United States
Germany
Japan
United Kingdom
China
Mtrr (NM_172480) Mouse Tagged ORF Clone
CAT＃: MR210099
- TrueORF®
Mtrr (Myc-DDK-tagged) - Mouse 5-methyltetrahydrofolate-homocysteine methyltransferase reductase (Mtrr)
ORF Plasmid: tGFP
"NM_172480" in other vectors (3)
Need custom modification / cloning service?
Get a free quote
CNY 5,104.00
CNY 5,800.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 4732420G08; MSR |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210099 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGAAGGTTTTTGTTACTTTATGCTACACAGCGAGGACAGGCAAAGGCCATAGCAGAAGAAATAAGTG AGCAGGCTGTGTCACACGGGTTTTCCGCAGATCTTCACTGTATCAGTGAGTCGGAAAAGTATGATCTGAA AACAGAAACTGGCCCTCTGGTGATGGTTGTATCTACCACGGGTACAGGAGACCCACCTGACACAGCCCGG AAGTTTGTTAAAGAAATACACAACAAGACACTCCCCACGGACTATTTTGCTCACCTGCGGTATGGATTAT TGGGCTTGGGTGACTCAGAATACACATACTTCTGCAATGGAGGGAAAGTAATTGATAAACGACTTCAGGA GCTTGGAGCCCAGCGTTTCTACGACACCGGACATGCCGATGACTGTGTCGGTTTAGAGCTTGTGGTAGAG CCGTGGATTGATGGACTCTGGGCTGCTCTCACAAAGCACTTTAAGTCACTTGGAGGACAAGAAAACATGA GTGACACACTCTCGCGAGCATCAGATGCCCCCTTGAGCACTGCCATGAAGCCGGAGTTGTTGCACATCCA GTCACAATTTGAACTGCTGAGATTAGAGGACGTGGGGGAGAGGGATTCTGAGCTTCGGGAACAAAATGAG ACAAACAGGGGTCAGCAGGGTCGGATCGAAGACTTTGACTCCTCGCTTGTGCACTCGGTCCCCCCACTCT CCCAGAGCTCTCTAAGTATTCCTGCTGTGCCCCCAGAGTATTTGGAGGTCCATCTTCAAGAGTCTCTTGG CCAGGAGGAAAACCAAGCATCTGTGCCTTCAGGGGATCCAAGTTTTCAAGTTCCAATTTCAAAAGCCATT CGTCTGACTACAAATGATGCCGTAAAGAGCACTCTGCTGTTGGAACTGGACATTTCAAAAATAGAGTTTT CCCATCAACCTGGAGACTCCTTCAATGTGACCTGTCCAAACAGTGATCGTGAGGTAGAAGAACTGCTCCA AAGGCTGCAGCTGGCTGATAAACGAGCGCACCGTGTCATCTTGAAAATTAAGACGGACACTAAGAAGAAA GGAGCTGCCCTGCCTGCGCATGTGCCTGAGGGGCGTTCTCTCCAGTTCATCCTCACCTGGTGTCTTGAAA TACGAGCAGTTCCTAAAAAGGCCTTTTTGCGGGCCCTTGCGGAGCACACCAGCAGTGCCACTGAAAAGCG GAGGCTGCAGGAGCTGTGCAGTAAACAAGGAGCCGCTGATTACAACCGCTTTATCCGAGATGCCAGCGTC TGCCTGCTGGACCTCCTGCTCACCTTCCCGTCCTGCCAGCCTCCGCTCAGCCTCCTGCTGGAGCACCTTC CTAAACTTCAGCCTCGACCGTACTCATGTGCAAGCTCCAGCTTACGCCACCCAGACAAGCTTCACTTCGT GTTTAACATCGTGGAGTTCCCACCGAGCACCACTGCAGCCTCCCCGCGGAAGGGAGTGTGCACGGGCTGG CTAGCCACATTGGTTGCTCCATTCCTTCAGCCAAACACAGACGTTTCGAATGCAGACAGTGGGGACACTC TGGCTCCTGAGATACGTATCTCTCCTCGGGCAACAAATGCTTTCCACTTACCAGAGGACCCGTCGGCCCC CATCATAATGGTGGGTCCAGGAACTGGCGTAGCCCCCTTTGTTGGCTTCCTCCAGCACAGAGAAAAACTT CAAGAACAACACCCAGATGGGAAATTTGGAGCAATGTGGCTGTTCTTTGGCTGCAGACACAAGGACAGAG ACTACTTGTTCAGGGAGGAGCTCAGGCATTTCCTCAAGACTGGGGTCTTGACTCATCTGAAGGTCTCGTT TTCAAGAGACGCTGCCCCTGACGGGGAGGAGGCCCCAGCAAAGTATGTGCAAGACAACCTCCAGCGGCAC AGCCAGCAGGTGGCCAGGACCCTCCTTCAGGAGAATGGCTACATTTACGTGTGTGGAGACGCCAAGAATA TGGCCAAGGACGTGAACGACACCCTCATAGGAATCATAAGCAATGAAGCTGGTGTTGACAAACTAGAAGC AATGAAGACGCTGGCAACGTTAAAGCAAGAGAAACGATATCTGCAGGACATCTGGTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210099 protein sequence
