Crtc2 (NM_028881) Mouse Tagged ORF Clone
CAT#: MR210074
- TrueORF®
Crtc2 (Myc-DDK-tagged) - Mouse CREB regulated transcription coactivator 2 (Crtc2)
ORF Plasmid: tGFP
"NM_028881" in other vectors (4)
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CNY 5,080.00
CNY 6,080.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 4632407F12Rik; mTORC2; Torc2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR210074 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGACGTCAGGGGCGAACGGGCCGGGTTCCGCCACAGCCTCAGCGTCCAATCCACGCAAGTTTAGTG AGAAGATTGCGCTGCAGAAGCAGCGTCAGGCCGAGGAGACGGCGGCCTTCGAGGAGGTGATGATGGACAT CGGCTCCACACGGTTACAGGCCCAAAAACTGCGACTGGCTTATACAAGGAGCTCCCATTATGGTGGTTCT CTGCCCAATGTTAACCAGATTGGCTGTGGCCTGGCTGAGTTCCAGAGCCCCCTTCACTCACCTTTGGATT CATCTCGGAGCACTCGGCACCATGGGTTGGTGGAACGGGTACAACGAGATGCCCGCAGAATGGTGTCCCC ACTCCGCCGATACCCCCGCCACATTGACAGTTCTCCATATAGCCCTGCCTATTTATCTCCTCCCCCAGAG TCTGGCTGGCGAAGGATGATGCCCTGGGGCAACCTCCCAGCTGAGAAGGGGCAGTTGTTTAGACTGCCGT CTGCACTTAACAGGACAAGCTCTGACTCTGCTCTTCACACAAGTGTGATGAACCCTAACCCCCAAGACAC TTATCCAGGCCCCACACCTCCCAGTGTCCTGCCCAGCCGCCGAGGAGGAGGTTTTCTGGATGGTGAGATG GATGCTAAAGTCCCTGCTATTGAGGAGAACGTGGTAGATGACAAGCATCTGCTGAAACCTTGGGATGCTA AGAAGCTGTCCTCATCTTCCTCTCGACCTCGATCCTGTGAAGTCCCTGGAATCAACATCTTTCCATCTCC TGACCAGCCTGCTAATGTGCCTGTCCTCCCACCTGCCATGAACACAGGAGGCTCCCTACCTGACCTCACC AACCTACACTTCCCTCCTCCACTGCCCACACCCCTGGACCCTGAGGAGACAGTCTATCCCAGCCTGAGTG GGGGCAACAGTACCACCAATTTGACCCATACTATGACCCATTTGGGCATCAGTGGAGGTCTGGGCTTGGG CCCCAGCTATGATGTACCAGGACTTCACTCACCTCTTAGCCACCCATCCCTGCAGTCCTCCCTAAGCAAT CCCAACCTCCAGGCTTCCCTAAGTAGTCCTCAACCCCAGCTCCAGGGCTCCCATAGTCACCCATCACTGC CTGCCTCCTCCTTGGCCCACCATGCACTGCCCACCACCTCCCTGGGCCACCCGTCACTTAGTGCCCCAGC CCTTTCCTCCTCCTCTTCCTCTTCGTCCACTTCATCTCCTGTCCTGAGTGCTCCCCCTTACCCAGCTTCT ACTCCTGGGGCCTCTCCCCGCCACCGTCGTGTGCCCCTCAGCCCCCTGAGCTTGCCCGCGGGCCCAGCCG ACGCCAGAAGGTCCCAACAGCAGCTGCCCAAACAGTTTTCGCCAACAATGTCACCCACCTTGTCTTCCAT CACTCAGGGCGTTCCCTTGGATACCAGTAAATTGCCTACTGACCAGCGATTGCCCCCATACCCATATAGC CCCCCAAGTCTAGTTATACCCACCCATCCACCCACCCCAAAGTCTCTACAGCAGTTGCCCTCTCAGGCCT GCTTAGTGCAGCCCTCAGGTGGACAGCCTCCAGGCAGGCAGCCACATTATGGGGCACTGTACCCACCTGG GTCCAGTGGACATGGGCAACAGCCTTACCACCGCCCAATAAATGACTTCAGCTTGGGGAATCTGGAGCAA TTCAACATGGAGAGCCCATCAACCAGCCTGGTGCTGGATCCCCCTGCCTTTTCTGAAGGTCCTGGATTTT TAGGGAGTGAGGGATCAATGAGTGGCCCCCAGGACCCTCACGTCCTCAACCACCAGAACTTGACCCACTG TTCCCGCCATGGCTCAGGACCTAATATCATACTCACAGGAGACTCTTCCCCAGGTTTCTCTAAGGAGATT GCAGCAGCCCTGGCTGGAGTGCCTGGCTTTGAGGTGTCAGCATCTGGGTTGGAGCTGGGCCTGGGGCTAG AAGATGAGCTGCGCATGGAGCCACTGGGCCTGGAAGGACTAACTATGCTGAGTGACCCCTGTGCCCTGCT GCCTGACCCTGCTGTGGAGGACTCATTCCGTAGTGATCGGCTACAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210074 protein sequence
