Hnrpm (BC065172) Mouse Tagged ORF Clone

CAT#: MR210063

  • TrueORF®

Hnrpm (Myc-DDK-tagged) - Mouse heterogeneous nuclear ribonucleoprotein M (cDNA clone MGC:90027 IMAGE:6415170)

ORF Plasmid: DDK tGFP



  "BC065172" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 6,840.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms 2610023M21Rik; AA409009; Hnrpm; mKIAA4193
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR210063 representing BC065172
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCAGGGGTCGAAGCGGCAGCCGAAGTGGCGGCGACAGAACCCAAAATGGAGGAAGAGAGCGGCG
CGCCCTGCGTGCCGAGCGGCAACGGAGCTCCGGGCCCGAAGGGTGAAGAACGACCTACTCAGAATGAGAA
GAGGAAGGAGAAAAACATAAAAAGAGGAGGCAATCGCTTTGAGCCATATTCCAACCCAACTAAAAGATAC
AGAGCCTTCATTACAAATATACCTTTTGATGTGAAATGGCAGTCACTTAAAGACCTGGTTAAAGAAAAAG
TTGGTGAGGTAACATACGTGGAGCTCTTAATGGACGCTGAAGGAAAGTCAAGGGGATGTGCTGTTGTTGA
ATTCAAGATGGAGGAGAGCATGAAAAAAGCTGCTGAAGTTCTAAACAAGCATAGTCTGAGTGGAAGGCCA
CTGAAAGTCAAGGAAGATCCTGATGGTGAACATGCAAGGAGAGCAATGCAAAAGGCTGGAAGACTTGGAA
GCACAGTATTTGTAGCAAATCTGGATTATAAAGTTGGCTGGAAGAAACTGAAGGAAGTATTTAGTATGGC
TGGTGTGGTGGTCCGAGCAGACATTCTGGAAGATAAAGATGGGAAAAGTCGTGGAATAGGCATTGTGACT
TTTGAACAGTCCATTGAAGCTGTGCAAGCAATATCTATGTTTAATGGCCAGTTGCTGTTTGATAGACCGA
TGCACGTCAAGATGGATGAGAGGGCTTTACCAAAGGGAGACTTTTTTCCTCCTGAACGCCCACAGCAACT
TCCCCATGGACTTGGTGGTATTGGCATGGGATTAGGACCTGGAGGTCAGCCTATTGATGCCAACCATCTG
AGCAAAGGCATTGGAATGGGAAACCTAGGACCTGCAGGGATGGGAATGGAAGGCATAGGATTTGGAATAA
ATAAAATTGGAGGCATGGAAGGACCCTTTGGTGGTGGTATGGAAAACATGGGCCGATTTGGATCCGGGAT
GAACATGGGCCGAATAAATGAAATCCTAAGTAATGCACTGAAGAGAGGAGAGATCATTGCAAAACAGGGA
GGAGGTGGAGCTGGAGGCAGTGTCCCTGGGATCGAGAGGATGGGCCCTGGCATTGACCGCATTAGCGGTG
CTGGCATGGAGCGCATGGGCGCAGGCTTAGGCCATGGCATGGATCGAGTGGGCTCTGAGATTGAGCGCAT
GGGCCTGGTCATGGACCGCATGGGCTCAGTTGAGCGCATGGGCTCCAGCATTGAGCGCATGGGCCCACTA
GGCCTTGACCACATGGCCTCCAGTATTGAGCGCATGGGCCAGACCATGGAGCGCATTGGTTCTGGCGTGG
AGCGCATGGGTGCCGGCATGGGCTTCGGCCTGGAGCGCATGGCCGCACCCATTGACCGTGTGGGCCAAAC
CATTGAGCGCATGGGCTCTGGTGTAGAGCGCATGGGTCCTGCCATTGAACGCATGGGCCTAAGCATGGAT
CGGATGGTGCCCACAGGCATGGGGGCCAGCCTGGAGCGCATGGGTCCTGTGATGGATCGGATGGCCACCG
GCCTGGAGCGCATGGGCGCCAACAACCTGGAGCGCATGGGCCTGGAGCGTATGGGAGCCAACAGTCTTGA
GCGCATGGGCCTGGAGCGAATGGGCGCCAACAGCCTGGAACGTATGGGCCCTGCCATGGGCCCAGCATTG
GGCGCTGGTATTGAGCGAATGGGCCTGGCCATGGGTGGAGCCGGAGGTGCTAGCTTTGACCGAGCCATTG
AGATGGAGCGGGGCAACTTTGGAGGAAGCTTCGCAGGTTCCTTTGGCGGAGCTGGAGGCCATGCACCTGG
AGTAGCCAGGAAGGCCTGCCAGATATTTGTGAGAAATCTCCCATTTGATTTTACATGGAAGATGCTAAAG
GACAAGTTCAATGAATGTGGCCACGTGCTGTACGCCGACATCAAGATGGAGAACGGAAAGTCCAAGGGGT
GCGGTGTGGTTAAGTTTGAGTCTCCAGAGGTGGCTGAGAGAGCCTGCCGGATGATGAATGGCATGAAGCT
GAGTGGCCGAGAGATTGATGTTCGAATTGATAGAAATGCT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR210063 representing BC065172
Red=Cloning site Green=Tags(s)

MAAGVEAAAEVAATEPKMEEESGAPCVPSGNGAPGPKGEERPTQNEKRKEKNIKRGGNRFEPYSNPTKRY
RAFITNIPFDVKWQSLKDLVKEKVGEVTYVELLMDAEGKSRGCAVVEFKMEESMKKAAEVLNKHSLSGRP
LKVKEDPDGEHARRAMQKAGRLGSTVFVANLDYKVGWKKLKEVFSMAGVVVRADILEDKDGKSRGIGIVT
FEQSIEAVQAISMFNGQLLFDRPMHVKMDERALPKGDFFPPERPQQLPHGLGGIGMGLGPGGQPIDANHL
SKGIGMGNLGPAGMGMEGIGFGINKIGGMEGPFGGGMENMGRFGSGMNMGRINEILSNALKRGEIIAKQG
GGGAGGSVPGIERMGPGIDRISGAGMERMGAGLGHGMDRVGSEIERMGLVMDRMGSVERMGSSIERMGPL
GLDHMASSIERMGQTMERIGSGVERMGAGMGFGLERMAAPIDRVGQTIERMGSGVERMGPAIERMGLSMD
RMVPTGMGASLERMGPVMDRMATGLERMGANNLERMGLERMGANSLERMGLERMGANSLERMGPAMGPAL
GAGIERMGLAMGGAGGASFDRAIEMERGNFGGSFAGSFGGAGGHAPGVARKACQIFVRNLPFDFTWKMLK
DKFNECGHVLYADIKMENGKSKGCGVVKFESPEVAERACRMMNGMKLSGREIDVRIDRNA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC065172
ORF Size 2070 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC065172.1
RefSeq Size 2362 bp
RefSeq ORF 2072 bp
Locus ID 76936
MW 86.6 kDa
Gene Summary Pre-mRNA binding protein in vivo, binds avidly to poly(G) and poly(U) RNA homopolymers in vitro. Involved in splicing. Acts as a receptor for carcinoembryonic antigen in Kupffer cells, may initiate a series of signaling events leading to tyrosine phosphorylation of proteins and induction of IL-1 alpha, IL-6, IL-10 and tumor necrosis factor alpha cytokines (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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