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Sh3rf1 (BC060113) Mouse Tagged ORF Clone
CAT＃: MR210987
- TrueORF®
Sh3rf1 (Myc-DDK-tagged) - Mouse SH3 domain containing ring finger 1 (cDNA clone MGC:63290 IMAGE:6405370)
ORF Plasmid: tGFP
"BC060113" in other vectors (4)
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CNY 6,304.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2200003J05Rik; Posh; R75531; Sh3md2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210987 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGAGTCTGCCTTGTTGGACCTTCTGGAGTGCCCTGTGTGTCTAGAACGCCTGGATGCTTCCGCAA AGGTCTTACCCTGCCAGCATACCTTTTGCAAACGCTGTTTGCTGGGGATTGTGGGTTCCCGGAATGAACT CAGATGTCCCGAATGCCGGACTCTTGTTGGCTCTGGGGTCGACGAGCTCCCCAGTAACATCCTACTGGTC AGACTTCTGGATGGCATCAAGCAGAGGCCTTGGAAACCCGGCCCTGGTGGGGGCGGCGGGACCACCTGCA CAAACACATTAAGGGCGCAGGGCAGCACTGTGGTTAATTGTGGCTCGAAAGATCTGCAGAGCTCCCAGTG TGGACAGCAGCCTCGGGTGCAAGCCTGGAGCCCCCCAGTGAGGGGAATACCTCAGTTACCGTGTGCCAAA GCATTATATAACTACGAAGGAAAAGAGCCCGGAGACCTTAAGTTCAGCAAAGGCGACATCATCAAACCTT TACCTCAGCCCCCGCCTCAGTGCAAAGCACTTTACGACTTTGAAGTGAAAGACAAGGAAGCTGACAAAGA TTGCCTTCCCTTCGCAAAGGACGACGTACTGACCGTGATCCGCAGAGTGGATGAAAACTGGGCTGAAGGA ATGCTGGCAGATAAAATAGGAATATTTCCAATTTCATACGTGGAGTTTAACTCAGCTGCCAAGCAGCTGA TAGAGTGGGATAAGCCTCCCGTGCCAGGAGTGGACACGGCAGAATGCCCCTCAGCGACGGCGCAGAGCAC CTCTGCCTCAAAGCACCCCGACACCAAGAAGAACACCAGGAAGCGACACTCCTTCACCTCCCTCACCATG GCCAACAAGTCTTCCCAGGGGTCCCAGAACCGCCACTCCATGGAGATCAGCCCTCCTGTGCTCATCAGTT CCAGCAACCCCACAGCCGCAGCCCGCATCAGCGAACTGTCCGGGCTCTCCTGCAGCGCCCCGTCTCAGGT CCATATAAGCACCACTGGGTTAATTGTGACCCCACCCCCTAGCAGCCCGGTGACAACTGGCCCTGCGTTC ACGTTCCCTTCAGATGTCCCCTACCAAGCTGCCCTTGGAAGTATGAATCCTCCACTTCCCCCACCCCCTC TCCTGGCGGCCACCGTACTCGCCTCCACCCCGTCAGGCGCTACTGCTGCTGTTGCTGCTGCTGCTGCCGC CGCCGCTGCTGGAATGGGACCCAGGCCTGTGATGGGGTCCTCTGAACAGATTGCACATTTACGGCCTCAG ACTCGTCCCAGTGTATATGTTGCTATATATCCGTACACTCCCCGGAAGGAAGACGAACTGGAGCTGAGGA AAGGGGAGATGTTTTTGGTGTTTGAGCGTTGCCAGGACGGCTGGTACAAAGGGACATCGATGCATACCAG CAAGATAGGCGTTTTCCCTGGCAACTATGTGGCGCCCGTCACAAGGGCGGTGACGAATGCCTCCCAAGCT AAAGTCTCTATGTCTACTGCGGGTCAAGCAAGTCGCGGGGTGACCATGGTCAGCCCTTCCACTGCAGGAG GACCTACACAGAAGCCCCAAGGAAACGGCGTGGCCGGAAATCCCAGCGTCGTCCCCACGGCTGTGGTGTC AGCAGCTCATATCCAGACAAGTCCTCAGGCTAAGGTCCTGCTGCACATGTCTGGGCAGATGACAGTCAAT CAGGCCCGCAATGCTGTGAGGACAGTTGCAGCACATAGCCAGGAACGCCCCACGGCAGCAGTGACTCCCA TCCAGGTCCAGAATGCCGCCTGCCTCGGTCCTGCATCCGTGGGCCTGCCCCATCATTCTCTGGCCTCCCA ACCTCTGCCTCCAATGGCGGGTCCTGCTGCCCACGGTGCTGCCGTCAGCATCAGTCGAACCAATGCCCCC ATGGCCTGCGCTGCAGGGGCTTCTCTGGCCTCCCCAAATATGACCAGTGCCGTGTTGGAGACAGAGCCCA GTGGTCGCACAGTGACCATCCTCCCTGGACTCCCCACATCTCCAGAGAGTGCTGCATCAGCGTGTGGGAA CAGTTCAGCTGGGAAACCAGACAAGGACAGTAAGAAAGAAAAAAAGGGCCTACTGAAGCTGCTTTCTGGT GCCTCCACCAAACGCAAGCCCCGAGTCTCCCCTCCAGCATCACCTACCCTGGATGTGGAGCTGGGTGCTG GGGAGGCTCCCTTGCAGGGAGCAGTAGGTCCTGAGCTGCCGCTAGGGGGCAGCCACGGCAGAGTGGGGTC ATGCCCCACAGATGGTGATGGTCCAGTGGCCGCTGGAACAGCAGCCCTAGCCCAGGATGCCTTCCACCGC AAGACAAGCTCCCTGGACTCCGCAGTGCCCATTGCTCCACCACCTCGCCAGGCCTGCTCCTCCCTGGGCC CAGTCATGAATGAGGCCCGGCCTGTTGTTTGTGAAAGGCACAGGGTGGTGGTTTCCTACCCTCCTCAGAG TGAGGCCGAACTTGAACTCAAGGAAGGAGATATTGTGTTTGTTCATAAGAAACGAGAGGACGGCTGGTTC AAAGGCACGTTACAGAGGAATGGGAAGACTGGCCTTTTCCCAGGGAGCTTTGTGGAAAACATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210987 protein sequence
