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Adam17 (NM_009615) Mouse Tagged ORF Clone
CAT＃: MR210844
- TrueORF®
Adam17 (Myc-DDK-tagged) - Mouse a disintegrin and metallopeptidase domain 17 (Adam17)
ORF Plasmid: tGFP
"NM_009615" in other vectors (4)
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CNY 6,064.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CD156b; Tac; Tace |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210844 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGCGGCGTCTCCTCATCCTGACCACTTTGGTGCCTTTCGTCCTGGCACCCCGACCTCCGGAGGAAG CAGGCTCTGGCTCCCATCCGCGACTTGAGAAGCTTGATTCTTTGCTCTCAGACTACGACATCCTCTCCTT AGCTAATATTCAGCAGCACTCCATAAGGAAAAGGGATCTACAGTCTGCGACACACTTAGAAACATTACTA ACTTTTTCAGCTTTGAAAAGACATTTTAAATTATACTTGACATCAAGTACCGAACGCTTTTCACAAAACT TGAGAGTCGTGGTGGTGGACGGGAAAGAAGAAAGCGAGTACAGCGTGAAGTGGCAGAACTTCTTCAGTGG TCACGTGGTTGGTGAGCCTGACTCTAGGGTTCTAGCCCACATAGGAGATGATGATGTTACAGTGAGAATC AACACAGATGGGGCAGAATATAACGTAGAGCCACTTTGGAGGTTTGTCAATGATACTAAAGATAAACGAA TGCTGGTGTATAAGTCTGAAGATATCAAGGATTTTTCACGTTTGCAGTCTCCAAAAGTATGTGGTTATTT AAATGCAGATAGTGAAGAGCTGCTTCCAAAAGGGCTCATAGACAGAGAGCCATCTGAAGAGTTTGTTCGT CGAGTGAAGAGACGAGCTGAACCTAACCCCTTGAAGAATACTTGTAAATTACTGGTGGTAGCAGATCATC GATTTTATAAATACATGGGCCGTGGAGAAGAGAGCACCACTACAAATTACTTAATAGAGCTAATTGACCG AGTTGATGACATATACCGGAACACGTCGTGGGATAATGCAGGGTTTAAAGGGTATGGAGTGCAGATAGAG CAGATTCGAATTCTCAAGTCTCCACAAGAGGTAAAACCTGGTGAAAGACACTTCAATATGGCAAAAAGTT TCCCAAACGAAGAGAAGGATGCTTGGGATGTGAAGATGCTATTAGAGCAATTTAGCTTTGATATAGCTGA AGAAGCATCTAAAGTCTGCCTGGCTCATCTTTTCACGTACCAGGATTTTGATATGGGAACTCTTGGATTA GCTTACGTTGGTTCTCCCAGAGCAAACAGTCATGGAGGGGTTTGTCCAAAAGCTTATTACAACCCAACTG TGAAGAAAAACATCTATTTAAATAGTGGTCTGACTAGTACTAAAAATTATGGCAAAACTATTCTCACAAA GGAAGCTGACCTGGTTACAACTCATGAATTGGGACATAATTTTGGAGCAGAACATGACCCTGATGGGCTA GCAGAATGTGCCCCAAATGAGGACCAAGGAGGAAAGTATGTCATGTATCCCATAGCTGTGAGCGGTGACC ACGAGAATAATAAGATGTTTTCAAACTGCAGTAAACAGTCCATCTACAAGACCATAGAAAGTAAGGCTCA AGAGTGCTTCCAGGAGCGCAGCAACAAGGTGTGTGGCAACTCCAGGGTGGATGAAGGAGAGGAGTGTGAC CCGGGTATTATGTACCTGAACAACGACACCTGCTGCAATAGTGACTGCACACTGAAGCCGGGTGTGCAGT GCAGTGATAGGAACAGTCCTTGCTGTAAAAACTGTCAGTTTGAGACGGCGCAGAAGAAGTGCCAGGAGGC TATTAATGCTACATGCAAAGGAGTGTCTTACTGCACAGGGAATAGCAGTGAGTGCCCCCCACCCGGAGAT GCTGAAGATGACACTGTGTGCTTGGACCTTGGCAAGTGCAAGGCTGGGAAATGCATCCCTTTCTGCAAGA GGGAGCAGGAGCTGGAGTCCTGCGCATGCGTTGACACTGACAACTCGTGCAAGGTGTGCTGCAGGAACCT TTCTGGCCCGTGTGTGCCGTACGTCGATGCAGAGCAAAAGAACTTGTTTTTGAGGAAAGGGAAGCCATGT ACAGTAGGGTTTTGCGACATGAATGGCAAATGTGAGAAACGAGTACAGGACGTAATTGAGCGATTTTGGG ATTTCATTGACCAGCTGAGCATCAACACTTTTGGGAAGTTTCTGGCAGATAACATCGTTGGGTCTGTTCT GGTTTTCTCCTTGATATTTTGGATTCCTTTCAGCATTCTTGTCCACTGTGTGGATAAGAAACTGGACAAG CAGTATGAATCCCTGTCTCTGTTTCATCACAGTAACATTGAGATGCTGAGCAGCATGGACTCAGCATCTG TTCGCATCATCAAGCCCTTTCCTGCACCCCAGACTCCAGGTCGTCTGCAGGCCCTGCAGCCAGCTGCCAT GATGCCGCCAGTACCTGCAGCTCCAAAACTGGACCACCAGAGGATGGATACCATCCAGGAAGACCCCAGC ACAGACTCACATGCAGATGATGACGGTTTTGAGAAGGACCCCTTCCCCAACAGCAGCACAGCTGCCAAGT CCTTTGAGGATCTCACAGACCACCCAGTCACCAGGAGCGAAAAGGCGGCCTCATTCAAGCTGCAGCGTCA GAGCCGAGTTGACAGCAAAGAGACAGAGTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210844 protein sequence
