Erbb2 (BC046811) Mouse Tagged ORF Clone

CAT#: MR210207

  • TrueORF®

Erbb2 (Myc-DDK-tagged) - Mouse v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)

ORF Plasmid: DDK tGFP



  "BC046811" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 5,208.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit polyclonal Phospho-Mouse ERBB2(Y1140) Antibody
    • 400 ul

CNY 6,446.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms Neu, HER-2, c-erbB2, HER2, c-neu, mKIAA3023
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR210207 representing BC046811
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCTGGCGGCCTGGTGCCGTTGGGGGTTCCTCCTCGCCCTCCTGTCCCCCGGAGCCGCGGGTACCC
AAGTGTGTACCGGTACCGACATGAAGTTGCGACTCCCTGCCAGTCCTGAGACCCACCTGGACATGCTTCG
CCACCTCTACCAGGGCTGTCAGGTGGTGCAGGGCAATTTGGAGCTTACCTACCTGCCCGCCAATGCCAGC
CTCTCATTCCTGCAGGACATCCAGGAAGTCCAGGGATACATGCTCATCGCTCACAACCGAGTGAAACACG
TCCCACTGCAGAGGTTGCGCATCGTGAGAGGGACTCAGCTCTTTGAGGACAAGTATGCCCTGGCTGTGCT
AGACAACCGAGACCCTTTGGACAACGTCACCACCGCCGCCCCAGGCAGAACCCCAGAAGGGCTGCGGGAG
CTGCAGCTTCGAAGTCTCACAGAGATCTTGAAGGGAGGAGTTTTGATCCGTGGGAACCCTCAGCTCTGCT
ACCAGGACATGGTTTTGTGGAAGGATGTCCTCCGTAAGAATAACCAGCTGGCTCCTGTCGACATGGACAC
CAATCGTTCCCGGGCCTGTCCACCTTGTGCCCCAACCTGCAAAGACAATCACTGTTGGGGTGAGAGTCCT
GAAGACTGTCAGATCTTGACTGGCACCATCTGTACTAGTGGCTGTGCCCGGTGCAAGGGCCGGCTGCCCA
CTGACTGTTGCCATGAGCAGTGTGCTGCAGGCTGCACGGGTCCCAAGCATTCTGACTGCCTGGCCTGCCT
CCACTTCAATCATAGTGGTATCTGTGAGCTGCACTGCCCGGCCCTCATCACCTACAACACAGACACCTTC
GAGTCCATGCTCAACCCTGAGGGTCGCTACACCTTTGGTGCCAGCTGTGTGACCACCTGCCCCTACAACT
ACCTCTCCACGGAAGTGGGATCCTGCACTCTGGTCTGTCCCCCGAACAACCAAGAGGTCACAGCTGAGGA
CGGAACACAGCGGTGTGAGAAATGCAGCAAGCCCTGTGCTGGAGTATGCTATGGTCTGGGCATGGAGCAC
CTCCGAGGGGCGAGGGCCATCACCAGTGACAATATCCAGGAGTTTGCTGGCTGCAAGAAGATCTTTGGGA
GCCTGGCATTTTTGCCGGAGAGCTTTGATGGGAACCCCTCCTCCGGCGTTGCCCCACTGAAGCCAGAGCA
TCTCCAAGTGTTCGAAACCCTGGAGGAGATCACAGGTTACCTATACATTTCAGCATGGCCAGAGAGCTTC
CAAGACCTCAGTGTCTTCCAGAACCTTCGGGTCATTCGGGGACGGATTCTCCATGATGGTGCTTACTCAT
TGACGTTGCAAGGCCTGGGGATTCACTCACTGGGGCTACGCTCACTGCGGGAGCTGGGCAGTGGATTGGC
TCTCATTCACCGCAACACCCATCTCTGCTTTGTAAACACTGTACCTTGGGACCAGCTCTTCCGGAACCCG
CACCAGGCCCTACTCCACAGTGGGAACCGGCCAGAAGAGGCATGTGGTCTTGAGGGCTTGGTCTGTAACT
CACTGTGTGCCCGTGGGCACTGCTGGGGGCCAGGGCCCACCCAGTGTGTCAACTGCAGTCAGTTCCTCCG
GGGCCAGGAGTGTGTGGAGGAGTGCCGAGTATGGAAGGGGCTCCCCAGGGAGTATGTGAGGGGCAAGCAC
TGTCTGCCATGCCACCCCGAGTGTCAGCCTCAAAACAGCTCGGAGACCTGCTATGGATCGGAGGCTGACC
AGTGTGAGGCTTGTGCCCACTACAAGGACTCATCTTCCTGTGTGGCTCGCTGCCCCAGTGGTGTGAAGCC
AGACCTCTCCTACATGCCTATCTGGAAGTACCCGGATGAGGAGGGCATATGTCAGCCATGCCCCATCAAC
TGCACCCACTCATGTGTGGACCTGGACGAACGAGGCTGCCCAGCAGAGCAGAGAGCCAGCCCAGTGACAT
TCATCATTGCAACTGTGGTGGGCGTCCTGTTGTTCCTGATCATAGTGGTGGTCATTGGAATCCTAATCAA
ACGAAGGCGACAGAAGATCCGGAAGTATACCATGCGTAGGCTGCTGCAGGAGACCGAGGTGAGGCGTGCC
GAGGGCCTCCTAGCACCCCCTTGGCTTTGCAGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR210207 representing BC046811
Red=Cloning site Green=Tags(s)

MELAAWCRWGFLLALLSPGAAGTQVCTGTDMKLRLPASPETHLDMLRHLYQGCQVVQGNLELTYLPANAS
LSFLQDIQEVQGYMLIAHNRVKHVPLQRLRIVRGTQLFEDKYALAVLDNRDPLDNVTTAAPGRTPEGLRE
LQLRSLTEILKGGVLIRGNPQLCYQDMVLWKDVLRKNNQLAPVDMDTNRSRACPPCAPTCKDNHCWGESP
EDCQILTGTICTSGCARCKGRLPTDCCHEQCAAGCTGPKHSDCLACLHFNHSGICELHCPALITYNTDTF
ESMLNPEGRYTFGASCVTTCPYNYLSTEVGSCTLVCPPNNQEVTAEDGTQRCEKCSKPCAGVCYGLGMEH
LRGARAITSDNIQEFAGCKKIFGSLAFLPESFDGNPSSGVAPLKPEHLQVFETLEEITGYLYISAWPESF
QDLSVFQNLRVIRGRILHDGAYSLTLQGLGIHSLGLRSLRELGSGLALIHRNTHLCFVNTVPWDQLFRNP
HQALLHSGNRPEEACGLEGLVCNSLCARGHCWGPGPTQCVNCSQFLRGQECVEECRVWKGLPREYVRGKH
CLPCHPECQPQNSSETCYGSEADQCEACAHYKDSSSCVARCPSGVKPDLSYMPIWKYPDEEGICQPCPIN
CTHSCVDLDERGCPAEQRASPVTFIIATVVGVLLFLIIVVVIGILIKRRRQKIRKYTMRRLLQETEVRRA
EGLLAPPWLCS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC046811
ORF Size 2133 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC046811, AAH46811
RefSeq Size 4694 bp
RefSeq ORF 2135 bp
Locus ID 13866
MW 172 kDa
Gene Summary Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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