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Jmy (NM_021310) Mouse Tagged ORF Clone
CAT＃: MR211383
- TrueORF®
Jmy (Myc-DDK-tagged) - Mouse junction-mediating and regulatory protein (Jmy)
ORF Plasmid: tGFP
"NM_021310" in other vectors (4)
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CNY 7,200.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AA591059 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211383 representing NM_021310
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGTTCGCGCTGGAGGAGACACTCGAGTCCGACTGGGTGGCGGTGCGGCCCCACGTATTCGACGAGC GCGAGAAGCACAAGTTTGTGTTCATTGTGGCCTGGAACGAGATCGAAGGCAAGTTTGCTATAACCTGTCA CAACCGGACGGCCCAGAGACAGAGGAGCGGTTCCCGGGAACAGGCGGGGACGCCCGCGTCTGATGGGAGT CGCGGTCCGGGCAGCCCCGCGGCCAGGGGTCGGTCAGAGGCCGCTGCCTCTGCTACAGCAGCGCTCCGGA GTCCCGGGCCACGGAAAAGCCAGGCCTGGGCCGAGGGCGGCTCTCCGCGCAGCGCGCGCAGCCTGAAGGG GGATCCTCCTCGGGGTCCCGCGGGCAGAGGACCGGAGAGTCCTCTCCGTAGCCCCGCGCGGGCTAAGGCC AGCCCGCTCCGCAGAAGCGCCGAATCCCGAGATGCGACCGCCAGTGCCACGCCAGCCCCGCCGGCGCCCC CGGTGCCCCCGGTGTCGTCGGTGCGGGTGGTGAGTGCCTCCGGGGCGGTCTCCGAGGAGATCGAGGTCCT GGAAATGGTGAGGGAGGACGAGGCGCCACAGCCGCTCCCGGACTCGGAGCAGCCGCCGTCTGCCGCGGAG CTGGAGTCTCCGGCCGAAGAATGCAGCTGGGCCGGGCTTTTCTCCTTCCAGGATCTGCGAGCCGTGCATC AGCAGCTGTGCTCGGTAAACTCCCAGCTGGAGCCGTGTCTGCCGGTGTTCCCCGAAGAGCCGTCAGGCAT GTGGACGGTGCTGTTTGGGGGCGCCCCCGAGATGACCGAGCAGGAGATCGACGCTCTATGTTACCAACTC CAGGTATACCTGGGCCACGGCCTGGACACGTGTGGCTGGAAGATCCTTTCTCAGGTTCTTTTCACCGAGA CGGATGATCCGGAGGAGTATTACGAAAGCCTCAGCGAGCTGCGGCAGAAGGGCTATGAAGAGGTGCTTCA GCGGGCCAGGAGGCGCATCCAGGAGCTCTTGGACAAGCACAAGACTATAGAAAGCATGGTAGAGCTTTTG GACTTGTATCAGATGGAGGATGAAGCCTACAGCAGCCTTGCAGAGGCCACAACTGAACTCTACCAGTATT TACTTCAGCCATTCCGAGACATGCGAGAACTGGCCATGCTACGAAGACAGCAGATCAAGATTTCCATGGA GAATGATTATTTGGGCCCTCGAAGAATTGAGAGTCTACAGAAAGAAGATGCTGACTGGCAGCGGAAAGCT CACATGGCTGTTTTGTCTATTCAGGATCTCACCGTCAAATATTTTGAAATAACAGCAAAAGCTCAGAAAG CTGTGTATGATCGGATGCGAGCAGATCAGAAGAAATTTGGCAAAGCATCGTGGGCAGCAGCTGCTGAGCG AATGGAAAAACTCCAGTATGCAGTTTCTAAAGAGACTTTGCAGATGATGAGAGCTAAAGAAATTTGTCTG GAACAGAAGAAACATGCACTAAAAGAAGAGATGCAAAGCTTACAGGGTGGTACAGAAGCTATAGCTCGAT TGGATCAGCTGGAATCTGACTACTATGATCTGCAACTTCAGTTGTATGAAGTACAGTTTGAAATCTTGAA GTGTGAAGAGTTGTTATTAACTGCACAGCTGGAGAGCATCAAGAGACTTATATCAGAAAAGAGAGATGAA GTGGTGTACTACGACACTTACGAAAGCATGGAGGCCATGCTGGAGAAGGAAGAGATGGCAGCGTCTGTGC ACGCCCAGAGGGAAGAGCTACAGAAACTGCAGCAGAAGGCACGCCAGCTGGAAGCAAGAAGGGGCCGTGT CTCAGCCAAGAAAGCCTACCTCAGAAATAAAAAAGAAATTTGCATTGCAAAACACCATGAGAAGTTCCAG CAGCGTTTTCAGAGTGAAGATGAATATAGAGCCCATCATACAATACAAATAAAGAGAGACAAATTGCATG ATGAAGAGGAAAGAAAAAGTGCCTGGGTTAGCCAAGAGAGACAGAGGACACTGGATAGACTTCGAACATT TAAGCAGAGGTACCCCGGGCAAGTCATCCTTAAGTCGACCAGATTACGAGTGGCGCATTCAAGAAGAAAA AGCACAGCAAGCCCTGTGCCCTGTGAGGAGCAGTGTCACTCTCTGCCAACAGTGCTGCAGGGGCAGGAGA AGACAGAGGTGGGAGGAGGAGGAAGCCAGCTTGGGCCTTCACAGACAGCAGAACCCCAGAGCCTTGTCCA ACTTGAAGACACTTCATCAGAACAACTTGAATCCACCTCATTACCTCCTCGTGCTGTCGTCAGCTCTGAA CTGCCTCCTCCACAGTCAGCTCCACTGTTGACTAGTATTGACCCCAAACCGTGTTCTGTTACTATAGATC CTCTCCCACCCCCTCTTCCTCCAACACCTCCCCCTCCCCCACCCCCACCCCCACCTCCACCCCCACCCCT GCCTGTTGCAAAGGACAATGGGGCCTCCACCACTGCAGAGACACTGGAGAAAGATGCACTTAGGACGGAG GGCAATGAGAGGAGCATCCCAAAGTCGGCCAGTGCCCCCGCAGCACACCTCTTTGATAGCAGCCAGCTGG TCAGCGCACGGAAAAAGCTCAGAAAGACTGTGGAAGGGCTGCAGAGGAGGAGAGTGAGTTCACCCATGGA TGAAGTGTTAGCATCCTTGAAGCGTGGTAGCTTTCATCTGAAAAAGGTTGAACAGCGGACTCTGCCTCCT TTTCCTGATGAAGATGATAGTAATAATATTTTGGCGCAGATAAGGAAAGGGGTAAAGTTGAAGAAGGTTC AGAAGGAAGTTTTGAGAGAATCCTTCACACTTCTGCCTGATACCGACCCTTTGACACGGAGTATCCACGA AGCTCTAAGAAGAATCAAAGAAGCGTCCCCAGAGTCAGAGGATGAGGAAGAGGCTTTGCCGTGCACAGAC TGGGAGAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211383 representing NM_021310
