C1s (BC018319) Mouse Tagged ORF Clone

CAT#: MR210085

  • TrueORF®

C1s (Myc-DDK-tagged) - Mouse complement component 1, s subcomponent (cDNA clone MGC:19094 IMAGE:4196654)

ORF Plasmid: DDK tGFP



  "BC018319" in other vectors (3)

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Mouse Protein / Lysate Request

CNY 5,088.00

CNY 6,940.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms MGC37773, C1sa
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR210085 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCAAATCGCCAGGGATGTGGTGTTTGGTCCTGTTTTCTCTTTTGGCATCATTTTCTGCTGAGCCCA
CCATGCATGGGGAGATCCTGTCCCCTAACTATCCCCAGGCGTACCCCAATGACGTCGTGAAATCTTGGGA
CATTGAAGTCCCAGAGGGGTTTGGGATTCACCTCTACTTTACCCATGTGGACATAGAGCCGTCAGAGAGC
TGTGCATACGACTCAGTGCAGATAATCTCAGGAGGCATCGAGGAAGGGAGACTCTGTGGCCAGAAGACCA
GCAAGAGCCCCAACTCCCCCATTATAGAAGAGTTTCAGTTCCCATACAATAAACTTCAGGTGGTCTTTAC
CTCAGACTTCTCCAACGAAGAACGGTTTACGGGCTTTGCAGCATACTACACTGCCATAGACATAAATGAA
TGCACAGATTTTACAGATGTCCCCTGTAGCCACTTCTGCAATAACTTCATTGGTGGATACTTCTGCTCCT
GTCCCCCAGAATACTTCCTCCACGATGACATGAGGAATTGTGGAGTCAACTGTAGTGGAGATGTGTTCAC
TGCCCTGATTGGGGAGATCTCAAGTCCCAATTATCCCAATCCATACCCGGAGAACTCAAGGTGTGAATAC
CAGATTCAGCTGCAGGAGGGCTTCCAAGTGGTGGTGACGATGCAAAGAGAAGATTTTGATGTGGAGCCTG
CTGACTCAGAGGGGAACTGCCCTGACAGTTTAACTTTTGCTTCAAAAAACCAACAGTTTGGTCCTTACTG
TGGTAATGGGTTCCCTGGACCGCTAACTATTAGAACCCAGAGCAATACTCTCGGCATTGTCTTTCAAACT
GACCTAATGGGGCAAAAGAAGGGTTGGAAGCTTCGTTACCATGGAGATCCCATCTCCTGTGCCAAAAAAA
TCACTGCCAATTCTACTTGGGAGCCCGATAAGGCAAAATACGTGTTTAAAGATGTGGTGAAGATAACCTG
TGTGGACGGATTTGAAGTTGTGGAGGGACATGTTAGCTCTACTTCATACTATTCCACTTGTCAAAGCGAT
GGACAGTGGAGCAACTCCGGTCTAAAATGTCAACCTGTGTATTGTGGAATTCCAGATCCCATTGCGAATG
GTAAAGTTGAAGAGCCAGAGAATAGTGTGTTTGGTACTGTTGTTCACTACACGTGTGAAGAGCCATATTA
CTACATGGAACATGAAGAAGGTGGGGAGTATCGATGTGCTGCAAATGGGAGATGGGTAAATGACCAGCTG
GGTATTGAGCTTCCAAGATGTATTCCAGCCTGTGGAGTACCCACCGAGCCCTTTCAAGTGCATCAGAGGA
TATTTGGAGGACAACCTGCAAAGATTGAAAATTTTCCCTGGCAAGTCTTCTTTAATCACCCCAGAGCTAG
TGGGGCTCTTATTAATGAGTACTGGGTGCTGACGGCTGCTCACGTTTTGGAGAAAATCTCCGACCCTTTA
ATGTATGTCGGGACCATGTCTGTGAGGACAACTCTCTTGGAAAATGCCCAGAGACTCTACAGTAAACGTG
TCTTTATTCATCCCAGCTGGAAGAAAGAAGATGACCCAAACACACGGACAAATTTTGACAATGACATTGC
CCTGGTGCAGCTGAAAGACCCTGTGAAAATGGGACCCAAAGTCTCCCCCATCTGCCTACCAGGCACCTCC
TCAGAGTACAACGTCTCACCGGGTGACATGGGGCTGATCTCAGGGTGGGGCAGTACAGAAAAGAAAGTCT
TTGTTATCAACCTCAGAGGGGCAAAGGTACCAGTAACCTCTTTAGAAACCTGCAAACAGGTGAAAGAGGA
GAACCCCACTGTAAGGCCTGAGGACTATGTTTTCACTGACAACATGATCTGTGCTGGCGAGAAAGGTGTT
GACAGCTGTCACGGGGACAGCGGCGGGGCTTTTGCTTTCCAGGTCCCCAATGTCACGGTCCCCAAATTCT
ATGTAGCTGGCTTGGTGTCTTGGGGGAAAAGGTGTGGGACTTATGGGGTTTACACAAAGGTAAAGAATTA
CGTGGACTGGATCCTGAAAACTATGCAGGAGAATAGTGGGCCCAGGAAGGAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR210085 protein sequence
Red=Cloning site Green=Tags(s)

MGKSPGMWCLVLFSLLASFSAEPTMHGEILSPNYPQAYPNDVVKSWDIEVPEGFGIHLYFTHVDIEPSES
CAYDSVQIISGGIEEGRLCGQKTSKSPNSPIIEEFQFPYNKLQVVFTSDFSNEERFTGFAAYYTAIDINE
CTDFTDVPCSHFCNNFIGGYFCSCPPEYFLHDDMRNCGVNCSGDVFTALIGEISSPNYPNPYPENSRCEY
QIQLQEGFQVVVTMQREDFDVEPADSEGNCPDSLTFASKNQQFGPYCGNGFPGPLTIRTQSNTLGIVFQT
DLMGQKKGWKLRYHGDPISCAKKITANSTWEPDKAKYVFKDVVKITCVDGFEVVEGHVSSTSYYSTCQSD
GQWSNSGLKCQPVYCGIPDPIANGKVEEPENSVFGTVVHYTCEEPYYYMEHEEGGEYRCAANGRWVNDQL
GIELPRCIPACGVPTEPFQVHQRIFGGQPAKIENFPWQVFFNHPRASGALINEYWVLTAAHVLEKISDPL
MYVGTMSVRTTLLENAQRLYSKRVFIHPSWKKEDDPNTRTNFDNDIALVQLKDPVKMGPKVSPICLPGTS
SEYNVSPGDMGLISGWGSTEKKVFVINLRGAKVPVTSLETCKQVKEENPTVRPEDYVFTDNMICAGEKGV
DSCHGDSGGAFAFQVPNVTVPKFYVAGLVSWGKRCGTYGVYTKVKNYVDWILKTMQENSGPRKD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC018319
ORF Size 2082 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC018319.1
RefSeq Size 2855 bp
RefSeq ORF 2084 bp
Locus ID 50908
MW 77.4 kDa
Gene Summary C1s B chain is a serine protease that combines with C1q and C1r to form C1, the first component of the classical pathway of the complement system. C1r activates C1s so that it can, in turn, activate C2 and C4 (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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