United States
Germany
Japan
United Kingdom
China
Lipe (NM_001039507) Mouse Tagged ORF Clone
CAT＃: MR210507
- TrueORF®
Lipe (Myc-DDK-tagged) - Mouse lipase, hormone sensitive (Lipe), transcript variant 2
"NM_001039507" in other vectors (3)
Need custom modification / cloning service?
Get a free quote
CNY 8,352.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 4933403G17Rik; HSL |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210507 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATTTACGCACGATGACACAGTCGCTGGTGACACTCGCAGAAGACAATATGGCCTTCTTCTCAAGCC AGGGCCCAGGAGAGACAGCACGGCGGCTGTCTAATGTCTTTGCAGGTGTTCGGGAACAGGCACTGGGGCT GGAACCAACCCTAGGCCAACTGTTGGGTGTGGCACACCATTTTGACCTGGACACAGAGACACCAGCCAAC GGATACCGTAGTTTGGTGCACACAGCCCGATGCTGCCTGGCACACCTACTACACAAATCCCGCTATGTGG CTTCTAACCGCAAAAGTATCTTCTTCCGTGCCAGCCACAACCTAGCAGAGCTGGAGGCCTACCTGGCCGC CCTCACCCAGCTCCGTGCTATGGCCTACTATGCCCAGCGCCTGCTGACCATCAACCGACCAGGAGTGCTC TTCTTCGAGGGTGATGAAGGACTCACCGCTGACTTCCTGCAAGAGTATGTCACGCTACACAAAGGCTGCT TCTACGGCCGCTGCCTGGGCTTCCAGTTCACACCTGCCATCCGGCCGTTCCTGCAGACTCTCTCCATCGG GCTGGTGTCCTTCGGGGAGCACTACAAACGCAACGAGACAGGCCTCAGTGTGACCGCCAGTTCCCTCTTT ACCGGTGGCCGATTCGCCATAGACCCAGAGTTGCGTGGGGCTGAATTTGAACGCATCATACAGAACCTGG ATGTGCACTTCTGGAAAGCCTTCTGGAACATCACTGAGATTGAGGTGCTGTCGTCTCTGGCCAACATGGC ATCAACCACTGTGAGGGTAAGCCGCCTGCTCAGCTTGCCACCTGAGGCCTTTGAGATGCCACTCACCTCT GATCCCAGGCTCACAGTTACCATCTCACCTCCCTTGGCACACACGGGACCAGCTCCTGTGCTAGCCAGGC TCATCTCCTATGACCTACGGGAAGGACAGGACAGCAAGGTACTCAACAGCCTGGCAAAATCTGAGGGCCC ACGCCTGGAGCTGCGCCCACGGCCTCACCAAGCACCCCGTTCACGGGCCCTGGTTGTTCACATCCACGGA GGCGGCTTTGTGGCACAGACCTCTAAATCCCACGAGCCCTACCTCAAGAACTGGGCCCAGGAGCTAGGAG TCCCTATCTTCTCCATCGACTACTCCCTGGCCCCCGAGGCTCCCTTTCCCCGAGCGCTGGAGGAGTGTTT TTTTGCCTACTGCTGGGCTGTCAAGCACTGTGACCTGCTTGGTTCAACTGGAGAGCGGATATGCCTTGCA GGGGACAGTGCAGGTGGGAATCTCTGCATCACTGTGTCCCTTCGGGCAGCAGCCTATGGAGTGAGGGTGC CAGATGGCATCATGGCAGCCTACCCAGTTACCACCCTGCAGTCCTCTGCTTCTCCCTCTCGTCTGCTGAG CCTCATGGACCCTCTTCTACCACTGAGCGTACTCTCTAAGTGTGTCAGTGCCTATTCAGGGACAGAGGCA GAGGACCATTTTGACTCAGACCAGAAGGCACTAGGCGTGATGGGGCTGGTGCAGAGAGACACTTCGCTGT TCCTCAGAGACCTCCGACTGGGTGCCTCCTCATGGCTCAACTCCTTCCTGGAACTAAGTGGACGCAAGCC CCAAAAGACCACATCGCCCACAGCAGAGTCTGTGCGCCCCACGGAGTCTATGCGCAGGAGTGTGTCTGAG