Sema7a (NM_011352) Mouse Tagged ORF Clone
CAT#: MR209876
- TrueORF®
Sema7a (Myc-DDK-tagged) - Mouse sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin) 7A (Sema7a)
ORF Plasmid: tGFP
"NM_011352" in other vectors (4)
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CNY 7,416.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 2900057C09Rik; CDw108; H-Sema-L; M-Sema-L; Semal |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR209876 representing NM_011352
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGCCTCCTCCTCCCGGACGTGCCGCCCCCAGCGCACCGCGCGCCCGCGTCCTCAGCCTGCCGGCTC GGTTCGGGCTCCCGCTGCGGCTGCGGCTTCTGCTGGTGTTCTGGGTGGCCGCCGCCTCCGCCCAAGGCCA CTCGAGGAGCGGACCCCGCATCTCCGCCGTCTGGAAAGGGCAGGACCATGTGGACTTTAGCCAGCCTGAG CCACACACCGTGCTTTTCCATGAGCCGGGCAGCTTCTCTGTCTGGGTGGGTGGACGTGGCAAGGTCTACC ACTTCAACTTCCCCGAGGGCAAGAATGCCTCTGTGCGCACGGTGAACATCGGCTCCACAAAGGGGTCCTG TCAGGACAAACAGGACTGTGGGAATTACATCACTCTTCTAGAAAGGCGGGGTAATGGGCTGCTGGTCTGT GGCACCAATGCCCGGAAGCCCAGCTGCTGGAACTTGGTGAATGACAGTGTGGTGATGTCACTTGGTGAGA TGAAAGGCTATGCCCCCTTCAGCCCGGATGAGAACTCCCTGGTTCTGTTTGAAGGAGATGAAGTGTACTC TACCATCCGGAAGCAGGAATACAACGGGAAGATCCCTCGGTTTCGACGCATTCGGGGCGAGAGTGAACTG TACACAAGTGATACAGTCATGCAGAACCCACAGTTCATCAAGGCCACCATTGTGCACCAAGACCAAGCCT ATGATGATAAGATCTACTACTTCTTCCGAGAAGACAACCCTGACAAGAACCCCGAGGCTCCTCTCAATGT GTCCCGAGTAGCCCAGTTGTGCAGGGGGGACCAGGGTGGTGAGAGTTCGTTGTCTGTCTCCAAGTGGAAC ACCTTCCTGAAAGCCATGTTGGTCTGCAGCGATGCAGCCACCAACAGGAACTTCAATCGGCTGCAAGATG TCTTCCTGCTCCCTGACCCCAGTGGCCAGTGGAGAGATACCAGGGTCTATGGCGTTTTCTCCAACCCCTG GAACTACTCAGCTGTCTGCGTGTATTCGCTTGGTGACATTGACAGAGTCTTCCGTACCTCATCGCTCAAA GGCTACCACATGGGCCTTCCCAACCCTCGACCTGGCATGTGCCTCCCAAAAAAGCAGCCCATACCCACAG AAACCTTCCAGGTAGCTGATAGTCACCCAGAGGTGGCTCAGAGGGTGGAACCTATGGGGCCACTGAAGAC ACCATTGTTCCATTCTAAGTACCATTACCAGAAAGTGGTCGTTCACCGCATGCAAGCCAGCAATGGAGAG ACCTTCCATGTGCTTTATCTAACCACAGACAGGGGCACCATTCACAAGGTGGTGGAATCAGGGGACCAGG ACCATAGCTTTGTCTTCAATATCATGGAGATCCAGCCCTTTCACCGTGCAGCGGCCATCCAGGCTATATC ATTGGATGCTGACCGGCGGAAGCTCTATGTGACCTCCCAGTGGGAAGTGAGCCAGGTACCCCTGGACATG TGTGAGGTCTACAGCGGGGGCTGCCATGGCTGCCTCATGTCCCGAGACCCCTACTGTGGCTGGGACCAGG ACCGCTGCGTGTCTATCTACAGCTCCCAACGATCAGTGCTGCAGTCCATTAATCCAGCGGAGCCACACAG AGAGTGTCCCAACCCTAAACCAGACGAGGCTCCATTGCAGAAGGTTTCCCTGGCTCGGAACTCTCGATAC TACCTGACCTGCCCCATGGAGTCCCGCCACGCCACTTACTTATGGCGCCATGAGGAGAATGTGGAACAGA GCTGTGAGCCAGGCCACCAAAGCCCTAGCTGCATCCTGTTCATTGAGAACCTCACGGCCCGTCAGTATGG CCACTACCGCTGCGAGGCCCAAGAGGGCTCCTACCTCCGTGAGGCTCAACACTGGGAGCTGCTGCCAGAG GACAGAGCACTGGCCGAACAACTAATGGGCCATGCCCGGGCCCTGGCCGCCTCCTTCTGGCTGGGGGTCC TGCCCACACTCATACTTGGTCTGCTGGTTCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR209876 representing NM_011352
