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Limch1 (BC075634) Mouse Tagged ORF Clone
CAT＃: MR211106
- TrueORF®
Limch1 (Myc-DDK-tagged) - Mouse LIM and calponin homology domains 1 (cDNA clone MGC:100029 IMAGE:30544497)
ORF Plasmid: tGFP
"BC075634" in other vectors (4)
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CNY 6,600.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | mKIAA1102 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211106 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCACAAAACATGTACAATACGGTCCTTCATACGCGACTTTTATAGAATCCTTTTTACATGCGCAAGGC CATCCATTATGTCAGATGACGCAGAGAGCACGAGTATGTTTGACATGCGGTGTGAGGAGGAGGCAGCGGT GCTGCCGCACAGCAGGGCCCGCCAGGAGCAGCTGCAGCCCGAACCACCTCCGGAGCCTGCCTTCGCCGAG GCACAGAAGTGGATCGAGCAAGTCACTGGTCGAAGTTTTGGTGACAAAGACTTCCGGACAGGACTAGAAA ATGGGATCCTCCTTTGCGAGTTGCTGAATGCTATAAAGCCAGGACTTGTTAAAAAGATCAATAGATTGCC TACCCCCATTGCAGGATTGGACAATACCATCTTATTCTTGAGAGGCTGTAAGGAGCTTGGCCTTAAAGAA TCTCAACTCTTTGACCCGAGTGACCTCCAGGATACCTCCAACAGGGTCACTGTCAAGAACCTCGATTATA GCAGGAAGCTGAAAAACGTGCTAGTGACCATTTACTGGCTGGGGAAAGCTGCAAACAGCTGTGCATCCTA CGGTGGAACCACACTGAACCTGAAGGAGTTTGAAGGGCTGTTGGCTCAGATGCGAAAGGAGACTGACGAC ATTGATAGCCCGAAGCGCAGCATCCGAGACAGTGGCTACATCGACTGCTGGGACTCTGAACGCAGCGACT CCCTCTCTCCTCCTCGCCACGGACGGGAAGATGGGACAAGTGAGCGGAGGAAAAGCATCAAAACTTACAG AGAAATCGTCCAAGAAAAAGAGCGGAGAGAGAGGGAACTTCATGAGGCGTACAAGAACGCAAGGTCGCAG GAGGAGGCCGAGGGGATCCTCCAGCAGTACATCGAGAGGTTCACCATCAGTGAGGCTGTCCTCGAACGCT TGGAGATGCCAAAAATCCTGGAAAGAAGCCATTCAACAGAGCCAAACGTGTCCTCCTTCCCAAACGACCC CAGCCCCATGAAGTACCTGCGGCAGCAGTCACTGCCCCCACCCAAGTTCACTGCCACGGTTGAGACCACC ATAGCTCGCACCAGTGTCCCAGAGAGCATTGCGTCAGCAGGCACTGGGTCTCCAAGCAAAATCATCACTC CAAACACAGTGCCTATGCTGACACCCAGGCCTTACTCCCAGCCCAAAAACTCTCAAGAGGTTCTGAAGAC TTTTAAGGTAGATGGGAAGGTCAGCATGAATGGGGAAATGGCCCCTGGAGATGAGGAAGGAAAGGAAAAA GAAGGCCCTGCAGCTGCAGCCCCTGGCCCTTCCTTAACCAAGTCCCAGATGTTTGAAGGTGTGGCCACGG TGCACGACTCTCCTGTGCAGGTGAAACAAGGCAGCAACAGCATCGAGATCAACATCAAGAAGCCAAATTC TGCTCCCCAGGAACTGACAGCAGCCTCTGAGGAAACTGAATCGAACGGCCAAGAGGATGAAGACGGTGAA GAAAGGCCAGGGACAGGGGATTTGGAGCCTGACTCGGCAGAACCCCAGCACTTTACGACAACTGTGACTC GGTGCAGCCCAACAGTGGCCTTGGTGGAGTTTTCACCCAACCCGCAGCTGAAGAATGAGGTGCCAGAACA AGGCCAGAAGAAGCCAGAAGATGAGATGAGTGGGAAGGTGGAGTTAGTGTTGTCACAGAAGGTGGCAAAG CCAAAATCCCCAGAGCCTGAAGCAACCCTGACGTTTCCATTTCTCGACAAAATGCCTGAAACCAACCAAC TACATTTGCCAAATCCCAGTTCTCAAGCGGATTCTCCAAGCAGTGAAAAGTCCCCTGGGAGCACACCCTT TAAGTTCTGGGCATGGGACCCAGAAGAGGAGCGCCGGCGACAGGAGAAATGGCAGCAGGAGCAGGAACGT CTGCTGCAGGAGAGGTATCAGAAGGAGCAAGACAAGCTGAAGGAGGAGTGGGAAAAGGCCCAGAAGGAGG TCGAAGAAGAAGAACGCAGATACTACGAGGAGGAGCGTAAGATAATTGAAGACACGGTGGTTCCATTCAC TATTTCCTCAAGCTCTGCAGACCAACTGTCTACGTCCTTGTCTGTAACTGAAGGCAGCGGGACAAGGAAT AAGATGGACTTGGAAAACTGCCCAGACAAAGAAAACGAGAGAAGACAGAAGACGCCTTTCCAGGAGAATG ACGGTGATTCATTGCTCAAAACTAGAGAAGGTGGTCTGCCAGAGGAGCAGAGCCTAACTCCAAGTCCCTC