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Ppp1r9a (BC053748) Mouse Tagged ORF Clone
CAT＃: MR211345
- TrueORF®
Ppp1r9a (Myc-DDK-tagged) - Mouse protein phosphatase 1, regulatory (inhibitor) subunit 9A (cDNA clone MGC:59493 IMAGE:6331919)
ORF Plasmid: tGFP
"BC053748" in other vectors (4)
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CNY 7,096.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 5330407E15, mKIAA1222, A230094E16Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211345 representing BC053748
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGAAGGCGGAATCTTCAGGGGAGCGGACCACTCTCAGAAGTGCCTCTCCTCACAGGAATGCGTACA GAACAGAGTTCCAGGCACTGAAAAGTACTTTTGACAAACCCAAGTCAGATGGGGAACAGAAAACGAAAGA AGGTGAGGGCTCCCAGCAGAGCAGAGGTAGAAAGTATGGCTCCAATGTCAACAGAATTAAAAATCTATTT ATGCAGATGGGTATGGAACCCAGCGAGAATGCTGCTATCATTGCCAAAACCAGGGGGAAAGGTAGGCCCT CGTCCCCTCAGAGAAGAATGAAGCCCAAAGAGTTTGTGGAGAAAACGGATGGCTCAGTGGTTAAGTTGGA GTCCTCTGTCTCTGAACGAATCAGTAGATTTGACACAATGCATGATGGCCCTTCGTATGCTAAGTTCACG GAGACTCGAAAGATGTTTGAGAGAAGTGTGCATGAGTCAGGGCAAAACCATCGCTATTCCCCAAAGAAAG AGAAAGGTGGAGGGACTGAACCACAGGATGAGTGGGGTGGCTCCAAGTCCAACAGAGGCAGCAGTGATTC CTTGGACAGCCTGAGCCCCCGGACGGAAGCGGTCTCTCCTACTGTGAGTCAGCTGAGTGCTGTGTTTGAG AACAGTGAGCCTCCAGGTGCTCTCACTTCTGGGAAGGCTGAGAGCGCTGACTACTCGGTGACCGGGCACT ACCCCCTCAATTTACCATCTGTTACCGTCACCAATCTGGATACCTTTGGCCGTCTCAAGGATTCTAATTC GAAGCCTCCATCAAACAAACAAGATACTGATACAGAGGATGCTCAGAAGAGCGATGCTGTGCCAGTACCG GAAGTGGCTCAGAAAGGTACCTCTTTAGCTTCATTGCCTAGTGAAGAGATCCAGCTGAGCACGGAAGCCG AGGACGTCACAACAGCTCAACCAGAGGCTCTGGACAGCACTGATAAAGACAGTCCTGAGGAACCCTCTGC TGAAAACCAGGCAATGCCAAAGTCTCATATCCTTTCCCCACGGAACGAGCCGTTAGAAGATGCTGAAGCT AATGTGGTTGGGAGTGAGAGAGCTCAGCATCAGAAGAAAGACCTGACAGGTGGTGACCTAACCTCCCCTG ATGCTTCTGCATCCAGCTGTGGAAGAGAAGTACCTGAAGACTCAAACAGTTTTGAGAGTTCCCACGTATA CATGCATAGTGACTATAACGTGTATAGGGTGAGATCCAGGTATAACTCAGACTGGGGAGAGACAGGCACG GAGCAAGATGAGGAGGAAGACAGTGACGAGAACAATTATTACCAGCCTGATATGGAATACTCGGAGATAG TTGGCTTGCCGGAAGAAGAAGAAATCCCAGCAAATAGGAAAATTAAGTTTAGTTGTGCTCCGATTAAGGT TTTCAACACATACTCCAATGAAGACTATGACCGGAGAAATGACGACGTTGACCCTGTAGCCGCATCTGCT GAGTATGAGCTTGAAAAGCGAGTAGAAAAACTGGAACTTTTCCCAGTGGAACTGGAGAAAGACGAGGACG GGCTGGGCATAAGCATCATTGGAATGGGCGTTGGTGCTGATGCTGGATTGGAGAAGCTGGGGATATTTGT CAAGACAGTGACTGACGGAGGTGCTGCTCAGCGGGATGGCAGGATTCAAGTCAATGACCAGATTGTGGAA GTTGATGGAATCAGCCTGGTGGGGGTCACACAGAATTTTGCTGCAACCGTTCTGCGGAATACCAAGGGCA ACGTCAGATTCGTTATTGGACGAGAAAAACCAGGACAAGTGAGTGAGGTTGCCCAGTTGATCAGCCAGAC CCTGGAACAAGAGCGGCGACAGCGGGAGCTTCTGGAGCGTCACTATGCCCAGTATGATGCTGACGACGAT GAGACTGGAGAGTATGCCACTGATGAAGAAGAGGATGAGGGCGGGCCTATTCTTCCTAGTGGTGACGTGG CCATTGAGGTCTTTGAGCTGCCTGAGAACGAGGACATGTTTTCCCCATCTGACCTGGACACAAGCAAGCT CAGCCACAAGTTCAAAGAGTTGCAAATCAAACATGCAGTTACAGAAGCAGAGATTCAAAAATTGAAGACC AAGCTTCAAGCAGCCGAAAATGAGAAAGTAAGGTGGGAACTAGAAAAGAACCAACTGCAACAGAATATAG AAGAGAATAAAGAACGGATGCTGAAGTTGGAGAGCTACTGGATCGAGGCTCAGACATTATGTCATACGGT