Slc5a1 (NM_019810) Mouse Tagged ORF Clone
CAT#: MR209884
- TrueORF®
Slc5a1 (Myc-DDK-tagged) - Mouse solute carrier family 5 (sodium/glucose cotransporter), member 1 (Slc5a1)
ORF Plasmid: tGFP
"NM_019810" in other vectors (4)
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CNY 7,424.00
Cited in 3 publications. |
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Sglt1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR209884 representing NM_019810
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACAGTAGCACCTTGAGCCCCGCGGTTACTGCCACCGATGCACCCATCCCGTCCTACGAGCGCATTC GCAATGCGGCTGACATCTCAGTCATCGTCATCTACTTCGTGGTGGTGATGGCTGTCGGGCTGTGGGCCAT GTTTTCCACTAATCGTGGTACCGTTGGAGGCTTCTTCCTGGCAGGCCGAAGTATGGTGTGGTGGCCGATT GGAGCCTCTCTCTTTGCCAGTAACATTGGAAGTGGTCACTTTGTGGGGCTGGCAGGCACAGGAGCAGCCG CAGGCATTGCCATGGGTGGCTTTGAATGGAACGCCTTGGTTTTGGTGGTTGTGCTGGGCTGGATATTTGT CCCGATTTACATCAAGGCCGGGGTGGTGACGATGCCGGAGTATCTGCGGAAGCGGTTTGGAGGCAAGAGA ATTCAGATCTACCTTTCCGTTCTGTCCCTGCTCCTCTACATCTTCACAAAGATCTCTGCAGACATCTTCT CCGGGGCTATATTCATCAATCTAGCCTTGGGCCTGGATATATACTTGGCCATTTTCATCCTGCTAGCAAT CACTGCCCTTTATACAATCACAGGGGGGCTGGCAGCTGTAATTTACACAGATACCCTGCAGACCGCGATC ATGCTGGTGGGGTCTTTTATCCTGACTGGGTTTGCTTTCAATGAAGTAGGAGGATATGAAGCCTTCATGG ACAAGTACATGAAAGCTATTCCAACTAAGGTTTCTAACGGAAATTTTACCGCCAAGGAAGAATGCTACAC ACCGAGGGCTGACTCATTCCACATATTCAGAGACCCCATTACTGGAGACATGCCGTGGCCTGGGCTCATT TTTGGCCTGGCCATCCTCGCCCTGTGGTACTGGTGTACGGATCAGGTCATTGTGCAGCGATGCCTCTCGG CCAAGAACATGTCTCACGTGAAGGCTGGCTGTACCCTGTGTGGCTATCTGAAGCTACTGCCCATGTTCCT CATGGTGATGCCAGGAATGATCAGCCGGATTCTATATACAGAAAAAATCGCCTGTGTCCTCCCTGAAGAA TGTCAGAAATACTGCGGCACACCAGTGGGCTGTACCAACATCGCCTACCCCACGCTCGTGGTGGAACTCA TGCCTAATGGACTGCGAGGCCTGATGCTGTCTGTCATGATGGCTTCCCTCATGAGCTCCTTGACCTCCAT CTTCAACAGCGCCAGTACTCTCTTCACCATGGACATCTACACCAAGATCCGGAAGAAGGCATCTGAGAAG GAGCTCATGATTGCAGGAAGGTTGTTCATCCTCGTGCTGATTGGCATCAGCATTGCCTGGGTACCCATTG TACAGTCTGCTCAAAGTGGGCAGCTTTTTGATTACATCCAGTCTATCACCAGTTACCTGGGGCCGCCCAT TGCAGCTGTCTTCCTGCTTGCCATTTTCTGCAAGAGAGTCAATGAACAAGGAGCCTTCTGGGGACTGATC CTGGGCTTCCTGATTGGAATCTCCCGTATGATCACTGAGTTTGCCTATGGAACTGGGAGCTGCATGGAGC CCAGCAACTGCCCCAAGATCATCTGTGGGGTCCACTATTTGTACTTTGCCATCATCCTCTTCGTCATCAG CGTCATCACCATCTTGATCATCTCCTTCCTCACCAAGCCCATTCCAGACGTGCACCTGTACCGTTTGTGT TGGAGTCTACGCAACAGCAAGGAAGAGCGAATCGACCTGGATGCAGGAGAGGAGGAGGACATCCCAGAGG ACTCCAAGGACACAATTGAAATAGACACAGAAGCCCCCCAGAAGAAGAAAGGATGCTTCAGGAGAGCATA TGACCTGTTCTGTGGGCTGGACCAAGATAAAGGACCAAAGATGACTAAGGAGGAGGAGGAAGCCATGAAG ATGAAGATGACAGACACTTCTGAGAAGCCTTTGTGGAGGACAGTGGTGAACATCAACGGTATTATCCTGC TGGCGGTAGCTGTCTTCTGCCATGCCTATTTTGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR209884 representing NM_019810
