Ngly1 (NM_021504) Mouse Tagged ORF Clone
CAT#: MR209772
- TrueORF®
Ngly1 (Myc-DDK-tagged) - Mouse N-glycanase 1 (Ngly1)
ORF Plasmid: tGFP
"NM_021504" in other vectors (4)
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CNY 4,856.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 1110002C09Rik; Png1; PNGase |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR209772 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTCGGCCACACTGGGCAGCTCGTCGAGCTCTGCGTCCCCGGCCGTGGCCGAGCTATGCCAGAACA CCCCGGAGACCTTTCTGGAGGCCTCCAAGCTGCTGCTCACCTACGCCGACAACATCCTGAGAAACCCCAG TGATGAAAAATACAGATCCATCCGTATTGGGAACACAGCGTTTTCTACTAGACTCTTGCCTGTCAGAGGA GCTGTTGAGTGTTTATTTGAAATGGGCTTTGAAGAGGGAGAAACGCATCTTATCTTTCCTAAAAAAGCTT CAGTGGAACAGCTACAGAAAATCCGTGATCTGATTGCCATAGAGAGAAGCAGCAGACTGGACGGATCCAG TAAGAAGGTGCAATTCTCTCAGCACCCGGCTGCTGCCAAGCTTCCTCTGGAGCAGTCTGAAGATCCTGCT GGGTTAATACGGCACTCAGGAAACCAAACAGGGCAGCTGCCAAGCCTACCCTCTGCTCCAATGGTTGTTG GTGATTCAACCATCTTAAAAGTTCTTCAGTCCAACATTCAGCATGTGCAGCTCTATGAAAACCCTGTCCT CCAGGAGAAAGCATTGACTTGTATCCCAGTCAGTGAACTAAAAAGAAAAGCTCAAGAAAAGTTATTCAGA GCTAGAAAGTTAGATAAAGGTACTAATGTAAGTGATGAGGACTTCCTTCTACTGGAGCTCCTACACTGGT TTAAAGAGGAGTTTTTTCGCTGGGTGAATAACATTGTGTGCAGCAAATGTGGTGGAGAGACTAGATCTAG AGATGAGGCATTGCTGCCCAATGATGATGAACTGAAGTGGGGTGCAAAGAATGTGGAGAATCATTACTGT GATGCCTGCCAGTTGAGCAACCGCTTCCCAAGATATAACAACCCCGAGAAACTTTTGGAAACAAGATGTG GACGCTGTGGTGAATGGGCCAATTGTTTTACTCTGTGCTGCCGTGCATTAGGGTTTGAAGCTCGATATGT GTGGGATTACACAGACCATGTTTGGACAGAAGTCTATTCTCCATCTCAGCAGCGGTGGCTACACTGTGAT GCATGTGAGGATGTCTGTGACAAACCCCTCCTTTATGAAATAGGATGGGGCAAGAAGCTTTCCTATATAA TAGCATTCTCTAAAGATGAGGTAGTTGATGTCACCTGGCGATACTCTTGTAAACATGATGAGGTGATGTC CAGGAGGACCAAGGTTAAAGAAGAGTTACTTCGAGAAACTATTAATGGGCTTAATAAGCAGAGACAGCTA TCATTATCAGAAAGCAGAAGGAAAGAACTTCTCCAGAGGATAATTGTGGAGCTTGTTGAATTTATATCTC CTAAAACCCCAAGACCTGGAGAACTTGGTGGAAGGGTATCCGGGTCATTGGCTTGGAGAGTGGCCCGAGG TGAGACGGGTCTAGAGAGAAAGGAGATTCTGTTCATCCCTTCTGAAAATGAAAAGATTTCTAAGCAGTTT CACCTCCGTTATGATATTGTGAGAGACCGTTACATTCGTGTCTCAGACAACAACATAAACATTTCTGGAT GGGAGAATGGTGTGTGGAAAATGGAATCCATATTCAGAAAAGTCGAGAAAGACTGGAACATGGTTTATTT GGCCCGAAAAGAAGGATCATCTTTTGCTTATATTTCCTGGAAGTTTGAATGTGGGTCAGCTGGTCTAAAA GTAGATACTGTTTCCATCCGCACAAGTAGCCAAAGCTTTGAGTCTGGATCAGTGAGGTGGAAACTGCGAT CTGAGACAGCTCAAGTCAACCTGCTGGGAGATAAAAATCTACGTTCCTATAATGATTTTTCTGGTGCCAC AGAGGTTACATTAGAAGCAGAGTTAAGCAGAGGAGATGGAGACGTTGCTTGGCAACATACCCAACTGTTT AGACAAAGCTTAAATGACAGTGGAGAAAATGGCTTGGAAATAATTATAACGTTCAATGACCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR209772 protein sequence
