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Kdm2b (NM_013910) Mouse Tagged ORF Clone
CAT＃: MR210589
- TrueORF®
Kdm2b (Myc-DDK-tagged) - Mouse lysine (K)-specific demethylase 2B (Kdm2b), transcript variant 2
"NM_013910" in other vectors (3)
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CNY 5,688.00
CNY 6,750.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Cxxc2; Fbl10; Fbxl1; Fbxl10; Jhdm1b; PCCX2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210589 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCATGTCCGTGAGCGCCGAGGACGACGACTATGAATCGGAGCCCGACCAGAACCGGGTGGTGGGTC GGCCTAAGGGCAAGTTGGGCCCGGCCTCAGCGGTGAAGTTGGCTGCCAACCGAACAACAGCAGGAGCTCG CAGGCGCCGGACGCGATGCCGCAAGTGCGAGGCCTGCCTGCGGACGGAGTGTGGAGAGTGCCACTTTTGC AAGGACATGAAGAAGTTTGGAGGTCCTGGGCGCATGAAGCAGAGCTGCATCATGCGGCAGTGCATCGCGC CAGTGCTGCCCCACACCGCCGTGTGCCTTGTGTGTGGCGAGGCAGGGAAGGAGGACACAGTGGAAGAGGA AGAAGGCAAGTTTAACCTCATGCTCATGGAATGCTCCATCTGCAACGAGATCATCCACCCTGGATGCCTT AAGATTAAGGAATCGGAGGGTGTGGTCAACGATGAGCTTCCCAACTGCTGGGAGTGTCCGAAGTGTAACC ATGCCGGCAAGACCGGGAAACAAAAGCGTGGCCCTGGCTTTAAGTATGCCTCCAACCTGCCTGGCTCCTT GCTCAAGGAGCAGAAGATGAACCGGGACAACAAGGAAGGGCAAGAGCCTGCCAAGCGGAGAAGTGAGTGT GAAGAGGCTCCCCGTCGGAGGTCAGACGAGCACCCCAAAAAGGTGCCTGCAGATGGCATCCTCCGCCGAA AGTCTGATGATGTGCACCTGAGGAGGAAGCGGAAATACGAGAAGCCCCAAGAGCTGAGTGGACGCAAGCG AGCCTCGTCGCTTCAAACGTCCCCCGGTTCCTCCTCTCACCTCTCGCCGAGGCCCCCTCTAGGCAGCAGT CTCAGCCCTTGGTGGAGATCCAGTCTCACTTACTTCCAGCAGCAGCTAAAACCTGGCAAAGAAGATAAGC TTTTCAGGAAAAAGCGGCGGTCCTGGAAGAACGCTGAGGATCGTCTGTCACTGGCCAACAAGCCCCTTCG GCGCTTTAAGCAGGAACCGGAGGACGATCTGCCTGAGGCACCTCCTAAGACCCGGGAGAGTGATCAGTCA CGTTCCAGCTCACCCACTGCTGGTCCCAGCACTGAGGGAGCTGAAGGCCCAGAAGAGAAGAAAAAGGTGA AGATGCGCCGGAAGCGGCGACTTGTTAACAAGGAGCTGAGCAAAGAGCTAAGCAAGGAGCTCAACCATGA GATCCAAAAGACGGAGAGCACCCTGGCTCACGAGAGCCAGCAGCCCATCAAGTCAGAGCCTGAGAGCGAG AACGACGAGCCCAAGAGGCCCTTAAGCCACTGCGAGCGCCCCCACCGCTTCAGCAAAGGGCTCAACGGCA CACCTCGGGAGCTGCGGCACTCGCTGGGACCTGGCCTGCGTAGTCCACCTCGTGTTATGTCCCGGCCCCC GCCCTCTGCATCCCCACCCAAGTGCATCCAGATGGAGCGTCACGTGATCCGGCCACCGCCCATCAGCCCC CCACCTGACTCGCTGCCCCTGGATGATGGAGCAGCCCACGTCATGCATAGGGAGGTGTGGATGGCAGTCT TCAGCTACCTCAGCCACCGAGACCTGTGTGTCTGCATGCGGGTCTGCAGGACCTGGAACCGCTGGTGCTG CGATAAGCGGTTGTGGACCCGCATCGACCTGAACCGCTGCAAGTCCATCACACCCCTGATGCTGAGCGGT ATCATCCGGCGACAGCCTGTCTCCCTTGACCTCAGTTGGACCAACATCTCCAAGAAGCAGCTGAGTTGGC TCATCAACCGGTTGCCTGGGCTCCGAGACTTGGTGCTGTCAGGCTGCTCATGGATCGCTGTCTCAGCCCT CTGTAGCTCCAGTTGTCCATTGCTCCGGACCCTGGATGTCCAGTGGGTAGAAGGACTAAAGGATGCCCAG ATGAGGGATCTCCTGTCTCCACCCACAGACAACAGGCCAGGTCAGATGGACAATCGGAGCAAGCTCCGGA ACATTGTAGAGCTGCGCCTAGCTGGCCTGGACATCACAGATGTCTCCCTGCGGCTCATTATTCGCCATAT GCCCCTGCTCTCGAAGCTCCAACTCAGTTACTGTAACCACATCAATGACCAGTCCATCAACCTGCTCACT GCCGTCGGCACCACCACCCGAGACTCGCTGACAGAGGTCAACCTATCAGACTGTAATAAGGTAACTGACC TGTGCCTGTCCTTCTTCAAACGCTGTGGAAATATCTGTCATATTGACCTGAGGTACTGCAAGCAAGTCAC CAAGGAAGGCTGTGAGCAATTCATAGCTGAAATGTCTGTGAGTGTCCAATTTGGGCAAGTGGAAGAGAAA CTCCTGCAAAAACTAAGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210589 protein sequence
