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Rnf139 (NM_175226) Mouse Tagged ORF Clone
CAT＃: MR209908
- TrueORF®
Rnf139 (Myc-DDK-tagged) - Mouse ring finger protein 139 (Rnf139)
ORF Plasmid: tGFP
"NM_175226" in other vectors (4)
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CNY 4,896.00
CNY 5,800.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 4930555P18Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR209908 representing NM_175226
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGGTGGGGCCCCCGCAGCAGCAGGTGCGGATGGCCCAGCAGCAGGTCTGGGCGGCGCTCGAAG TGGCGCTCCGGGTGCCCTGCCTCTACATCATCGACGCCATCTTCAACTCCTACTACGATTCCAGCCAAAG CCGGTTCTGCATCGGGCTTCAGATCTTTCTCCGGCTCCTTGGTATAGTTGTATCCAGTATTGTTCTGATC TTGTCACAGCGATCACTTTTCAAGTTTTACATGTACAGCTCAGCCTTTCTATTAGCTGCAACTTCAGTGT TGGTGAATTACTATGCTGCTTTGCACATTGACTTCTATGGTGCCTACAACACGTCAGCGTTTGGAATCGA GTTGCTTCCTCGGAAAGGGCCCTCACTGTGGATGGCACTCATCGTTCTGCAGCTAACCTTTGGAATTGGA TATGTCACATTACTGCAGATTCAGTCCATCTATTCACAGTTAATGATTTTAAATATCTTGGTTCCTATAA TTGGCTTAATCACAGAGCTGCCATTACACATCAGAGAGACAGTAGTGCTCATGTCGTCCTTGATTCTCAT ATTTAACACAGTGCTTGTCTTGGCAGTGAAACTTAAGTGGTTTTACTACTCTACTCGGTATGTGTACTTG TTAGTGAGGCATATGTACCGAATTTATGGATTACAGTTACTGATGGAGGACACATGGAAGAGGATTCGTT TCCCAGACATACTTCGGGTCTTCTGGCTAACAAGAATTACAGCTCAGGCTACAGTGTTAATGTACATTTT AAGGATGGCAAATGAAACTGAGTCCTTCTTTATTTCTTGGGATGATTTCTGGGACGTCATTTGCAATCTA ATAATTAGTGGGTGTGATTCTACACTAACTGTACTAGGCATGAGTGCTGTAATTTCTTCAATAGCCCATT ATTTGGGCCTTGGAATATTGGCCTTTATTGGATCAACTGAAGAAGATGACAGGCGGCTTGGCTTTGTAGC TCCTGTTTTATTTTTCATTTTGGCTCTTCAGACTGGTTTAAGTGGGCTAAGACCTGAAGAGAGACTTATT CGCTTAAGTAGAAACATGTGCCTTTTATTAACTGCAGTCCTGCATTTCATCCATGGGATGACAGACCCTG TATTAATGTCGCTCAGTGCCTCCCATGTGTCATCTTTCCATAGACACTTTCCTGTGCTGTTTGTCTCTGC TTGCCTGTTCATTCTCCCTGTCTTACTCAGTTATGTCCTTTGGCATCACTATGCACTAAATACATGGCTG TTTGCAGTTACAGCCTTTTGTGTGGAACTCTGCTTGAAGGTAATCGTCTCCCTCACTGTCTACACGCTGT TCATGATTGATGGCTACTATAACGTCCTCTGGGAAAAGCTTGATGACTATGTCTACTTTGTTCGCTCCAC GGGCAATATTATCGAATTTATATTTGGAGTAGTAATGTTTGGGAATGGGGCCTATACCATGATGTTTGAG TCAGGAAGTAAAATTCGGGCTTGTATGATGTGCCTACATGCATATTTTAACATCTACTTACAAGTAAAAA ATGGCTGGAAGACATTCATGAATCGTAGAACTGCTGTTAAGAAAATTAATTCACTTCCTGAGATAAAAGG GAGCCACTTGCAAGAAATAGATGATGTCTGTGCAATCTGCTATCATGAGTTCACAACATCTGCTCGTATA ACACCGTGTAACCATTATTTCCATGCACTCTGCCTTCGGAAATGGCTGTACATTCAAGATACTTGTCCAA TGTGCCATCAGAAAGTGTACATCGAAGATGAGATCAAGGATAACTCAAATGCATCTAACAACAACGGATT TATTGCACCCAATGAAAATCCAAACCCAGAGGAGGCTTTAAGAGAGGATGCTGCTGGATCTGACCGAGAA CTGAACGAGGATGACAGTACAGACTGTGATGATGATGCTCAAAGAGAAAGAAACGGGGGGATTCAGCACA CAGGCGCAGCAGCAGCAGCCGCAGAATTTAATGATGACACTGAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR209908 representing NM_175226
