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Chaf1a (NM_013733) Mouse Tagged ORF Clone
CAT＃: MR211155
- TrueORF®
Chaf1a (Myc-DDK-tagged) - Mouse chromatin assembly factor 1, subunit A (p150) (Chaf1a)
ORF Plasmid: tGFP
"NM_013733" in other vectors (4)
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CNY 6,672.00
CNY 7,130.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AL023013; AL024058; Cac1p; CAF-1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211155 representing NM_013733
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGGAGGAGCCGGAGGCCGCGACGCGGACAGCCGCGGCCGTGGATTGCAAAGACAGACCCGGGTTCC CAGTGAAGAGGTTAATACAAGCCCGCTTGCCCTTCAAACGCCTGAACCTTGTTCCCAAGGAGAAAGTTGA GGAAGACACATCACCAAAAGCAGCTGTGGAAAGCAAAGTCCCGGATCTACAATTGTCTTTGGGCACTTTT GAAAGCCAGTGTCACACAGGCTCTCACGTAGGCTTGAGTACAAAGTTGGTCGGTGGCCAGGGCCCCATTG ATAGCTTCTTGAGGGCAACAATCAAGCCAGTACCGAGTGTGGTCATTATCGACCTGACGGAGAACTGCAG TGACATTCCAGACAGCCCTGAGGGCCACAGTGAACTAAGTCCTGACACTGCTGGTGTGGTCACCACAGTG GAAGGAGCTGCCAAGCAGCAGGAGCACTCTGCAGCAGAGCTGTGTCTCCTAGAGACCCCTTCAGACATCA CTTGTCACATGGAAGAGGAGCCTGGGAGTCCAGGAGACCCAAAGAGGACGGGTGACTGTCAGGCTGGTTC ACTACAGAGCTGTCCGGAGCTGACCCCAGGTTCTAGGACATGCCCCACCAAGGAGTTATCAAGCTGGAGC AAGGCAGGGGATCTCCTATTTATAGAGAAAGTGCCTGTGGTGGTGCTAGAGGACATCCTGGCCACTAAGC CCTCCATTGCCTCTCTTCCCATGATGTCCCTGGATAGGAGTGTCACTTCAGAGAGTGAAATACTGGAGTC CTGCCCCGAGGATGACTCCATACTGAGCCACTCCTCCACCAACTCCTCTTCTCCAACCAGCTCTCCTGAG GGACCATCCACACCCCCAGAACATCGGGGTGGGAGAAGCTCCCCCTCCACCCCAGCCTGCAGAGTTGCCA AGAACTTTGTCAAAGGCTCCACAGAGAAGGGCAGGAGCAAACTGCACAGAGATAGAGAACAGCAGAGGGA AGAGAAGGAAAAGCTGAGGGAAGAGATACGACGAGCCAAGGAGGAGGCACGGAAGAAGAAGGAGGAGGAG AAGGAGCTGAAGGAAAAGGAGCGGAGGGAAAAGCGGGAGAAAGATGAGAAGGAGAAGGCGGAGAAGCAGC GGCTAAAGGAGGAAAAACGCAAGGAGCGGCAGGAAGCTCTGGAGGCTAAACTGGAGGAGAAGAGGAAGAA GGAGGAAGAGAAGCGGTTACGGGAGGAAGAGAAGCGGTTACGGGAAGAAGAGAAGCGCATTAAGGCAGAA AAGGCAGAGATCACTCGATTCTTCCAGAAACCGAAGACTCCGCAGGCTCCCAAGACCCTGGCCGGTTCCT GTGGTAAGTTTGCCCCCTTTGAAATCAAAGAACACATGGTGCTGGCTCCACGGTGCCGAGCTGCCTTGGA TCAAGACCTGTGCGATCAGCTGGACCAGCTCCTGCAGCAGCAGAGTGTGGCAAGCACCTTCCTCAGTGAC CTAAAAAGCCGGCTGCCCTTGAGATCGGGGCCCACCCGGGTCTGTGGCCATGACACAGACATCATGAACA GGGATGTGGTCATTGTGGAGAGCAGCAAGGTGGACGGTGTGTCTGAAAGGAAGAAGTTTGGCCGCATGAA GCTTCTGCAGTTCTCAGAGAACCACCGGCCAGCATACTGGGGGACGTGGAACAAGAAGACAGCCATCATC CGCCCCCGGAACCCCTGGGCCCAGGACAAAGACCTTCTTGACTATGAGGTAGACAGTGACGATGAGTGGG AAGAGGAGGAACCTGGGGAGTCTTTGTCCCACAGTGAGGGGGATGAGGATGACGATGTGGGGGAAGATGA GGATGAGGACGATGGCTTCTTTGTGCCCCATGGGTACTTATCTGAAGATGAAGGCGTGACTGAGGAGTGT GCAGACCCTGAGAACCACAAAGTACACCAGAAGCTGAAGGCCAAGGAGTGGGATGAGCTCCTAGCCAAGG GCAAGCGCTTTCGTGTGCTGCAGCCTGTGCACGTGGGATGTGTATGGGCATCAGAGGCAGCCAACTGCAC GAGTTCCGACTTGAAGCTGCTGCAGCAGTTCACTGCATGCCTGCTGGATGTGGCATCCCCTGATGAGCCA