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Inpp5b (NM_008385) Mouse Tagged ORF Clone
CAT＃: MR211402
- TrueORF®
Inpp5b (Myc-DDK-tagged) - Mouse inositol polyphosphate-5-phosphatase B (Inpp5b), nuclear gene encoding mitochondrial protein
ORF Plasmid: tGFP
"NM_008385" in other vectors (4)
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CNY 7,280.00
CNY 8,650.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 5PTase; 75kD; AW260155; INPP5P |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211402 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACCAGTCTGTGGCAATCCAGGAGACCCTGGTTGAGGGGGAATACTGCGTCATCGCAGTCCAAGGTG TGCTGTGTAAGGGAGATAGCCGGCAGAGCCGCCTGCTCGGGCTGGTGAGGTACCGGCTGGAAAATGACGC CCAGGAGCATGCTCTCTTCCTGTATACACACCGGAGGATGGCCATCACAGGAGACGATGTATCTCTGGAC CAGATAGTGCCACTCTCAAAAGATTTTATGCTGGAAGAAGTGTCCCCAGATGGTGAACTCTACATCCTTG GCTCTGATGTGACAGTACAATTGAACACAGCTGAACTCAAGCTTGTATTCCAACTTCCCTTTGGTTCACA CACCAGAACGTTCCTCCAGGAAGTGGCCAGAGCCTGTCCAGGTTTCGACCCTGAGACGCGGGATCCCGAG TTCGAGTGGCTGTCAAGACACACATGCGCGGAGCCCGATGCCGAGTCGCCGAAACCGCGCGAGTGGAATT CGGACCCGGGTACCCGGAGTGGGTTCGCGCCCATTGGAGGAAGCAGGCATCAGTCTCGGAATGCACGGCG AGGATTGGAGGATGTCTTGCCTAGGGGCCCTGGCTACATTCTGCTTTGGGGCGGAGCCGCTGAGGAGCCG GAATTTCTATTGGCGGAGGAGATGCATGAGGGCGGGCCTGTGAGAGGACGGAGACCGCTCGCCGGGCGGC GGGATGAAGCGCTTGAGGAAGCAGACTGGGAAATGTCGGCAGGCGGCGGCTCTCGGGAGCGCGACTGTGC AGGCGTTTCTAACGTCGACAGCTCGAGGCCAAATGGGAGGGGCCCGGACCAACCCTCCGGAGCCAGGTGT CCGGAGAAACCAGAAAACTCTCTCACAAGACAGAATAAATCTAAGTCTGACATGTCTGAAAAGGTTCGCT CCGCCACAGTCACAGTATCGGACAAGGCTCACATTTTATCCGTGCAGAAGTTTGGGCTTCGAGACACAAT CGTGAGATCACACCTCGTGCAGAAGGAAGAGAATTATACCTACATCCAGAACTTCAGGTTTTTTGTGGGA ACCTACAATGTGAACGGACAGTCCCCCAAAGAATGTCTCCGGCCCTGGCTGAGCCACAGCGCCCTGGCCC CCGACGTGTACTGTGTAGGGTTCCAGGAGCTCGACCTGAGTAAGGAAGCCTTCTTCTTTCACGATACCCC AAAGGAGGAAGAGTGGTTTAAGGCCGTGTCAGAAAGCCTTCACCCAGACGCCAAGTACGCAAAGGTGAAG TTCGTCCGACTGGTTGGGATTATGTTGCTGCTGTACGTCAAACAGGAACATGCAGCCTATATCTCAGAAG TGGAAGCTGAGACCGTAGGGACAGGAATCATGGGAAGGATGGGTAACAAAGGAGGAGTGGCGATCAGGTT CCAGCTGCACAACACCAGCATCTGCGTTGTCAATTCCCATCTGGCAGCCCACACAGAGGAGTACGAGAGG AGGAACCAAGACTACAGAGACATTTGTTCTCGAATGCAGTTCCCTCAGGTGGATCCGAGCCAACCCCCTC TCACCATTAACAAGCATGATGTGATCTTGTGGCTGGGAGACCTCAACTACAGGATAGAAGAGCTGGATGT GGGAAAGGTGAAAAAGCTCGTGGAAGAGAAGGCCTTCCAAACCCTGTATGCGCACGACCAGCTAAAAATC CAGGTGGCTGCCAGGACCATCTTTGATGGCTTCACCGAGGGTGAGATTACATTCCAGCCTACTTACAAGT ACGATACTGGCTCTGACGACTGGGACACCAGTGAGAAGTGTCGTGCACCGGCCTGGTGTGACCGAATCCT GTGGAAAGGAAAGAACATCACTCAGCTGAGTTACCAGAGCCACATGGCCTTGAAGACCAGTGACCACAAG CCTGTCAGCTCAGTGTTTGACATTGGGGTGAGGGTTGTCAATGAAGAACTCTACCGGAAGACCCTGGAGG AAATTGTCCGCTCCCTGGATAAAATGGAAAACGCCAACATTCCTTCTGTGACCCTCTCCAAGCGGGAGTT CTGTTTTGAGAATGTGAAGTACATGCAGTTGCAAACCGAGTCCTTCACGATTCATAATAGCCAAGTGCCC TGTCAGTTTGAATTCATCAACAAGCCTGACGAAGAGTCCTACTGTAAGCAGTGGCTGACGGCCAGACCCA GCAAGGGCTTCCTCCTGCCAGATTCGCATGTTGAGATTGAACTTGAGCTTTTTGTAAATAAGTCTACTGC CACAAAACTCAACTCAGGCAAGGACACGATTGAAGACATTCTGGTTTTACACTTGGAGCGGGGAAAGGAC TACTTTCTGTCTGTGTCTGGGAACTACCTGCCCAGCTGCTTCGGATCTCCTATCCATACATTGTGCTACA TGAGGGAGCCCATCTTGGATCTACCACTGAAAACTGTTAGTGACCTGACGTTGATGTCAGTGCAGACTGC