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Hsp90b1 (NM_011631) Mouse Tagged ORF Clone
CAT＃: MR210732
- TrueORF®
Hsp90b1 (Myc-DDK-tagged) - Mouse heat shock protein 90, beta (Grp94), member 1 (Hsp90b1)
ORF Plasmid: tGFP
"NM_011631" in other vectors (4)
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CNY 5,880.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | endoplasmin; ERp99; gp96; GRP94; TA-3; Targ2; Tra-1; Tra1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210732 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGGTCCTGTGGGTGTTGGGCCTCTGCTGTGTCCTGCTGACCTTCGGGTTCGTCAGAGCTGATGATG AAGTCGACGTGGATGGCACAGTGGAAGAGGACCTGGGTAAAAGCCGAGAAGGCTCAAGGACAGATGATGA AGTTGTGCAGAGAGAGGAAGAAGCTATTCAGTTGGATGGGTTAAACGCATCACAGATAAGAGAACTTAGA GAAAAATCTGAAAAGTTCGCCTTCCAAGCTGAAGTGAACAGGATGATGAAACTTATCATCAATTCTTTGT ATAAAAATAAAGAGATTTTCCTGAGAGAACTGATTTCAAATGCTTCTGATGCTTTAGACAAGATAAGGCT CATCTCCCTAACTGATGAAAATGCACTCGCTGGAAATGAGGAGTTAACGGTCAAGATTAAGTGTGACAAA GAGAAAAACCTGCTGCATGTCACAGACACGGGTGTAGGAATGACTAGAGAGGAGTTGGTTAAAAATCTCG GCACCATAGCCAAATCTGGAACAAGCGAGTTTTTAAACAAAATGACAGAAGCTCAAGAAGATGGTCAGTC AACCTCTGAACTGATTGGCCAGTTTGGTGTCGGTTTTTATTCTGCCTTCCTTGTAGCAGATAAGGTCATT GTCACATCGAAACACAACAATGATACCCAGCACATCTGGGAATCAGACTCCAATGAATTCTCTGTAATTG CTGACCCAAGAGGAAACACACTAGGTCGTGGAACAACAATTACTCTTGTCTTAAAAGAAGAAGCATCTGA TTACCTTGAATTGGACACAATTAAAAATCTCGTCAGGAAGTACTCTCAGTTCATCAACTTTCCCATCTAC GTGTGGAGTAGCAAGACAGAGACTGTTGAGGAGCCCTTGGAAGAAGATGAAGCAGCAAAAGAAGAGAAAG AAGAATCTGATGATGAAGCTGCAGTAGAGGAGGAAGAAGAAGAAAAGAAACCAAAAACTAAGAAAGTTGA AAAAACTGTGTGGGATTGGGAACTTATGAATGATATCAAACCAATATGGCAGAGACCATCCAAAGAAGTA GAAGAAGACGAATACAAAGCTTTCTACAAATCATTTTCAAAGGAAAGTGATGACCCCATGGCTTATATCC ACTTCACTGCAGAAGGGGAGGTCACCTTCAAGTCGATTTTGTTTGTACCCACATCTGCACCTCGAGGTCT GTTTGATGAATATGGATCTAAGAAGAGTGATTATATTAAGCTGTATGTACGCCGCGTATTCATCACAGAT GACTTCCATGATATGATGCCCAAATACCTTAATTTTGTCAAAGGTGTTGTGGATTCCGATGATCTCCCCC TCAATGTTTCCCGTGAGACTCTTCAGCAACATAAATTGCTCAAGGTGATTAGGAAGAAGCTTGTCCGAAA AACTCTGGACATGATCAAGAAGATTGCTGATGAGAAGTATAACGACACTTTCTGGAAGGAGTTCGGCACG AATATCAAGCTTGGTGTGATTGAAGACCACTCAAATCGAACACGGCTTGCTAAACTTCTTAGGTTCCAGT CTTCTCACCATTCAACTGACATTACTAGTTTAGACCAGTATGTGGAAAGAATGAAGGAAAAACAGGACAA AATCTACTTCATGGCTGGGTCAAGCAGAAAGGAGGCGGAATCTTCTCCATTTGTTGAGAGACTTCTGAAG AAGGGCTATGAAGTCATTTATCTCACAGAGCCTGTGGATGAATACTGCATTCAGGCTCTTCCCGAGTTTG ATGGGAAGAGGTTTCAGAATGTTGCCAAAGAAGGGGTGAAGTTTGATGAGAGTGAGAAAACTAAAGAAAG TCGGGAAGCAACAGAGAAGGAGTTTGAACCTCTGCTCAACTGGATGAAAGATAAGGCCCTCAAGGACAAG ATAGAAAAGGCTGTGGTGTCGCAGCGCCTCACAGAGTCTCCCTGTGCTCTTGTGGCCAGTCAGTATGGAT GGTCTGGCAACATGGAGAGGATCATGAAGGCACAAGCATACCAGACGGGCAAGGACATCTCTACAAATTA