Red=Cloning site Green=Tags(s) MDESALLDLLECPVCLERLDASAKVLPCQHTFCKRCLLGIVGSRNELRCPECRTLVGSGVDELPSNILLV RLLDGIKQRPWKPGPGGGGGTTCTNTLRAQGSTVVNCGSKDLQSSQCGQQPRVQAWSPPVRGIPQLPCAK ALYNYEGKEPGDLKFSKGDIIKPLPQPPPQCKALYDFEVKDKEADKDCLPFAKDDVLTVIRRVDENWAEG MLADKIGIFPISYVEFNSAAKQLIEWDKPPVPGVDTAECPSATAQSTSASKHPDTKKNTRKRHSFTSLTM ANKSSQGSQNRHSMEISPPVLISSSNPTAAARISELSGLSCSAPSQVHISTTGLIVTPPPSSPVTTGPAF TFPSDVPYQAALGSMNPPLPPPPLLAATVLASTPSGATAAVAAAAAAAAAGMGPRPVMGSSEQIAHLRPQ TRPSVYVAIYPYTPRKEDELELRKGEMFLVFERCQDGWYKGTSMHTSKIGVFPGNYVAPVTRAVTNASQA KVSMSTAGQASRGVTMVSPSTAGGPTQKPQGNGVAGNPSVVPTAVVSAAHIQTSPQAKVLLHMSGQMTVN QARNAVRTVAAHSQERPTAAVTPIQVQNAACLGPASVGLPHHSLASQPLPPMAGPAAHGAAVSISRTNAP MACAAGASLASPNMTSAVLETEPSGRTVTILPGLPTSPESAASACGNSSAGKPDKDSKKEKKGLLKLLSG ASTKRKPRVSPPASPTLDVELGAGEAPLQGAVGPELPLGGSHGRVGSCPTDGDGPVAAGTAALAQDAFHR KTSSLDSAVPIAPPPRQACSSLGPVMNEARPVVCERHRVVVSYPPQSEAELELKEGDIVFVHKKREDGWF KGTLQRNGKTGLFPGSFVENI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC060113 |
| ORF Size | 2583 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC060113, AAH60113 |
| RefSeq Size | 5074 bp |
| RefSeq ORF | 2585 bp |
| Locus ID | 59009 |
| MW | 89.8 kDa |
| Gene Summary | Has E3 ubiquitin-protein ligase activity. In the absence of an external substrate, it can catalyze self-ubiquitination. Stimulates ubiquitination of potassium channel KCNJ1, enhancing its dynamin-dependent and clathrin-independent endocytosis (By similarity). Acts as a scaffold protein that coordinates with MAPK8IP1/JIP1 in organizing different components of the JNK pathway, including RAC1 or RAC2, MAP3K11/MLK3 or MAP3K7/TAK1, MAP2K7/MKK7, MAPK8/JNK1 and/or MAPK9/JNK2 into a functional multiprotein complex to ensure the effective activation of the JNK signaling pathway. Regulates the differentiation of CD4(+) and CD8(+) T-cells and promotes T-helper 1 (Th1) cell differentiation. Regulates the activation of MAPK8/JNK1 and MAPK9/JNK2 in CD4(+) T-cells and the activation of MAPK8/JNK1 in CD8(+) T-cells (PubMed:23963642, PubMed:27084103, PubMed:9482736). Plays a crucial role in the migration of neocortical neurons in the developing brain. Controls proper cortical neuronal migration and the formation of proximal cytoplasmic dilation in the leading process (PCDLP) in migratory neocortical neurons by regulating the proper localization of activated RAC1 and F-actin assembly (PubMed:22959435).[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220806 | Sh3rf1 (untagged) - Mouse SH3 domain containing ring finger 1 (cDNA clone MGC:63290 IMAGE:6405370), (10ug) |
CNY 9,120.00 |
|
| MG210987 | Sh3rf1 (tGFP-tagged) - Mouse SH3 domain containing ring finger 1 (cDNA clone MGC:63290 IMAGE:6405370) |
CNY 5,420.00 |
|
| MR210987L3 | Lenti ORF clone of Sh3rf1 (Myc-DDK-tagged) - Mouse SH3 domain containing ring finger 1 (cDNA clone MGC:63290 IMAGE:6405370) |
CNY 6,840.00 |
|
| MR210987L4 | Lenti ORF clone of Sh3rf1 (mGFP-tagged) - Mouse SH3 domain containing ring finger 1 (cDNA clone MGC:63290 IMAGE:6405370) |
CNY 6,840.00 |
