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Ankrd27 (BC065093) Mouse Tagged ORF Clone
CAT＃: MR211401
- TrueORF®
Ankrd27 (Myc-DDK-tagged) - Mouse ankyrin repeat domain 27 (VPS9 domain) (cDNA clone MGC:85985 IMAGE:6855501)
ORF Plasmid: tGFP
"BC065093" in other vectors (4)
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CNY 7,272.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MGC25907, Varp |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211401 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCTATATGATGAAGATCTCCTGAAAAACCCTTTCTACCTGGCCCTTCAGAAGTGGCGCCCCGACT TGTGCAGCAAGGTGGCCCAGATCCATGGCATTGTCCTAGTGCCTTGCAGAGGAAGCCTGCCCGGCAGTGT GCAGGCCTCCTGCCAGTTCGAGTCCTATGTTTTGGTACCCACGGAAGGACACTTTCAGACCTTAGATGGA AAGGCTGTCGTCATTGAAGGAAACAGGATTAAGCTAGGAGCAGGTTTTGCTTGCCTTCTCTCTGTGCCCA TCCTCTTTGAGGAGACTTTCTACAACGAGAAAGAGGAGAGTTTCAGCATTCTCTGCATTGCTCATCCTTT GGAGAGGAGAGAGACTTCAGAAGAACCTTCAGCGCCTGCAGATCCTTTCTCCCTGAAAACCATCGAAGAT GTGAGAGAATTTTTGGGAAGACACTCAGAGAAATTTGACAAGAACATTGCCTCTTTCCACCGGACGTTCC GAGAATGTGAAAGAAAGAGCCTCCGCCACCACATAGACTCCGTAAATGCTCTCTACACCAAATGCCTCCA GCAGCTTCTCCGAGACTCTCACCTGAAGGTTCTTGCAAAGCAGGAGGCCCAGATGAACCTGATGAAGCAG GCCGTAGAGATGTATGTCCATCACGATATTTACGACCTGATCTTTAAATACGTGGGGACCATGGAGGCGA GCGAGGATGCCGCCTTTAACAAAATCACGAGAAGCCTTCAAGATCTCCAGCAGAAAGACATCGGCGTGAA ACCCGAGTTCAGCTTCAACATCCCTCGGGCAAAGAGAGAGCTGGGTCAGTTGAACAAGTGTACGTCGCCA CAGCAGAAGCTGCTGTGCCTGAGGAAGGTGGTCCAGCTCATGACACAATCTCCCAGCCAGAGAGTGAACT TGGAGACCATGTGTGCCGATGATCTCCTCTCGGTCCTGTTATATCTGCTCGTGAAGACAGAGATCCCTAA CTGGATGGCAAATTTGAGCTACATCAAAAACTTCAGATTTAGCAGCTCGGCCAAAGATGAGCTAGGATAC TGCCTGACCTCAGTCGAGGCTGCGATTGAATACATCCGGCAAGGAAGTCTCTCCACGAAGACGCCTGACG CTGAGGGATTTGGCGACAGACTATTTCTCAAGCAGCGGATGAACTTGCTGTCCCAGATGACATCAACACC CATCGACTGCCTGTTCAAGCACATCGCCTCTGGTAACCAAAAGGAAGTGGAAAGACTTCTGAGCCAAGAT GACCAGGACAAAGACGCCATGCAAAAGATGTGCCACCCACTTTGTTCCTGTGAGGACTGTGAGAAGCTTA TCTCTGGGAGGCTGAATGACCCGTCAGTTGTCACTCCTTTCTCCAGAGATGACAGAGGTCAAACACCCCT CCATGTGGCTGCCCTCTGTGGTCAGGCATCCCTCATTGACTTCCTGGTCTCCAAAGGTGCCGTAGTGAAC GCCACAGACTATCATGGGTCCACCCCCTTACATCTGGCATGTCAGAAAGGCTTCCAAAGTGTGACGCTGC TGCTGCTGCACTACAAGGCCAGCACTGAGGTTCAGGACAACAACGGCAACACCCCGCTGCACCTGGCCTG CACCTATGGGCAGGAGGATTGCGTGAAGGCCCTGGTCTACTATGATGTCCAGGCCTGCAGATTGGATATT GGTAATGAGAAAGGAGACACAGCCCTGCACATTGCTGCACGCTGGGGTTACGAGGGCATCATAGAGACAC