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Usp5 (NM_013700) Mouse Tagged ORF Clone
CAT＃: MR210971
- TrueORF®
Usp5 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 5 (isopeptidase T) (Usp5)
ORF Plasmid: tGFP
"NM_013700" in other vectors (4)
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CNY 6,288.00
CNY 7,510.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AA407472; ISOT; ISOT-1; Ucht |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210971 representing NM_013700
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAGCTGAGTGAAGAGGCGCTGCTGTCAGTGTTACCGACGATCCGTGTCCCCAAGGCGGGAGACC GGGTCCATAAAGACGAGTGCGCTTTCTCTTTCGACACGCCGGAGTCTGAGGGTGGCCTCTATATCTGCAT GAACACATTCCTGGGATTCGGGAAGCAGTATGTGGAGAGACACTTCAACAAGACAGGCCAGCGTGTCTAC CTGCACCTCCGGAGGACCCGGCGACCGAAAGAAGAGGACACCAGTGCAGGCACTGGAGACCCACCTCGGA AGAAGCCCACCCGGCTGGCCATTGGTGTTGAAGGAGGGTTTGACCTCACCGAGGACAAGTTTGAATTTGA CGAGGATGTGAAGATTGTCATTTTGCCCGATTACCTGGAGATCGCTCGGGATGGGTTGGGGGGGCTTCCT GACATTGTCAGAGATCGGGTGACCAGTGCTGTGGAAGCCCTACTTTCTGCTGACTCAGCCTCTCGAAAGC AGGAGGTCCAGGCTTGGGATGGGGAAGTCCGTCAGGTGTCCAAGCATGCCTTCAACCTCAAGCAGTTGGA CAACCCTGCTCGGATCCCTCCCTGTGGCTGGAAGTGCTCCAAGTGCGACATGCGCGAGAACCTGTGGCTC AACCTGACAGACGGCTCCATCCTCTGTGGACGGCGCTACTTCGATGGCAGTGGGGGCAACAACCATGCTG TGGAGCACTACAGGGAGACTGGCTACCCCTTAGCTGTTAAGCTGGGCACCATCACCCCGGATGGAGCTGA CGTGTATTCTTACGACGAGGATGACATGGTTCTGGACCCCAGCCTGGCAGAGCACTTGTCTCACTTTGGC ATCGACATGCTGAAGATGCAGAAGACGGACAAGACGATGACTGAGCTGGAGATAGACATGAACCAGCGGA TTGGTGAATGGGAGTTGATCCAGGAGTCAGGCGTGCCACTCAAGCCTCTGTTTGGGCCTGGTTACACGGG CATCCGGAACCTGGGTAACAGCTGCTATCTCAACTCCGTGGTCCAGGTGCTCTTCAGCATCCCTGACTTT CAGAGGAAGTATGTGGATAAATTGGAGAAGATCTTCCAGAATGCCCCAACGGACCCCACCCAAGACTTCA GCACCCAGGTGGCCAAGCTGGGGCATGGCCTTCTCTCTGGAGAATACTCTAAGCCAGCACTGGAGTCAGG TGATGGGGAGCAGGTACCAGAACAAAAGGAAGTTCAAGATGGCATTGCCCCTCGAATGTTCAAGGCCCTC ATTGGCAAGGGCCACCCTGAGTTCTCCACTAATCGGCAGCAGGATGCCCAGGAATTCTTCCTACATCTTA TCAACATGGTGGAGAGGAATTGCCGGAGTTCTGAAAATCCAAATGAAGTGTTCCGCTTTTTGGTGGAAGA AAAGATCAAATGCCTGGCCACAGAGAAGGTTAAGTACACTCAGCGAGTTGACTACATCATGCAGTTGCCT GTACCCATGGATGCAGCCCTTAACAAAGAGGAGCTTTTAGAGTATGAAGAGAAGAAGCGGCAAGCTGAAG AGGAGAAAGTGCCACTGCCAGAACTGGTTCGGGCCCAGGTGCCCTTCAGCTCCTGCCTGGAAGCCTACGG GGCCCCTGAGCAGGTGGACGACTTCTGGAGTACAGCTCTACAGGCGAAGTCGGTCGCTGTCAAGACCACG CGCTTTGCCTCCTTCCCCGACTACCTGGTCATCCAGATCAAGAAGTTCACCTTTGGCTTAGACTGGGTGC CCAAGAAGCTGGATGTGTCCATTGAGATGCCAGAGGAGCTCGATATCTCCCAGCTGAGGGGCACAGGGCT ACAGCCGGGAGAGGAGGAGCTGCCTGACATTGCCCCACCCCTGGTCACTCCGGATGAGCCCAAAGGTAGC CTTGGTTTCTATGGCAACGAAGACGAAGACTCCTTCTGCTCCCCTCACTTCTCCTCTCCGACATCACCCA