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Vps54 (BC025012) Mouse Tagged ORF Clone
CAT＃: MR211333
- TrueORF®
Vps54 (Myc-DDK-tagged) - Mouse vacuolar protein sorting 54 (yeast) (cDNA clone MGC:32446 IMAGE:5042879)
ORF Plasmid: tGFP
"BC025012" in other vectors (4)
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CNY 9,600.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Vps54l, Hcc8 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211333 representing BC025012
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTCAAGCCACAGTTCTTCTCCAGTGCCTCAAGGAAGCAGCAGTGATGTTTTCTTTAAAAAAGAGG TAGATCCAACAAAACACATTCGACCTGTGCAGTCACTGCCAGATGTGTGTCCCAAGGAACCCACAGTTAC AGATCAACATAGATGGACTGTGTATCATTCCAAAGTAAATCTCCCAGCAGCATTAAATGATCCTACACTA GCAAAAAGAGAATCTGACTTCTTCACAAAAACTTGGGGATTGGACTTTGTGGACACTGAAGTCATACCTT CACTCTACCTCCCACAGATCAGCAAGGAGAATTTTATAGCATATCAGCAGGAGATCTCTCAGAGAGAGAA GATTCATGAGAGATGCAAGAATATTTGTCCTCCTAAAGATACCTTTGACAGGACTCTTTTACATATTCAT GATAAATCCAGGACAGATCTGGAGCAAGTACCTAAGATTTTTATGAAACCAGATTTTGCCTTGGATGATT CTTTAACTTTTAATTCAGTTTTACCATGGTCTCATTTTAATACTGCTGGTGGGAAAGGAAGTCGCGACGC AGCTTCTTCCAAGTTGCTTCAGGAAAAGCTGAGCCATTATCTGGATATTGTGGAAGTAAACATTGCTCAC CAGATCTCTCTACGTTCAGAAGCATTTTTTCATGCAATGACCTCTCAACACGAATTGCAGGACTACCTCA AGAAAACTACCCAGGCTGTAAAAATGCTTCGAGATAAAATTGCACAGATTGATAAAGTTATGTGTGAAGG ATCACTACAGATTTTAAGACTGGCACTTACCAGAAATAATTGTGTTAAAGTATACAATAAACTGAAGTTA ATGGCCACTGTACACCAAACTCAGCCTACAGTGCAGGTGTTGTTATCTACTTCTGAATTTGTTGGAGCGT TGGACTTAATAGCAACAACTCAAGAGGTTCTGCAGCAGGAGCTTCAGGGCATTCACAGCTTCCGGCATTT GGGATCACAGCTTTGTGAATTAGAAAAGCTGATTGATAAAATGATGATTGCAGAATTTTCTACTTATTCT CATAGTGACTTAAATAGACCACTGGAAGGTGAATGCCAAGTTTTAGAAGAGGAAAGATTAGTATCTCTTG TATTTGGGCTTTTGAAACAAAGAAAACTTAATTTTTTAGAAATCTATGGTGAAGAAATGATTATTACAGC AAAGAACATCATTAAAGAGCGTGTGATTAATAAAGTTTCACAAATAGAAGAAATAGACACAGACGTTGTT GTAAAGCTTGCAGATCAAATGAGAATGTTGAATTTTCCCCAGTGGATTGATCTGTTAAAGGACATTTTCT CTAAGTTTACAGTTTTCCTACAGAGAGTTAAGGCAACATTGAATATTATCCACAGTGTTGTTCTGTCAGT TCTTGAGAAAAGCCAAAGGACTAGAGAATTGGAAGAGATCCCACAGCAGAGGAGCGCTGGGAAGGACAGC TCCTTGGACACAGACGTGGCTTACCTGACACACGAGGGCTGGTTCATAAGTGATGCTTTCAGTGAGGGTG AGCCAGCATCTGCGGCGGTTGATACTACCTCTCAAAGAAACACGTCTCCACATAGCGAGCCCTGCAGCAG TGATTCGGTATCAGAACCAGAATGTACTACTGATTCTTCATCCAGCAAGGAGCAGACATCAGCGTGTGCT CCTCCAGGAGGTATAGAGATTATAGTCAGTGAAGACATGAGGTTAACTGACTTGGAGCTAGGAAAACTAG CAAGTAACATCCAGGAGTTACTGTGTAATGCTTCAGATGTATGCCATGATCGAGCTGTCAAATTTCTCAT GTCAAGAGCAAAGGACGGTTTTCTTGAAAAATTGAATTCCACGGAATTCATAGCACTCTCGAGATTAATG GAGACATTCATAGTGGACACTGAACAGATCTGTGGGAGGAAGAGCACGTCACTGCTTGGGGCCCTTCAGA GCCAAGCAAATAAGTTTGTGAACAGGTTTCATGAAGAGAGGCGGACCAAGCTCAGCCTCCTCTTAGACAA CGAGCGTTGGAAGCAGGCTGATGTTCCTGCAGAATTTCAGGATCTTGTTGATTCTATAGCGGATGGAAAG ATTGCTTTACCTGAAAAAAAGCCAGTGGTTACAGAAGAAAGGAAGCCAGCTGATGTTCTTGTTGTTGAGG GACACCAGTATGCAGTTGTCGGAACCGTCTTGCTGTTAATAAGAATCATTCTTGAATATTGCCAGTGTGT GGATAACATCCCCTCCGTCACTACTGACATGCTCACTCGCCTGACAGATCTGCTGAAGTACTTCAATTCA AGAAGTTGCCAGTTAGTTCTTGGAGCTGGTGCTTTGCAAGTTGTTGGACTAAAAACAATTACTACAAAAA