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Pik3c3 (BC024675) Mouse Tagged ORF Clone
CAT＃: MR210937
- TrueORF®
Pik3c3 (Myc-DDK-tagged) - Mouse phosphoinositide-3-kinase, class 3 (cDNA clone MGC:30377 IMAGE:3661094)
ORF Plasmid: tGFP
"BC024675" in other vectors (4)
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CNY 6,208.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 5330434F23, Vps34 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210937 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGGAGGCGGAGAAGTTCCACTACATCTACAGCTGTGACCTGGACATCAACGTGCAGCTGAAGATAG GGAGCTTGGAAGGGAAGAGAGAACAAAAGAGCTATAAAGCTGTCCTAGAAGATCCCATGTTAAAGTTTTC TGGGCTATACCAAGAGACATGCTCCGACCTCTATGTGACTTGTCAAGTGTTTGCTGAAGGGAAGCCCCTG GCCTTACCAGTCAGAACATCCTACAAGGCGTTTAGTACAAGATGGAATTGGAACGAGTGGCTGAAACTTC CTGTCAAGTACCCTGACCTGCCCAGGAATGCCCAAGTGGCCTTGACTATATGGGATGTGTATGGACCGGG AAGCGCTGTGCCAGTGGGAGGAACAACCGTGTCGCTCTTTGGAAAATATGGCATGTTTCGCCAAGGAATG CATGACTTGAAGGTCTGGCCTAACGTGGAGGCAGATGGTTCTGAACCTACAAGAACTCCCGGCAGAACAA GCAGCACACTGTCAGAAGATCAGATGAGCCGCCTCGCCAAGCTCACCAAGGCTCATCGGCAAGGACACAT GGTGAAAGTGGATTGGCTGGACAGATTAACATTTAGAGAGATAGAAATGATAAATGAGAGTGAGAAACGA AGCTCTAATTTCATGTACTTGATGGTTGAGTTTCGCTGTGTAAAGTGCGATGACAAGGAGTATGGCATTG TTTACTATGAAAAGGATGGTGACGAATCATCTCCAATTTTAACCAGTTTTGAGTTAGTGAAAGTTCCTGA TCCTCAAATGTCTATGGAGAATTTAGTGGAGAGCAAACACCACAAGCTTGCTCGGAGCTTAAGAAGCGGA CCATCTGACCACGATCTCAAACCCAACGCTACCACAAGAGATCAGCTAAATATTATTGTGAGTTACCCAC CAACCAAGCAACTCACATATGAAGAACAAGATCTTGTTTGGAAATTTAGATATTATCTTACTAATCAAGA AAAAGCTCTGACGAAGTTCTTGAAGTGTGTTAATTGGGACCTGCCCCAGGAGGCCAAGCAGGCCCTGGAA CTTCTGGGAAAGTGGAAGCCAATGGATGTAGAGGACTCCCTGGAGCTGCTCTCCTCTCATTACACCAACC CCACCGTGAGGCGCTACGCTGTGGCCCGCCTGAGGCAGGCCGATGATGAGGATTTGCTGATGTACTTACT GCAGCTGGTCCAGGCTCTGAAATATGAAAACTTTGATGACATAAAGAATGGTTTGGAACCTACGAAGAAG GATAGTCAAACTTCAGCATCAGAAAGTCTGTCCAATTCTGGAGTCAGTTCTGGAGACATAGATAGCTCCC AAATTATAACCAACCCTCTTCCTCCCGTGGCCCCCCCTCCTGCATCTAAAGCAAAGGAAGTTTCGGATGG GGAGAGTCTCGAGCAAGATCTATGTACGTTCTTGATATCAAGAGCCTGTAAGAACTCAACACTGGCTAAT TATTTATACTGGTATGTGATAGTGGAGTGTGAAGATCAGGACACCCAGCAGCGGGACCCAAAGACTCACG AGATGTACCTGAACGTGATGAGGCGCTTCAGCCAAGCCTTGCTCAAGGGTGACAAGTCTGTGAGAGTCAT GCGCTCCCTGCTGGCTGCTCAGCAGACCTTTGTAGATCGCCTGGTCCATCTGATGAAGGCAGTGCAGAGA GAAAGTGGGAACCGCAAGAAGAAGAATGAGAGACTTCAGGCCTTGCTTGGCGATAATGAGAAAATGAACT TATCAGATGTGGAACTGATCCCATTGCCGCTGGAGCCACAGGTGAAAATAAGGGGCATCATCCCGGAAAC AGCTACCCTGTTCAAGAGTGCTCTTATGCCTGCCCAGCTGTTCTTCAAGACTGAAGATGGAGGCAAATAC CCAGTTATTTTCAAGCATGGAGACGACTTGCGTCAAGATCAGCTTATTCTTCAGATCATCTCCCTCATGG ACAAGCTGTTACGGAAAGAAAACCTGGATTTGAAATTGACCCCATATAAGGTGTTAGCCACTAGCACAAA ACATGGCTTCATGCAGTTCATCCAGTCGGTTCCTGTCGCAGAAGTTCTTGACACAGAGGGAAGCATTCAG AACTTTTTCAGGAAATATGCCCCCAGTGAGACTGGACCCTATGGCATCAGTGCAGAGGTCATGGACACTT