGACCTGCTCCCTGACAAGTCATGTCCCCTTCAAGTT
GGTGTCCACATTCAAGAATTACTATTCGCTCGTGCTGGACAGCGCCCTGGACAGAGAGACAACAGCGGAC
TATAAGGTGGTGGTGACAGCGCGGGATGGGGGCTCTCCCTCGCTGTGGGCCACGGCTAGCGTGTCTGTTG
AGGTTGCTGACGTGAACGACAATGCACCTGTGTTCGCGCAGCCCGAATACACGGTGTTCGTGAAGGAGAA
CAACCCGCCTGGTGCGCACATCTTCACGGTGTCAGCGATGGATGCGGACGCACAGGAGAACGCGCTGGTG
TCCTACTCGCTGGTGGAGCGGAGGGTGGGCGAGCGCTTGCTGTCGAGCTATGTGTCTGTGCACGCGGAGA
GCGGCAAGGTGTTCGCGCTGCAGCCTCTGGACCATGAGGAGCTGGAGCTGCTGCGGTTCCAGCCCCGGCA
GCCCAACCCTGACTGGCGCTACTCTGCCTCGCTAAGAGCAGGCATGCACAGCTCTGTGCACCTGGAGGAG
GCTGGCATTCTACGGGCTGGTCCAGGAGGGCCTGATCAGCAGTGGCCAACAGTATCCAGTGCAACACCAG
AACCTGAGGCAGGAGAGGTGTCCCCTCCGGTGGGCGCCGGTGTCAACAGCAACAGCTGGACCTTTAAATA
CGGACCAGGCAACCCCAAACAGTCCGGTCCCGAACCTAAAAAGCAGACCCAGGTTTCCTTTCTCCCCCGC
CGCAAAGGAGAAGCTTCCCAGCCCCGCCAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR209688 protein sequence
Red=Cloning site Green=Tags(s)

MEFSWGSGQESQRLLLSFLLLAIWEAGNSQIHYSIPEEAKHGTFVGRIAQDLGLELTELVPRLFRVASKD
RGDLLEVNLQNGILFVNSRIDREELCGRSAECSIHLEVIVDRPLQVFHVEVEVRDINDNPPRFPTTQKNL
FIAESRPLDTWFPLEGASDADIGINAVLTYRLSPNDYFSLEKPSNDERVKGLGLVLRKSLDREETPEIIL
VLTVTDGGKPELTGSVQLLITVLDANDNAPVFDRSLYTVKLPENVPNGTLVVKVNASDLDEGVNGDIMYS
FSTDISPNVKYKFHIDPVSGEIIVKGYIDFEECKSYEILIEGIDKGQLPLSGHCKVIVQVEDINDNVPEL
EFKSLSLPIRENSPVGTVIALISVSDRDTGVNGQVTCSLTSHVPFKLVSTFKNYYSLVLDSALDRETTAD
YKVVVTARDGGSPSLWATASVSVEVADVNDNAPVFAQPEYTVFVKENNPPGAHIFTVSAMDADAQENALV
SYSLVERRVGERLLSSYVSVHAESGKVFALQPLDHEELELLRFQPRQPNPDWRYSASLRAGMHSSVHLEE
AGILRAGPGGPDQQWPTVSSATPEPEAGEVSPPVGAGVNSNSWTFKYGPGNPKQSGPEPKKQTQVSFLPR
RKGEASQPRQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC060211
ORF Size 1920 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC060211, AAH60211
RefSeq Size 4166 bp
RefSeq ORF 1922 bp
Locus ID 12936
MW 70.4 kDa
Gene Summary Calcium-dependent cell-adhesion protein involved in cells self-recognition and non-self discrimination (Probable). Thereby, it is involved in the establishment and maintenance of specific neuronal connections in the brain (PubMed:27161523).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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