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Ephb3 (NM_010143) Mouse Tagged ORF Clone
CAT＃: MR211403
- TrueORF®
Ephb3 (Myc-DDK-tagged) - Mouse Eph receptor B3 (Ephb3)
ORF Plasmid: tGFP
"NM_010143" in other vectors (4)
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CNY 7,272.00
CNY 8,650.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AW456895; Cek10; Etk2; HEK2; MDK5; Sek4; Tyro6 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211403 representing NM_010143
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGGAGCCCGCCCGCCGCCGGGGCTTCTGCCGCTGCTCGCTCCGCTGCTGCTGCCGCTGCTGCTTC CCGCCGGCTGCTGGGCGCTGGAAGAGACTCTCATGGACACGAAATGGGTGACGTCTGAGCTGGCATGGAC ATCTCATCCAGAGAGTGGGTGGGAAGAAGTAAGCGGCTATGATGAAGCCATGAATCCTATCCGCACGTAT CAGGTGTGTAATGTGCGTGAGTCGAGCCAGAACAACTGGCTGCGGACGGGTTTCATCTGGCGGCGGGAAG TCCAGCGCGTCTACGTGGAGCTGAAGTTCACCGTAAGAGACTGTAACAGCATTCCCAACATCCCTGGCTC CTGCAAGGAAACCTTCAACCTTTTCTACTACGAGGCTGATAGCGATGTGGCCTCAGCCTCCTCTCCCTTC TGGATGGAGAACCCCTACGTGAAAGTGGACACCATTGCGCCAGATGAGAGCTTCTCGCGGCTCGATGCCG GGCGTGTCAACACCAAGGTGCGCAGCTTCGGGCCGCTTTCCAAGGCTGGCTTCTACTTGGCCTTCCAGGA CCAAGGTGCCTGCATGTCACTCATCTCTGTGCGTGCCTTCTACAAGAAGTGTGCATCCACCACTGCAGGC TTCGCACTCTTCCCCGAGACCCTCACGGGGGCTGAGCCCACTTCGCTGGTCATTGCCCCCGGCACCTGCA TCGCTAACGCTGTGGAGGTCTCTGTACCACTCAAGCTCTACTGCAATGGCGACGGGGAGTGGATGGTGCC CGTCGGTGCCTGCACCTGCGCTACTGGCCATGAGCCAGCCGCCAAGGAGTCCCAGTGCCGTGCCTGTCCT CCTGGGAGTTACAAAGCAAAGCAAGGAGAAGGGCCCTGCCTCCCCTGTCCCCCCAATAGCCGCACCACCT CGCCGGCTGCCAGCATCTGCACCTGCCATAATAACTTCTACCGTGCAGACTCAGACTCGGCCGACAGCGC CTGCACCACGGTGCCATCTCCACCCCGGGGTGTGATCTCCAATGTGAATGAGACCTCGCTAATCCTCGAA TGGAGTGAGCCCCGGGACCTTGGCGGACGAGATGACCTCCTTTATAATGTTATCTGCAAGAAGTGCCGTG GGAGCTCTGGGGCTGGGGGTCCGGCAACCTGTTCACGCTGCGATGACAACGTAGAGTTTGTACCTCGGCA GCTGGGCCTGACAGAGCGCCGGGTCCACATTAGCCACCTGTTGGCCCACACACGCTACACCTTTGAGGTG CAGGCTGTCAACGGTGTCTCAGGCAAAAGCCCTTTGCCACCCCGCTATGCAGCTGTGAATATCACCACCA ACCAGGCTGCTCCATCAGAAGTGCCTACACTCCACCTGCACAGCAGCTCGGGGAGCAGCCTGACCCTGTC CTGGGCACCCCCAGAGCGGCCTAATGGAGTCATCTTGGACTATGAGATGAAGTACTTTGAGAAGAGTAAA GGCATCGCCTCCACTGTAACCAGCCAGAAGAACTCCGTACAACTGGACGGGCTGCAGCCTGATGCCCGCT ACGTAGTTCAGGTCCGGGCTCGCACAGTAGCGGGTTACGGACAGTATAGCCACCCGGCTGAGTTTGAGAC CACAAGTGAAAGAGGTTCAGGTGCCCAGCAGCTTCAAGAACAGCTTCCCCTGATTGTGGGATCCACCGTA GCCGGGTTTGTCTTCATGGTGGTCGTCGTGGTCATCGCTCTTGTCTGCCTCAGGAAGCAGCGCCATGGCC CCGATGCAGAATACACAGAGAAGCTGCAGCAATACATTGCTCCTGGGATGAAAGTTTACATTGACCCCTT TACCTATGAGGATCCCAATGAGGCCGTCCGAGAGTTCGCCAAGGAGATCGATGTGTCCTGTGTCAAGATC GAGGAGGTGATTGGAGCTGGGGAGTTTGGGGAAGTGTGCCGGGGTCGACTGAAACTGCCCGGCCGCCGGG AGGTGTTCGTGGCTATCAAGACGCTGAAGGTGGGATACACGGAGAGGCAGCGGCGGGACTTCCTAAGTGA GGCTTCCATTATGGGTCAGTTTGACCATCCCAATATAATCCGTCTAGAGGGTGTGGTCACCAAAAGTCGT CCAGTCATGATCCTCACTGAGTTCATGGAGAACTGTGCCCTGGACTCCTTTCTACGGCTCAATGACGGAC AGTTCACGGTCATCCAGCTTGTGGGCATGTTACGAGGCATTGCTGCTGGCATGAAGTACTTGTCTGAGAT GAACTACGTGCACCGTGACCTTGCTGCCCGAAACATCCTTGTCAACAGCAACTTGGTCTGCAAAGTCTCT GACTTTGGCCTCTCCCGCTTCCTGGAGGATGACCCATCAGACCCCACCTACACCAGCTCCCTGGGTGGGA AGATCCCTATCCGCTGGACCGCCCCGGAGGCCATAGCCTATCGGAAGTTTACGTCTGCCAGCGATGTCTG GAGCTATGGGATTGTCATGTGGGAGGTCATGAGCTACGGAGAGCGACCCTACTGGGATATGAGCAACCAG GATGTCATTAATGCTGTAGAGCAAGACTATCGGTTACCACCCCCCATGGACTGCCCCACCGCGCTGCACC AGCTCATGCTGGACTGTTGGGTGCGGGACCGGAACCTCAGGCCCAAGTTCTCTCAAATCGTCAACACACT AGACAAACTTATCCGCAATGCTGCCAGCCTCAAGGTCACCGCCAGTGCCCCATCTGGCATGTCCCAGCCC CTCCTGGACCGCACAGTCCCAGATTATACGACCTTCACGACAGTGGGTGACTGGCTAGATGCCATCAAGA TGGGGAGGTATAAAGAGAGCTTTGTCGGTGCTGGGTTTGCATCCTTTGACCTGGTGGCCCAGATGACTGC AGAAGATCTGCTAAGGATTGGGGTCACTTTGGCTGGGCACCAGAAGAAGATCCTCTGCAGTATCCAGGAC ATGCGGCTGCAGATGAACCAGACCCTGCCTGTGCAGGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211403 representing NM_010143
