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Ddr1 (BC065998) Mouse Tagged ORF Clone
CAT＃: MR210041
- TrueORF®
Ddr1 (Myc-DDK-tagged) - Mouse discoidin domain receptor family, member 1 (cDNA clone MGC:90037 IMAGE:5686085)
ORF Plasmid: tGFP
"BC065998" in other vectors (4)
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CNY 5,024.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 6030432F18, CD167a, PTK3A |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210041 representing BC065998
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGACAGGGACCCTCTCATCTCTACTGCTGCTGCTACTCTTGGTGACAATTGGAGATGCTGACATGA AGGGACATTTTGACCCTGCCAAGTGCCGCTATGCCCTGGGCATGCAGGACCGCACCATTCCTGACAGCGA TATCTCTGTGTCCAGCTCCTGGTCGGACTCTACCGCTGCCCGCCACAGCAGGCTGGAAAGCAGTGATGGA GATGGGGCTTGGTGCCCTGCAGGGCCTGTGTTCCCCAAAGAAGAGGAGTACTTGCAGGTGGACCTTCGTA GGCTACACCTGGTGGCTCTGGTGGGCACCCAGGGCCGCCATGCTGGGGGTCTGGGCAAAGAGTTCTCCCG AAGCTATCGGTTGCGTTACTCCCGAGATGGCCGCCGCTGGATGGACTGGAAGGACCGCTGGGGACAGGAG GTGATTTCGGGTAACGAGGATCCCGGGGGAGTAGTGCTGAAGGACCTTGGGCCCCCCATGGTGGCCCGGC TGGTCCGCTTCTACCCCAGGGCTGACCGGGTCATGAGTGTCTGTCTTCGGGTGGAGCTCTATGGCTGCCT CTGGCGGGATGGACTCCTGTCATATACAGCCCCCGTGGGGCAGACCATGCAGTTATCTGAGGTGATGGTA CATCTCAATGATTCCACTTACGATGGATATACTGCTGGAGGGCTGCAGTATGGCGGTCTGGGCCAGCTGG CAGATGGCGTGGTGGGCCTGGATGATTTCAGGCAGAGCCAGGAGCTGCGGGGCTGGCCAGGCTATGACTA TGTGGGATGGAGCAATCAGAGCTTCCCCACGGGCTACGTGGAGATGGAGTTTGAGTTTGATCGGTTGAGG ACCTTCCAGACCATGCAGGTCCACTGTAACAACATGCACACTCTGGGAGCCCGCCTACCAGGCGGGGTGG AATGCCGGTTTAAAAGGGGTCCCGCCATGGCCTGGGAAGGAGAGCCTGTCCGCCATGCTCTGGGAGGCAG CCTTGGAGACCCCAGAGCCCGGGCCATCTCAGTGCCCCTGGGTGGCCACGTGGGCCGCTTTCTGCAGTGC AGATTCCTCTTTGCAGGTCCTTGGTTACTCTTCAGTGAGATCTCTTTCATCTCAGATGTGGTGAACGACT CCTCTGACACCTTCCCACCAGCCCCCTGGTGGCCACCTGGCCCGCCTCCCACCAACTTCAGCAGCTTGGA GCTGGAGCCCCGGGGTCAACAGCCAGTGGCCAAGGCGGAGGGGAGCCCAACTGCCATCCTCATTGGCTGC CTGGTGGCCATCATCCTGCTGCTGCTTCTCATCATCGCGCTGATGCTCTGGAGGCTGCACTGGCGCCGGC TGCTCAGCAAGGCTGAGCGCCGCGTGTTGGAGGAGGAGCTGACGGTTCACCTTTCTGTCCCTGGGGACAC CATCCTCATCAACAACCGCCCAGGACCCCGAGAGCCACCCCCTTACCAGGAGCCCCGGCCTCGGGGGACT CCACCCCATTCTGCACCCTGCGTCCCCAACGGCTCTGCCTGCAGTGGGGACTATATGGAGCCCGAGAAGC CGGGTGCCCCGCTTCTACCCCCACCTCCCCAGAACAGCGTCCCCCATTATGCCGAAGCTGACATTGTCAC CCTGCAGGGCGTCACTGGGGGCAACACCTACGCTGTGCCTGCACTGCCCCCAGGGGCGGTTGGGGATGGG CCCCCCAGAGTGGATTTCCCTCGGTCACGGCTCCGCTTCAAGGAGAAGCTTGGCGAGGGCCAATTTGGGG AGGTACACCTGTGTGAAGTAGAGGACCCGGCAGCCCTTTCCCAATGGGAGCCATCCAGGGGAAGCTGGAC TCGAAAACCAGAGAAGCCTATGGCACCGCACCCCACCGTCTCCATACTTGCCCATTCCCAAGAGGCAGTG TCTCTGCGGGTGGGCTGGGCCTGCCCGAGGAGCAGATACACACACTCCCTTCCGTTTCTTCCTTCCATCT GCCAAGTGCCACCCTCCCAGCTGGCCCTGTGGTTGGGATCCTGCCTGACTTCTTCCAGCTTTCTTCTTGT CACACACTGGACGCCGCCAGCTGAGAAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210041 representing BC065998
