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Postn (BC031449) Mouse Tagged ORF Clone
CAT＃: MR210633
- TrueORF®
Postn (Myc-DDK-tagged) - Mouse periostin, osteoblast specific factor (cDNA clone MGC:25368 IMAGE:4457222)
ORF Plasmid: tGFP
"BC031449" in other vectors (4)
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CNY 5,736.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | OSF-2, Osf2, Periostin, PN |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210633 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTTCCTCTCCTGCCCTTATATGCTCTGCTGCTGCTGTTCCTGTGTGATATTAACCCTGCAAATGCCA ACAGTTACTATGACAAGGTCCTGGCTCACAGCCGCATCAGGGGTCGGGATCAGGGCCCAAACGTCTGTGC CCTCCAGCAAATTCTGGGCACCAAAAAGAAATACTTCAGCTCCTGTAAGAACTGGTATCAAGGTGCTATC TGCGGGAAGAAAACCACTGTGCTATATGAATGCTGCCCTGGCTATATGAGAATGGAAGGGATGAAAGGCT GCCCCGCAGTGATGCCTATTGACCATGTTTATGGCACGCTGGGCATTGTGGGAGCCACTACCACTCAGCA CTACTCCGATGTCTCGAAGCTGAGAGAAGAGATTGAAGGAAAAGGGTCATGCACGTACTTCGCGCCGAGT AACGAGGCTTGGGAGAACCTGGATTCTGACATTCGCAGAGGACTGGAGAACAATGTCAATGTTGAGCTAC TGAATGCCTTACACAGCCACATGGTTAATAAGAGAATGTTAACCAAGGACCTGAAACACGGCATGGTTAT TCCTTCAATGTACAACAATCTGGGGCTTTTTATTAACCATTATCCCAATGGGGTTGTCACTGTGAACTGT GCTCGAGTCATCCATGGGAACCAGATTGCCACAAATGGTGTCGTCCATGTCATTGACCGTGTCCTGACAC AAATTGGTACCTCCATCCAAGACTTCCTTGAAGCAGAAGACGACCTTTCATCATTTAGAGCAGCCGCCAT CACCTCTGACCTCTTGGAGTCCCTTGGAAGAGATGGTCACTTCACGCTCTTTGCTCCCACCAATGAAGCT TTCGAGAAACTGCCACGAGGTGTCCTAGAAAGGATCATGGGAGACAAAGTGGCTTCTGAAGCTCTCATGA AGTACCACATCCTAAATACCCTCCAGTGCTCTGAGGCCATCACTGGAGGAGCCGTGTTTGAGACCATGGA AGGAAACACTATTGAGATAGGGTGCGAAGGGGACAGTATCTCCATTAACGGAATCAAGATGGTGAACAAG AAAGACATTGTGACTAAGAATGGTGTCATCCACCTGATTGATGAAGTCCTCATTCCTGATTCTGCCAAAC AAGTTATTGAGCTGGCTGGAAAACAGCAAACCACTTTCACCGACCTGGTAGCCCAATTAGGCTTGGCATC CTCTCTGAAGCCAGATGGAGAGTACACCTTATTAGCACCTGTGAACAATGCGTTCTCTGATGACACTCTG AGCATGGACCAACGCCTTCTTAAGCTAATTCTGCAAAATCACATATTGAAAGTAAAAGTTGGCCTTAGCG ACCTCTACAATGGACAGATACTGGAAACCATTGGAGGCAAACAACTCCGAGTCTTTGTGTATCGGACGGC TATCTGCATAGAAAACTCATGCATGGTGAGAGGAAGCAAGCAGGGAAGGAATGGTGCCATTCACATATTC CGAGAAATCATCCAACCAGCAGAGAAATCCCTGCACGACAAGCTGCGGCAAGACAAGCGCTTTAGCATCT TCCTCAGCCTCCTTGAAGCTGCAGATTTGAAAGATCTCCTGACACAGCCCGGAGATTGGACCTTGTTTGC ACCAACCAATGATGCCTTCAAGGGAATGACTAGCGAAGAAAGGGAGCTTCTGATTGGGGATAAAAATGCT CTCCAAAACATCATTCTTTATCACCTGACCCCAGGGGTTTATATTGGAAAGGGATTCGAACCCGGAGTCA CTAATATCCTGAAGACCACACAGGGAAGCAAAATCTATCTGAAAGGAGTAAACGAAACGCTTCTAGTGAA TGAGTTGAAGTCCAAAGAATCTGACATCATGACGACAAATGGTGTCATCCACGTCGTGGACAAACTCCTC TATCCAGCAGATATTCCAGTTGGAAATGATCAGCTCTTGGAATTACTGAACAAACTGATAAAATACATCC AAATCAAGTTTGTTCGTGGCAGCACCTTCAAAGAAATCCCCATGACTGTCTATAGACCTGCAATGACGAA GATCCAAATTGAAGGTGATCCCGACTTCAGGCTGATTAAAGAAGGCGAAACGGTGACAGAAGTGATCCAC GGAGAGCCAGTCATTAAAAAGTACACCAAAATCATAGATGGAGTTCCTGTTGAAATAACTGAAAAACAGA CTCGGGAAGAACGAATCATTACAGGTCCTGAGATAAAATATACCAGGATTTCCACAGGAGGTGGAGAAAC AGGAGAGACCTTGCAGAAATTCTTGCAAAAAGACACACCTGCAAAGAAGATACCAGCCAACAAAAGGGTT CAAGGGCCTAGAAGACGATCAAGAGAAGGCCGTTCTCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210633 protein sequence