Red=Cloning site Green=Tags(s) MAMSVSAEDDDYESEPDQNRVVGRPKGKLGPASAVKLAANRTTAGARRRRTRCRKCEACLRTECGECHFC KDMKKFGGPGRMKQSCIMRQCIAPVLPHTAVCLVCGEAGKEDTVEEEEGKFNLMLMECSICNEIIHPGCL KIKESEGVVNDELPNCWECPKCNHAGKTGKQKRGPGFKYASNLPGSLLKEQKMNRDNKEGQEPAKRRSEC EEAPRRRSDEHPKKVPADGILRRKSDDVHLRRKRKYEKPQELSGRKRASSLQTSPGSSSHLSPRPPLGSS LSPWWRSSLTYFQQQLKPGKEDKLFRKKRRSWKNAEDRLSLANKPLRRFKQEPEDDLPEAPPKTRESDQS RSSSPTAGPSTEGAEGPEEKKKVKMRRKRRLVNKELSKELSKELNHEIQKTESTLAHESQQPIKSEPESE NDEPKRPLSHCERPHRFSKGLNGTPRELRHSLGPGLRSPPRVMSRPPPSASPPKCIQMERHVIRPPPISP PPDSLPLDDGAAHVMHREVWMAVFSYLSHRDLCVCMRVCRTWNRWCCDKRLWTRIDLNRCKSITPLMLSG IIRRQPVSLDLSWTNISKKQLSWLINRLPGLRDLVLSGCSWIAVSALCSSSCPLLRTLDVQWVEGLKDAQ MRDLLSPPTDNRPGQMDNRSKLRNIVELRLAGLDITDVSLRLIIRHMPLLSKLQLSYCNHINDQSINLLT AVGTTTRDSLTEVNLSDCNKVTDLCLSFFKRCGNICHIDLRYCKQVTKEGCEQFIAEMSVSVQFGQVEEK LLQKLS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_013910 |
| ORF Size | 2331 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_013910.2, NP_038938.1 |
| RefSeq Size | 3541 bp |
| RefSeq ORF | 2331 bp |
| Locus ID | 30841 |
| UniProt ID | Q6P1G2 |
| MW | 88 kDa |
| Gene Summary | The protein encoded by this gene is a H3K36-specific histone demethylase, which contains an N-terminal jumonji C domain, a CxxC zinc finger domain, a plant homeodomain finger, an F-box, and eight leucine-rich repeats. Amongst its demonstrated functions, this protein plays roles in the suppression of premature cellular senescence, leukemia maintenance and development, maintenance of mouse embryonic stem cell pluripotency, and induced pluripotent stem cell generation. Mice homozygous for a targeted deletion of the zinc finger domain display embryonic lethality with development ceasing at approximately 7 to 8 days post coitum, demonstrating an essential role in early development. A pseudogene of this gene is found on chromosome 4. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC203452 | Kdm2b (untagged) - Mouse lysine (K)-specific demethylase 2B (Kdm2b), transcript variant 2, (10ug) |
CNY 5,688.00 |
|
| MR210589L3 | Lenti ORF clone of Kdm2b (Myc-DDK-tagged) - Mouse lysine (K)-specific demethylase 2B (Kdm2b), transcript variant 2 |
CNY 8,650.00 |
|
| MR210589L4 | Lenti ORF clone of Kdm2b (mGFP-tagged) - Mouse lysine (K)-specific demethylase 2B (Kdm2b), transcript variant 2 |
CNY 8,650.00 |