AGATGACAGGAGCCAGTTGGAGAACCCCATGGAAATCCCCAAAGAGCTCTGGATGATGGTGGATTACCTG TACAGAAATGCCGTCCAGCAGGAAGATCTGTTTCAGCAGCCAGGTCTGAGGCCAGAGTTTGACCATATAA GGGACTGTCTAGATACTGGGATGATCGACCAGCTCTGTGCTAACAACCATTCTGTAGCCGAAGCCTTGCT GCTTTTCCTAGAGAGCCTGCCAGAGCCTGTCATCTGTTACAGCGCCTACCATAGCTGCTTGGAGTGTTCT GGCAATTACGCAGCAAGCAAGCAGATAATTTTAACCCTCCCCAGCTTCCACAAAAATGTCTTCAACTACT TGATGGCGTTTTTGCAAGAACTGCTGAAAAATTCAGCAAACAATCATTTGGATGAGAATATTCTAGCTAG CATATTTGGCAGCTTACTGCTCCGGAACCCAGCTCGTCACCAAAAGCTTGACATGGCGGAAAAGAAGAAG GCCCAAGAGTTTATTCACCAGTTCCTCTGCGGTCCACTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211402 protein sequence
Red=Cloning site Green=Tags(s) MDQSVAIQETLVEGEYCVIAVQGVLCKGDSRQSRLLGLVRYRLENDAQEHALFLYTHRRMAITGDDVSLD QIVPLSKDFMLEEVSPDGELYILGSDVTVQLNTAELKLVFQLPFGSHTRTFLQEVARACPGFDPETRDPE FEWLSRHTCAEPDAESPKPREWNSDPGTRSGFAPIGGSRHQSRNARRGLEDVLPRGPGYILLWGGAAEEP EFLLAEEMHEGGPVRGRRPLAGRRDEALEEADWEMSAGGGSRERDCAGVSNVDSSRPNGRGPDQPSGARC PEKPENSLTRQNKSKSDMSEKVRSATVTVSDKAHILSVQKFGLRDTIVRSHLVQKEENYTYIQNFRFFVG TYNVNGQSPKECLRPWLSHSALAPDVYCVGFQELDLSKEAFFFHDTPKEEEWFKAVSESLHPDAKYAKVK FVRLVGIMLLLYVKQEHAAYISEVEAETVGTGIMGRMGNKGGVAIRFQLHNTSICVVNSHLAAHTEEYER RNQDYRDICSRMQFPQVDPSQPPLTINKHDVILWLGDLNYRIEELDVGKVKKLVEEKAFQTLYAHDQLKI QVAARTIFDGFTEGEITFQPTYKYDTGSDDWDTSEKCRAPAWCDRILWKGKNITQLSYQSHMALKTSDHK PVSSVFDIGVRVVNEELYRKTLEEIVRSLDKMENANIPSVTLSKREFCFENVKYMQLQTESFTIHNSQVP CQFEFINKPDEESYCKQWLTARPSKGFLLPDSHVEIELELFVNKSTATKLNSGKDTIEDILVLHLERGKD YFLSVSGNYLPSCFGSPIHTLCYMREPILDLPLKTVSDLTLMSVQTADDRSQLENPMEIPKELWMMVDYL YRNAVQQEDLFQQPGLRPEFDHIRDCLDTGMIDQLCANNHSVAEALLLFLESLPEPVICYSAYHSCLECS GNYAASKQIILTLPSFHKNVFNYLMAFLQELLKNSANNHLDENILASIFGSLLLRNPARHQKLDMAEKKK AQEFIHQFLCGPL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008385 |
| ORF Size | 2982 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008385.3, NP_032411.3 |
| RefSeq Size | 3826 bp |
| RefSeq ORF | 2982 bp |
| Locus ID | 16330 |
| UniProt ID | Q8K337 |
| MW | 112.8 kDa |
| Gene Summary | This gene encodes a member of the inositol polyphosphate-5-phosphatase (INPP5) family. This protein hydrolyzes the 5' phosphate from phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol-1,4,5-trisphosphate, which results in changes to multiple signaling pathways. This protein may be involved in protein trafficking and secretion. Homozygous knockout mice exhibit impaired spermatogenesis and male sterility. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Nov 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC202892 | Inpp5b (untagged) - Mouse inositol polyphosphate-5-phosphatase B (Inpp5b), nuclear gene encoding mitochondrial protein, (10ug) |
CNY 7,280.00 |
|
| MG211402 | Inpp5b (tGFP-tagged) - Mouse inositol polyphosphate-5-phosphatase B (Inpp5b) |
CNY 9,500.00 |
|
| MR211402L3 | Lenti ORF clone of Inpp5b (Myc-DDK-tagged) - Mouse inositol polyphosphate-5-phosphatase B (Inpp5b), nuclear gene encoding mitochondrial protein |
CNY 10,550.00 |
|
| MR211402L4 | Lenti ORF clone of Inpp5b (mGFP-tagged) - Mouse inositol polyphosphate-5-phosphatase B (Inpp5b), nuclear gene encoding mitochondrial protein |
CNY 10,550.00 |