GAATGAGCATCTCAAAGAGACTCAGAGCCAGTACCAAGCCCTGGAAAAGAAATACAACAAAGCAAAGAAG CTGATCAAAGACTTCCAGCAAAAAGAGCTCGATTTCATCAAGAGACAGGAAGTAGAAAGAAAGAAGCGGG AGGAGGTGGAAAAGGCTCACCTGCTTGAAGTCCAAGGCCTGCAAGTTCGGATTAGAGATTTGGAGGCTGA GGTGTTCAGACTACTAAAGCAAAATGGGACCCAGGTTAACAACAACAACAACATCTTTGAGAGAAGACCA TCTCCCGGGGAAGTCTCGAAAGGAGACACTATGGAGAATGTGGAAGTCAAGCAAACATCCTGTCAGGACG GCTTGAGCCAAGACCTGAATGAAGCAGTCCCAGAGACAGAGCGCCTGGATTCGAAAGCATTGAAAACCCG GGCCCAGCTCTCTGTGAAGAACAGGCGCCAGAGGCCCACAAGGACACGGCTCTATGACAGCGTCAGCTCA ACTGATGGGGAGGACAGCCTGGAGAGGAAGGTGAGCACTCTCAATGGCTGGCAGACTCTTGCAGAGTGTC GTTGTCCACCAGTGTATTTATTGAACGTGATAGCGGTTTTACTGATCTGTGCCTTACTTGGAAGAAAGTC TCCTAAATTTGTATTCAATTGCTATATGGTTGTCATT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211345 representing BC053748
Red=Cloning site Green=Tags(s) MLKAESSGERTTLRSASPHRNAYRTEFQALKSTFDKPKSDGEQKTKEGEGSQQSRGRKYGSNVNRIKNLF MQMGMEPSENAAIIAKTRGKGRPSSPQRRMKPKEFVEKTDGSVVKLESSVSERISRFDTMHDGPSYAKFT ETRKMFERSVHESGQNHRYSPKKEKGGGTEPQDEWGGSKSNRGSSDSLDSLSPRTEAVSPTVSQLSAVFE NSEPPGALTSGKAESADYSVTGHYPLNLPSVTVTNLDTFGRLKDSNSKPPSNKQDTDTEDAQKSDAVPVP EVAQKGTSLASLPSEEIQLSTEAEDVTTAQPEALDSTDKDSPEEPSAENQAMPKSHILSPRNEPLEDAEA NVVGSERAQHQKKDLTGGDLTSPDASASSCGREVPEDSNSFESSHVYMHSDYNVYRVRSRYNSDWGETGT EQDEEEDSDENNYYQPDMEYSEIVGLPEEEEIPANRKIKFSCAPIKVFNTYSNEDYDRRNDDVDPVAASA EYELEKRVEKLELFPVELEKDEDGLGISIIGMGVGADAGLEKLGIFVKTVTDGGAAQRDGRIQVNDQIVE VDGISLVGVTQNFAATVLRNTKGNVRFVIGREKPGQVSEVAQLISQTLEQERRQRELLERHYAQYDADDD ETGEYATDEEEDEGGPILPSGDVAIEVFELPENEDMFSPSDLDTSKLSHKFKELQIKHAVTEAEIQKLKT KLQAAENEKVRWELEKNQLQQNIEENKERMLKLESYWIEAQTLCHTVNEHLKETQSQYQALEKKYNKAKK LIKDFQQKELDFIKRQEVERKKREEVEKAHLLEVQGLQVRIRDLEAEVFRLLKQNGTQVNNNNNIFERRP SPGEVSKGDTMENVEVKQTSCQDGLSQDLNEAVPETERLDSKALKTRAQLSVKNRRQRPTRTRLYDSVSS TDGEDSLERKVSTLNGWQTLAECRCPPVYLLNVIAVLLICALLGRKSPKFVFNCYMVVI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC053748 |
| ORF Size | 2907 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC053748.1 |
| RefSeq Size | 9473 bp |
| RefSeq ORF | 2909 bp |
| Locus ID | 243725 |
| MW | 347.3 kDa |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220931 | Ppp1r9a (untagged) - Mouse protein phosphatase 1, regulatory (inhibitor) subunit 9A (cDNA clone MGC:59493 IMAGE:6331919), (10ug) |
CNY 9,120.00 |
|
| MG211345 | Ppp1r9a (tGFP-tagged) - Mouse protein phosphatase 1, regulatory (inhibitor) subunit 9A (cDNA clone MGC:59493 IMAGE:6331919) |
CNY 6,080.00 |
|
| MR211345L3 | Lenti ORF clone of Ppp1r9a (Myc-DDK-tagged) - Mouse protein phosphatase 1, regulatory (inhibitor) subunit 9A (cDNA clone MGC:59493 IMAGE:6331919) |
CNY 7,410.00 |
|
| MR211345L4 | Lenti ORF clone of Ppp1r9a (mGFP-tagged) - Mouse protein phosphatase 1, regulatory (inhibitor) subunit 9A (cDNA clone MGC:59493 IMAGE:6331919) |
CNY 7,410.00 |
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