ACTTGGCCCTCTCTTCACGGTGTCTGCAGTTGATTGTGCACTACATTCCCGTGATCCGGGCTCACTTTGA AGCCCGACTGCCCCCCAAGCAATGGAGCATGCTTAGACATTTTGATCACATCACAAAGGATTACCATGAT CATATAGCTGAAATTTCAGCTAAGCTTGTAGCGATAATGGATAGCCTATTTGATAAGCTGTTATCAAAGT ATGAGGTGAAAGCTCCTGTTCCTTCCCCGTGTTTCAGGAATATTTGTAAGCAAATGACAAAAATGCATGA AGCTATATTTGATCTTCTTCCCGAAGAACAAACACAGATGTTATTTTTAAGAATTAATGCAAGTTACAAA CTCCACTTGAAAAAACAGTTATCACACTTAAATGTAATAAATGACGGAGGACCTCAAAATGGGTTGGTCA CAGCAGATGTAGCTTTTTACACTGGAAATCTTCAAGCCTTAAAAGGCCTTAAAGATCTGGATCTAAATAT GGCCGAGATCTGGGAACAGAAGAGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211333 representing BC025012
Red=Cloning site Green=Tags(s) MASSHSSSPVPQGSSSDVFFKKEVDPTKHIRPVQSLPDVCPKEPTVTDQHRWTVYHSKVNLPAALNDPTL AKRESDFFTKTWGLDFVDTEVIPSLYLPQISKENFIAYQQEISQREKIHERCKNICPPKDTFDRTLLHIH DKSRTDLEQVPKIFMKPDFALDDSLTFNSVLPWSHFNTAGGKGSRDAASSKLLQEKLSHYLDIVEVNIAH QISLRSEAFFHAMTSQHELQDYLKKTTQAVKMLRDKIAQIDKVMCEGSLQILRLALTRNNCVKVYNKLKL MATVHQTQPTVQVLLSTSEFVGALDLIATTQEVLQQELQGIHSFRHLGSQLCELEKLIDKMMIAEFSTYS HSDLNRPLEGECQVLEEERLVSLVFGLLKQRKLNFLEIYGEEMIITAKNIIKERVINKVSQIEEIDTDVV VKLADQMRMLNFPQWIDLLKDIFSKFTVFLQRVKATLNIIHSVVLSVLEKSQRTRELEEIPQQRSAGKDS SLDTDVAYLTHEGWFISDAFSEGEPASAAVDTTSQRNTSPHSEPCSSDSVSEPECTTDSSSSKEQTSACA PPGGIEIIVSEDMRLTDLELGKLASNIQELLCNASDVCHDRAVKFLMSRAKDGFLEKLNSTEFIALSRLM ETFIVDTEQICGRKSTSLLGALQSQANKFVNRFHEERRTKLSLLLDNERWKQADVPAEFQDLVDSIADGK IALPEKKPVVTEERKPADVLVVEGHQYAVVGTVLLLIRIILEYCQCVDNIPSVTTDMLTRLTDLLKYFNS RSCQLVLGAGALQVVGLKTITTKNLALSSRCLQLIVHYIPVIRAHFEARLPPKQWSMLRHFDHITKDYHD HIAEISAKLVAIMDSLFDKLLSKYEVKAPVPSPCFRNICKQMTKMHEAIFDLLPEEQTQMLFLRINASYK LHLKKQLSHLNVINDGGPQNGLVTADVAFYTGNLQALKGLKDLDLNMAEIWEQKR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC025012 |
| ORF Size | 2895 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC025012.2 |
| RefSeq Size | 3405 bp |
| RefSeq ORF | 2897 bp |
| Locus ID | 245944 |
| MW | 124.9 kDa |
| Gene Summary | Acts as component of the GARP complex that is involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN). The GARP complex is required for the maintenance of the cycling of mannose 6-phosphate receptors between the TGN and endosomes, this cycling is necessary for proper lysosomal sorting of acid hydrolases such as CTSD. Within the GARP complex, required to tether the complex to the TGN. Not involved in endocytic recycling.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220936 | Vps54 (untagged) - Mouse vacuolar protein sorting 54 (yeast) (cDNA clone MGC:32446 IMAGE:5042879), (10ug) |
CNY 9,120.00 |
|
| MG211333 | Vps54 (tGFP-tagged) - Mouse vacuolar protein sorting 54 (yeast) (cDNA clone MGC:32446 IMAGE:5042879) |
CNY 10,550.00 |
|
| MR211333L3 | Lenti ORF clone of Vps54 (Myc-DDK-tagged) - Mouse vacuolar protein sorting 54 (yeast) (cDNA clone MGC:32446 IMAGE:5042879) |
CNY 11,500.00 |
|
| MR211333L4 | Lenti ORF clone of Vps54 (mGFP-tagged) - Mouse vacuolar protein sorting 54 (yeast) (cDNA clone MGC:32446 IMAGE:5042879) |
CNY 11,500.00 |
