Wdr62 (BC057041) Mouse Tagged ORF Clone

CAT#: MR211422

  • TrueORF®

Wdr62 (Myc-DDK-tagged) - Mouse WD repeat domain 62 (cDNA clone MGC:67271 IMAGE:6817055)

ORF Plasmid: DDK tGFP



  "BC057041" in other vectors (4)

Need custom modification / cloning service?
Get a free quote

Mouse Protein / Lysate Request

CNY 6,968.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms MGC31423
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR211422 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGGTCAGTCCTGACGGCCAGCACTTGGCTTCAGGCGACCGCAGTGGAAATCTGAGGATCCACGAGC
TGCACTTCATGGATGAGCTGATCAAGGTGGAGGCCCACGATGCTGAGGTGCTGTGCCTGGAGTACTCCAA
GCCTGAGACAGGAGTGACTTTGCTGGCTTCAGCCAGTCGGGACAGACTCATCCATGTGTTAAACGTGGAG
AAGAACTACAACCTGGAGCAGACCCTGGACGACCACTCCTCCTCCATCACAGCCATTAAGTTTGCTGGCA
CCAGAGATGTCCAGATGATCAGTTGTGGAGCTGACAAGAGCATCTACTTTCGCAGTGCCCAGCAGGCCTC
GGATGGACTGCACTTTGTCCGTACCCACCACGTAGCAGAGAAGACCACCTTGTATGACATGGATATCGAC
ATCACACAGAAGTATGTGGCAGTGGCCTTCCAGGACCGCAACGTAAGGGTCTACAACACCGTGAGTGGGA
AACAGAAGAAGTGCTACAAGGGTTCCCAGGGCGACGAAGGGTCCCTGCTGAAGGTCCACGTGGACCCCTC
AGGCACCTTCCTGGCCACAAGCTGCTCTGACAAAAGCATCTCCTTGATTGACTTTTACTCGGGCGAGTGT
GTTGCCAAGATGTTTGGCCATTCGGAAATTGTCACTGGCATGAAGTTCACCTACGACTGCCGCCACTTGA
TCACAGTATCTGGAGACAGCTGTGTGTTCATCTGGCACCTGGGCCCGGAGATCACCACCTGCATGAAGCA
GCACTTGCTGGAGATCAACCACCAGGAGCAGCAGCAGCAGCCCAAGGACCAGAAGTGGAGTGGCCCTCCC
AGCCAGGAGACATATGCATCCACACCAAGTGAGATTCGTTCCCTGAGCCCTGGAGAGCAGACAGAGGATG
AGATGGAGGAGGAATGTGAGCCAGAAGAGTTGCTGAAAACACCATCCAAAGACAGCTTGGACCCAGATCC
TCGATGCCTGCTGACAAATGGCAAGCTGCCACTCTGGGCAAAGCGGCTGCTAGGGGATGATGATGTGGCA
GACAGCTCAGCTTTCCACGCCAAGCGCAGCTACCAGCCACATGGCCGTTGGGCAGAGCGGGCTGAGCAGG
AACCCCTCAAGACCATCCTGGATGCCTGGAGCCTGGATTCCTACTTTACACCCATGAAGCCTGAAAATCT
CCAAGACTCTGTTCTGGACTCAGTAGAGCCACAGAACCTGGCAGGCCTGCTAAGTGAGTTAGGGAGTCCC
AAAGAGGACAACCGAGGCCACCCCTCCTACCTGCCTCTACAGAGGGAAGCCACCGAGGCAAGCGAACTCA
TCCTCTGCTCCCCAGAGGCGGAAGTGTCACTTACAGGGATGCACAGGGAGTATTATGAGGAGGAGACAGA
GGCAGGACCTGAAGACCAGCAAGGCGACACCTATCTCAGGGTCTCTTCCGTCAGTTCAAAGGATCAGAGC
CCCCCTGAGGACTCAGGGGAGTCAGAGGCTGAACTGGAGTGCAGCTTTGCCGCCGCCCACAGCTCAGCTC
CTCAAACGGACCCTGGCCCTCACCTCACCATGACAGCAGAGTACCCAAGTACAGAAGAGCTTTCCCAGCC
TGAGCTGCCAGGCTTGGGCAATGGCTCCTTACCCCAGACACCTGAGCAGGAGAAGTTCCTCCGCCACCAT
TTTGAGACACTTACTGATGCCCCTACTGAAGAGCTCTTTCATGGATCCCTGGGAGACATAAAGATCTCAG
AGACTGAGGACTATTTCTTCAATCCCCGGCTGAGCATATCCACCCAGTTCCTCTCCCGCCTCCAGAAGAC
CTCCAGGTGCCCTCCCCGACTGCCCCTGCACCTTATGAAGTCTCCAGAGGCTCAGCCTGTGGGCCAAGGG
GGCAACCAGCCCAAAGCAGGGCCCCTGAGAGCAGGTACTGGCTACATGTCCTCAGATGGGACCAACGTCC
TCTCTGGGCAGAAGGCTGAAGAAACTCAAGAGGCCTTGAGTCTACTGGACAGGAAGCCTCCAACACCCAC
ATCTGTACTGACCACAGGCCGGGAGCAAAGTATCTCCGCCCCATCTTCGTGTTCTTACCTGGAGTCCACA
ACGAGCTCACATGCCAAGACGACACGCAGCATCTCTCTTGGGGACAGTGAGGGCCCTGTGACAGCTGAGC
TACCCCAGTCACTTCACAAGCCCTTATCCCCTGGCCAGGAACTCCAAGCCATCCCCACTACAGTGGCACT
GACCTCCAGCATCAAAGACCACGAGCCTGCACCGCTTTCCTGGGGCAACCATGAGGCCCGAGCCAGCCTG
AAACTGACCTTATCCAGTGTCTGTGAGCAGCTGCTCTCTCCACCTCCACAGGAGCCACCCATCACCCATG
TGTGGTCTCAGGAACCTGTGGATGTCCCACCTAGTATGGCAGTCACAGTAGCCAGCTTCTGTGCACCCAG
CCCTGTAGACATGAGCACCCTGGGACTCCACAGTTCTATGTTTCTCCCAAAGACCTCAGCCTCTGGGCCC
CTAACCCCTCCTGCCCACCTCCAACTTCTAGAGACCAGGTCTAGGGTGCCTGGCAGCACTGCTGCTCTCC
TGGAGCCCACCCCCGATGCATCAGGTGTGATCGCAGACAGCCCTGGACACTGGGACACAGAAGTCCCTAC
CCCAGAACTCCTAGGCAGTGTGGAATCAGTCTTGCACAGGCTGCAGACTGCCTTCCAAGAAGCTCTTGAC
CTTTACCGCATGCTGGTCTCCAGCAGCCAGCTGGGTCCTGAGCAGCAGCAGGCACAGACCGAGCTGGCCT
CCACCTTCCACTGGATCCTCAACCAGCTAGAAGCCAGCAACTGTATGGCTGCAGCTAACTTGGCCCCACC
CCAGACACTGCCTAGCCCAGACCCTCTGTCCCTACCTACACTTTGTCCCCTGGCCAGCCCAAACCTGCAG
GCCCTGCTGGAACACTACTCAGAGTTGCTGGTGCAAGCAGTGAGGAGGAAGGCAAGGGGTGAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR211422 protein sequence
Red=Cloning site Green=Tags(s)