TGCTGCAGAATGGAGCCCCCACAGCAGTCCAGAACAGACTGAAGGAAACACCACTCAAGTGTGCTCTGAA CTCGAAGATTCTGTCTATCATGGAAGCTCATCACCTATCCTCTGACAGGAGGCCAAGGCCTTCTGAGGTT CCTGCACAGTCCCCTACACGCTCTGTGGATTCCATCAGCCAGGGCTCCTCCACCTCCAGCTTTTCTTCCA TATCGGTGAGCTTCAGGCAAGAAGAGGTCAAAAAGGACTACAGGGAGAAACTGGCCAGGATTTCTGCCAA TGGGCTTAGTGTGAATGTGACCAACCAGGACGGCTTCTCTCCCCTGCACATGGCTGCCCTGCATGGCCGC ACAGACCTCGTCCCCCTCCTGCTGAAGCACGGTGCTTACTCAGGTGCCAGAAACACAAGCCAAGCCGTCC CGCTCCACCTGGCCTGCCAGCAGGGGCACTTTCAGGTGGCAAAATGTCTCTTAGATTCCAATGCAAAACC TAATAAAAAGGACCTCAGTGGGAACACACCCCTCATTTGTGCCTGCTCTGCCGGCCACCATGAAGTCGCT GCGCTGTTGCTACAGCACGGGGCCTCCATCAATGCTTGCAACAACAAAGGCAACACGGCCCTGCATGAGG CTGTGATGGGAAGGCACACCCTGGTAGTGGAGCTGCTTCTGTTCTACGGGGCGTCGGTTGACATCCTGAA CAAGAGGCAGTACACGGCGGCGGACTGTGCCGAGCAGGACTCAAAAATAATGGAGTTGCTGCAGGTAGTG CCAGGCTGTGTTGCGTCTCTAGACAGCGTCGAGGAGGCAGACCACGAGGGTTACGTGACTGTTGAGATCA GGAGAAAATGGAACCCCAAAATGTACAACCTGCCAGAGGAACCTCTTAGGAGACAGTTTTGCCTCATTAA CCCGGGGGGTCGGTTCCAGGAGAGGACATCCAGGGAGACTATGGGAAGGGATAGAAGTGTGCCCGATCTT GCAGGACGTTCTCTTCAGGAGCCAGAGAAGCAAAGAGTCACCGGAAAGCAGAGTGACCTGTCAGACCTGA GCAGGTATCAGACCTCTGAGGAAGGAAACAAGGGGCTCCCGGAGAGGCCCGTGTCGAGACAGGCTGCTCC AGGACACCGGCCGATGGTCCGCAGACACACAGTCAATGACGCAGCCATACTCCAGGTCCCAGAGGTGACT GTCCACCTCACCACCCACGAGGCGAGTGTTCCTCAGTCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211401 protein sequence
Red=Cloning site Green=Tags(s) MALYDEDLLKNPFYLALQKWRPDLCSKVAQIHGIVLVPCRGSLPGSVQASCQFESYVLVPTEGHFQTLDG KAVVIEGNRIKLGAGFACLLSVPILFEETFYNEKEESFSILCIAHPLERRETSEEPSAPADPFSLKTIED VREFLGRHSEKFDKNIASFHRTFRECERKSLRHHIDSVNALYTKCLQQLLRDSHLKVLAKQEAQMNLMKQ AVEMYVHHDIYDLIFKYVGTMEASEDAAFNKITRSLQDLQQKDIGVKPEFSFNIPRAKRELGQLNKCTSP QQKLLCLRKVVQLMTQSPSQRVNLETMCADDLLSVLLYLLVKTEIPNWMANLSYIKNFRFSSSAKDELGY CLTSVEAAIEYIRQGSLSTKTPDAEGFGDRLFLKQRMNLLSQMTSTPIDCLFKHIASGNQKEVERLLSQD DQDKDAMQKMCHPLCSCEDCEKLISGRLNDPSVVTPFSRDDRGQTPLHVAALCGQASLIDFLVSKGAVVN ATDYHGSTPLHLACQKGFQSVTLLLLHYKASTEVQDNNGNTPLHLACTYGQEDCVKALVYYDVQACRLDI GNEKGDTALHIAARWGYEGIIETLLQNGAPTAVQNRLKETPLKCALNSKILSIMEAHHLSSDRRPRPSEV PAQSPTRSVDSISQGSSTSSFSSISVSFRQEEVKKDYREKLARISANGLSVNVTNQDGFSPLHMAALHGR TDLVPLLLKHGAYSGARNTSQAVPLHLACQQGHFQVAKCLLDSNAKPNKKDLSGNTPLICACSAGHHEVA ALLLQHGASINACNNKGNTALHEAVMGRHTLVVELLLFYGASVDILNKRQYTAADCAEQDSKIMELLQVV PGCVASLDSVEEADHEGYVTVEIRRKWNPKMYNLPEEPLRRQFCLINPGGRFQERTSRETMGRDRSVPDL AGRSLQEPEKQRVTGKQSDLSDLSRYQTSEEGNKGLPERPVSRQAAPGHRPMVRRHTVNDAAILQVPEVT VHLTTHEASVPQS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC065093 |
