Eps8 (NM_007945) Mouse Tagged ORF Clone
CAT#: MR210818
- TrueORF®
Eps8 (Myc-DDK-tagged) - Mouse epidermal growth factor receptor pathway substrate 8 (Eps8)
ORF Plasmid: tGFP
"NM_007945" in other vectors (3)
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CNY 6,024.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | AW261790 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR210818 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATGGTCATATGTCTAACCGCTCCAGTGGGTATGGAGTCTACCCTTCTCAACTGAATGGTTACGGAT CTTCACCACCCTATTCCCAGATGGACAGAGAACACAGCTCAAGAACAAGTGCAAAGGCCCTTTATGAACA AAGGAAGAACTATGCCCGAGACAGTGTCAGCAGTGTGTCGGACGTGTCCCAGTACCGCGTGGAACACTTG ACCACCTTCGTGCTGGATCGGAAAGATGCAATGATCACTGTCGAGGACGGAATAAGAAAGCTGAAGTTGC TGGATGCCAAGGGCAAAGTGTGGACTCAAGATATGATTCTCCAAGTGGATGACCGAGCTGTGAGCCTGAT TGACTTAGAGTCAAAGAATGAATTGGAGAATTTTCCTCTAAACACAATCTCGCATTGTCAAGCAGTGGTG CATGCATGCAGCTATGACTCCATTCTCGCCTTGGTATGCAAAGAGCCAACGCAGAGCAAGCCAGACCTTC ACCTTTTCCAGTGTGATGAGGTTAAGGCAAACCTAATTAGTGAAGATATCGAAAGTGCAATCAGTGACAG TAAAGGTGGGAAACAGAAGAGGCGGCCGGAGGCCCTGAGGATGATTGCCAAAGCAGATCCTGGCATCCCT CCTCCTCCCAGAGCTCCTGCCCCTGTGCCACCGGGGACTGTCACACAGGTGGACGTTAGGAGTCGCGTAG CAGCCTGGTCTGCCTGGGCAGCTGACCAGGGTGACTTCGAGAAGCCCCGGCAGTACCACGAGCAAGAAGA GACGCCCGAGATGATGGCAGCCCGGATCGACAGGGATGTGCAAATCTTAAACCATATTTTGGATGACATT GAATTTTTTATCACCAAACTCCAAAAAGCCGCCGAAGCGTTTTCTGAGCTTTCTAAAAGGAAGAAAAGTA AGAAAAGTAAAAGGAAAGGACCTGGAGAGGGCGTTTTAACACTGAGGGCAAAACCGCCACCTCCTGACGA GTTTGTTGACTGTTTCCAGAAGTTTAAACATGGATTCAACCTTCTGGCCAAGTTGAAGTCCCATATCCAG AACCCGAGTGCTTCAGATCTGGTTCATTTTTTGTTTACTCCACTAAATATGGTGGTCCAGGCAACAGGTG GCCCTGAACTGGCCAGTTCGGTACTCAGCCCACTGTTGACAAAAGACACAGTTGATTTCTTAAACTACAC AGCCACTGCGGAGGAACGGAAGCTGTGGATGTCACTGGGAGATAGTTGGGTGAAAGTGAGAGCAGAGTGG CCGAAAGAACAGTTCATCCCACCTTACGTCCCGAGGTTCCGCAACGGCTGGGAGCCCCCGATGCTGAACT TCATGGGCGCGCCCACAGAGCAAGACATGTATCAACTGGCCGAGTCCGTGGCCAACGCAGAACACCAGCG CAAACAGGACAGCAAGAGGCTGTCCACAGAGCATTCCAATGTGTCCGACTATCCTCCAGCCGACGGATAT GCGTACAGTAGCAGCATGTACCACAGAGGACCACATGCAGACCACGGGGAGGCTGCCATGCCTTTCAAGT CAACTCCTAATCACCAAGTAGATAGGAATTATGACGCAGTCAAAACACAACCCAAGAAATACGCCAAATC CAAGTACGACTTTGTGGCGAGGAACAGCAGCGAGCTCTCGGTTATGAAAGATGATGTCTTAGAGATACTC GACGATCGAAGGCAGTGGTGGAAAGTCCGGAATGCCAGTGGAGACTCTGGGTTTGTGCCAAATAACATTC TGGATATCATGAGAACTCCAGAATCTGGAGTGGGGCGCGCTGACCCCCCATACACACATACCATACAGAA ACAAAGGACGGAATACGGCCTGAGATCAGCTGACACTCCTTCTGCCCCATCACCCCCTCCAACGCCAGCA CCCGTTCCGGTCCCCCTTCCACCTTCTGTACCAGCACCCGTTTCTGTGCCCAAGGTTCCAGCAGATGTCA CCCGCCAGAACAGCAGCTCCAGTGACAGTGGGGGCAGCATTGTGCGGGACAGCCAGAGATACAAACAACT CCCAGTGGACCGAAGGAAGTCCCAGATGGAAGAGGTTCAGGATGAGCTCTTCCAGAGGCTGACCATCGGG CGCAGTGCTGCGCAGAGGAAGTTCCACGTGCCACGGCAGAACGTTCCAGTGATCAATATCACTTATGACT CCTCACCGGAAGAAGTAAAGACTTGGCTGCAGTCAAAGGGATTCAATCCCGTGACTGTCAATAGCCTCGG GGTGTTGAACGGAGCACAACTCTTTTCTCTCAACAAAGACGAACTGAGGTCTGTCTGCCCGGAAGGTGCC AGAGTCTTTAACCAAATCACTGTTCAGAAAGCTGCTTTGGAGGACAGTAATGGAAGCTCCGAGTTACAAG AGATCATGCGGAGACGGCAGGAGAAGATCAGCGCCGCTGCGAGCGACTCGGGAGTGGAGTCTTTTGATGA AGGGAGCAGCCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210818 protein sequence
