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Kifap3 (BC049100) Mouse Tagged ORF Clone
CAT＃: MR210688
- TrueORF®
Kifap3 (Myc-DDK-tagged) - Mouse kinesin-associated protein 3 (cDNA clone MGC:61298 IMAGE:5698182)
ORF Plasmid: tGFP
"BC049100" in other vectors (4)
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CNY 5,808.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | KAP3, SMAP |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210688 representing BC049100
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAAGGCGAGGACGCCAGATACCTCAAAAGGAAAGTTAAAGGGGGGAATATTGATGTACATCCATCAG AAAAAGCTCTCATTGTTCAGTATGAAGTGGAAGCTACCATTCTTGGAGAAATGGGAGATCCCATGTTGGG AGAACGAAAGGAATGCCAAAAAATCATCCGTCTGAAGAGTCTCAATGCTAACACAGACATCACCTCTCTG GCCCGGAAGGTGGTTGAGGAATGTAAGCTCATCCATCCCTCAAAGCTAAGCGAGGTGGAGCAGCTTTTGT ACTATCTACAGAACCGCCGAGACTCATTGCCGGGAAAAGAAAAAAAAGAAAAATCAAGCAAGCCTAAAGA CCCACCTCCTTTTGAAGGGATGGAGATTGACGAAGTGGCCAACATTAATGACATGGACGAATACATTGAG CTCTTGTATGAAGACATTCCAGATAAGGTTCGGGGTTCTGCCTTGATCCTACAGCTTGCTCGAAATCCTG ATAACCTGGAAGAGCTATTATTAAATGAAACTGCCCTGGGTGCGCTAGCAAGAGTCCTGAGAGAAGACTG GAAACAAAGTGTTGAGTTAGCTACAAACATCATTTATATCTTCTTTTGCTTTTCTAGCTTTTCTCATTTT CATGGACTCATCACTCACTACAAAATTGGAGCACTGTGTATGAATATCATTGATCATGAGTTAAAAAGAC ACGAGCTTTGGCAAGAAGAACTCTCTAAGAAGAAGAAAGCTGTTGATGAAGACCTTGAAAATCAAACATT GAGAAAGGATTATGACAAAACCTTTAAAAAATACCAAGGACTTGTGGTAAAACAAGAGCAGCTACTGAGA GTTGCTCTCTACTTGCTTTTGAATCTTGCGGAGGACACACGTACAGAACTGAAGATGAGGAACAAGAATA TCGTTCACATGTTGGTGAAGGCTCTTGATCGGGACAATTTTGAGCTGCTGATTCTGGTCGTGTCATTCTT AAAGAAACTGAGTATTTTTATGGAGAATAAAAATGACATGGTAGAGATGGATATTGTTGAAAAACTGGTA AAAATGATACCCTGTGAGCATGAAGATCTCCTGAATATCACCCTCCGGCTCCTCTTAAACCTCTCGTTTG ACACAGGCCTGAGGAACAAGATGGTACAAGTTGGGCTTCTCCCAAAGCTCACTGCACTCCTGGGCAATGA AAACTACAAACAAATAGCAATGTGTGTGCTTTACCATATAAGCATGGATGACCGCTTTAAGTCAATGTTT GCATATACTGACTGCATACCACAGTTAATGAAGATGCTCTTTGAATGTTCAGATGAACGAATTGACTTGG AGCTGATTTCTTTCTGCATTAATCTTGCTGCTAACAAGAGAAATGTCCAGCTCATCTGTGAAGGAAATGG GCTGAAAATGCTCATGAAAAGAGCTCTGAAGCTCAAGGACCCACTGCTGATGAAGATGATCAGAAACATC TCCCAGCATGATGGGCCCACTAAGAATTTGTTCATTGATTATGTTGGGGACCTTGCAGCCCAGATTTCCA GTGATGAAGAGGAGGAGTTTGTAATCGAGTGTTTGGGAACGCTGGCAAATCTGACAATCCCAGATCTAGA CTGGGAACTGGTCCTGAAGGAGTACAAGCTGGTTCCATTCCTCAAAGACAAACTAAAGCCAGGTGCCGCA GAGGATGACCTTGTTTTAGAAGTGGTTATCATGATTGGGACGGTGTCTATGGATGACTCTTGTGCTGCCC TACTGGCCAAATCTGGGATAATCCCAGCCCTCATCGAGCTGCTGAACGCTCAACAAGAAGATGATGAATT TGTGTGTCAAATAATCTATGTCTTCTACCAGATGGTGTTCCATCAGGCCACAAGAGATGTCATAATCAAG GAAACACAAGCTCCAGCATATCTCATTGACCTGATGCATGATAAAAATAATGAAATCCGGAAAGTCTGTG ATAACACATTAGATATCATCGCAGAGTATGATGAAGAGTGGGCCAAGAAAATTCAGAGTGAGAAGTTTCG CTGGCATAACTCTCAGTGGCTGGAGATGGTGGAGAGCCGTCAGCTGGATGAGAGCGAGCAGTACTTGTAT GGTGATGATCGCATTGAGCCGTACATCCATGAAGGGGACATTCTTGAAAGGCCTGACCTTTTCTACAACT CAGACGGACTAATTACCTCTGAAGGAGCCATAAGTCCAGACTTCTTCAATGATTTTCACCTCCAGAATGG AGATGTGGTGGGGCAACACGCATTTCCTGGCAGCCTCGGGATGGATGGCTTCGGGCAGCCACTGGGGATC CTTGGACGTCCTGCCACCGCGTATGGATTCCGCCCTGACGAACCTTACTACTACAGCTTTGGGTCTCGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210688 representing BC049100