GAACCCGGGGCCTCCAGGAGGGAAAAGAGGGACCAGCATATCCTGGCCCAGTTGCTTCCGCTGCTACATG GCAATGTGAATGGGAGCAAGGTGATCATCCATGAATTCCAGGAGCAATGCCGCAGGGGCCTGCTGACCCT TCCAAGTCCCACCCCACACCTGCAGATGCCCAACTTGGAGGATGCTGTTGCTGTGCCATCCAAGGCCAGG CTGAAGCGCCTTATCTCTGAGAACTCTGCCTATGAGAAGCGGCCCAACTTCCGTATGTGCTGGTATGTGC ACCCAGAGGTGCTCAAGAGCTTTGGCCAGGAGTGCCTGCCAGTGCCCTGCCAGTGGACCTACATCACCAC CATGCCCTCAGCACCCAGGGAAGACAGTGGCAGTGCTTCTACTGAGGGGCCTGGCCAGAGCACACCTATG CTACTCAAGCGGAAGCCAGCGGCCACCATGTGCATCACGCAGTTTATGAAGAAGCGGCGCTATGATGGAC AGGTTGGGTCTGGGGACATGGATGGCTTCCAGGCAGACACAGAAGAGGATGAAGAGGATGACACAGACTG TATGATCATAGATGTGCCAGATGTTGGGAGTGACGTGTCAGAAGCTCCCATACCTGCTCCCACACTTTGC AAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211155 representing NM_013733
Red=Cloning site Green=Tags(s) MLEEPEAATRTAAAVDCKDRPGFPVKRLIQARLPFKRLNLVPKEKVEEDTSPKAAVESKVPDLQLSLGTF ESQCHTGSHVGLSTKLVGGQGPIDSFLRATIKPVPSVVIIDLTENCSDIPDSPEGHSELSPDTAGVVTTV EGAAKQQEHSAAELCLLETPSDITCHMEEEPGSPGDPKRTGDCQAGSLQSCPELTPGSRTCPTKELSSWS KAGDLLFIEKVPVVVLEDILATKPSIASLPMMSLDRSVTSESEILESCPEDDSILSHSSTNSSSPTSSPE GPSTPPEHRGGRSSPSTPACRVAKNFVKGSTEKGRSKLHRDREQQREEKEKLREEIRRAKEEARKKKEEE KELKEKERREKREKDEKEKAEKQRLKEEKRKERQEALEAKLEEKRKKEEEKRLREEEKRLREEEKRIKAE KAEITRFFQKPKTPQAPKTLAGSCGKFAPFEIKEHMVLAPRCRAALDQDLCDQLDQLLQQQSVASTFLSD LKSRLPLRSGPTRVCGHDTDIMNRDVVIVESSKVDGVSERKKFGRMKLLQFSENHRPAYWGTWNKKTAII RPRNPWAQDKDLLDYEVDSDDEWEEEEPGESLSHSEGDEDDDVGEDEDEDDGFFVPHGYLSEDEGVTEEC ADPENHKVHQKLKAKEWDELLAKGKRFRVLQPVHVGCVWASEAANCTSSDLKLLQQFTACLLDVASPDEP EPGASRREKRDQHILAQLLPLLHGNVNGSKVIIHEFQEQCRRGLLTLPSPTPHLQMPNLEDAVAVPSKAR LKRLISENSAYEKRPNFRMCWYVHPEVLKSFGQECLPVPCQWTYITTMPSAPREDSGSASTEGPGQSTPM LLKRKPAATMCITQFMKKRRYDGQVGSGDMDGFQADTEEDEEDDTDCMIIDVPDVGSDVSEAPIPAPTLC K TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_013733 |
| ORF Size | 2733 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_013733.3, NP_038761.1 |
| RefSeq Size | 3308 bp |
| RefSeq ORF | 2736 bp |
| Locus ID | 27221 |
| UniProt ID | Q9QWF0 |
| MW | 101.9 kDa |
| Gene Summary | Core component of the CAF-1 complex, a complex thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. CHAF1A binds to histones H3 and H4. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222524 | Chaf1a (untagged) - Mouse chromatin assembly factor 1, subunit A (p150) (Chaf1a), (10ug) |
CNY 11,690.00 |
|
| MG211155 | Chaf1a (tGFP-tagged) - Mouse chromatin assembly factor 1, subunit A (p150) (Chaf1a) |
CNY 7,890.00 |
|
| MR211155L3 | Lenti ORF clone of Chaf1a (Myc-DDK-tagged) - Mouse chromatin assembly factor 1, subunit A (p150) (Chaf1a) |
CNY 9,030.00 |
|
| MR211155L4 | Lenti ORF clone of Chaf1a (mGFP-tagged) - Mouse chromatin assembly factor 1, subunit A (p150) (Chaf1a) |
CNY 9,030.00 |