Red=Cloning site Green=Tags(s) MRRRLLILTTLVPFVLAPRPPEEAGSGSHPRLEKLDSLLSDYDILSLANIQQHSIRKRDLQSATHLETLL TFSALKRHFKLYLTSSTERFSQNLRVVVVDGKEESEYSVKWQNFFSGHVVGEPDSRVLAHIGDDDVTVRI NTDGAEYNVEPLWRFVNDTKDKRMLVYKSEDIKDFSRLQSPKVCGYLNADSEELLPKGLIDREPSEEFVR RVKRRAEPNPLKNTCKLLVVADHRFYKYMGRGEESTTTNYLIELIDRVDDIYRNTSWDNAGFKGYGVQIE QIRILKSPQEVKPGERHFNMAKSFPNEEKDAWDVKMLLEQFSFDIAEEASKVCLAHLFTYQDFDMGTLGL AYVGSPRANSHGGVCPKAYYNPTVKKNIYLNSGLTSTKNYGKTILTKEADLVTTHELGHNFGAEHDPDGL AECAPNEDQGGKYVMYPIAVSGDHENNKMFSNCSKQSIYKTIESKAQECFQERSNKVCGNSRVDEGEECD PGIMYLNNDTCCNSDCTLKPGVQCSDRNSPCCKNCQFETAQKKCQEAINATCKGVSYCTGNSSECPPPGD AEDDTVCLDLGKCKAGKCIPFCKREQELESCACVDTDNSCKVCCRNLSGPCVPYVDAEQKNLFLRKGKPC TVGFCDMNGKCEKRVQDVIERFWDFIDQLSINTFGKFLADNIVGSVLVFSLIFWIPFSILVHCVDKKLDK QYESLSLFHHSNIEMLSSMDSASVRIIKPFPAPQTPGRLQALQPAAMMPPVPAAPKLDHQRMDTIQEDPS TDSHADDDGFEKDPFPNSSTAAKSFEDLTDHPVTRSEKAASFKLQRQSRVDSKETEC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009615 |
| ORF Size | 2484 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_009615.2, NM_009615.3, NM_009615.5, NM_009615.6, NP_033745.4 |
| RefSeq Size | 4451 bp |
| RefSeq ORF | 2484 bp |
| Locus ID | 11491 |
| UniProt ID | Q9Z0F8 |
| MW | 93.1 kDa |
| Gene Summary | This gene encodes a member of a disintegrin and metalloprotease (ADAM) family of endoproteases that play important roles in various biological processes including cell signaling, adhesion and migration. The encoded preproprotein undergoes proteolytic processing to generate a mature enzyme that is involved in the proteolytic release of membrane-bound proteins in a process called ectodomain shedding. Mice lacking the encoded protein die in utero or fail to survive beyond one week of age. Alternative splicing results in multiple transcript variants encoding different isoforms, some of which may undergo similar processing. [provided by RefSeq, May 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221841 | Adam17 (untagged) - Mouse a disintegrin and metallopeptidase domain 17 (Adam17), (10ug) |
CNY 6,064.00 |
|
| MG210844 | Adam17 (tGFP-tagged) - Mouse a disintegrin and metallopeptidase domain 17 (Adam17) |
CNY 6,370.00 |
|
| MR210844L3 | Lenti ORF clone of Adam17 (Myc-DDK-tagged) - Mouse a disintegrin and metallopeptidase domain 17 (Adam17) |
CNY 7,700.00 |
|
| MR210844L4 | Lenti ORF clone of Adam17 (mGFP-tagged) - Mouse a disintegrin and metallopeptidase domain 17 (Adam17) |
CNY 7,700.00 |