Red=Cloning site Green=Tags(s) MRRFLLLYATQRGQAKAIAEEISEQAVSHGFSADLHCISESEKYDLKTETGPLVMVVSTTGTGDPPDTAR KFVKEIHNKTLPTDYFAHLRYGLLGLGDSEYTYFCNGGKVIDKRLQELGAQRFYDTGHADDCVGLELVVE PWIDGLWAALTKHFKSLGGQENMSDTLSRASDAPLSTAMKPELLHIQSQFELLRLEDVGERDSELREQNE TNRGQQGRIEDFDSSLVHSVPPLSQSSLSIPAVPPEYLEVHLQESLGQEENQASVPSGDPSFQVPISKAI RLTTNDAVKSTLLLELDISKIEFSHQPGDSFNVTCPNSDREVEELLQRLQLADKRAHRVILKIKTDTKKK GAALPAHVPEGRSLQFILTWCLEIRAVPKKAFLRALAEHTSSATEKRRLQELCSKQGAADYNRFIRDASV CLLDLLLTFPSCQPPLSLLLEHLPKLQPRPYSCASSSLRHPDKLHFVFNIVEFPPSTTAASPRKGVCTGW LATLVAPFLQPNTDVSNADSGDTLAPEIRISPRATNAFHLPEDPSAPIIMVGPGTGVAPFVGFLQHREKL QEQHPDGKFGAMWLFFGCRHKDRDYLFREELRHFLKTGVLTHLKVSFSRDAAPDGEEAPAKYVQDNLQRH SQQVARTLLQENGYIYVCGDAKNMAKDVNDTLIGIISNEAGVDKLEAMKTLATLKQEKRYLQDIWS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_172480 |
| ORF Size | 2091 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq Size | 3692 bp |
| RefSeq ORF | 2091 bp |
| Locus ID | 210009 |
| UniProt ID | Q8C1A3 |
| MW | 77.6 kDa |
| Gene Summary | Methionine is an essential amino acid required for protein synthesis and one-carbon metabolism. Its synthesis is catalyzed by the enzyme methionine synthase. Methionine synthase eventually becomes inactive due to the oxidation of its cob(I)alamin cofactor. The protein encoded by this gene regenerates a functional methionine synthase via reductive methylation. It is a member of the ferredoxin-NADP(+) reductase (FNR) family of electron transferases. Mutations in a similar gene in human have been associated with cblE complementation type homocystinuria-megaloblastic anemia and susceptibility to folate-sensitive neural tube defects. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG210099 | Mtrr (tGFP-tagged) - Mouse 5-methyltetrahydrofolate-homocysteine methyltransferase reductase (Mtrr) |
CNY 6,370.00 |
|
| MR210099L3 | Lenti ORF clone of Mtrr (Myc-DDK-tagged) - Mouse 5-methyltetrahydrofolate-homocysteine methyltransferase reductase (Mtrr) |
CNY 7,700.00 |
|
| MR210099L4 | Lenti ORF clone of Mtrr (mGFP-tagged) - Mouse 5-methyltetrahydrofolate-homocysteine methyltransferase reductase (Mtrr) |
CNY 7,700.00 |
