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Osbpl5 (BC079872) Mouse Tagged ORF Clone
CAT＃: MR211031
- TrueORF®
Osbpl5 (Myc-DDK-tagged) - Mouse oxysterol binding protein-like 5 (cDNA clone MGC:100260 IMAGE:6832073)
ORF Plasmid: tGFP
"BC079872" in other vectors (4)
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CNY 6,400.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ORP5, Osbp2, Obph1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211031 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGGAGGAGGCCTTTCTCCGGCGCCGTTTCTCGCTGTGTCCACCAGCTTCTACTCCACAGAAGACTG ACCCCCGGAAAGTCCCACGGAACCTGCTTCTGGGCTGCGAAAATGAGCTTGGTCCCATCACCCCAGGAAG GGACATGGAGTCCAACGGCCCATCACAACCCAGAGATGAAGAGCCCCAGACCCCAGGCTCCGCCACGAAG GTGCCACTGGCTGAGTACAGGCTATGCAATGGATCTGACAAGGAATGTACCTCTCCAACGACCAGGGTCT CCAAGAAGGATGCTCTCAAGGCACAGAAGGAGAACTATCGGCAGGAGAAGAAGCGGGCCACGAAGCAGCT GTTCAGTGCCCTGACTGACCCCAGTGTGGTCATCATGGCTGACAGCCTAAAGATCCGAGGGACCCTGAAG AGCTGGACGAAGCTGTGGTGTGTACTGAAGCCAGGGGTGCTGCTCATTTACAAGACACCCAAGGTGGGCC AGTGGGTGGGCACGGTGCTGCTGCACTGCTGCGAGCTTATTGAGCGCCCCTCCAAGAAGGACGGCTTCTG CTTCAAACTCTTCCACCCGCTGGACCAGTCTGTGTGGGCTGTGAAGGGCCCCAAAGGCGAGAGTGTGGGC TCCATCACTCAGCCCCTCCCTAGCAGCTACCTGATCTTCAGGGCCGCTTCTGAATCAGACGGCCGCTGCT GGCTAGATGCCCTGGAGCTGGCCCTTCGCTGCTCCAGCCTCCTGCGACTGAGCACGTGCAAGCAGGGTCG AGATGGGGAGCAGGGGTCCTCGCCTGATGCCTCTCCCTCTTCTCTCTACGGACTGCCCACCTCGGCCACC ATCCCAGATCAAGACCTGTTCCCGCTGAATGGGTCTGCCTTGGAGAACGATGCATTCTCAGACAAGTCAG AACGAGAGAATGCAGAAGACTCGGATGCCGAGACCCAGGATCACAGCCGCAAGACCAACGAGAGTGGGAG CGACCTGCTAGACAGCCCTGGTGGGCCATGGAGGGGAACTACCTATGTGGAACAGGTCCAGGAGGAGCTG GGAGAGCTGGACGAGACTTCCCAGGTGGAGACAGTGTCAGAGGAAAACAAGAGCCTGATGTGGGTGCTGC TGCGACAGCTGCGGCCAGGCATGGACCTGTCCCGTGTGGTGCTGCCGACCTTCGTGTTGGAGCCTCGGTC CTTCCTCGGCAAGCTCTCTGATTACTACTACCACGGAGACCTGCTCTCCAGGGCAGCAGCAGAGGATGAC CCCTACTGCCGCATGAAGCTTGTGCTCCGGTGGTACCTGTCTGGCTTCTATAAGAAGCCCAAGGGCATCA AGAAACCCTATAATCCCATTCTGGGGGAGACTTTTCGCTGCCGCTGGCTGCACCCTCAGACCAACAGTCA CACCTTCTACATAGCAGAACAGGTGTCTCACCATCCTCCTGTGTCTGCCTTCTATGTCAGCAACAGGAAA GATGGCTTCTGCATGAGTGGCAGCATCACAGCAAAGTCTAAGTTCTATGGGAACTCATTGTCGGCGCTTC TGGACGGCAAGGCTAAACTCACCTTCCTTAATCGCAAGGAGGAGTATACCCTCACCATGCCCTATGCCCA CTGCCGAGGGATCCTCTACGGCACCATGACCATGGAGCTGGGAGGCAAAGTGAACATCGAGTGTGAGAAG AACAACCTCCAAGCTGAACTGGACTTCAAGCTCAAGCCTTTCTTTGGAAGCAGCGCCAACATCAACCAGA TTTCCGGGAAGATCATGTCAGGAGAGGAAGTCCTGGCACGCCTCACTGGACACTGGGACAGAGATGTATT TATCAAGGAAGAGAGCAGTGGAGGCACCGAGCTCTTCTGGACTCCGAGTGAGGAGGTCCGCAGGCAGCGG CTGAAGCGGCACACAGTGCTGTTGGAAGAGCAGAGTGAACTGGAGTCTGAGAGGCTGTGGCAGCATGTCA CCAGGGCCATCCGTGAGGGTGACCAGCACAAAGCCACACAGGAGAAGTCTGTGCTGGAAGAGGCGCAGCG GCAGCGTGCCCGTGAGCACCAGCAGAGCCTCACTCCATGGAAGCCACAGTTGTTCTTACTGGACCCTCTC ACTCAGGAATGGCGCTACCGATATGAGGACCTCAGCCCCTGGGACCCGCTGAAGGACATTGCCCAATATG AGCAAGATGGGATTCTTCATACTCTACAGAGGGAGACTATGTCTGGCCAGACAACCTTTCTGGGGAGTCC