Iqce (NM_028833) Mouse Tagged ORF Clone

CAT＃: MR210601

TrueORF^®

Iqce (Myc-DDK-tagged) - Mouse IQ motif containing E (Iqce)

ORF Plasmid: DDK tGFP

Datasheet

SDS

Plasmid Map

"NM_028833" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 6,272.00

货期*

现货

规格

Product images

经常一起买 (4)

PS100001

pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug

CNY 5,420.00

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

TA592569S

DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags

CNY 300.00

【特别福利】购一抗正装产品，免费领10ul抗体试用装

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	1700028P05Rik; mKIAA1023
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR210601 representing NM_028833 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCCTGGGCACCACAGATATAGCCTCGGAGACGGGAGATGACAGTCTGTCTGCTATTACCTTTGAAT CTGACATTGAGTCGAAAACAAAAAGGAAAAGTTTCCACAAACCTCCTTCCACATCACCAAAGTCGCCCTA CTACTCCAAACCCAGAAAAGTGACCTCCTGGCGATCTCTGAAGACGGCAGGGAGCATGCCTCTGAGTAGC AGAATGTCCTTGACCCCACAGAAGCTGTGGCTGGGATCCTCGAAACAGGGTAGTGTGGCCCAACCCCCGA GCCCCACGCTTACCTCGGAGCACGCGTGGACCCACCCCCCCAGCTGCACTCCCGACTACCTGACAGAAGC CGTGAGAGCGAAGAGGGCAGACCTCAGGCGTTCTGGCAGCCATGGTCACGTCTCTGGGACTTCCGTCTAC AGAGAGAAGGAGGACATGTACGACGAGATCATCGAGTTGAAGAAGTCCTTACATATGCAGAAAAGTGATG TGGATCTGATGAGAACAAAGCTTCGGCGCTTGGAAGAGGAGAACAGCAGAAAGGATCGGCAGATAGAACA GCTTCTGGATCCAAGTCGAGGCCCAGACTTCGTGCGAACCTTGGCAGAGAAGAAGCCTGACACGGGCTGG GTCATCACCGGGCTCAAACAAAGGATCTTCAGGCTGGAGCAGCAATGCAAGGAGAAGGACAACACTATCA ACAAACTGCAGACGGACATGAAGACCACCAACCTGGAGGAGATGCGCATTGCCATGGAAACATACTACGA AGAGATCCATCGTCTGCAGACCCTCCTGGCGAGTTCTGAAGCCACAGGAAAGAAGCCCATGGTGGAGAAG AAACTGGGTGTGAAGAGGCAGAAGAAGATGAGCAGTGCCCTGCTGAACCTAACACGGAGTGTGCAGGAGC TGACCGAGGAGAACCAGAGCCTGAAGGAGGACCTGGACCGCATGCTGAGCAATTCACCCACCATCTCCAA AATAAAGGGTTACGGGGACTGGAGCAAGCCCCGGCTGCTGAGACGCATCGCAGAGCTAGAAAAGAAAGTG AGTTCCTCAGAAAGCCCAAAGCAATCGACTTCAGAGCTGGTGAATCCGAATCCCCTGGTCCGCTCCCCAT CCAACATCTCAGTGCAAAAGCAGCCGAAAGGGGACCAGTCCCCAGAGGACCTGCCCAAGGTGGCCCCGTG TGAGGAACAAGAGCATCTCCAAGGAACCGTGAAGAGCCTGAGAGAGGAGCTGGGTGCACTGCAGGAGCAG CTGCTGGAGAAAGACCTGGAGATGAAGCAGCTGCTACAGTCCAAGATCGACCTGGAGAAGGAGTTGGAGA CAGCCAGGGAAGGCGAGAAGGGGAGGCAGGAGCAGGAGCAGGCTTTGAGGGAGGAAGTTGAAGCACTCAC CAAAAAATGTCAAGAACTGGAAGAAGCTAAGAGAGAAGAGAAAAACTCCTTCGTAGCAGTGACTCATGAG GCTCATCCAGAGCTCCATGCCCCCTCGCCCTGCAGCAGGCACTCCGAGCCAGACTCTGACAATAGTGCTG GTGAGGAGGGTAGCTCCCAACCCCCTGCCCCATGCTCTGAAGAGAGGAGAGAGGCTGCCATTAGGACCCT GCAGGCGCAGTGGAAGGCCCACAGGCGCAAGAAAAGAGAAGCAGCTCTGGATGAGGCAGCGACTGTGCTC CAGGCAGCCTTCAGGGGACACCTAGCTCGTTCGAAGCTTGTACGCAGCAAAGTCCCAGACTCCAGGTCTC