Fig4 (NM_133999) Mouse Tagged ORF Clone
CAT#: MR211140
- TrueORF®
Fig4 (Myc-DDK-tagged) - Mouse FIG4 homolog (S. cerevisiae) (Fig4)
ORF Plasmid: tGFP
"NM_133999" in other vectors (4)
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CNY 9,976.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | A530089I17Rik; AI326867; Sac3 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR211140 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCACGGCCGCTGCCCCCATCATCAGCTCGGTCCAGAAGCTGGTGCTGTATGAGACCAGAGCTAGAT ACTTTCTAGTTGGGAGCAATCATGCAGAAACGAAGTACCGTGTCCTGAAAATTGATAGAACAGAACCGAA GGACTTGGTGGTAATCGACGACAGGCACGTGTACACACAACAAGAAGTGAGGGAACTTCTTGGCCGCCTG GATCTGGGAAACAGAACAAAGATGAGCCAGAAAGGATCCTCCGGGTTGTTTCGAGCCGTCTCAGCTTTTG GAGTCGTAGGTTTTGTCAGGTTCTTAGAAGGCTACTATATTGTATTAATAACTAAGCGGAGGAAGATGGC AGACATTGGAGGTCATGCAATTTATAAGATTGAAGATACAAGTATGATTTATATCCCCAATGACTCTGTT CGGATTTCTCACCCCGACGAAGCTAGGTATCTAAGGATATTTCAAAATGTGGATCTATCTAGCAATTTTT ACTTTAGTTACAGCTATGATTTGTCACACTCACTTCAGTATAATCTTACCGTCCTGCGAATGCCCCTGGA GATGTTAAAGTCAGAAACATCCAAGGCGTGCCAGGAGAGCTTCGACATCTTTGAAGATGAAGGATTAATT ACACAGGGTGGCAGTGGTGTATTTGGGATCTCTAGTGAGCCTTATATGAAGTATGTATGGAACGGTGAAC TTCTGGATATAATTAAAAACACTGTGCATCGTGACTGGCTATTGTATATCATTCATGGGTTCTGCGGGCA GTCCAAGCTTTTGATCTATGGACGACCAGTGTATGTGACCCTAATAGCCAGGAGATCCAGTAGGTTTGCT GGGACGCGCTTTCTCAAGAGAGGCGCAAACTGTGAGGGTGACGTTGCAAATGAGGTGGAGACCGAGCAGA TCCTCTGTGATGCTTCTGTGATGTCTTTTACCGCAGGCAGTTACTCTTCTTACGTACAAGTTAGAGGATC CGTTCCCTTATTCTGGTCCCAAGACATCTCAACTATGATGCCGAAACCACCCATTACACTGGACCAGGCG GATCCCTTTGCACACGTGGCTGCTCTTCACTTTGACCAGATGCTCCAGAGGTTCGGCTCCCCCATCATCA TCTTGAACTTAGTGAAGGAGAGGGAGAAGAGAAAGCACGAAAGGATCCTGAGTGAAGAGCTGGTGGCTGC GGTCACTTACCTGAACCAGTTCCTGCCACCGGAGCACACCATTGTCTACATCCCCTGGGACATGGCCAAG TACACCAAGAGTAAGCTGTGCAACGTTCTCGATCGGCTGAATGTGATTGCGGAAAGCGTGGTAAAGAAGA CAGGATTCTTTGTAAACCGCCCTGATTCTTACTGCAGCATTTTACGGCCAGATGAAAAGTGGAATGAACT AGGAGGTCATGTGATTCCCACTGGTCGGCTCCAGACTGGCATCCTTCGAACCAACTGTGTGGACTGTTTG GATCGCACCAACACTGCGCAGTTCATGGTGGGCAAGTGTGCCCTGGCTTACCAGCTGTATTCCTTAGGGC TGATTGACAAGCCTAACCTGCAGTTCGACACGGATGCAGTGAGGTTGTTTGAGGAACTCTATGAAGACCA TGGAGACACCCTTTCCCTGCAGTACGGAGGCTCTCAGCTGGTTCATCGGGTAAAGACCTACAGAAAGATA GCACCGTGGACCCAGCACTCCAAGGACATCATGCAGACTCTGTCCCGGTACTACAGCAACGCCTTCTCGG ATGCTGATAGACAAGATTCCATCAACCTGTTTTTGGGTGTTTTCCACCCCACAGAAGGGAAACCTCATCT CTGGGAACTCCCCACAGACTTTTACCTCCATCACAAAAACACCATGAGCCTTTTACCCCCCAGAAGGAGT TATACTTACTGGTGGACGCCAGAGGTGGTGAAGCATCTGCCGCTGCCTTACGATGAAGTTATCTGTGCTG CAAACTTAAAGAAGTTGATGGTGAAGAAATTTCACAGATGGGAAGAAGAGATTGATATTCACAATGAGTT CTTCCGGCCGTATGAATTGAGTAGTTTTGATGACACCTTTTGCTTGGCCATGACAAGTTCAGCACGTGAT TTTATGCCTAAAACTGTTGGCATTGATCCAAGTCCATTTACCGTGCGAAAACCAGATGAAACTGGAAAAT