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Unc5b (BC057560) Mouse Tagged ORF Clone
CAT＃: MR211242
- TrueORF®
Unc5b (Myc-DDK-tagged) - Mouse unc-5 homolog B (C. elegans) (cDNA clone MGC:66787 IMAGE:6417563)
ORF Plasmid: tGFP
"BC057560" in other vectors (4)
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CNY 6,840.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 6330415E02Rik; A630020F16; D10Bwg0792e; Unc5h2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211242 representing BC057560
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGGCCCGGAGCGGGGTGCGGAGCGCGCTGCTGCTGGCGCTGCTGCTTTGCTGGGATCCGACACCGA GCCTAGCAGGCGTTGACTCTGCTGGCCAGGTGCTCCCAGACTCCTACCCATCAGCCCCTGCGGAGCAGCT GCCGTACTTCCTATTGGAGCCACAGGACGCCTACATCGTAAAGAACAAGCCAGTGGAACTGCACTGCAGA GCCTTCCCTGCCACGCAGATCTACTTCAAGTGTAATGGCGAGTGGGTCAGCCAGAATGACCACGTCACAC AGGAGAGCCTGGATGAGGCCACAGGCTTGCGGGTGCGAGAGGTGCAGATCGAGGTGTCACGGCAGCAAGT GGAGGAACTCTTCGGGCTCGAGGACTACTGGTGCCAGTGCGTGGCCTGGAGCTCTTCGGGAACTACCAAG AGTCGCCGAGCCTACATCCGCATTGCCTACTTGCGCAAGAACTTTGACCAGGAGCCTCTGGCCAAGGAGG TACCCTTGGATCATGAGGTCCTTCTGCAGTGCCGCCCACCGGAGGGAGTGCCTGTGGCTGAGGTGGAATG GCTCAAGAATGAAGATGTCATTGACCCCGCTCAGGACACTAACTTCCTGCTCACCATTGACCACAACCTC ATCATCCGCCAGGCGCGCCTCTCAGACACGGCCAACTACACCTGTGTGGCCAAGAATATCGTGGCCAAGC GCCGGAGCACCACGGCCACAGTCATCGTCTATGTGAATGGAGGCTGGTCCAGCTGGGCAGAGTGGTCACC CTGTTCCAATCGCTGTGGCCGAGGCTGGCAGAAGCGTACTCGGACCTGCACCAATCCAGCCCCACTCAAT GGAGGCGCCTTCTGTGAGGGACAGGCCTTCCAGAAGACAGCTTGCACCACCGTGTGCCCAGTGGATGGAG CGTGGACCGAGTGGAGCAAGTGGTCTGCCTGCAGCACAGAGTGTGCGCACTGGCGCAGCCGCGAGTGCAT GGCACCGCCACCCCAGAACGGAGGCCGTGACTGCAGCGGGACGCTACTTGACTCCAAGAACTGCACTGAT GGGCTGTGCGTGCTGACCCTGGAGACATCGGGAGATGTGGCACTGTACGCAGGCCTTGTGGTGGCCGTCT TTGTGGTGGTAGCGGTTCTCATGGCCGTGGGAGTGATCGTATACCGGAGAAACTGCCGGGACTTCGACAC GGACATCACCGACTCCTCTGCGGCCCTCACTGGTGGCTTCCACCCTGTCAACTTCAAGACTGCAAGGCCC AACAACCCGCAGCTCCTGCACCCGTCCGCCCCTCCAGACCTAACGGCCAGTGCTGGCATCTACCGCGGGC CTGTGTATGCCCTGCAGGACTCCGCCGACAAGATCCCCATGACTAATTCGCCCCTGCTGGATCCCCTGCC CAGCCTCAAGATCAAGGTCTATAACTCCAGCACCATCGGTTCTGGGTCTGGCCTGGCTGATGGAGCCGAC CTGCTGGGTGTCCTCCCGCCGGGCACGTACCCAGGCGATTTCTCCCGGGACACCCATTTCCTGCACCTGC GCAGTGCCAGCCTTGGTTCCCAGCACCTCCTGGGCCTACCTCGGGACCCCAGCAGCAGTGTCAGCGGCAC CTTTGGTTGCCTGGGAGGAAGGCTGAGCCTCCCCGGCACAGGGGTCAGCCTGTTGGTACCAAATGGAGCC ATTCCCCAGGGCAAGTTCTATGACCTGTATCTACATATCAACAAGGCCGAAAGCACCCTCCCACTTTCAG AAGGTTCCCAGACAGTATTGAGCCCCTCGGTGACCTGTGGGCCCACAGGCCTACTCCTGTGCCGCCCTGT CGTCCTCACCGTGCCCCACTGTGCTGAAGTCATCGCTGGAGACTGGATCTTTCAGCTCAAGACCCAGGCC CATCAGGGCCACTGGGAGGAGGTGGTGACCTTGGATGAGGAGACCCTCAACACACCCTGCTACTGCCAGC TGGAGGCTAAGTCCTGCCACATCCTGCTGGACCAGCTGGGTACCTACGTATTCATGGGCGAGTCCTACTC TCGCTCTGCAGTCAAGCGGCTCCAGCTGGCCATCTTCGCCCCAGCCCTCTGCACCTCCCTGGAGTATAGC CTCAGGGTCTACTGTCTGGAGGACACACCTGTAGCACTGAAGGAGGTCCTGGAGCTGGAGAGGACTCTGG GTGGCTACTTGGTGGAGGAGCCCAAGCCTTTGCTCTTTAAGGACAGTTACCACAACCTACGCCTCTCCCT CCATGACATCCCCCATGCCCACTGGAGGAGCAAACTACTGGCCAAGTACCAGGAGATTCCCTTCTACCAC GTCTGGAATGGCAGCCAGAGAGCCCTGCACTGCACTTTCACCCTGGAGAGGCATAGCCTGGCCTCCACGG AGTTCACCTGTAAGGTCTGCGTGCGGCAGGTCGAAGGGGAAGGCCAGATTTTCCAGCTGCACACAACGTT