| ORF Size | 2979 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC065093, AAH65093 |
| RefSeq Size | 4105 bp |
| RefSeq ORF | 2981 bp |
| Locus ID | 245886 |
| MW | 110.6 kDa |
| Gene Summary | May be a guanine exchange factor (GEF) for Rab21, Rab32 and Rab38 and regulate endosome dynamics (By similarity). May regulate the participation of VAMP7 in membrane fusion events; in vitro inhibits VAMP7-mediated SNARE complex formation by trapping VAMP7 in a closed, fusogenically inactive conformation (By similarity). Involved in peripheral melanosomal distribution of TYRP1 in melanocytes; the function, which probably is implicating vesicle-trafficking, includes cooperation with Rab32, Rab38 and VAMP7 (PubMed:19403694, PubMed:21187289). Involved in the regulation of neurite growth; the function seems to require its GEF activity, probably towards Rab21, and VAMP7 but not Rab32/38 (PubMed:19745841, PubMed:22171327). Proposed to be involved in Golgi sorting of VAMP7 and transport of VAMP7 vesicles to the cell surface; the function seems to implicate kinesin heavy chain isoform 5 proteins, GOLGA4, RAB21 and MACF1. Required for the colocalization of VAMP7 and Rab21, probably on TGN sites (By similarity). Involved in GLUT1 endosome-to-plasma membrane trafficking; the function is dependent of association with VPS29 (By similarity). Regulates the proper trafficking of melanogenic enzymes TYR, TYRP1 and DCT/TYRP2 to melanosomes in melanocytes (PubMed:26620560).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220935 | Ankrd27 (untagged) - Mouse ankyrin repeat domain 27 (VPS9 domain) (cDNA clone MGC:85985 IMAGE:6855501), (10ug) |
CNY 9,120.00 |
|
| MG211401 | Ankrd27 (tGFP-tagged) - Mouse ankyrin repeat domain 27 (VPS9 domain) (cDNA clone MGC:85985 IMAGE:6855501) |
CNY 6,270.00 |
|
| MR211401L3 | Lenti ORF clone of Ankrd27 (Myc-DDK-tagged) - Mouse ankyrin repeat domain 27 (VPS9 domain) (cDNA clone MGC:85985 IMAGE:6855501) |
CNY 7,600.00 |
|
| MR211401L4 | Lenti ORF clone of Ankrd27 (mGFP-tagged) - Mouse ankyrin repeat domain 27 (VPS9 domain) (cDNA clone MGC:85985 IMAGE:6855501) |
CNY 7,600.00 |
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