CTATGCCAGTCAAAAGAAAACGTTCGAAATCAATCCTAGACACCCACTGATCAGAGACATGTTGCGGCGG ATTAAGGAAGATGAAGATGACAAGACAGTCATGGATCTTGCTGTAGTTTTGTTTGAAACGGCAACACTTC GGTCAGGATATCTTCTACCAGACACCAAGGCGTATGGAGATAGAATAGAAAGAATGCTTCGCCTCAGTTT AAACATTGACCCTGAAGCACAGGTGGAGGAAGAACCAGAAGAAGAGCCTGAAGACACCTCAGAAGAGGCA GAAGACTCAGAGCAGGATGAGGGAGAAGAGATGGATGCAGGGACAGAAGAAGAAGAGGAGGAAACAGAAA AGGAATCTACAGAGAAGGATGAATTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210732 protein sequence
Red=Cloning site Green=Tags(s) MRVLWVLGLCCVLLTFGFVRADDEVDVDGTVEEDLGKSREGSRTDDEVVQREEEAIQLDGLNASQIRELR EKSEKFAFQAEVNRMMKLIINSLYKNKEIFLRELISNASDALDKIRLISLTDENALAGNEELTVKIKCDK EKNLLHVTDTGVGMTREELVKNLGTIAKSGTSEFLNKMTEAQEDGQSTSELIGQFGVGFYSAFLVADKVI VTSKHNNDTQHIWESDSNEFSVIADPRGNTLGRGTTITLVLKEEASDYLELDTIKNLVRKYSQFINFPIY VWSSKTETVEEPLEEDEAAKEEKEESDDEAAVEEEEEEKKPKTKKVEKTVWDWELMNDIKPIWQRPSKEV EEDEYKAFYKSFSKESDDPMAYIHFTAEGEVTFKSILFVPTSAPRGLFDEYGSKKSDYIKLYVRRVFITD DFHDMMPKYLNFVKGVVDSDDLPLNVSRETLQQHKLLKVIRKKLVRKTLDMIKKIADEKYNDTFWKEFGT NIKLGVIEDHSNRTRLAKLLRFQSSHHSTDITSLDQYVERMKEKQDKIYFMAGSSRKEAESSPFVERLLK KGYEVIYLTEPVDEYCIQALPEFDGKRFQNVAKEGVKFDESEKTKESREATEKEFEPLLNWMKDKALKDK IEKAVVSQRLTESPCALVASQYGWSGNMERIMKAQAYQTGKDISTNYYASQKKTFEINPRHPLIRDMLRR IKEDEDDKTVMDLAVVLFETATLRSGYLLPDTKAYGDRIERMLRLSLNIDPEAQVEEEPEEEPEDTSEEA EDSEQDEGEEMDAGTEEEEEETEKESTEKDEL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011631 |
| ORF Size | 2409 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_011631.1, NP_035761.1 |
| RefSeq Size | 2759 bp |
| RefSeq ORF | 2409 bp |
| Locus ID | 22027 |
| UniProt ID | P08113 |
| MW | 92.5 kDa |
| Gene Summary | Molecular chaperone that functions in the processing and transport of secreted proteins (PubMed:20865800). When associated with CNPY3, required for proper folding of Toll-like receptors (PubMed:20865800). Functions in endoplasmic reticulum associated degradation (ERAD) (By similarity). Has ATPase activity (PubMed:20865800).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200634 | Hsp90b1 (untagged) - Mouse heat shock protein 90, beta (Grp94), member 1 (Hsp90b1), (10ug) |
CNY 5,880.00 |
|
| MG210732 | Hsp90b1 (tGFP-tagged) - Mouse heat shock protein 90kDa beta (Grp94), member 1 (Hsp90b1) |
CNY 6,370.00 |
|
| MR210732L3 | Lenti ORF clone of Hsp90b1 (Myc-DDK-tagged) - Mouse heat shock protein 90, beta (Grp94), member 1 (Hsp90b1) |
CNY 7,700.00 |
|
| MR210732L4 | Lenti ORF clone of Hsp90b1 (mGFP-tagged) - Mouse heat shock protein 90, beta (Grp94), member 1 (Hsp90b1) |
CNY 7,700.00 |
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