Red=Cloning site Green=Tags(s) MNGHMSNRSSGYGVYPSQLNGYGSSPPYSQMDREHSSRTSAKALYEQRKNYARDSVSSVSDVSQYRVEHL TTFVLDRKDAMITVEDGIRKLKLLDAKGKVWTQDMILQVDDRAVSLIDLESKNELENFPLNTISHCQAVV HACSYDSILALVCKEPTQSKPDLHLFQCDEVKANLISEDIESAISDSKGGKQKRRPEALRMIAKADPGIP PPPRAPAPVPPGTVTQVDVRSRVAAWSAWAADQGDFEKPRQYHEQEETPEMMAARIDRDVQILNHILDDI EFFITKLQKAAEAFSELSKRKKSKKSKRKGPGEGVLTLRAKPPPPDEFVDCFQKFKHGFNLLAKLKSHIQ NPSASDLVHFLFTPLNMVVQATGGPELASSVLSPLLTKDTVDFLNYTATAEERKLWMSLGDSWVKVRAEW PKEQFIPPYVPRFRNGWEPPMLNFMGAPTEQDMYQLAESVANAEHQRKQDSKRLSTEHSNVSDYPPADGY AYSSSMYHRGPHADHGEAAMPFKSTPNHQVDRNYDAVKTQPKKYAKSKYDFVARNSSELSVMKDDVLEIL DDRRQWWKVRNASGDSGFVPNNILDIMRTPESGVGRADPPYTHTIQKQRTEYGLRSADTPSAPSPPPTPA PVPVPLPPSVPAPVSVPKVPADVTRQNSSSSDSGGSIVRDSQRYKQLPVDRRKSQMEEVQDELFQRLTIG RSAAQRKFHVPRQNVPVINITYDSSPEEVKTWLQSKGFNPVTVNSLGVLNGAQLFSLNKDELRSVCPEGA RVFNQITVQKAALEDSNGSSELQEIMRRRQEKISAAASDSGVESFDEGSSH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_007945 |
ORF Size | 2466 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_007945.1 |
RefSeq Size | 4567 bp |
RefSeq ORF | 2466 bp |
Locus ID | 13860 |
UniProt ID | Q08509 |
MW | 91.7 kDa |
Gene Summary | Signaling adapter that controls various cellular protrusions by regulating actin cytoskeleton dynamics and architecture. Depending on its association with other signal transducers, can regulate different processes. Together with SOS1 and ABI1, forms a trimeric complex that participates in transduction of signals from Ras to Rac by activating the Rac-specific guanine nucleotide exchange factor (GEF) activity. Acts as a direct regulator of actin dynamics by binding actin filaments and has both barbed-end actin filament capping and actin bundling activities depending on the context. Displays barbed-end actin capping activity when associated with ABI1, thereby regulating actin-based motility process: capping activity is auto-inhibited and inhibition is relieved upon ABI1 interaction. Also shows actin bundling activity when associated with BAIAP2, enhancing BAIAP2-dependent membrane extensions and promoting filopodial protrusions. Involved in the regulation of processes such as axonal filopodia growth, stereocilia length, dendritic cell migration and cancer cell migration and invasion. Acts as a regulator of axonal filopodia formation in neurons: in the absence of neurotrophic factors, negatively regulates axonal filopodia formation via actin-capping activity. In contrast, it is phosphorylated in the presence of BDNF leading to inhibition of its actin-capping activity and stimulation of filopodia formation. Component of a complex with WHRN and MYO15A that localizes at stereocilia tips and is required for elongation of the stereocilia actin core. Indirectly involved in cell cycle progression; its degradation following ubiquitination being required during G2 phase to promote cell shape changes.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG210818 | Eps8 (tGFP-tagged) - Mouse epidermal growth factor receptor pathway substrate 8 (Eps8) |
CNY 6,370.00 |
|
MR210818L3 | Lenti ORF clone of Eps8 (Myc-DDK-tagged) - Mouse epidermal growth factor receptor pathway substrate 8 (Eps8) |
CNY 7,700.00 |
|
MR210818L4 | Lenti ORF clone of Eps8 (mGFP-tagged) - Mouse epidermal growth factor receptor pathway substrate 8 (Eps8) |
CNY 7,700.00 |