Red=Cloning site Green=Tags(s) MTPPPPGRAAPSAPRARVLSLPARFGLPLRLRLLLVFWVAAASAQGHSRSGPRISAVWKGQDHVDFSQPE PHTVLFHEPGSFSVWVGGRGKVYHFNFPEGKNASVRTVNIGSTKGSCQDKQDCGNYITLLERRGNGLLVC GTNARKPSCWNLVNDSVVMSLGEMKGYAPFSPDENSLVLFEGDEVYSTIRKQEYNGKIPRFRRIRGESEL YTSDTVMQNPQFIKATIVHQDQAYDDKIYYFFREDNPDKNPEAPLNVSRVAQLCRGDQGGESSLSVSKWN TFLKAMLVCSDAATNRNFNRLQDVFLLPDPSGQWRDTRVYGVFSNPWNYSAVCVYSLGDIDRVFRTSSLK GYHMGLPNPRPGMCLPKKQPIPTETFQVADSHPEVAQRVEPMGPLKTPLFHSKYHYQKVVVHRMQASNGE TFHVLYLTTDRGTIHKVVESGDQDHSFVFNIMEIQPFHRAAAIQAISLDADRRKLYVTSQWEVSQVPLDM CEVYSGGCHGCLMSRDPYCGWDQDRCVSIYSSQRSVLQSINPAEPHRECPNPKPDEAPLQKVSLARNSRY YLTCPMESRHATYLWRHEENVEQSCEPGHQSPSCILFIENLTARQYGHYRCEAQEGSYLREAQHWELLPE DRALAEQLMGHARALAASFWLGVLPTLILGLLVH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_011352 |
ORF Size | 1992 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_011352.3 |
RefSeq Size | 3290 bp |
RefSeq ORF | 1995 bp |
Locus ID | 20361 |
UniProt ID | Q9QUR8 |
MW | 75.4 kDa |
Gene Summary | Plays an important role in integrin-mediated signaling and functions both in regulating cell migration and immune responses. Promotes formation of focal adhesion complexes, activation of the protein kinase PTK2/FAK1 and subsequent phosphorylation of MAPK1 and MAPK3. Promotes production of proinflammatory cytokines by monocytes and macrophages. Plays an important role in modulating inflammation and T-cell-mediated immune responses. Promotes axon growth in the embryonic olfactory bulb. Promotes attachment, spreading and dendrite outgrowth in melanocytes.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC220227 | Sema7a (untagged) - Mouse sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin) 7A (Sema7a), (10ug) |
CNY 7,424.00 |
|
MG209876 | Sema7a (tGFP-tagged) - Mouse sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin) 7A (Sema7a) |
CNY 9,016.00 |
|
MR209876L3 | Lenti ORF clone of Sema7a (Myc-DDK-tagged) - Mouse sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin) 7A (Sema7a) |
CNY 9,816.00 |
|
MR209876L4 | Lenti ORF clone of Sema7a (mGFP-tagged) - Mouse sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin) 7A (Sema7a) |
CNY 8,550.00 |