CTGTACTGGGGAACAAAAACACTAGAGAGGAAGCTGTCCTGCAGCGGAAGACGGCAGCCAGCGCCCCGCC ACCCCCGAGCGAGGAGGCTGTGTCCAGCAGCTCTGAGGATGACTCTGGCACCGACCGGGAAGATGAAGGC TCCATCTCTCAGCGATCCACTCCTGTGAAGATGACTGACACGGGAGACAGTGCCAAAGCGACTGAGAATG TGGTCCAGCCCATGAAGGAAGTGTATGGAGTCAGTCTCTCCAGTAGCCTGTCAGAGGAGGATCACTCCAT TTATGCCAGGTTTGTTCAGCTGGGGCAAAGTCAACATAAACAGGACCGAGGTAACCAGCAGCTGTGTTCC CGTTGCTCAGATGGAGTTATAAAACTAACTCCCATCTCAGCTTTCTCGCAAGACAACATTTATGAAGTTC AGCCTCCAAGAGTAGACAGAAAGTCTACAGAGATCTTCCAGGCCCATATCCAAGCTAGCCAAGGTATCAT GCAGCCCCTTGGCAAAGAGGACACTGCCATGTACCGGGAGTACATCAGAAACCGCTACTTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211140 protein sequence
Red=Cloning site Green=Tags(s) MPTAAAPIISSVQKLVLYETRARYFLVGSNHAETKYRVLKIDRTEPKDLVVIDDRHVYTQQEVRELLGRL DLGNRTKMSQKGSSGLFRAVSAFGVVGFVRFLEGYYIVLITKRRKMADIGGHAIYKIEDTSMIYIPNDSV RISHPDEARYLRIFQNVDLSSNFYFSYSYDLSHSLQYNLTVLRMPLEMLKSETSKACQESFDIFEDEGLI TQGGSGVFGISSEPYMKYVWNGELLDIIKNTVHRDWLLYIIHGFCGQSKLLIYGRPVYVTLIARRSSRFA GTRFLKRGANCEGDVANEVETEQILCDASVMSFTAGSYSSYVQVRGSVPLFWSQDISTMMPKPPITLDQA DPFAHVAALHFDQMLQRFGSPIIILNLVKEREKRKHERILSEELVAAVTYLNQFLPPEHTIVYIPWDMAK YTKSKLCNVLDRLNVIAESVVKKTGFFVNRPDSYCSILRPDEKWNELGGHVIPTGRLQTGILRTNCVDCL DRTNTAQFMVGKCALAYQLYSLGLIDKPNLQFDTDAVRLFEELYEDHGDTLSLQYGGSQLVHRVKTYRKI APWTQHSKDIMQTLSRYYSNAFSDADRQDSINLFLGVFHPTEGKPHLWELPTDFYLHHKNTMSLLPPRRS YTYWWTPEVVKHLPLPYDEVICAANLKKLMVKKFHRWEEEIDIHNEFFRPYELSSFDDTFCLAMTSSARD FMPKTVGIDPSPFTVRKPDETGKSVLGNKNTREEAVLQRKTAASAPPPPSEEAVSSSSEDDSGTDREDEG SISQRSTPVKMTDTGDSAKATENVVQPMKEVYGVSLSSSLSEEDHSIYARFVQLGQSQHKQDRGNQQLCS RCSDGVIKLTPISAFSQDNIYEVQPPRVDRKSTEIFQAHIQASQGIMQPLGKEDTAMYREYIRNRYL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_133999 |
ORF Size | 2724 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_133999.1, NP_598760.1 |
RefSeq Size | 3278 bp |
RefSeq ORF | 2724 bp |
Locus ID | 103199 |
UniProt ID | Q91WF7 |
MW | 103.4 kDa |
Gene Summary | The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). In vitro, hydrolyzes all three D5-phosphorylated polyphosphoinositide substrates in the order PtdIns(4,5)P2 > PtdIns(3,5)P2 > PtdIns(3,4,5)P3. Plays a role in the biogenesis of endosome carrier vesicles (ECV) / multivesicular bodies (MVB) transport intermediates from early endosomes.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC205750 | Fig4 (untagged) - Mouse FIG4 homolog (S. cerevisiae) (Fig4), (10ug) |
CNY 6,648.00 |
|
MG211140 | Fig4 (tGFP-tagged) - Mouse RIKEN cDNA A530089I17 gene (A530089I17Rik) |
CNY 11,576.00 |
|
MR211140L3 | Lenti ORF clone of Fig4 (Myc-DDK-tagged) - Mouse FIG4 homolog (S. cerevisiae) (Fig4) |
CNY 9,030.00 |
|
MR211140L4 | Lenti ORF clone of Fig4 (mGFP-tagged) - Mouse FIG4 homolog (S. cerevisiae) (Fig4) |
CNY 9,030.00 |