Red=Cloning site Green=Tags(s) MQGEDARYLKRKVKGGNIDVHPSEKALIVQYEVEATILGEMGDPMLGERKECQKIIRLKSLNANTDITSL ARKVVEECKLIHPSKLSEVEQLLYYLQNRRDSLPGKEKKEKSSKPKDPPPFEGMEIDEVANINDMDEYIE LLYEDIPDKVRGSALILQLARNPDNLEELLLNETALGALARVLREDWKQSVELATNIIYIFFCFSSFSHF HGLITHYKIGALCMNIIDHELKRHELWQEELSKKKKAVDEDLENQTLRKDYDKTFKKYQGLVVKQEQLLR VALYLLLNLAEDTRTELKMRNKNIVHMLVKALDRDNFELLILVVSFLKKLSIFMENKNDMVEMDIVEKLV KMIPCEHEDLLNITLRLLLNLSFDTGLRNKMVQVGLLPKLTALLGNENYKQIAMCVLYHISMDDRFKSMF AYTDCIPQLMKMLFECSDERIDLELISFCINLAANKRNVQLICEGNGLKMLMKRALKLKDPLLMKMIRNI SQHDGPTKNLFIDYVGDLAAQISSDEEEEFVIECLGTLANLTIPDLDWELVLKEYKLVPFLKDKLKPGAA EDDLVLEVVIMIGTVSMDDSCAALLAKSGIIPALIELLNAQQEDDEFVCQIIYVFYQMVFHQATRDVIIK ETQAPAYLIDLMHDKNNEIRKVCDNTLDIIAEYDEEWAKKIQSEKFRWHNSQWLEMVESRQLDESEQYLY GDDRIEPYIHEGDILERPDLFYNSDGLITSEGAISPDFFNDFHLQNGDVVGQHAFPGSLGMDGFGQPLGI LGRPATAYGFRPDEPYYYSFGSR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC049100 |
| ORF Size | 2379 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | BC049100.1 |
| RefSeq Size | 3952 bp |
| RefSeq ORF | 2381 bp |
| Locus ID | 16579 |
| MW | 144.9 kDa |
| Gene Summary | The protein encoded by this gene is the non-motor subunit of kinesin-2 complex, and forms a heterotrimer with two members of the kinesin superfamily of proteins that together form a microtubule plus-end directed translocator that plays an important role in intracellular transport, mitosis, and cell-cell adhesion. This protein contains multiple armadillo repeats involved in protein binding, and may serve as an adaptor to regulate binding of cargo with the motor proteins. Conditional disruption of this gene in mouse neural precursor cells caused a tumor-like phenotype and defective organization of the neuroepithelium thought to be the result of altered N-cadherin subcellular localization. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220731 | Kifap3 (untagged) - Mouse kinesin-associated protein 3 (cDNA clone MGC:61298 IMAGE:5698182), (10ug) |
CNY 9,120.00 |
|
| MG210688 | Kifap3 (tGFP-tagged) - Mouse kinesin-associated protein 3 (cDNA clone MGC:61298 IMAGE:5698182) |
CNY 5,040.00 |
|
| MR210688L3 | Lenti ORF clone of Kifap3 (Myc-DDK-tagged) - Mouse kinesin-associated protein 3 (cDNA clone MGC:61298 IMAGE:5698182) |
CNY 6,460.00 |
|
| MR210688L4 | Lenti ORF clone of Kifap3 (mGFP-tagged) - Mouse kinesin-associated protein 3 (cDNA clone MGC:61298 IMAGE:5698182) |
CNY 6,460.00 |