Red=Cloning site Green=Tags(s) MSFALEETLESDWVAVRPHVFDEREKHKFVFIVAWNEIEGKFAITCHNRTAQRQRSGSREQAGTPASDGS RGPGSPAARGRSEAAASATAALRSPGPRKSQAWAEGGSPRSARSLKGDPPRGPAGRGPESPLRSPARAKA SPLRRSAESRDATASATPAPPAPPVPPVSSVRVVSASGAVSEEIEVLEMVREDEAPQPLPDSEQPPSAAE LESPAEECSWAGLFSFQDLRAVHQQLCSVNSQLEPCLPVFPEEPSGMWTVLFGGAPEMTEQEIDALCYQL QVYLGHGLDTCGWKILSQVLFTETDDPEEYYESLSELRQKGYEEVLQRARRRIQELLDKHKTIESMVELL DLYQMEDEAYSSLAEATTELYQYLLQPFRDMRELAMLRRQQIKISMENDYLGPRRIESLQKEDADWQRKA HMAVLSIQDLTVKYFEITAKAQKAVYDRMRADQKKFGKASWAAAAERMEKLQYAVSKETLQMMRAKEICL EQKKHALKEEMQSLQGGTEAIARLDQLESDYYDLQLQLYEVQFEILKCEELLLTAQLESIKRLISEKRDE VVYYDTYESMEAMLEKEEMAASVHAQREELQKLQQKARQLEARRGRVSAKKAYLRNKKEICIAKHHEKFQ QRFQSEDEYRAHHTIQIKRDKLHDEEERKSAWVSQERQRTLDRLRTFKQRYPGQVILKSTRLRVAHSRRK STASPVPCEEQCHSLPTVLQGQEKTEVGGGGSQLGPSQTAEPQSLVQLEDTSSEQLESTSLPPRAVVSSE LPPPQSAPLLTSIDPKPCSVTIDPLPPPLPPTPPPPPPPPPPPPPPLPVAKDNGASTTAETLEKDALRTE GNERSIPKSASAPAAHLFDSSQLVSARKKLRKTVEGLQRRRVSSPMDEVLASLKRGSFHLKKVEQRTLPP FPDEDDSNNILAQIRKGVKLKKVQKEVLRESFTLLPDTDPLTRSIHEALRRIKEASPESEDEEEALPCTD WEN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_021310 |
| ORF Size | 2949 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_021310.3, NP_067285.2 |
| RefSeq Size | 8780 bp |
| RefSeq ORF | 2952 bp |
| Locus ID | 57748 |
| UniProt ID | Q9QXM1 |
| MW | 110.6 kDa |
| Gene Summary | Acts both as a nuclear p53/TP53-cofactor and a cytoplasmic regulator of actin dynamics depending on conditions. In nucleus, acts as a cofactor that increases p53/TP53 response via its interaction with p300/EP300. Increases p53/TP53-dependent transcription and apoptosis, suggesting an important role in p53/TP53 stress response such as DNA damage. In cytoplasm, acts as a nucleation-promoting factor for both branched and unbranched actin filaments. Activates the Arp2/3 complex to induce branched actin filament networks. Also catalyzes actin polymerization in the absence of Arp2/3, creating unbranched filaments. Contributes to cell motility by controlling actin dynamics. May promote the rapid formation of a branched actin network by first nucleating new mother filaments and then activating Arp2/3 to branch off these filaments. The p53/TP53-cofactor and actin activator activities are regulated via its subcellular location.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222946 | Jmy (untagged) - Mouse junction-mediating and regulatory protein (Jmy), (10ug) |
CNY 12,640.00 |
|
| MG211383 | Jmy (tGFP-tagged) - Mouse junction-mediating and regulatory protein (Jmy) |
CNY 7,890.00 |
|
| MR211383L3 | Lenti ORF clone of Jmy (Myc-DDK-tagged) - Mouse junction-mediating and regulatory protein (Jmy) |
CNY 9,030.00 |
|
| MR211383L4 | Lenti ORF clone of Jmy (mGFP-tagged) - Mouse junction-mediating and regulatory protein (Jmy) |
CNY 9,030.00 |
