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Tle1 (NM_011599) Mouse Tagged ORF Clone
CAT＃: MR210559
- TrueORF®
Tle1 (Myc-DDK-tagged) - Mouse transducin-like enhancer of split 1, homolog of Drosophila E(spl) (Tle1)
ORF Plasmid: tGFP
"NM_011599" in other vectors (3)
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CNY 5,640.00
CNY 5,800.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | C230057C06Rik; Estm14; Grg-1; Grg1; Tle4l |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210559 representing NM_011599
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCCCGCAGAGCCGGCACCCAACGCCGCACCAAGCTGCAGGCCAGCCCTTTAAGTTCACTATCCCGG AGTCTCTGGACCGGATTAAAGAGGAATTCCAGTTCCTGCAGGCGCAGTATCACAGTCTTAAATTGGAGTG TGAGAAACTGGCAAGTGAAAAGACAGAAATGCAGAGGCACTACGTGATGTATTATGAAATGTCATATGGA TTAAACATTGAAATGCACAAACAGACTGAAATCGCCAAGAGATTGAACACCATTTGTGCCCAAGTCATCC CATTTCTGTCTCAGGAACATCAACAACAGGTGGCCCAGGCTGTGGAACGTGCCAAACAGGTGACCATGGC AGAGTTGAATGCCATCATCGGGCAGCAGCAGTTGCAAGCTCAGCATCTCTCCCATGGCCATGGACCCCCA GTACCTCTCACGCCTCACCCTTCAGGACTTCAGCCTCCTGGCATCCCGCCCCTTGGGGGCAGTGCCAGCC TTCTCGCGCTGTCTAGTGCTCTGAGTGGGCAGTCTCACTTGGCAATAAAAGATGACAAGAAGCACCATGA TGCAGAGCGCCACAGAGACAGAGAGCCTGGCACGAGTAATTCCCTCTTGGTCCCAGACAGCCTAAGAGGC ACAGATAAGCGCAGAAATGGTCCAGAGTTTTCCAGTGACATCAAAAAAAGGAAGGTGGATGATAAGGATA ACTATGACAGTGATGGGGACAAGAGTGATGACAACTTAGTTGTGGATGTGTCTAACGAGGACCCTTCTTC TCCACATGCAAGCCCCACACACTCACCCCGGGAAAACGGAATTGACAAAAACCGTCTGCTGAAGAAAGAT GCTTCAGGTAGCCCGGCATCCACAGCCTCCTCTGGAAGTTCCTCTTCCCTGAAATCCAAAGAAGTGAGCC TGCATGAAAAAGCCAACACTCCTGTTCTGAAATCCAGCACACCGACGCCTCGGAGCGACATGCCAACCCC AGGCACCAGCGCTACTCCAGGCCTCCGTCCAGGTCTTGGCAAGCCTCCAGCCATGGAACCCCTTGTCAAC CAAGCAGCAGCTGGCCTGAGGACGCCCCTGGCGGTGCCTGGCCCATACCCTGCCCCCTTTGGCATGGTGC CCCATGCGGGCATGAACGGAGAGCTGACCAGCCCTGGTGCTGCCTATGCAGGTCTACACAGCATGTCTCC ACAGATGAGCGCTGCAGCTGCTGCAGCTGCTGCTGCTGTGGTGGCCTATGGGCGCTCCCCAATGGTTGGT TTTGATCCTCCTCCTCACATGAGAGTACCTTCTATCCCCCCCAACCTGGCAGGAATACCTGGAGGGAAAC CAGCATACTCCTTCCACGTTACTGCTGATGGCCAAATGCAGCCTGTTCCTTTTCCCCCTGATGCCCTCAT TGGACCTGGGATTCCCCGACATGCTCGGCAGATCAACACCCTCAACCACGGGGAGGTGGTGTGTGCAGTG ACCATCAGCAACCCCACAAGGCACGTGTACACAGGTGGCAAGGGCTGCGTTAAGGTGTGGGACATCAGCC ACCCCGGCAACAAGAGCCCCGTCTCTCAGCTGGATTGTCTGAATAGAGATAACTACATCCGTTCCTGTAA ATTGCTACCTGATGGCTGTACTCTCATAGTGGGAGGGGAAGCCAGTACTCTATCCATCTGGGACTTGGCA