Ppp1r9a (BC053748) Mouse Tagged ORF Clone

CAT#: MR211345

  • TrueORF®

Ppp1r9a (Myc-DDK-tagged) - Mouse protein phosphatase 1, regulatory (inhibitor) subunit 9A (cDNA clone MGC:59493 IMAGE:6331919)

ORF Plasmid: DDK tGFP



  "BC053748" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 7,096.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms 5330407E15, mKIAA1222, A230094E16Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR211345 representing BC053748
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTGAAGGCGGAATCTTCAGGGGAGCGGACCACTCTCAGAAGTGCCTCTCCTCACAGGAATGCGTACA
GAACAGAGTTCCAGGCACTGAAAAGTACTTTTGACAAACCCAAGTCAGATGGGGAACAGAAAACGAAAGA
AGGTGAGGGCTCCCAGCAGAGCAGAGGTAGAAAGTATGGCTCCAATGTCAACAGAATTAAAAATCTATTT
ATGCAGATGGGTATGGAACCCAGCGAGAATGCTGCTATCATTGCCAAAACCAGGGGGAAAGGTAGGCCCT
CGTCCCCTCAGAGAAGAATGAAGCCCAAAGAGTTTGTGGAGAAAACGGATGGCTCAGTGGTTAAGTTGGA
GTCCTCTGTCTCTGAACGAATCAGTAGATTTGACACAATGCATGATGGCCCTTCGTATGCTAAGTTCACG
GAGACTCGAAAGATGTTTGAGAGAAGTGTGCATGAGTCAGGGCAAAACCATCGCTATTCCCCAAAGAAAG
AGAAAGGTGGAGGGACTGAACCACAGGATGAGTGGGGTGGCTCCAAGTCCAACAGAGGCAGCAGTGATTC
CTTGGACAGCCTGAGCCCCCGGACGGAAGCGGTCTCTCCTACTGTGAGTCAGCTGAGTGCTGTGTTTGAG
AACAGTGAGCCTCCAGGTGCTCTCACTTCTGGGAAGGCTGAGAGCGCTGACTACTCGGTGACCGGGCACT
ACCCCCTCAATTTACCATCTGTTACCGTCACCAATCTGGATACCTTTGGCCGTCTCAAGGATTCTAATTC
GAAGCCTCCATCAAACAAACAAGATACTGATACAGAGGATGCTCAGAAGAGCGATGCTGTGCCAGTACCG
GAAGTGGCTCAGAAAGGTACCTCTTTAGCTTCATTGCCTAGTGAAGAGATCCAGCTGAGCACGGAAGCCG
AGGACGTCACAACAGCTCAACCAGAGGCTCTGGACAGCACTGATAAAGACAGTCCTGAGGAACCCTCTGC
TGAAAACCAGGCAATGCCAAAGTCTCATATCCTTTCCCCACGGAACGAGCCGTTAGAAGATGCTGAAGCT
AATGTGGTTGGGAGTGAGAGAGCTCAGCATCAGAAGAAAGACCTGACAGGTGGTGACCTAACCTCCCCTG
ATGCTTCTGCATCCAGCTGTGGAAGAGAAGTACCTGAAGACTCAAACAGTTTTGAGAGTTCCCACGTATA
CATGCATAGTGACTATAACGTGTATAGGGTGAGATCCAGGTATAACTCAGACTGGGGAGAGACAGGCACG
GAGCAAGATGAGGAGGAAGACAGTGACGAGAACAATTATTACCAGCCTGATATGGAATACTCGGAGATAG
TTGGCTTGCCGGAAGAAGAAGAAATCCCAGCAAATAGGAAAATTAAGTTTAGTTGTGCTCCGATTAAGGT
TTTCAACACATACTCCAATGAAGACTATGACCGGAGAAATGACGACGTTGACCCTGTAGCCGCATCTGCT
GAGTATGAGCTTGAAAAGCGAGTAGAAAAACTGGAACTTTTCCCAGTGGAACTGGAGAAAGACGAGGACG
GGCTGGGCATAAGCATCATTGGAATGGGCGTTGGTGCTGATGCTGGATTGGAGAAGCTGGGGATATTTGT
CAAGACAGTGACTGACGGAGGTGCTGCTCAGCGGGATGGCAGGATTCAAGTCAATGACCAGATTGTGGAA
GTTGATGGAATCAGCCTGGTGGGGGTCACACAGAATTTTGCTGCAACCGTTCTGCGGAATACCAAGGGCA
ACGTCAGATTCGTTATTGGACGAGAAAAACCAGGACAAGTGAGTGAGGTTGCCCAGTTGATCAGCCAGAC
CCTGGAACAAGAGCGGCGACAGCGGGAGCTTCTGGAGCGTCACTATGCCCAGTATGATGCTGACGACGAT
GAGACTGGAGAGTATGCCACTGATGAAGAAGAGGATGAGGGCGGGCCTATTCTTCCTAGTGGTGACGTGG
CCATTGAGGTCTTTGAGCTGCCTGAGAACGAGGACATGTTTTCCCCATCTGACCTGGACACAAGCAAGCT
CAGCCACAAGTTCAAAGAGTTGCAAATCAAACATGCAGTTACAGAAGCAGAGATTCAAAAATTGAAGACC
AAGCTTCAAGCAGCCGAAAATGAGAAAGTAAGGTGGGAACTAGAAAAGAACCAACTGCAACAGAATATAG
AAGAGAATAAAGAACGGATGCTGAAGTTGGAGAGCTACTGGATCGAGGCTCAGACATTATGTCATACGGT
GAATGAGCATCTCAAAGAGACTCAGAGCCAGTACCAAGCCCTGGAAAAGAAATACAACAAAGCAAAGAAG
CTGATCAAAGACTTCCAGCAAAAAGAGCTCGATTTCATCAAGAGACAGGAAGTAGAAAGAAAGAAGCGGG
AGGAGGTGGAAAAGGCTCACCTGCTTGAAGTCCAAGGCCTGCAAGTTCGGATTAGAGATTTGGAGGCTGA
GGTGTTCAGACTACTAAAGCAAAATGGGACCCAGGTTAACAACAACAACAACATCTTTGAGAGAAGACCA
TCTCCCGGGGAAGTCTCGAAAGGAGACACTATGGAGAATGTGGAAGTCAAGCAAACATCCTGTCAGGACG
GCTTGAGCCAAGACCTGAATGAAGCAGTCCCAGAGACAGAGCGCCTGGATTCGAAAGCATTGAAAACCCG
GGCCCAGCTCTCTGTGAAGAACAGGCGCCAGAGGCCCACAAGGACACGGCTCTATGACAGCGTCAGCTCA
ACTGATGGGGAGGACAGCCTGGAGAGGAAGGTGAGCACTCTCAATGGCTGGCAGACTCTTGCAGAGTGTC
GTTGTCCACCAGTGTATTTATTGAACGTGATAGCGGTTTTACTGATCTGTGCCTTACTTGGAAGAAAGTC
TCCTAAATTTGTATTCAATTGCTATATGGTTGTCATT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR211345 representing BC053748
Red=Cloning site Green=Tags(s)

