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Exosc10 (NM_016699) Mouse Tagged ORF Clone
CAT＃: MR211065
- TrueORF®
Exosc10 (Myc-DDK-tagged) - Mouse exosome component 10 (Exosc10)
ORF Plasmid: tGFP
"NM_016699" in other vectors (4)
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CNY 9,744.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | p2; p3; p4; PM-Scl; PM/Scl-100; Pmscl2; RRP6 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211065 representing NM_016699
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCCTCCCAGTCCCCGGGAGCATCAGTCCGCGCCGGCGACCAGTGCGACCAAGCCTGACGCGGAGA TGGTACTGCCTGGCTTCCCCGATGCAGACAGCTTCGTAAAGTTTGCACTTGGGTCGGTAGTGGCAGTTAC CAAGGCATCCGGGGGCCTGCCACAGTTCGGTGACGAGTATGATTTTTACAGAAGTTTCCCTGCCTTCCAG GCATTCTGTGAGACACAAGGAGACAGGTTACTGCAGTGCATGAGTCGGGTAATGCAGTACCATGGCTGTC GCAGCAACATCAAAGACCGAAGTAAAGTGACTGAATTGGAGGACAAGTTTGATTTATTAGTCGATACCAA TGACGTGATATTGGAGAGAGTGGGCATGTTACTGGATGAAGCCTCCGGTGTGAACAAGCATCAGCAGCCT GTCCTTCCTGCAGGGCTGCAGGTCCCCAAAACAATAGTATCCAGCTGGAATCGGAAGGCAGGAGAGTATG GCAAAAAGGCAAAATCGGAGACTTTCCGACTGCTGCATGCAAAAAACATCGTGCGACCTCAGCTCAGGTT TCGAGAGAAGATCGACAATTCTAACACACCATTTCTCCCGAAGATCTTTGTCAAACCCAATGCCCGGAAG CCACTCCCTCTGGCTCTCTCAAAAGAAAGGCGGGAACGCCCGCAGGACCGTCCGGAGGACCTGGATGTCC CCCCAGCCCTGGCAGATTTCATCCATCAGCAGCGAACCCAGCAGGTGGAGCAGGACATGTTTGCACACCC TTACCAGTATGAACTGGATCACTTTACTCCGCCTCAGTCGGTGCTGCAGAGGCCGAAGCCCCAGTTGTAC CGAGCTGTGGGAGAGACTCCCTGCCACTTGGTGTCGTCCCTGGATGAGCTGGTGGAGCTCAACGAGAAGC TCCTGGGCTGTCAGGAGTTTGCCGTGGACTTAGAGCATCACTCTTACAGAAGCTTCCTAGGACTCACCTG CCTCATGCAGATCTCCACCCGGACAGAAGACTTCATTGTCGACACCCTCGAGCTTCGCAGTGACATGTAC ATTCTCAACGAGAGCCTCACGGACCCAGCCATCGTTAAGGTCTTCCATGGTGCCGACTCTGACATTGAGT GGCTGCAGAAAGACTTCGGACTCTATGTGGTGAACATGTTTGACACACACCAGGCAGCACGGCTTCTCAA CCTGGCTCGGCACTCACTCGACCATCTGCTGAGACTCTACTGCGGTGTGGAATCAAACAAGCAATATCAG CTGGCAGACTGGAGGATACGTCCTCTGCCAGAGGAAATGCTGAGCTACGCCCGGGATGACACCCATTACC TGCTCTACATCTATGACCGAATGAGGCTGGAACTGTGGGAAAGAGGCAACCACCAGCCTGTCCAGTTGCA GGTGGTTTGGCAGCGGAGCAGGGACATCTGCCTCAAGAAATTTGTCAAGCCTATCTTTACGGACGAGTCC TACCTGGAGCTCTATCGAAAGCAAAAGAAGCACCTGAACTCGCAGCAGCTGACCGCCTTCCAGCTGCTGT TTGCTTGGAGGGATAAGACAGCTCGCAGGGAGGATGAGAGCTACGGATATGTTCTACCAAATCACATGAT GCTAAAGATAGCTGAGGAACTGCCTAAGGAGCCTCAAGGCATCATAGCTTGCTGTAACCCAGTACCACCT CTTGTCCGGCAGCAGATCAATGAGATGCATCTCCTAATCCAGCAGGCTCGAGAGATGCCCCTGCTCAAGT CTGAAAATGCAGCTGGAGTGAGGAAGAGCGGACCACTGCCTAGCGCCGAGAGATTGGAGAATGATCTCTT TGGACCCCATGACTGTTCCCACGCCCCTCCAGATAACTACCAGAACACCTCAACTGATGGGACCCTGCCA CTTCAGAAGCAGCCAAGCCTATTCACTGAGGGCAAAGAAGAGACCTCTGTGGATGCCGGATGCCTCCTTG CCACAGCCGTCATCACTCTGTTCAGTGAACCTAACACTGAGGAAGGTGGAAAGACTCCATTGACGGTGGC CCAGAAAAAAGCCCAGAACATAATGCAGTCCTTTGAAAACCCATTTCGGATGTTCCTGCCTTCTCTGGAA