Tcof1 (BC021514) Mouse Tagged ORF Clone

CAT#: MR211447

  • TrueORF®

Tcof1 (Myc-DDK-tagged) - Mouse Treacher Collins Franceschetti syndrome 1, homolog (cDNA clone MGC:36035 IMAGE:5351783)

ORF Plasmid: DDK tGFP



  "BC021514" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 10,070.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms AA408847; AW209012; treacle
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR211447 representing BC021514
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGAGGCCAGGAAGCGGCGGGAGCTGCTTCCCCTCATTTACCATCATCTGTTGCAAGCAGGCTACG
TCCGCGCGGCGCGGGAAGTAAAGGAGCAGAGCGGCCAGAAGAGTTTCCTGACTCAGCCCGTCACCCTTCT
GGACATCTATACACACTGGCAACAGACCTCAGAGCTTGGCCAGAAGCAGAAGGCAGAGGATGATGAGACC
CTTCAGGCTAAGAAGTCTCGAGTGTCGGATCCTGTTAGCAGCTCAGAGAGCTCGGATCAGGAGAAGGAAG
AGGAGGCAGCAACCGAAAGGGCCAAAGCCACCCCAAGACCGACACCTGTCAATTCTGCAACCGCAGCTTT
GCCATCAAAAGTAAAAGAAAAGGGAAAGACCAAGACAGCCAACAAGACGGTGAACTCTGTGTCGCACCCT
GGGTCCGGAAAGACGGTGGTCCACCTGCTCTCTGGGAAGTCACCCAAGAAGTCAGCAGAGCCCTTGGCAA
ACACTGTCTTGGCCTCAGAAACTGAGGAGGAGGGCAATGCCCAAGCCCTCGGACCCACTGCCAAGTCTGG
AACGGTGTCAGCGGGCCAAGGCAGCAGTTCCAGTGAAGATTCCTCCATCTCAAGCGATGAGACAGATGTC
GAGGTGAAATCTCCAGCAAAACCAGCCCAGGCCAAAGCTTCAGCAGCCCCTGCCAAGGATCCTCCAGCAA
GAACAGCCCCAGGCCCTACCAAGTTAGGGAATGTGGCGCCCACACCTGCTAAACCAGCCAGGGCGGCAGC
GGCAGCTGCTGCTGCTGCTGTGGCTGCTGCTGCTGCTGCAGCAGCAGAAGAGTCTGAGAGCAGTGAGGAG
GACTCAGACAGTGAGGACGAGGCCCCTGCTGGTCTGCCCAGCCAGGTAAAAGCCTCTGGAAAAGGTCCCC
ATGTCAGAGCCGACTCGGTGTCTGCCAAGGGGATCTCTGGGAAAGGGCCCATCTTAGCAACCCCAGGGAA
GACTGGGCCTGCAGCCACCCAGGCCAAGGCAGAAAGGCCAGAGAAGGACTCGGAAACCAGCAGTGAGGAC
GATTCTGATAGTGAGGATGAAATGCCAGTCACTGTGAATACTCCTCAGGCAAGGACTTCTGGGAAGAGCC
CTCGGGCCAGAGGTACCTCAGCCCCCGCCAAGGAGTCATCCCAGAAAGGGGCTCCTGCAGTCACCCCTGG
AAAGGCAAGGCCTGTGGCAGCCCAGGCAGGGAAACCAGAAGCCAAGAGCAGTGAGGAGTCAGAGAGTGAC
AGTGGGGAGACACCAGCTGCTGCGACTCTGACCACGAGTCCTGCCAAGGTGAAACCTTTGGGGAAGAGCT
CCCAGGTCAGACCTGTTTCCACCGTCACCCCGGGGTCATCGGGAAAAGGTGCCAACCTGCCCTGCCCTGG
GAAGGTGGGGTCAGCAGCTCTCAGGGTCCAAATGGTAAAGAAAGAAGATGTCTCGGAGAGCAGCAGTGCA
GAGCTGGACAGTGACGGGCCTGGGAGCCCAGCCAAGGCAAAGGCCTCCCTTGCGCTCCCTCAGAAGGTGA
GGCCTGTGGCCACCCAGGTCAAGACTGACAGGGGCAAAGGCCACTCAGGGAGCAGTGAGGAGTCATCTGA
CAGCGAAGAGGAGGCAGCACCAGCAGCCTCTGCTGCTCAGGCTAAGCCAGCTCTGGAAAAGCAGATGAAA
GCTTCCTCTAGGAAAGGCACGCCTGCATCCGCAACAGGAGCGAGCACCTCGTCCCATTGTAAGGCAGGAG
CGGTGACCTCTTCAGCCAGCCTGTCATCCCCAGCTCTGGCCAAGGGCACCCAGAGGTCAGATGTGGACTC
TTCCAGTGAGTCTGAGTCAGAAGGAGCTGCTCCCAGCACCCCCAGGGTACAGGGGAAGTCTGGGGGCAAG
GGCCTCCAAGGGAAAGCTGCCTTGGGGCAAGGGGTGGCCCCAGTGCACACTCAGAAGACAGGGCCTTCGG
TCAAAGCTATGGCTCAGGAAGACTCAGAGAGCCTCGAGGAGGACTCCAGCAGCGAGGAAGAGGATGAGAC
CCCAGCACAGGCCACGCCCTTGGGGAGACTTCCTCAGGCCAAAGCCAACCCACCTCCCACTAAGACACCT
CCAGCGTCTGCATCTGGAAAAGCTGTGGCTGCTCCAACCAAGGGAAAACCACCTGTTCCGAACAGCACCG
TCTCTGCAAGGGGCCAGCGGTCTGTGCCAGCCGCGGGAAAAGCAGGGGCCCCAGCAACCCAAGCCCAGAA
GGGTCCCGTGGCTGGCACAGGGGAGGACTCAGAGAGCAGCAGTAAAGAGGAGTCTGACAGTGAAGAAGAG
ACGCCAGCCCAGATAAAACCTGTGGGGAAGACCTCTCAGGTCAGAGCTGCCTCAGCCCCTGCCAAGGAGT
CTCCTAAAAAAGGAGCCCATCCAGGAACCCCCGGCAAGACGGGATCTTCAGCTACCCAGGCCCAGCCAGG
GAAGACAGAGGACTCAGACAGCAGTAGTGAAGAGTCTGACAGTGACACAGAGATGCCATCAGCCCAGGAT
GCTATCTCCCAGCCTGCCAGAGGCAAAGCGTCAGGTCCAGCGTCCCCAGAGAAGAGCATAGAAGGGTCCT
CAGAGAGCAGTGATGAGGATCTGCCCTCCGGCCAGGCGATTAAATCCCCTCCAGTTTCTGTCAACCGTAA
CAGTAGTCCAGCTGTCCCAGCTCCTACCCCAGAAGGAGTCCAGGCTGTGAACACCACAAAGAAGGCCTCA
GGCACCACTGCCCAGAGCTCCTCCTCTGAGAGTGAGGACGGGGACGAGGACTTGATTCCTGCCACACAAC
CCTCCACCTATGCTCTCAGAACCAGTGTGACGACGCCCGCAGCCCTCTCACGAGCAGCTTCCCAACCCAG
CAAGAGTGAGCAGTCTAGCCGGATGCCAAAAGGCAAGAAAGCAAAGGCGGCGGCGTCCGCTCAGGTACTG
TCGGACCAGCAGTGCCGTGGAAACACTCCCCATGATGCCTCCCCAGAGCGCACCCATCCAGCCCAAAGCC
ACCAACAAGCTCGGGAAATCCAAGCTCCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR211447 representing BC021514
Red=Cloning site Green=Tags(s)