GCAGCCCTGGCCCAGCCTGAGGGCTTACTGGGCACAGATACCTTGAAGAAGCTGACAATAAAGGACTTGA GCAACTCAGAGCCTTCAGACAGCCCCGAGATGTCACAGTCAATGGAGACACTTGGCCCCTCCACACCCTC TGATGTCAACTTTTTTCTGCGGCCTGGGAATTCCCAGGAAGAGGCTGAAGCCAAAGATGAAGTGAGACCC ATGGACGGAGTCCCCCGCGTGCGCGCTGCTTTCCCTGAGGGGTTTCACCCCCGGCGCTCAAGCCAAGGTG TCCTCCACATGCCCCTCTACACGTCACCCATAGTCAAGAACCCCTTCATGTCTCCTCTGCTGGCCCCTGA CAGCATGCTGAAGACCTTGCCGCCTGTGCACCTTGTGGCTTGCGCTCTGGACCCCATGCTAGATGACTCG GTCATGTTCGCGCGGCGACTGCGCGACCTGGGCCAGCCGGTGACGCTGAAAGTGGTAGAAGATCTGCCGC ATGGCTTCCTGAGCCTGGCGGCACTGTGTCGCGAGACCCGGCAGGCCACGGAGTTCTGCGTGCAGCGCAT CCGGCTGATCCTCACCCCGCCTGCTGCACCACTGAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210507 protein sequence
Red=Cloning site Green=Tags(s) MDLRTMTQSLVTLAEDNMAFFSSQGPGETARRLSNVFAGVREQALGLEPTLGQLLGVAHHFDLDTETPAN GYRSLVHTARCCLAHLLHKSRYVASNRKSIFFRASHNLAELEAYLAALTQLRAMAYYAQRLLTINRPGVL FFEGDEGLTADFLQEYVTLHKGCFYGRCLGFQFTPAIRPFLQTLSIGLVSFGEHYKRNETGLSVTASSLF TGGRFAIDPELRGAEFERIIQNLDVHFWKAFWNITEIEVLSSLANMASTTVRVSRLLSLPPEAFEMPLTS DPRLTVTISPPLAHTGPAPVLARLISYDLREGQDSKVLNSLAKSEGPRLELRPRPHQAPRSRALVVHIHG GGFVAQTSKSHEPYLKNWAQELGVPIFSIDYSLAPEAPFPRALEECFFAYCWAVKHCDLLGSTGERICLA GDSAGGNLCITVSLRAAAYGVRVPDGIMAAYPVTTLQSSASPSRLLSLMDPLLPLSVLSKCVSAYSGTEA EDHFDSDQKALGVMGLVQRDTSLFLRDLRLGASSWLNSFLELSGRKPQKTTSPTAESVRPTESMRRSVSE AALAQPEGLLGTDTLKKLTIKDLSNSEPSDSPEMSQSMETLGPSTPSDVNFFLRPGNSQEEAEAKDEVRP MDGVPRVRAAFPEGFHPRRSSQGVLHMPLYTSPIVKNPFMSPLLAPDSMLKTLPPVHLVACALDPMLDDS VMFARRLRDLGQPVTLKVVEDLPHGFLSLAALCRETRQATEFCVQRIRLILTPPAAPLN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001039507 |
| ORF Size | 2280 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001039507.2 |
| RefSeq Size | 2785 bp |
| RefSeq ORF | 2280 bp |
| Locus ID | 16890 |
| UniProt ID | P54310 |
| MW | 83.3 kDa |
| Gene Summary | In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200824 | Lipe (untagged) - Mouse lipase, hormone sensitive (Lipe), transcript variant 2, (10ug) |
CNY 5,568.00 |
|
| MR210507L3 | Lenti ORF clone of Lipe (Myc-DDK-tagged) - Mouse lipase, hormone sensitive (Lipe), transcript variant 2 |
CNY 7,700.00 |
|
| MR210507L4 | Lenti ORF clone of Lipe (mGFP-tagged) - Mouse lipase, hormone sensitive (Lipe), transcript variant 2 |
CNY 7,700.00 |