GGCTAACCCTGAAATCTCCGTGTCAAAAGGAATCCATCAGGACCCGCAGCTGGAGGCAGAGGCTGGGGCC CCACACTGTGGTACAAACCCACAGCCAGCTCAGGATCCACCTCGGAATCAACAGATACCAAACCCACCAA CATCCACGTCGGAAGATGTGAAGCCCAAAACCTTGGCCCTGGAGAAAACCATCAATCATCAGATGGAGTC TCCGGGGGAAAGGCGGAAGTCTATAAGCGGGAAGAAACTGTGCTCCTCCTGCGGGCTCACTTTGGGTAAA GGTGCTGCGATGATCATCGAGACCCTGAATCTCTACTTTCACATTCAGTGTTTCAGGTGCGGCATCTGTA AAGGACAGCTCGGAGATGCAGTAAGCGGGACAGACGTCAGGATTCGCAATGGTCTCCTAAACTGTACCGA CTGCTACATGCGATCCAGAAGTGCCGGCCAGCCTACAACACTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211106 protein sequence
Red=Cloning site Green=Tags(s) MHKTCTIRSFIRDFYRILFTCARPSIMSDDAESTSMFDMRCEEEAAVLPHSRARQEQLQPEPPPEPAFAE AQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNTILFLRGCKELGLKE SQLFDPSDLQDTSNRVTVKNLDYSRKLKNVLVTIYWLGKAANSCASYGGTTLNLKEFEGLLAQMRKETDD IDSPKRSIRDSGYIDCWDSERSDSLSPPRHGREDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQ EEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNVSSFPNDPSPMKYLRQQSLPPPKFTATVETT IARTSVPESIASAGTGSPSKIITPNTVPMLTPRPYSQPKNSQEVLKTFKVDGKVSMNGEMAPGDEEGKEK EGPAAAAPGPSLTKSQMFEGVATVHDSPVQVKQGSNSIEINIKKPNSAPQELTAASEETESNGQEDEDGE ERPGTGDLEPDSAEPQHFTTTVTRCSPTVALVEFSPNPQLKNEVPEQGQKKPEDEMSGKVELVLSQKVAK PKSPEPEATLTFPFLDKMPETNQLHLPNPSSQADSPSSEKSPGSTPFKFWAWDPEEERRRQEKWQQEQER LLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTISSSSADQLSTSLSVTEGSGTRN KMDLENCPDKENERRQKTPFQENDGDSLLKTREGGLPEEQSLTPSPSANPEISVSKGIHQDPQLEAEAGA PHCGTNPQPAQDPPRNQQIPNPPTSTSEDVKPKTLALEKTINHQMESPGERRKSISGKKLCSSCGLTLGK GAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCTDCYMRSRSAGQPTTL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC075634 |
| ORF Size | 2703 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC075634 |
| RefSeq Size | 4698 bp |
| RefSeq ORF | 2705 bp |
| Locus ID | 77569 |
| MW | 100.7 kDa |
| Gene Summary | Actin stress fibers-associated protein that activates non-muscle myosin IIa. Activates the non-muscle myosin IIa complex by promoting the phosphorylation of its regulatory subunit MRLC/MYL9. Through the activation of non-muscle myosin IIa, positively regulates actin stress fibers assembly and stabilizes focal adhesions. It therefore negatively regulates cell spreading and cell migration.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220852 | Limch1 (untagged) - Mouse LIM and calponin homology domains 1 (cDNA clone MGC:100029 IMAGE:30544497), (10ug) |
CNY 9,120.00 |
|
| MG211106 | Limch1 (tGFP-tagged) - Mouse LIM and calponin homology domains 1 (cDNA clone MGC:100029 IMAGE:30544497) |
CNY 5,610.00 |
|
| MR211106L3 | Lenti ORF clone of Limch1 (Myc-DDK-tagged) - Mouse LIM and calponin homology domains 1 (cDNA clone MGC:100029 IMAGE:30544497) |
CNY 7,030.00 |
|
| MR211106L4 | Lenti ORF clone of Limch1 (mGFP-tagged) - Mouse LIM and calponin homology domains 1 (cDNA clone MGC:100029 IMAGE:30544497) |
CNY 7,030.00 |