MAEARKRRELLPLIYHHLLQAGYVRAAREVKEQSGQKSFLTQPVTLLDIYTHWQQTSELGQKQKAEDDET
LQAKKSRVSDPVSSSESSDQEKEEEAATERAKATPRPTPVNSATAALPSKVKEKGKTKTANKTVNSVSHP
GSGKTVVHLLSGKSPKKSAEPLANTVLASETEEEGNAQALGPTAKSGTVSAGQGSSSSEDSSISSDETDV
EVKSPAKPAQAKASAAPAKDPPARTAPGPTKLGNVAPTPAKPARAAAAAAAAAVAAAAAAAAEESESSEE
DSDSEDEAPAGLPSQVKASGKGPHVRADSVSAKGISGKGPILATPGKTGPAATQAKAERPEKDSETSSED
DSDSEDEMPVTVNTPQARTSGKSPRARGTSAPAKESSQKGAPAVTPGKARPVAAQAGKPEAKSSEESESD
SGETPAAATLTTSPAKVKPLGKSSQVRPVSTVTPGSSGKGANLPCPGKVGSAALRVQMVKKEDVSESSSA
ELDSDGPGSPAKAKASLALPQKVRPVATQVKTDRGKGHSGSSEESSDSEEEAAPAASAAQAKPALEKQMK
ASSRKGTPASATGASTSSHCKAGAVTSSASLSSPALAKGTQRSDVDSSSESESEGAAPSTPRVQGKSGGK
GLQGKAALGQGVAPVHTQKTGPSVKAMAQEDSESLEEDSSSEEEDETPAQATPLGRLPQAKANPPPTKTP
PASASGKAVAAPTKGKPPVPNSTVSARGQRSVPAAGKAGAPATQAQKGPVAGTGEDSESSSKEESDSEEE
TPAQIKPVGKTSQVRAASAPAKESPKKGAHPGTPGKTGSSATQAQPGKTEDSDSSSEESDSDTEMPSAQD
AISQPARGKASGPASPEKSIEGSSESSDEDLPSGQAIKSPPVSVNRNSSPAVPAPTPEGVQAVNTTKKAS
GTTAQSSSSESEDGDEDLIPATQPSTYALRTSVTTPAALSRAASQPSKSEQSSRMPKGKKAKAAASAQVL
SDQQCRGNTPHDASPERTHPAQSHQQAREIQAP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC021514
ORF Size 3039 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC021514.1
RefSeq Size 4088 bp
RefSeq ORF 3041 bp
Locus ID 21453
MW 149.8 kDa
Gene Summary Nucleolar protein that acts as a regulator of RNA polymerase I by connecting RNA polymerase I with enzymes responsible for ribosomal processing and modification. Required for neural crest specification: following monoubiquitination by the BCR(KBTBD8) complex, associates with NOLC1 and acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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