GGCCGAGACGCCTGCTGGCTCCCTGGATGCTCTCTGCTCTGCCCCGGGCAATGCCATCACCACCCAGCTG GGACCCTATGCCTTCAAGATACCCCTGTCCATCCGCCAAAAGATCTGCAGCAGCCTGGACGCCCCCAACT CCCGGGGCAACGACTGGAGGCTGTTGGCGCAGAAGCTGTCCATGGACCGGTACCTAAACTACTTCGCCAC CAAAGCTAGTCCCACAGGTGTCATCTTAGACCTCTGGGAAGCTCGGCAACAGGATGACGGGGACCTCAAC AGCCTGGCCAGTGCCTTGGAGGAGATGGGCAAGAGTGAGATGCTGGTAGCCATGGCCACAGATGGCGATT GC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211242 representing BC057560
Red=Cloning site Green=Tags(s) MRARSGVRSALLLALLLCWDPTPSLAGVDSAGQVLPDSYPSAPAEQLPYFLLEPQDAYIVKNKPVELHCR AFPATQIYFKCNGEWVSQNDHVTQESLDEATGLRVREVQIEVSRQQVEELFGLEDYWCQCVAWSSSGTTK SRRAYIRIAYLRKNFDQEPLAKEVPLDHEVLLQCRPPEGVPVAEVEWLKNEDVIDPAQDTNFLLTIDHNL IIRQARLSDTANYTCVAKNIVAKRRSTTATVIVYVNGGWSSWAEWSPCSNRCGRGWQKRTRTCTNPAPLN GGAFCEGQAFQKTACTTVCPVDGAWTEWSKWSACSTECAHWRSRECMAPPPQNGGRDCSGTLLDSKNCTD GLCVLTLETSGDVALYAGLVVAVFVVVAVLMAVGVIVYRRNCRDFDTDITDSSAALTGGFHPVNFKTARP NNPQLLHPSAPPDLTASAGIYRGPVYALQDSADKIPMTNSPLLDPLPSLKIKVYNSSTIGSGSGLADGAD LLGVLPPGTYPGDFSRDTHFLHLRSASLGSQHLLGLPRDPSSSVSGTFGCLGGRLSLPGTGVSLLVPNGA IPQGKFYDLYLHINKAESTLPLSEGSQTVLSPSVTCGPTGLLLCRPVVLTVPHCAEVIAGDWIFQLKTQA HQGHWEEVVTLDEETLNTPCYCQLEAKSCHILLDQLGTYVFMGESYSRSAVKRLQLAIFAPALCTSLEYS LRVYCLEDTPVALKEVLELERTLGGYLVEEPKPLLFKDSYHNLRLSLHDIPHAHWRSKLLAKYQEIPFYH VWNGSQRALHCTFTLERHSLASTEFTCKVCVRQVEGEGQIFQLHTTLAETPAGSLDALCSAPGNAITTQL GPYAFKIPLSIRQKICSSLDAPNSRGNDWRLLAQKLSMDRYLNYFATKASPTGVILDLWEARQQDDGDLN SLASALEEMGKSEMLVAMATDGDC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC057560 |
| ORF Size | 2802 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC057560.1 |
| RefSeq Size | 3672 bp |
| RefSeq ORF | 2804 bp |
| Locus ID | 107449 |
| MW | 134.6 kDa |
| Gene Summary | Receptor for netrin required for axon guidance. Mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding. Axon repulsion in growth cones may be caused by its association with DCC that may trigger signaling for repulsion (By similarity). Functions as netrin receptor that negatively regulates vascular branching during angiogenesis (PubMed:15510105). Mediates retraction of tip cell filopodia on endothelial growth cones in response to netrin (PubMed:15510105). It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand. Mediates apoptosis by activating DAPK1. In the absence of NTN1, activates DAPK1 by reducing its autoinhibitory phosphorylation at Ser-308 thereby increasing its catalytic activity (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220874 | Unc5b (untagged) - Mouse unc-5 homolog B (C. elegans) (cDNA clone MGC:66787 IMAGE:6417563), (10ug) |
CNY 9,120.00 |
|
| MG211242 | Unc5b (tGFP-tagged) - Mouse unc-5 homolog B (C. elegans) (cDNA clone MGC:66787 IMAGE:6417563) |
CNY 5,890.00 |
|
| MR211242L3 | Lenti ORF clone of Unc5b (Myc-DDK-tagged) - Mouse unc-5 homolog B (C. elegans) (cDNA clone MGC:66787 IMAGE:6417563) |
CNY 7,220.00 |
|
| MR211242L4 | Lenti ORF clone of Unc5b (mGFP-tagged) - Mouse unc-5 homolog B (C. elegans) (cDNA clone MGC:66787 IMAGE:6417563) |
CNY 7,220.00 |