TGCTGGACGAATCCGTCATCATACAGTTGGTGGAAATGGGCTTCCCTATGGATGCCTGCCGGAAAGCTGT GTACTATACGGGAAACAGCGGGGCCGAAGCAGCCATGAACTGGGTCATGTCACACATGGATGATCCAGAT TTTGCAAACCCCCTCATCCTGCCTGGCTCCAGTGGGCCTGGCTCCACAAGTGCAGCAGCTGACCCCCCGC CGGAGGACTGTGTGACCACCATAGTCTCCATGGGCTTCTCCAGGGACCAGGCCCTGAAAGCTCTGCGGGC CACGAACAATAGCTTAGAGCGGGCTGTAGACTGGATCTTCAGCCACATTGATGACCTGGACGCAGAAGCT GCTATGGACATCTCAGAGGGACGCTCAGCGGCTGAGTCCATCTCCGAGTCTGTGCCAGTGGGACCTAAAG TCCGGGATGGTCCTGGAAAGTATCAGCTCTTTGCCTTCATTAGTCACATGGGCACATCTACTATGTGTGG TCACTATGTCTGCCATATCAAGAAGGAAGGCAGGTGGGTGATCTACAATGACCAGAAAGTGTGTGCCTCT GAAAAGCCACCCAAGGACCTGGGCTACATCTACTTCTACCAGAGAGTGGTCAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210971 representing NM_013700
Red=Cloning site Green=Tags(s) MAELSEEALLSVLPTIRVPKAGDRVHKDECAFSFDTPESEGGLYICMNTFLGFGKQYVERHFNKTGQRVY LHLRRTRRPKEEDTSAGTGDPPRKKPTRLAIGVEGGFDLTEDKFEFDEDVKIVILPDYLEIARDGLGGLP DIVRDRVTSAVEALLSADSASRKQEVQAWDGEVRQVSKHAFNLKQLDNPARIPPCGWKCSKCDMRENLWL NLTDGSILCGRRYFDGSGGNNHAVEHYRETGYPLAVKLGTITPDGADVYSYDEDDMVLDPSLAEHLSHFG IDMLKMQKTDKTMTELEIDMNQRIGEWELIQESGVPLKPLFGPGYTGIRNLGNSCYLNSVVQVLFSIPDF QRKYVDKLEKIFQNAPTDPTQDFSTQVAKLGHGLLSGEYSKPALESGDGEQVPEQKEVQDGIAPRMFKAL IGKGHPEFSTNRQQDAQEFFLHLINMVERNCRSSENPNEVFRFLVEEKIKCLATEKVKYTQRVDYIMQLP VPMDAALNKEELLEYEEKKRQAEEEKVPLPELVRAQVPFSSCLEAYGAPEQVDDFWSTALQAKSVAVKTT RFASFPDYLVIQIKKFTFGLDWVPKKLDVSIEMPEELDISQLRGTGLQPGEEELPDIAPPLVTPDEPKGS LGFYGNEDEDSFCSPHFSSPTSPMLDESVIIQLVEMGFPMDACRKAVYYTGNSGAEAAMNWVMSHMDDPD FANPLILPGSSGPGSTSAAADPPPEDCVTTIVSMGFSRDQALKALRATNNSLERAVDWIFSHIDDLDAEA AMDISEGRSAAESISESVPVGPKVRDGPGKYQLFAFISHMGTSTMCGHYVCHIKKEGRWVIYNDQKVCAS EKPPKDLGYIYFYQRVVS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_013700 |
| ORF Size | 2574 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_013700.2, NP_038728.1 |
| RefSeq Size | 3176 bp |
| RefSeq ORF | 2577 bp |
| Locus ID | 22225 |
| UniProt ID | P56399 |
| MW | 96.3 kDa |
| Gene Summary | Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys-63'-linked polyubiquitin with a lower affinity (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222154 | Usp5 (untagged) - Mouse ubiquitin specific peptidase 5 (isopeptidase T) (Usp5), (10ug) |
CNY 10,930.00 |
|
| MG210971 | Usp5 (tGFP-tagged) - Mouse ubiquitin specific peptidase 5 (isopeptidase T) (Usp5) |
CNY 8,270.00 |
|
| MR210971L3 | Lenti ORF clone of Usp5 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 5 (isopeptidase T) (Usp5) |
CNY 9,410.00 |
|
| MR210971L4 | Lenti ORF clone of Usp5 (mGFP-tagged) - Mouse ubiquitin specific peptidase 5 (isopeptidase T) (Usp5) |
CNY 9,410.00 |