ACGTTAAAAGCTGTGCTGGATACTGTGTGATTACGTACATTCTTGGAGTTGGAGACCGGCACCTGGATAA CCTTTTGTTAACAAAGACAGGCAAACTCTTCCATATAGATTTCGGGTATATTTTGGGTCGAGATCCGAAG CCCCTCCCTCCTCCGATGAAGCTGAACAAGGAGATGGTGGAAGGGATGGGTGGCACCCAGAGTGAGCAGT ACCAAGAGTTCCGAAAGCAGTGCTACACAGCCTTCCTCCACCTGCGAAGGTATTCCAATCTGATCTTGAA CTTGTTCTCCTTGATGGTTGATGCAAACATTCCAGATATTGCTCTTGAACCAGATAAAACTGTAAAAAAG ATATCCTGCCACAGAAAAATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210937 protein sequence
Red=Cloning site Green=Tags(s) MGEAEKFHYIYSCDLDINVQLKIGSLEGKREQKSYKAVLEDPMLKFSGLYQETCSDLYVTCQVFAEGKPL ALPVRTSYKAFSTRWNWNEWLKLPVKYPDLPRNAQVALTIWDVYGPGSAVPVGGTTVSLFGKYGMFRQGM HDLKVWPNVEADGSEPTRTPGRTSSTLSEDQMSRLAKLTKAHRQGHMVKVDWLDRLTFREIEMINESEKR SSNFMYLMVEFRCVKCDDKEYGIVYYEKDGDESSPILTSFELVKVPDPQMSMENLVESKHHKLARSLRSG PSDHDLKPNATTRDQLNIIVSYPPTKQLTYEEQDLVWKFRYYLTNQEKALTKFLKCVNWDLPQEAKQALE LLGKWKPMDVEDSLELLSSHYTNPTVRRYAVARLRQADDEDLLMYLLQLVQALKYENFDDIKNGLEPTKK DSQTSASESLSNSGVSSGDIDSSQIITNPLPPVAPPPASKAKEVSDGESLEQDLCTFLISRACKNSTLAN YLYWYVIVECEDQDTQQRDPKTHEMYLNVMRRFSQALLKGDKSVRVMRSLLAAQQTFVDRLVHLMKAVQR ESGNRKKKNERLQALLGDNEKMNLSDVELIPLPLEPQVKIRGIIPETATLFKSALMPAQLFFKTEDGGKY PVIFKHGDDLRQDQLILQIISLMDKLLRKENLDLKLTPYKVLATSTKHGFMQFIQSVPVAEVLDTEGSIQ NFFRKYAPSETGPYGISAEVMDTYVKSCAGYCVITYILGVGDRHLDNLLLTKTGKLFHIDFGYILGRDPK PLPPPMKLNKEMVEGMGGTQSEQYQEFRKQCYTAFLHLRRYSNLILNLFSLMVDANIPDIALEPDKTVKK ISCHRKM TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC024675 |
| ORF Size | 2541 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC024675, AAH24675 |
| RefSeq Size | 3018 bp |
| RefSeq ORF | 2543 bp |
| Locus ID | 225326 |
| MW | 96.8 kDa |
| Gene Summary | Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for transport from early to late endosomes (By similarity).[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Beclin-1 knockdown shows abscission failure but not autophagy defect during oocyte meiotic maturation
,You, SY;Park, YS;Jeon, HJ;Cho, DH;Jeon, HB;Kim, SH;Chang, JW;Kim, JS;Oh, JS;,
Cell Cycle
,PubMed ID 27149384
[Pik3c3]
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Documents
| Product Manuals |
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220903 | Pik3c3 (untagged) - Mouse phosphoinositide-3-kinase, class 3 (cDNA clone MGC:30377 IMAGE:3661094), (10ug) |
CNY 9,120.00 |
|
| MG210937 | Pik3c3 (tGFP-tagged) - Mouse phosphoinositide-3-kinase, class 3 (cDNA clone MGC:30377 IMAGE:3661094) |
CNY 7,808.00 |
|
| MR210937L3 | Lenti ORF clone of Pik3c3 (Myc-DDK-tagged) - Mouse phosphoinositide-3-kinase, class 3 (cDNA clone MGC:30377 IMAGE:3661094) |
CNY 6,750.00 |
|
| MR210937L4 | Lenti ORF clone of Pik3c3 (mGFP-tagged) - Mouse phosphoinositide-3-kinase, class 3 (cDNA clone MGC:30377 IMAGE:3661094) |
CNY 6,750.00 |