Red=Cloning site Green=Tags(s) MVPLLPLYALLLLFLCDINPANANSYYDKVLAHSRIRGRDQGPNVCALQQILGTKKKYFSSCKNWYQGAI CGKKTTVLYECCPGYMRMEGMKGCPAVMPIDHVYGTLGIVGATTTQHYSDVSKLREEIEGKGSCTYFAPS NEAWENLDSDIRRGLENNVNVELLNALHSHMVNKRMLTKDLKHGMVIPSMYNNLGLFINHYPNGVVTVNC ARVIHGNQIATNGVVHVIDRVLTQIGTSIQDFLEAEDDLSSFRAAAITSDLLESLGRDGHFTLFAPTNEA FEKLPRGVLERIMGDKVASEALMKYHILNTLQCSEAITGGAVFETMEGNTIEIGCEGDSISINGIKMVNK KDIVTKNGVIHLIDEVLIPDSAKQVIELAGKQQTTFTDLVAQLGLASSLKPDGEYTLLAPVNNAFSDDTL SMDQRLLKLILQNHILKVKVGLSDLYNGQILETIGGKQLRVFVYRTAICIENSCMVRGSKQGRNGAIHIF REIIQPAEKSLHDKLRQDKRFSIFLSLLEAADLKDLLTQPGDWTLFAPTNDAFKGMTSEERELLIGDKNA LQNIILYHLTPGVYIGKGFEPGVTNILKTTQGSKIYLKGVNETLLVNELKSKESDIMTTNGVIHVVDKLL YPADIPVGNDQLLELLNKLIKYIQIKFVRGSTFKEIPMTVYRPAMTKIQIEGDPDFRLIKEGETVTEVIH GEPVIKKYTKIIDGVPVEITEKQTREERIITGPEIKYTRISTGGGETGETLQKFLQKDTPAKKIPANKRV QGPRRRSREGRSQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC031449 |
| ORF Size | 2349 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC031449, AAH31449 |
| RefSeq Size | 3109 bp |
| RefSeq ORF | 2351 bp |
| Locus ID | 50706 |
| MW | 87 kDa |
| Gene Summary | This gene encodes a secreted extracellular matrix protein that functions in tissue development and regeneration, including wound healing and ventricular remodeling following myocardial infarction. The encoded protein binds to integrins to support adhesion and migration of epithelial cells. This protein plays a role in cancer stem cell maintenance and metastasis. Mice lacking this gene exhibit cardiac valve disease, and skeletal and dental defects. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Role of Periostin in Adhesion and Migration of Bone Remodeling Cells
,null,
PLoS ONE
,PubMed ID 26809067
[Postn]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220781 | Postn (untagged) - Mouse periostin, osteoblast specific factor (cDNA clone MGC:25368 IMAGE:4457222), (10ug) |
CNY 9,120.00 |
|
| MG210633 | Postn (tGFP-tagged) - Mouse periostin, osteoblast specific factor (cDNA clone MGC:25368 IMAGE:4457222) |
CNY 4,940.00 |
|
| MR210633L3 | Lenti ORF clone of Postn (Myc-DDK-tagged) - Mouse periostin, osteoblast specific factor (cDNA clone MGC:25368 IMAGE:4457222) |
CNY 6,370.00 |
|
| MR210633L4 | Lenti ORF clone of Postn (mGFP-tagged) - Mouse periostin, osteoblast specific factor (cDNA clone MGC:25368 IMAGE:4457222) |
CNY 8,136.00 |
