Map4k1 (BC005433) Mouse Tagged ORF Clone

CAT#: MR209699

  • TrueORF®

Map4k1 (Myc-DDK-tagged) - Mouse mitogen activated protein kinase kinase kinase kinase 1 (cDNA clone MGC:5927 IMAGE:3494470)

ORF Plasmid: DDK tGFP



  "BC005433" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 4,776.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms Hpk1; mHPK1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR209699 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCTTGTGGACCCCGACATTTTTAATAAGGACCCTCGAGAACACTATGATCTACTGCAACGACTGG
GTGGTGGAACTTATGGAGAAGTATTCAAGGCTCGCGACAAGGTGTCTAAGGACCTGGTGGCTTTGAAGAT
GGTGAAGATGGAGCCTGATGATGACGTCGCCACCCTTCAGAAGGAGATTCTCATGCTGAAAACTTGCCGG
CACGCCAACATCGTGGCTTACCATGGCAGTTACCTCTGGCTGCAGAAGCTGTGGATCTGCATGGAATTCT
GTGGAGCCGGTTCTCTCCAGGACATCTACCAAGTGACTGGCTCTCTGTCCGAGCTCCAGATCAGCTATGT
GTGCCGGGAAGTGCTTCAGGGACTGGCCTACCTGCATTCAGAGAAGAAGATACATCGAGACATCAAGGGA
GCCAACATCCTCATCAACGACTGCGGGGAGGTTAAGCTGGCTGACTTTGGGATCTCAGCCCAGATTGGGG
CGACACTGGCCCGGCGCCTTTCTTTCATTGGGACACCGTACTGGATGGCTCCAGAGGTGGCAGCGGTGGC
CCTGAAGGGTGGATACAATGAGCTGTGCGACATCTGGTCTCTTGGCATCACAGCCATCGAGCTGGCTGAG
CTTCAGCCCCCTCTCTTTGATGTGCACCCTCTCAGGGTTCTCTTCCTCATGACCAAGAGCGGGTACCAGC
CTCCTCGGCTGAAGGAAAAGAGCAGATGGTCATCTTCCTTCCACAACTTTGTCAAAGTCACCCTCACTAA
GAACTCCAAGAAACGCCCCAGTGCCACCAAGATGCTCAGTCATCAGCTGGTGTCCCAGCCAGGGCTGAAC
AGAGGCCTGATCTTGGATCTTCTTGACAAAATGAAGAATCCTGGGAAGGGGCTGCCTGTTGATATTGAAG
ATGAGGAGCCTGAGCCACCCCCTGCCATCCCTCGGCGGATCAGATCTACCTACCGGGCCAGCTCACTAGG
GATCCCAGACGCAGACTGCTGCCGGCGGCAAATGGAGTTCCAGAGGCCCAGATGCGTGGACTGCAGACCT
CAGGCTGAAACTGTTCGCTTATATCCCCCTGCACACTTCGGGAGCACCAGTCCCAGGAGTCAGCTGTCAG
ATTCTGATGACGACTATGATGATGTGGACATCCCTGCCCCTTCAGAGAACATACCTCCTCCACTGCCCCC
CAAGCCCAAGTTTCGGTCTCCATCAGATGATGGTTCTGGAGGAATTAGGGATGATGGGCAATTGAGTCCA
GGGGTTCTGGTTCGATGTGCAAGTGGGCCCCCTCCACGTACTCCCCGACCTGGACCTCCCCCAGCGACCC
GCAGCCCTCACCTCACAGCTCGCTCAGATCCCTCATTGTGGAACCCAGCTGCTCCGGAGCCAGGCCAGCC
CCCACTCGTACCCCCAAGGAAGGAAAAGATGAGGGGAAAGATGGAGAACGAGAAGCGCAGAGAGAAATAT
CCCCTTCTTGTGAAATTATTCAACGGCTGTCCCCTCCAGATCCACAGCACAGCTGCCTGGACACATCCCT
CTACAAAAGATCAGAACCTGCTCCTGGGGGCAGAGGAAGGCATTTTCATACTGAACCGGAATGATCAGGA
GGCCACGCTGGAAATGATCTTCCCTGGCCGCACAACCTGGCTCTATTGCATCAACAACCTCCTCATGTCT
CTCTCAGGGAAGACACCCTACCTGTATTCTCATAGCATCCTGGGCCTGCTTGAAAGGAAAGACGGCAGAA
CAGGAAGCCCCATTGCTCACATTAGCCCTCACCGGTTACTGGCAAGAAAGAACATGGTCTCCAGTAAGAT
CCAGGACACCAAAGGCTGCCGGGCTTGCTGTGTGGGTGAGAGCCAGCTTCCCTCCGGGTGCAGAGTGATG
GGGCGGGCCGGTGCCTGGCCAGTGTCTACTCAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR209699 protein sequence
Red=Cloning site Green=Tags(s)

MALVDPDIFNKDPREHYDLLQRLGGGTYGEVFKARDKVSKDLVALKMVKMEPDDDVATLQKEILMLKTCR
HANIVAYHGSYLWLQKLWICMEFCGAGSLQDIYQVTGSLSELQISYVCREVLQGLAYLHSEKKIHRDIKG
ANILINDCGEVKLADFGISAQIGATLARRLSFIGTPYWMAPEVAAVALKGGYNELCDIWSLGITAIELAE
LQPPLFDVHPLRVLFLMTKSGYQPPRLKEKSRWSSSFHNFVKVTLTKNSKKRPSATKMLSHQLVSQPGLN
RGLILDLLDKMKNPGKGLPVDIEDEEPEPPPAIPRRIRSTYRASSLGIPDADCCRRQMEFQRPRCVDCRP
QAETVRLYPPAHFGSTSPRSQLSDSDDDYDDVDIPAPSENIPPPLPPKPKFRSPSDDGSGGIRDDGQLSP
GVLVRCASGPPPRTPRPGPPPATRSPHLTARSDPSLWNPAAPEPGQPPLVPPRKEKMRGKMENEKRREKY
PLLVKLFNGCPLQIHSTAAWTHPSTKDQNLLLGAEEGIFILNRNDQEATLEMIFPGRTTWLYCINNLLMS
LSGKTPYLYSHSILGLLERKDGRTGSPIAHISPHRLLARKNMVSSKIQDTKGCRACCVGESQLPSGCRVM
GRAGAWPVSTQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC005433
ORF Size 1923 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC005433, AAH05433
RefSeq Size 2783 bp
RefSeq ORF 1925 bp
Locus ID 26411
MW 71.2 kDa
Gene Summary Serine/threonine-protein kinase, which may play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway. May play a role in hematopoietic lineage decisions and growth regulation. Able to autophosphorylate.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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