Red=Cloning site Green=Tags(s) MAGARPPPGLLPLLAPLLLPLLLPAGCWALEETLMDTKWVTSELAWTSHPESGWEEVSGYDEAMNPIRTY QVCNVRESSQNNWLRTGFIWRREVQRVYVELKFTVRDCNSIPNIPGSCKETFNLFYYEADSDVASASSPF WMENPYVKVDTIAPDESFSRLDAGRVNTKVRSFGPLSKAGFYLAFQDQGACMSLISVRAFYKKCASTTAG FALFPETLTGAEPTSLVIAPGTCIANAVEVSVPLKLYCNGDGEWMVPVGACTCATGHEPAAKESQCRACP PGSYKAKQGEGPCLPCPPNSRTTSPAASICTCHNNFYRADSDSADSACTTVPSPPRGVISNVNETSLILE WSEPRDLGGRDDLLYNVICKKCRGSSGAGGPATCSRCDDNVEFVPRQLGLTERRVHISHLLAHTRYTFEV QAVNGVSGKSPLPPRYAAVNITTNQAAPSEVPTLHLHSSSGSSLTLSWAPPERPNGVILDYEMKYFEKSK GIASTVTSQKNSVQLDGLQPDARYVVQVRARTVAGYGQYSHPAEFETTSERGSGAQQLQEQLPLIVGSTV AGFVFMVVVVVIALVCLRKQRHGPDAEYTEKLQQYIAPGMKVYIDPFTYEDPNEAVREFAKEIDVSCVKI EEVIGAGEFGEVCRGRLKLPGRREVFVAIKTLKVGYTERQRRDFLSEASIMGQFDHPNIIRLEGVVTKSR PVMILTEFMENCALDSFLRLNDGQFTVIQLVGMLRGIAAGMKYLSEMNYVHRDLAARNILVNSNLVCKVS DFGLSRFLEDDPSDPTYTSSLGGKIPIRWTAPEAIAYRKFTSASDVWSYGIVMWEVMSYGERPYWDMSNQ DVINAVEQDYRLPPPMDCPTALHQLMLDCWVRDRNLRPKFSQIVNTLDKLIRNAASLKVTASAPSGMSQP LLDRTVPDYTTFTTVGDWLDAIKMGRYKESFVGAGFASFDLVAQMTAEDLLRIGVTLAGHQKKILCSIQD MRLQMNQTLPVQV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010143 |
| ORF Size | 2979 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_010143.1, NP_034273.1 |
| RefSeq Size | 4164 bp |
| RefSeq ORF | 2982 bp |
| Locus ID | 13845 |
| UniProt ID | P54754 |
| MW | 110.1 kDa |
| Gene Summary | Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Generally has an overlapping and redundant function with EPHB2. Like EPHB2, functions in axon guidance during development regulating for instance the neurons forming the corpus callosum and the anterior commissure, 2 major interhemispheric connections between the temporal lobes of the cerebral cortex. In addition to its role in axon guidance plays also an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and the formation of excitatory synapses. Controls other aspects of development through regulation of cell migration and positioning. This includes angiogenesis, palate development and thymic epithelium development for instance. Forward and reverse signaling through the EFNB2/EPHB3 complex also regulate migration and adhesion of cells that tubularize the urethra and septate the cloaca. Finally, plays an important role in intestinal epithelium differentiation segregating progenitor from differentiated cells in the crypt.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC202000 | Ephb3 (untagged) - Mouse Eph receptor B3 (Ephb3), (10ug) |
CNY 7,280.00 |
|
| MG211403 | Ephb3 (tGFP-tagged) - Mouse Eph receptor B3 (Ephb3) |
CNY 9,500.00 |
|
| MR211403L3 | Lenti ORF clone of Ephb3 (Myc-DDK-tagged) - Mouse Eph receptor B3 (Ephb3) |
CNY 10,550.00 |
|
| MR211403L4 | Lenti ORF clone of Ephb3 (mGFP-tagged) - Mouse Eph receptor B3 (Ephb3) |
CNY 10,550.00 |