Red=Cloning site Green=Tags(s) MASATLGSSSSSASPAVAELCQNTPETFLEASKLLLTYADNILRNPSDEKYRSIRIGNTAFSTRLLPVRG AVECLFEMGFEEGETHLIFPKKASVEQLQKIRDLIAIERSSRLDGSSKKVQFSQHPAAAKLPLEQSEDPA GLIRHSGNQTGQLPSLPSAPMVVGDSTILKVLQSNIQHVQLYENPVLQEKALTCIPVSELKRKAQEKLFR ARKLDKGTNVSDEDFLLLELLHWFKEEFFRWVNNIVCSKCGGETRSRDEALLPNDDELKWGAKNVENHYC DACQLSNRFPRYNNPEKLLETRCGRCGEWANCFTLCCRALGFEARYVWDYTDHVWTEVYSPSQQRWLHCD ACEDVCDKPLLYEIGWGKKLSYIIAFSKDEVVDVTWRYSCKHDEVMSRRTKVKEELLRETINGLNKQRQL SLSESRRKELLQRIIVELVEFISPKTPRPGELGGRVSGSLAWRVARGETGLERKEILFIPSENEKISKQF HLRYDIVRDRYIRVSDNNINISGWENGVWKMESIFRKVEKDWNMVYLARKEGSSFAYISWKFECGSAGLK VDTVSIRTSSQSFESGSVRWKLRSETAQVNLLGDKNLRSYNDFSGATEVTLEAELSRGDGDVAWQHTQLF RQSLNDSGENGLEIIITFNDL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_021504 |
ORF Size | 1956 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_021504.3 |
RefSeq Size | 2901 bp |
RefSeq ORF | 1956 bp |
Locus ID | 59007 |
UniProt ID | Q9JI78 |
MW | 74.3 kDa |
Gene Summary | Specifically deglycosylates the denatured form of N-linked glycoproteins in the cytoplasm and assists their proteasome-mediated degradation. Cleaves the beta-aspartyl-glucosamine (GlcNAc) of the glycan and the amide side chain of Asn, converting Asn to Asp. Prefers proteins containing high-mannose over those bearing complex type oligosaccharides. Can recognize misfolded proteins in the endoplasmic reticulum that are exported to the cytosol to be destroyed and deglycosylate them, while it has no activity toward native proteins. Deglycosylation is a prerequisite for subsequent proteasome-mediated degradation of some, but not all, misfolded glycoproteins.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC202185 | Ngly1 (untagged) - Mouse N-glycanase 1 (Ngly1), (10ug) |
CNY 4,856.00 |
|
MG209772 | Ngly1 (tGFP-tagged) - Mouse N-glycanase 1 (Ngly1) |
CNY 5,040.00 |
|
MR209772L3 | Lenti ORF clone of Ngly1 (Myc-DDK-tagged) - Mouse N-glycanase 1 (Ngly1) |
CNY 6,460.00 |
|
MR209772L4 | Lenti ORF clone of Ngly1 (mGFP-tagged) - Mouse N-glycanase 1 (Ngly1) |
CNY 6,460.00 |