MLKAESSGERTTLRSASPHRNAYRTEFQALKSTFDKPKSDGEQKTKEGEGSQQSRGRKYGSNVNRIKNLF
MQMGMEPSENAAIIAKTRGKGRPSSPQRRMKPKEFVEKTDGSVVKLESSVSERISRFDTMHDGPSYAKFT
ETRKMFERSVHESGQNHRYSPKKEKGGGTEPQDEWGGSKSNRGSSDSLDSLSPRTEAVSPTVSQLSAVFE
NSEPPGALTSGKAESADYSVTGHYPLNLPSVTVTNLDTFGRLKDSNSKPPSNKQDTDTEDAQKSDAVPVP
EVAQKGTSLASLPSEEIQLSTEAEDVTTAQPEALDSTDKDSPEEPSAENQAMPKSHILSPRNEPLEDAEA
NVVGSERAQHQKKDLTGGDLTSPDASASSCGREVPEDSNSFESSHVYMHSDYNVYRVRSRYNSDWGETGT
EQDEEEDSDENNYYQPDMEYSEIVGLPEEEEIPANRKIKFSCAPIKVFNTYSNEDYDRRNDDVDPVAASA
EYELEKRVEKLELFPVELEKDEDGLGISIIGMGVGADAGLEKLGIFVKTVTDGGAAQRDGRIQVNDQIVE
VDGISLVGVTQNFAATVLRNTKGNVRFVIGREKPGQVSEVAQLISQTLEQERRQRELLERHYAQYDADDD
ETGEYATDEEEDEGGPILPSGDVAIEVFELPENEDMFSPSDLDTSKLSHKFKELQIKHAVTEAEIQKLKT
KLQAAENEKVRWELEKNQLQQNIEENKERMLKLESYWIEAQTLCHTVNEHLKETQSQYQALEKKYNKAKK
LIKDFQQKELDFIKRQEVERKKREEVEKAHLLEVQGLQVRIRDLEAEVFRLLKQNGTQVNNNNNIFERRP
SPGEVSKGDTMENVEVKQTSCQDGLSQDLNEAVPETERLDSKALKTRAQLSVKNRRQRPTRTRLYDSVSS
TDGEDSLERKVSTLNGWQTLAECRCPPVYLLNVIAVLLICALLGRKSPKFVFNCYMVVI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC053748
ORF Size 2907 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC053748.1
RefSeq Size 9473 bp
RefSeq ORF 2909 bp
Locus ID 243725
MW 347.3 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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