Red=Cloning site Green=Tags(s) MAAVGPPQQQVRMAQQQVWAALEVALRVPCLYIIDAIFNSYYDSSQSRFCIGLQIFLRLLGIVVSSIVLI LSQRSLFKFYMYSSAFLLAATSVLVNYYAALHIDFYGAYNTSAFGIELLPRKGPSLWMALIVLQLTFGIG YVTLLQIQSIYSQLMILNILVPIIGLITELPLHIRETVVLMSSLILIFNTVLVLAVKLKWFYYSTRYVYL LVRHMYRIYGLQLLMEDTWKRIRFPDILRVFWLTRITAQATVLMYILRMANETESFFISWDDFWDVICNL IISGCDSTLTVLGMSAVISSIAHYLGLGILAFIGSTEEDDRRLGFVAPVLFFILALQTGLSGLRPEERLI RLSRNMCLLLTAVLHFIHGMTDPVLMSLSASHVSSFHRHFPVLFVSACLFILPVLLSYVLWHHYALNTWL FAVTAFCVELCLKVIVSLTVYTLFMIDGYYNVLWEKLDDYVYFVRSTGNIIEFIFGVVMFGNGAYTMMFE SGSKIRACMMCLHAYFNIYLQVKNGWKTFMNRRTAVKKINSLPEIKGSHLQEIDDVCAICYHEFTTSARI TPCNHYFHALCLRKWLYIQDTCPMCHQKVYIEDEIKDNSNASNNNGFIAPNENPNPEEALREDAAGSDRE LNEDDSTDCDDDAQRERNGGIQHTGAAAAAAEFNDDTD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_175226 |
| ORF Size | 2004 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_175226.4, NP_780435.1 |
| RefSeq Size | 4348 bp |
| RefSeq ORF | 2007 bp |
| Locus ID | 75841 |
| UniProt ID | Q7TMV1 |
| MW | 76.6 kDa |
| Gene Summary | E3-ubiquitin ligase; acts as a negative regulator of the cell proliferation through mechanisms involving G2/M arrest and cell death. Required for MHC class I ubiquitination in cells expressing the cytomegalovirus protein US2 before dislocation from the endoplasmic reticulum (ER). Affects SREBP processing by hindering the SREBP/SCAP complex translocation from the ER to the Golgi, thereby reducing SREBF2 target gene expression. Required for INSIG1 ubiquitination. May be required for EIF3 complex ubiquitination. May function as a signaling receptor.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220271 | Rnf139 (untagged) - Mouse ring finger protein 139 (Rnf139), (10ug) |
CNY 8,550.00 |
|
| MG209908 | Rnf139 (tGFP-tagged) - Mouse ring finger protein 139 (Rnf139) |
CNY 6,370.00 |
|
| MR209908L3 | Lenti ORF clone of Rnf139 (Myc-DDK-tagged) - Mouse ring finger protein 139 (Rnf139) |
CNY 7,700.00 |
|
| MR209908L4 | Lenti ORF clone of Rnf139 (mGFP-tagged) - Mouse ring finger protein 139 (Rnf139) |
CNY 7,700.00 |