Red=Cloning site Green=Tags(s) MATSGANGPGSATASASNPRKFSEKIALQKQRQAEETAAFEEVMMDIGSTRLQAQKLRLAYTRSSHYGGS LPNVNQIGCGLAEFQSPLHSPLDSSRSTRHHGLVERVQRDARRMVSPLRRYPRHIDSSPYSPAYLSPPPE SGWRRMMPWGNLPAEKGQLFRLPSALNRTSSDSALHTSVMNPNPQDTYPGPTPPSVLPSRRGGGFLDGEM DAKVPAIEENVVDDKHLLKPWDAKKLSSSSSRPRSCEVPGINIFPSPDQPANVPVLPPAMNTGGSLPDLT NLHFPPPLPTPLDPEETVYPSLSGGNSTTNLTHTMTHLGISGGLGLGPSYDVPGLHSPLSHPSLQSSLSN PNLQASLSSPQPQLQGSHSHPSLPASSLAHHALPTTSLGHPSLSAPALSSSSSSSSTSSPVLSAPPYPAS TPGASPRHRRVPLSPLSLPAGPADARRSQQQLPKQFSPTMSPTLSSITQGVPLDTSKLPTDQRLPPYPYS PPSLVIPTHPPTPKSLQQLPSQACLVQPSGGQPPGRQPHYGALYPPGSSGHGQQPYHRPINDFSLGNLEQ FNMESPSTSLVLDPPAFSEGPGFLGSEGSMSGPQDPHVLNHQNLTHCSRHGSGPNIILTGDSSPGFSKEI AAALAGVPGFEVSASGLELGLGLEDELRMEPLGLEGLTMLSDPCALLPDPAVEDSFRSDRLQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_028881 |
ORF Size | 2079 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_028881.3 |
RefSeq Size | 2647 bp |
RefSeq ORF | 2079 bp |
Locus ID | 74343 |
UniProt ID | Q3U182 |
MW | 73.2 kDa |
Gene Summary | Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites (PubMed:29211348). Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated (PubMed:29211348). Acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates gluconeogenesis as a component of the LKB1/AMPK/TORC2 signaling pathway. Regulates the expression of specific genes such as the steroidogenic gene, StAR. Potent coactivator of PPARGC1A and inducer of mitochondrial biogenesis in muscle cells (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC204255 | Crtc2 (untagged) - Mouse CREB regulated transcription coactivator 2 (Crtc2), (10ug) |
CNY 5,080.00 |
|
MG210074 | Crtc2 (tGFP-tagged) - Mouse CREB regulated transcription coactivator 2 (Crtc2) |
CNY 6,650.00 |
|
MR210074L3 | Lenti ORF clone of Crtc2 (Myc-DDK-tagged) - Mouse CREB regulated transcription coactivator 2 (Crtc2) |
CNY 7,980.00 |
|
MR210074L4 | Lenti ORF clone of Crtc2 (mGFP-tagged) - Mouse CREB regulated transcription coactivator 2 (Crtc2) |
CNY 7,480.00 |