CACAAGGCCCACATCTCTCAAGCAGCAAAGTTTGATCCTTCTTCGAAAATCTATGAAATCAGCAACCGCT GGAAGCTGGCCAGCCAGGTCCAGGTCCAGAAAGAACCTAAAGAAGCAACCAAGAAGAAGGTAGCTGAGCA AACAGCTGCCCGGGAGGAGGCGAAGGAGGAGGCTGCAGCCGGTGTGCTGGAACAGGCTATCCCTGTGCGG CAGCAGGCCGCGCTAGAAAATGCTACTAAGAAGAGAGAACGAGCCACCAGTGACCTGAGGACTATAGAGC AAAAACAGGAGAAGAAACGACTAAAAAGCTCCAAGAAAGCCAAGGACCCTGATCCCCCAGGGAAAGACTT CAGTCCCTATGACTACAGCCAGTCAGACTTCAGGGCCTTTGCTGGGGACAGTAAATCCAAGCCTTCCTCC CAGTTCGACCCCAATAAGCTGGCACCTTCCGGCAAGAAAGGTGTTGGAGCCAAAAAATGTAAACAGTCGG TGGGAAACAAAAGCATGTCATTTGCTGTTGGAAAGTCGGACAGAGGCTTCCGCCACAACTGGCCAAAGAG A ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211065 representing NM_016699
Red=Cloning site Green=Tags(s) MAPPSPREHQSAPATSATKPDAEMVLPGFPDADSFVKFALGSVVAVTKASGGLPQFGDEYDFYRSFPAFQ AFCETQGDRLLQCMSRVMQYHGCRSNIKDRSKVTELEDKFDLLVDTNDVILERVGMLLDEASGVNKHQQP VLPAGLQVPKTIVSSWNRKAGEYGKKAKSETFRLLHAKNIVRPQLRFREKIDNSNTPFLPKIFVKPNARK PLPLALSKERRERPQDRPEDLDVPPALADFIHQQRTQQVEQDMFAHPYQYELDHFTPPQSVLQRPKPQLY RAVGETPCHLVSSLDELVELNEKLLGCQEFAVDLEHHSYRSFLGLTCLMQISTRTEDFIVDTLELRSDMY ILNESLTDPAIVKVFHGADSDIEWLQKDFGLYVVNMFDTHQAARLLNLARHSLDHLLRLYCGVESNKQYQ LADWRIRPLPEEMLSYARDDTHYLLYIYDRMRLELWERGNHQPVQLQVVWQRSRDICLKKFVKPIFTDES YLELYRKQKKHLNSQQLTAFQLLFAWRDKTARREDESYGYVLPNHMMLKIAEELPKEPQGIIACCNPVPP LVRQQINEMHLLIQQAREMPLLKSENAAGVRKSGPLPSAERLENDLFGPHDCSHAPPDNYQNTSTDGTLP LQKQPSLFTEGKEETSVDAGCLLATAVITLFSEPNTEEGGKTPLTVAQKKAQNIMQSFENPFRMFLPSLE HKAHISQAAKFDPSSKIYEISNRWKLASQVQVQKEPKEATKKKVAEQTAAREEAKEEAAAGVLEQAIPVR QQAALENATKKRERATSDLRTIEQKQEKKRLKSSKKAKDPDPPGKDFSPYDYSQSDFRAFAGDSKSKPSS QFDPNKLAPSGKKGVGAKKCKQSVGNKSMSFAVGKSDRGFRHNWPKR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_016699 |
| ORF Size | 2661 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_016699.3 |
| RefSeq Size | 2803 bp |
| RefSeq ORF | 2664 bp |
| Locus ID | 50912 |
| UniProt ID | P56960 |
| MW | 101.4 kDa |
| Gene Summary | Putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. EXOSC10 has 3'-5' exonuclease activity (By similarity). EXOSC10 is required for nucleolar localization of C1D and probably mediates the association of MTREX, C1D and MPP6 wth the RNA exosome involved in the maturation of 5.8S rRNA (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222420 | Exosc10 (untagged) - Mouse exosome component 10 (Exosc10), (10ug) |
CNY 11,500.00 |
|
| MG211065 | Exosc10 (tGFP-tagged) - Mouse exosome component 10 (Exosc10) |
CNY 11,344.00 |
|
| MR211065L3 | Lenti ORF clone of Exosc10 (Myc-DDK-tagged) - Mouse exosome component 10 (Exosc10) |
CNY 10,740.00 |
|
| MR211065L4 | Lenti ORF clone of Exosc10 (mGFP-tagged) - Mouse exosome component 10 (Exosc10) |
CNY 10,740.00 |
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