CCAGCCTGCCGGGCCTTCTCAGCCCTCTTAACCAGAGTTCTCCTGCGCCCCGTGTCCTAAGCCCCATCTC CCCGGCTGAAGAGAACCCAACACAAGAGGAGGCTGTCATCGTTATCCAGTCCATCCTCCGTGGATACCTG GCCCAGGCCAGGTTCATTGCCAGCTGCTGTCGAGAGATTGCGGCGTCCTCTCAGAGGGAAACTGTTTCAT TGACACCCTCAGGATCTGCCTCCCCACCCTCCCTCAGAGCTTCTCCCGGAGTGATCCGCAAGGAGCTCTG TGCCTCGGAGGAGCTGAGGGAGACCTCTGCATCTGAACCTGCCCCCTCCGTCCCCTACTCCGCCCAAGGT GGCCACGGGGACTGCCCGTCCTCATCCAGTCTGGAGGCTGTGCCCAGTATGAAGGATGCCATGTGTGAGG AAAGGAGCAGCAGCCCTAGAAGCGCAGGGCCATCCTTGGCTGAGCCCTCTCCCCCAGAACTGCAGCCCTT GTCACCACCACCTGTGGAGGACATCTGCTCGGATGACTCAGATGACATTATCTTTTCGCCATTTCTGCCC AGGAAGAAAAGCCCCTCCCCGTTC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR210601 representing NM_028833 Red=Cloning site Green=Tags(s) MSLGTTDIASETGDDSLSAITFESDIESKTKRKSFHKPPSTSPKSPYYSKPRKVTSWRSLKTAGSMPLSS RMSLTPQKLWLGSSKQGSVAQPPSPTLTSEHAWTHPPSCTPDYLTEAVRAKRADLRRSGSHGHVSGTSVY REKEDMYDEIIELKKSLHMQKSDVDLMRTKLRRLEEENSRKDRQIEQLLDPSRGPDFVRTLAEKKPDTGW VITGLKQRIFRLEQQCKEKDNTINKLQTDMKTTNLEEMRIAMETYYEEIHRLQTLLASSEATGKKPMVEK KLGVKRQKKMSSALLNLTRSVQELTEENQSLKEDLDRMLSNSPTISKIKGYGDWSKPRLLRRIAELEKKV SSSESPKQSTSELVNPNPLVRSPSNISVQKQPKGDQSPEDLPKVAPCEEQEHLQGTVKSLREELGALQEQ LLEKDLEMKQLLQSKIDLEKELETAREGEKGRQEQEQALREEVEALTKKCQELEEAKREEKNSFVAVTHE AHPELHAPSPCSRHSEPDSDNSAGEEGSSQPPAPCSEERREAAIRTLQAQWKAHRRKKREAALDEAATVL QAAFRGHLARSKLVRSKVPDSRSPSLPGLLSPLNQSSPAPRVLSPISPAEENPTQEEAVIVIQSILRGYL AQARFIASCCREIAASSQRETVSLTPSGSASPPSLRASPGVIRKELCASEELRETSASEPAPSVPYSAQG GHGDCPSSSSLEAVPSMKDAMCEERSSSPRSAGPSLAEPSPPELQPLSPPPVEDICSDDSDDIIFSPFLP RKKSPSPF SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII Cloning Scheme for this gene Plasmid Map
ACCN	NM_028833
ORF Size	2334 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_028833.3, NP_083109.2
RefSeq Size	4359 bp
RefSeq ORF	2337 bp
Locus ID	74239
UniProt ID	Q6PCQ0
MW	86.4 kDa
Gene Summary	Component of the EvC complex that positively regulates ciliary Hedgehog (Hh) signaling (PubMed:24582806). Required for proper limb morphogenesis (By similarity).[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
MC221507	Iqce (untagged) - Mouse IQ motif containing E (Iqce), (10ug)	10 ug 产品选项验证失败所有选项都是必需的	CNY 9,980.00
MG210601	Iqce (tGFP-tagged) - Mouse IQ motif containing E (Iqce)	10 ug 产品选项验证失败所有选项都是必需的	CNY 6,370.00
MR210601L3	Lenti ORF clone of Iqce (Myc-DDK-tagged) - Mouse IQ motif containing E (Iqce)	10 ug 产品选项验证失败所有选项都是必需的	CNY 7,700.00
MR210601L4	Lenti ORF clone of Iqce (mGFP-tagged) - Mouse IQ motif containing E (Iqce)	10 ug 产品选项验证失败所有选项都是必需的	CNY 7,700.00

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