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Pdzrn3 (BC010329) Mouse Tagged ORF Clone
CAT＃: MR209785
- TrueORF®
Pdzrn3 (Myc-DDK-tagged) - Mouse PDZ domain containing RING finger 3 (cDNA clone MGC:11956 IMAGE:3600603)
ORF Plasmid: tGFP
"BC010329" in other vectors (4)
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CNY 6,560.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1110020C07Rik; AI429718; AL023082; LNX3; SEMACAP3; Semcap3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR209785 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACAGAGAAGAGCTGGAGCTGGAGGAAGTGGGCCTCTACAGGATGAACAGCCAGGACAAGCTGGGTC TCACTGTGTGCTACCGGACGGATGACGAAGATGACATTGGGATATATATAAGTGAGATTGACCCTAACAG CATTGCAGCCAAAGATGGACGGATCCGTGAAGGAGATCGCATCATCCAGATTAATGGCATCGAAGTACAG AACCGTGAAGAGGCCGTGGCGCTTCTAACCAGTGAAGAAAACAAGAACTTTTCATTGCTGATTGCAAGGC CTGAGCTCCAGCTGGATGAGGGCTGGATGGATGACGACAGGAACGACTTCCTGGATGACTTACACATGGA CATGCTGGAGGAACAGCATCACCAGGCCATGCAGTTCACAGCCAGTGTGCTCCAGCAGAAGAAGCATGAG GAAGATGGTGGGACCACAGACACAGCCACCATCTTATCCAACCAGCACGAGAAAGACAGTGGTGTGGGGC GAACGGATGAGAGCACCCGCAATGATGAGAGCTCAGAACAGGAGAATAACGGTGAAGATGCTACGGCATC CGCCAACCCGCTGGCGGGCCAGAGGAAGCTGACCTGTAGCCAGGACACCCTGGGCAGTGGCGACCTGCCT TTCAGCAATGAGTCCTTCATCTCTGCGGACTGTACCGATGTGGACTACCTGGGCATCCCAGAGGATGAGT GCGAGCGTTTTCGTGAACTGCTGGAGCTCAAGTGCCAAGTGCAGAGCGCCAGCCCCTACAGCCTGTACTA CCCTAGCAGCCCGCTGGATGCTGCTGGCAAGAGTGACCCCGAGAGCGTGGACAAGGAGCTGGAGCTACTC AATGAGGAGTTGCGCAGCATCGAGCTAGAGTGCCTGAGCATCGTGCGTGCGCACAAGATGCAGCAGCTCA AGGAACAGTACCGTGAGTCCTGGATGCTGCACCACAGTGGCTTCCGCAACTACAACACCAGTGTGGACGT GCGCCGCCATGAGCTCTCGGACATCACTGAGCTGCCGGAGAAGTCAGACAAGGATAGTTCAAGCGCCTAC AACACGGGGGAGAGCTGCAGGAGTACCCCACTCACCCTGGAGATCTCTCCGGACAACTCCCTGCGGAGAG TGGCCGAGGGCAGTAGTGAAGGGGCCACAGCTAACATCGAAGCTTACAGGCCATCCCCCAAGAATCTACT CGCCATCACTGAGGACCCTGAAGTAAGCACCCCAAGCTACAACCCTAGCTCCAAAGAGCTGGACCCCAGC CAGGCTTTGGAGATCAAAGAACGCCGAGGTAGCGATGGCAGCAGGAGCCCCACCGCCAGCCCAAAGCTGG GCAATGCCTACCTGCCCTCGTACCACCACTCCCCCTACAAACACGCGCACATCCCAGCTCACGCGCAACA CTACCAGAGCTACATGCACCTGATCCAGCAGAAGTCAGCAGTGGAGTATGCGCAGAGCCAGATGAGCCTG GTGAGCATGTGCAAGGACTTGAACTCTTCTAACTCTGCGGAACCCAGGATGGAATGGAAAGTGAAGATCC GCAGTGACGGGACGCGCTACATCACCAAGAGGCCGGTGCGTGACAGACTGCTGCGGGAGCGTGCGCTGAA GATCCGCGAGGAGCGCAGTGGCCTGACCACGGATGATGACGCCATGAGTGAGATGAAGATGGGGCGCTAC