Red=Cloning site Green=Tags(s) MGTGTLSSLLLLLLLVTIGDADMKGHFDPAKCRYALGMQDRTIPDSDISVSSSWSDSTAARHSRLESSDG DGAWCPAGPVFPKEEEYLQVDLRRLHLVALVGTQGRHAGGLGKEFSRSYRLRYSRDGRRWMDWKDRWGQE VISGNEDPGGVVLKDLGPPMVARLVRFYPRADRVMSVCLRVELYGCLWRDGLLSYTAPVGQTMQLSEVMV HLNDSTYDGYTAGGLQYGGLGQLADGVVGLDDFRQSQELRGWPGYDYVGWSNQSFPTGYVEMEFEFDRLR TFQTMQVHCNNMHTLGARLPGGVECRFKRGPAMAWEGEPVRHALGGSLGDPRARAISVPLGGHVGRFLQC RFLFAGPWLLFSEISFISDVVNDSSDTFPPAPWWPPGPPPTNFSSLELEPRGQQPVAKAEGSPTAILIGC LVAIILLLLLIIALMLWRLHWRRLLSKAERRVLEEELTVHLSVPGDTILINNRPGPREPPPYQEPRPRGT PPHSAPCVPNGSACSGDYMEPEKPGAPLLPPPPQNSVPHYAEADIVTLQGVTGGNTYAVPALPPGAVGDG PPRVDFPRSRLRFKEKLGEGQFGEVHLCEVEDPAALSQWEPSRGSWTRKPEKPMAPHPTVSILAHSQEAV SLRVGWACPRSRYTHSLPFLPSICQVPPSQLALWLGSCLTSSSFLLVTHWTPPAEN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC065998 |
| ORF Size | 2058 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC065998, AAH65998 |
| RefSeq Size | 2582 bp |
| RefSeq ORF | 2060 bp |
| Locus ID | 12305 |
| MW | 94.6 kDa |
| Gene Summary | Tyrosine kinase that functions as cell surface receptor for fibrillar collagen and regulates cell attachment to the extracellular matrix, remodeling of the extracellular matrix, cell migration, differentiation, survival and cell proliferation. Collagen binding triggers a signaling pathway that involves SRC and leads to the activation of MAP kinases. Regulates remodeling of the extracellular matrix by up-regulation of the matrix metalloproteinases MMP2, MMP7 and MMP9, and thereby facilitates cell migration and wound healing, but also tumor cell invasion. Promotes smooth muscle cell migration, and thereby contributes to arterial wound healing. Phosphorylates PTPN11 (By similarity). Required for normal blastocyst implantation during pregnancy, for normal mammary gland differentiation and normal lactation. Required for normal ear morphology and normal hearing.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220686 | Ddr1 (untagged) - Mouse discoidin domain receptor family, member 1 (cDNA clone MGC:90037 IMAGE:5686085), (10ug) |
CNY 9,120.00 |
|
| MG210041 | Ddr1 (tGFP-tagged) - Mouse discoidin domain receptor family, member 1 (cDNA clone MGC:90037 IMAGE:5686085) |
CNY 4,280.00 |
|
| MR210041L3 | Lenti ORF clone of Ddr1 (Myc-DDK-tagged) - Mouse discoidin domain receptor family, member 1 (cDNA clone MGC:90037 IMAGE:5686085) |
CNY 5,800.00 |
|
| MR210041L4 | Lenti ORF clone of Ddr1 (mGFP-tagged) - Mouse discoidin domain receptor family, member 1 (cDNA clone MGC:90037 IMAGE:5686085) |
CNY 5,800.00 |