Red=Cloning site Green=Tags(s) MDSSTLSPAVTATDAPIPSYERIRNAADISVIVIYFVVVMAVGLWAMFSTNRGTVGGFFLAGRSMVWWPI GASLFASNIGSGHFVGLAGTGAAAGIAMGGFEWNALVLVVVLGWIFVPIYIKAGVVTMPEYLRKRFGGKR IQIYLSVLSLLLYIFTKISADIFSGAIFINLALGLDIYLAIFILLAITALYTITGGLAAVIYTDTLQTAI MLVGSFILTGFAFNEVGGYEAFMDKYMKAIPTKVSNGNFTAKEECYTPRADSFHIFRDPITGDMPWPGLI FGLAILALWYWCTDQVIVQRCLSAKNMSHVKAGCTLCGYLKLLPMFLMVMPGMISRILYTEKIACVLPEE CQKYCGTPVGCTNIAYPTLVVELMPNGLRGLMLSVMMASLMSSLTSIFNSASTLFTMDIYTKIRKKASEK ELMIAGRLFILVLIGISIAWVPIVQSAQSGQLFDYIQSITSYLGPPIAAVFLLAIFCKRVNEQGAFWGLI LGFLIGISRMITEFAYGTGSCMEPSNCPKIICGVHYLYFAIILFVISVITILIISFLTKPIPDVHLYRLC WSLRNSKEERIDLDAGEEEDIPEDSKDTIEIDTEAPQKKKGCFRRAYDLFCGLDQDKGPKMTKEEEEAMK MKMTDTSEKPLWRTVVNINGIILLAVAVFCHAYFA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_019810 |
ORF Size | 1995 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_019810.2, NM_019810.3, NM_019810.4, NP_062784.3 |
RefSeq Size | 3933 bp |
RefSeq ORF | 1998 bp |
Locus ID | 20537 |
UniProt ID | Q8C3K6 |
MW | 73.8 kDa |
Gene Summary | Actively transports glucose into cells by Na(+) cotransport with a Na(+) to glucose coupling ratio of 2:1. Efficient substrate transport in mammalian kidney is provided by the concerted action of a low affinity high capacity and a high affinity low capacity Na(+)/glucose cotransporter arranged in series along kidney proximal tubules.[UniProtKB/Swiss-Prot Function] |
Citations (3)
The use of this cDNA Clones has been cited in the following citations: |
---|
Concise and Stereodivergent Synthesis of Carbasugars Reveals Unexpected Structure-Activity Relationship (SAR) of SGLT2 Inhibition
,null,
Scientific Reports
,PubMed ID 28717146
[Slc5a1]
|
Sex-, Species-, and Tissue-Specific Metabolism of Empagliflozin in Male Mouse Kidney Forms an Unstable Hemiacetal Metabolite (M466/2) That Degrades to 4-Hydroxycrotonaldehyde, a Reactive and Cytotoxic Species
,Taub, ME;Ludwig-Schwellinger, E;Ishiguro, N;Kishimoto, W;Yu, H;Wagner, K;Tweedie, D;,
Chem. Res. Toxicol.
,PubMed ID 25489797
[SLC5A1]
|
KCNQ1, KCNE2, and Na + -Coupled Solute Transporters Form Reciprocally Regulating Complexes that Affect Neuronal Excitability
,null,
Science signaling
,PubMed ID 24595108
[Slc5a1]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC200185 | Slc5a1 (untagged) - Mouse solute carrier family 5 (sodium/glucose cotransporter), member 1 (Slc5a1), (10ug) |
CNY 7,440.00 |
|
MG209884 | Slc5a1 (tGFP-tagged) - Mouse solute carrier family 5 (sodium/glucose cotransporter), member 1 (Slc5a1) |
CNY 9,024.00 |
|
MR209884L3 | Lenti ORF clone of Slc5a1 (Myc-DDK-tagged) - Mouse solute carrier family 5 (sodium/glucose cotransporter), member 1 (Slc5a1) |
CNY 8,550.00 |
|
MR209884L4 | Lenti ORF clone of Slc5a1 (mGFP-tagged) - Mouse solute carrier family 5 (sodium/glucose cotransporter), member 1 (Slc5a1) |
CNY 8,550.00 |