TGGAGCAAGGAGGAGCGCAAGCAACATCTGGTGAAGGCCAAGGAGCAGAGGCGGAGGCGCGAGTTTATGA TGCAGAGCAGGCTGGATTGTCTGAAGGAGCAGCAGGCCTCCGACGACAGGAAGGAGATGAACATCCTGGA ACTGAGCCACAAAAAGATGATGAAGAAGAGGAACAAAAAGATCTTTGACAATTGGATGACTATCCAAGAA CTCTTAACCCACGGCACAAAATCGCCAGACGGCACTAGGGTATACAATTCCTTCCTGTCGGTGACTACTG TA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR209785 protein sequence
Red=Cloning site Green=Tags(s) MDREELELEEVGLYRMNSQDKLGLTVCYRTDDEDDIGIYISEIDPNSIAAKDGRIREGDRIIQINGIEVQ NREEAVALLTSEENKNFSLLIARPELQLDEGWMDDDRNDFLDDLHMDMLEEQHHQAMQFTASVLQQKKHE EDGGTTDTATILSNQHEKDSGVGRTDESTRNDESSEQENNGEDATASANPLAGQRKLTCSQDTLGSGDLP FSNESFISADCTDVDYLGIPEDECERFRELLELKCQVQSASPYSLYYPSSPLDAAGKSDPESVDKELELL NEELRSIELECLSIVRAHKMQQLKEQYRESWMLHHSGFRNYNTSVDVRRHELSDITELPEKSDKDSSSAY NTGESCRSTPLTLEISPDNSLRRVAEGSSEGATANIEAYRPSPKNLLAITEDPEVSTPSYNPSSKELDPS QALEIKERRGSDGSRSPTASPKLGNAYLPSYHHSPYKHAHIPAHAQHYQSYMHLIQQKSAVEYAQSQMSL VSMCKDLNSSNSAEPRMEWKVKIRSDGTRYITKRPVRDRLLRERALKIREERSGLTTDDDAMSEMKMGRY WSKEERKQHLVKAKEQRRRREFMMQSRLDCLKEQQASDDRKEMNILELSHKKMMKKRNKKIFDNWMTIQE LLTHGTKSPDGTRVYNSFLSVTTV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC010329 |
| ORF Size | 1962 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC010329, AAH10329 |
| RefSeq Size | 2921 bp |
| RefSeq ORF | 1964 bp |
| Locus ID | 55983 |
| MW | 74.7 kDa |
| Gene Summary | E3 ubiquitin-protein ligase. Plays an important role in regulating the surface level of MUSK on myotubes. Mediates the ubiquitination of MUSK, promoting its endocytosis and lysosomal degradation. Might contribute to terminal myogenic differentiation.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218108 | Pdzrn3 (untagged) - Mouse PDZ domain containing RING finger 3 (cDNA clone MGC:11956 IMAGE:3600603), (10ug) |
CNY 6,940.00 |
|
| MG209785 | Pdzrn3 (tGFP-tagged) - Mouse PDZ domain containing RING finger 3 (cDNA clone MGC:11956 IMAGE:3600603) |
CNY 7,220.00 |
|
| MR209785L3 | Lenti ORF clone of Pdzrn3 (Myc-DDK-tagged) - Mouse PDZ domain containing RING finger 3 (cDNA clone MGC:11956 IMAGE:3600603) |
CNY 8,460.00 |
|
| MR209785L4 | Lenti ORF clone of Pdzrn3 (mGFP-tagged) - Mouse PDZ domain containing RING finger 3 (cDNA clone MGC:11956 IMAGE:3600603) |
CNY 8,460.00 |