TGACTCCAGGCACAAGAGGCCTAGCTCTGATCGGCGGCTCCGCAAAGCCAGTGACCAGCCCTCTGGCCAT AGCCAGGTCACTGAGAGCAGCGGCTCCACACCTGAGTCCTGCCCAGACCTCTCTGATGAGGACTTTGTTC CTGGTGGTGAGAGCCCATGTCCTCGGTGCAGGCGAGAGGTACACCGGCTAAAGATGCTACAAGAGGCCGT GCTGTCCATTCAGGAAGCTCAGCAAGAGCTGCATAGGCACCTTTCGACCATGTTAAGCTCCACAGTGCGT GCTGGGCAGGCGCCAGCCCCCAGCCTCCTGCAGAATCCCCGCTCCTGGTTCCTGCTCTGCATCTTCCTGA CGTGTCAGCTATTCATTAACTATATCCTCAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211031 protein sequence
Red=Cloning site Green=Tags(s) MKEEAFLRRRFSLCPPASTPQKTDPRKVPRNLLLGCENELGPITPGRDMESNGPSQPRDEEPQTPGSATK VPLAEYRLCNGSDKECTSPTTRVSKKDALKAQKENYRQEKKRATKQLFSALTDPSVVIMADSLKIRGTLK SWTKLWCVLKPGVLLIYKTPKVGQWVGTVLLHCCELIERPSKKDGFCFKLFHPLDQSVWAVKGPKGESVG SITQPLPSSYLIFRAASESDGRCWLDALELALRCSSLLRLSTCKQGRDGEQGSSPDASPSSLYGLPTSAT IPDQDLFPLNGSALENDAFSDKSERENAEDSDAETQDHSRKTNESGSDLLDSPGGPWRGTTYVEQVQEEL GELDETSQVETVSEENKSLMWVLLRQLRPGMDLSRVVLPTFVLEPRSFLGKLSDYYYHGDLLSRAAAEDD PYCRMKLVLRWYLSGFYKKPKGIKKPYNPILGETFRCRWLHPQTNSHTFYIAEQVSHHPPVSAFYVSNRK DGFCMSGSITAKSKFYGNSLSALLDGKAKLTFLNRKEEYTLTMPYAHCRGILYGTMTMELGGKVNIECEK NNLQAELDFKLKPFFGSSANINQISGKIMSGEEVLARLTGHWDRDVFIKEESSGGTELFWTPSEEVRRQR LKRHTVLLEEQSELESERLWQHVTRAIREGDQHKATQEKSVLEEAQRQRAREHQQSLTPWKPQLFLLDPL TQEWRYRYEDLSPWDPLKDIAQYEQDGILHTLQRETMSGQTTFLGSPDSRHKRPSSDRRLRKASDQPSGH SQVTESSGSTPESCPDLSDEDFVPGGESPCPRCRREVHRLKMLQEAVLSIQEAQQELHRHLSTMLSSTVR AGQAPAPSLLQNPRSWFLLCIFLTCQLFINYILK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC079872 |
| ORF Size | 2622 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC079872, AAH79872 |
| RefSeq Size | 3834 bp |
| RefSeq ORF | 2624 bp |
| Locus ID | 79196 |
| MW | 98.9 kDa |
| Gene Summary | Lipid transporter involved in lipid countertransport between the endoplasmic reticulum and the plasma membrane: specifically exchanges phosphatidylserine with phosphatidylinositol 4-phosphate (PI4P), delivering phosphatidylserine to the plasma membrane in exchange for PI4P, which is degraded by the SAC1/SACM1L phosphatase in the endoplasmic reticulum. Binds phosphatidylserine and PI4P in a mutually exclusive manner. May cooperate with NPC1 to mediate the exit of cholesterol from endosomes/lysosomes. Binds 25-hydroxycholesterol and cholesterol.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220664 | Osbpl5 (untagged) - Mouse oxysterol binding protein-like 5 (cDNA clone MGC:100260 IMAGE:6832073), (10ug) |
CNY 9,120.00 |
|
| MG211031 | Osbpl5 (tGFP-tagged) - Mouse oxysterol binding protein-like 5 (cDNA clone MGC:100260 IMAGE:6832073) |
CNY 5,420.00 |
|
| MR211031L3 | Lenti ORF clone of Osbpl5 (Myc-DDK-tagged) - Mouse oxysterol binding protein-like 5 (cDNA clone MGC:100260 IMAGE:6832073) |
CNY 6,840.00 |
|
| MR211031L4 | Lenti ORF clone of Osbpl5 (mGFP-tagged) - Mouse oxysterol binding protein-like 5 (cDNA clone MGC:100260 IMAGE:6832073) |
CNY 6,840.00 |