MQVSPDGQHLASGDRSGNLRIHELHFMDELIKVEAHDAEVLCLEYSKPETGVTLLASASRDRLIHVLNVE
KNYNLEQTLDDHSSSITAIKFAGTRDVQMISCGADKSIYFRSAQQASDGLHFVRTHHVAEKTTLYDMDID
ITQKYVAVAFQDRNVRVYNTVSGKQKKCYKGSQGDEGSLLKVHVDPSGTFLATSCSDKSISLIDFYSGEC
VAKMFGHSEIVTGMKFTYDCRHLITVSGDSCVFIWHLGPEITTCMKQHLLEINHQEQQQQPKDQKWSGPP
SQETYASTPSEIRSLSPGEQTEDEMEEECEPEELLKTPSKDSLDPDPRCLLTNGKLPLWAKRLLGDDDVA
DSSAFHAKRSYQPHGRWAERAEQEPLKTILDAWSLDSYFTPMKPENLQDSVLDSVEPQNLAGLLSELGSP
KEDNRGHPSYLPLQREATEASELILCSPEAEVSLTGMHREYYEEETEAGPEDQQGDTYLRVSSVSSKDQS
PPEDSGESEAELECSFAAAHSSAPQTDPGPHLTMTAEYPSTEELSQPELPGLGNGSLPQTPEQEKFLRHH
FETLTDAPTEELFHGSLGDIKISETEDYFFNPRLSISTQFLSRLQKTSRCPPRLPLHLMKSPEAQPVGQG
GNQPKAGPLRAGTGYMSSDGTNVLSGQKAEETQEALSLLDRKPPTPTSVLTTGREQSISAPSSCSYLEST
TSSHAKTTRSISLGDSEGPVTAELPQSLHKPLSPGQELQAIPTTVALTSSIKDHEPAPLSWGNHEARASL
KLTLSSVCEQLLSPPPQEPPITHVWSQEPVDVPPSMAVTVASFCAPSPVDMSTLGLHSSMFLPKTSASGP
LTPPAHLQLLETRSRVPGSTAALLEPTPDASGVIADSPGHWDTEVPTPELLGSVESVLHRLQTAFQEALD
LYRMLVSSSQLGPEQQQAQTELASTFHWILNQLEASNCMAAANLAPPQTLPSPDPLSLPTLCPLASPNLQ
ALLEHYSELLVQAVRRKARGD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC057041
ORF Size 3003 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC057041, AAH57041
RefSeq Size 3265 bp
RefSeq ORF 3005 bp
Locus ID 233064
MW 109.5 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...