GCTCCAACTCCCCGCATAAAGGCAGAGCTGACATCCTCAGCCCCTGCCTGTTATGCTCTGGCCATCAGCC CCGACTCCAAGGTCTGCTTCTCATGCTGCAGTGACGGCAACATCGCAGTGTGGGACCTGCACAACCAGAC CCTGGTGAGGCAATTCCAGGGCCACACAGACGGAGCCAGCTGTATTGACATTTCTAATGATGGCACCAAG CTCTGGACAGGCGGTTTAGACAACACTGTGAGGTCCTGGGACCTGAGAGAAGGGAGGCAGCTGCAGCAGC ATGACTTCACTTCACAGATCTTCTCTTTGGGATATTGCCCAACTGGGGAGTGGCTTGCTGTGGGCATGGA AAGCAGCAACGTGGAAGTTCTGCATGTGAACAAGCCTGACAAGTACCAGCTGCACCTCCACGAGAGCTGC GTGCTGTCCCTAAAGTTTGCTTATTGTGGCAAATGGTTTGTGAGTACTGGAAAAGACAACCTCCTAAATG CCTGGCGGACACCATATGGAGCCAGCATCTTCCAGTCCAAAGAATCTTCATCAGTGCTTAGCTGTGACAT CTCTGTGGATGATAAATACATAGTCACTGGCTCAGGGGACAAAAAGGCTACCGTTTATGAGGTCATCTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210559 representing NM_011599
Red=Cloning site Green=Tags(s) MFPQSRHPTPHQAAGQPFKFTIPESLDRIKEEFQFLQAQYHSLKLECEKLASEKTEMQRHYVMYYEMSYG LNIEMHKQTEIAKRLNTICAQVIPFLSQEHQQQVAQAVERAKQVTMAELNAIIGQQQLQAQHLSHGHGPP VPLTPHPSGLQPPGIPPLGGSASLLALSSALSGQSHLAIKDDKKHHDAERHRDREPGTSNSLLVPDSLRG TDKRRNGPEFSSDIKKRKVDDKDNYDSDGDKSDDNLVVDVSNEDPSSPHASPTHSPRENGIDKNRLLKKD ASGSPASTASSGSSSSLKSKEVSLHEKANTPVLKSSTPTPRSDMPTPGTSATPGLRPGLGKPPAMEPLVN QAAAGLRTPLAVPGPYPAPFGMVPHAGMNGELTSPGAAYAGLHSMSPQMSAAAAAAAAAVVAYGRSPMVG FDPPPHMRVPSIPPNLAGIPGGKPAYSFHVTADGQMQPVPFPPDALIGPGIPRHARQINTLNHGEVVCAV TISNPTRHVYTGGKGCVKVWDISHPGNKSPVSQLDCLNRDNYIRSCKLLPDGCTLIVGGEASTLSIWDLA APTPRIKAELTSSAPACYALAISPDSKVCFSCCSDGNIAVWDLHNQTLVRQFQGHTDGASCIDISNDGTK LWTGGLDNTVRSWDLREGRQLQQHDFTSQIFSLGYCPTGEWLAVGMESSNVEVLHVNKPDKYQLHLHESC VLSLKFAYCGKWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDISVDDKYIVTGSGDKKATVYEVIY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011599 |
| ORF Size | 2310 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011599.5 |
| RefSeq Size | 4369 bp |
| RefSeq ORF | 2313 bp |
| Locus ID | 21885 |
| UniProt ID | Q62440 |
| MW | 83.5 kDa |
| Gene Summary | Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG210559 | Tle1 (tGFP-tagged) - Mouse transducin-like enhancer of split 1, homolog of Drosophila E(spl) (Tle1) |
CNY 6,370.00 |
|
| MR210559L3 | Lenti ORF clone of Tle1 (Myc-DDK-tagged) - Mouse transducin-like enhancer of split 1, homolog of Drosophila E(spl) (Tle1) |
CNY 7,700.00 |
|
| MR210559L4 | Lenti ORF clone of Tle1 (mGFP-tagged) - Mouse transducin-like enhancer of split 1, homolog of Drosophila E(spl) (Tle1) |
CNY 7,700.00 |
