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Ppfia3 (BC058404) Mouse Tagged ORF Clone
CAT＃: MR211515
- TrueORF®
Ppfia3 (Myc-DDK-tagged) - Mouse protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 3
ORF Plasmid: tGFP
"BC058404" in other vectors (3)
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CNY 10,450.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2410127E16Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211515 representing BC058404
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGTGCGAGGTGATGCCTACCATCAGCGAGGATGGCCGGCGGGGCTCGGCGCTGGGCCCGGACGAGG CCGGCGGCGAGCTGGAGCGCCTCATGGTCACGATGCTCACAGAGCGCGAGCGCCTGCTGGAGACGCTGCG AGAGGCGCAGGACGGGTTGGCGACAGCGCAGCTACGACTGCGCGAGCTCGGCCACGAGAAGGACTCGCTG CAGCGGCAGCTCAGCATCGCTCTGCCTCAGGAGTTTGCGGCTCTGACGAAGGAGCTTAACTTATGTCGGG AACAGCTTCTGGAGAGGGAAGAAGAGATCGCAGAGCTGAAGGCGGAGCGAAACAACACCCGGCTCCTTCT GGAACACCTTGAGTGCCTGGTGTCCAGGCACGAACGTTCCCTGCGAATGACTGTGGTGAAACGCCAAGCC CAGTCCCCTGGAGGGGTCTCCTCTGAGGTGGAGGTGCTCAAGGCTCTAAAATCCCTCTTTGAACACCACA AAGCCCTGGATGAGAAGGTCCGGGAAAGATTGCGGATGGCATTGGAGAATGAGTTGGCCAGCAAAGAGTC CCTGTACAGACAGAGTGAAGAGAAGAGCCGTCAGCTGGCAGAGTGGCTGGATGACGCCAAGCAGAAGCTG CAACAGACCCTGCAGAAGGCCGAGACCTTACCAGAGATAGAGGCTCAGCTGGCGCAGCGCGTGGCTGCTC TGAACAAGGCTGAGGAACGCCATGGGAACTTTGAAGAGAGGCTTCGGCAGCTGGAGGCACAGCTGGAGGA GAAGAACCAGGAGCTGCAGCGGGCCCGGCAGAGGGAGAAAATGAACGATGACCACAATAAGCGACTGTCA GAGACGGTGGACAAGCTGCTAAGTGAATCCAACGAACGGTTACAGCTTCACCTCAAAGAACGTATGGGGG CACTAGAGGAGAAGAACTCACTGAGTGAGGAGATTGCTAACATGAAGAAACTTCAAGATGAGCTGCTGCT GAATAAGGAGCAGCTCTTGGCTGAGATGGAACGGATGCAGATGGAGATCGACCAGCTCCGGGGAAGGCCG CCCTCCTCCTATTCCAGGTCCCTTCCTGGCAGTGCCCTGGAGCTCCGTTACTCCCAGGCACCAACCTTAC CTTCTGGTGCCCCTCTGGATCCTTATGGGGCTGGCAGTGGCCGGGCAGGCAAGAGGGGCCGCTGGTCGGG GGCCAAGGATGAGTCTTCCAAGGACTGGGATCGGTCTGCCCCTGCTGGGTCCATCCCACCCCCGTTCCCT GGAGAGCTGGATGGCTCAGATGAAGAGGAGGCCGAGGGGATGTTCGGGGCTGAGCTGCTGTCCCCCAGTG GGCAAGCTGATGTGCAGACGCTCGCTATCATGCTCCAGGAGCAATTGGAAGCCATCAACAAGGAAATCAA GCTAATTCAAGAAGAGAAAGAGACGACAGAGCAGCGTGCAGAAGAGCTGGAGAGCCGGGTGTCCAGTTCT GGCCTTGACTCGCTGGGCCGGTACCGAAGCAGCTGCTCACTGCCACCCTCCCTCACCACCTCCACCCTCG CCAGCCCCTCACCTCCCAGCTCTGGCCATTCCACCCCTCGTCTGGCACCCCCCAGTCCTGCCCGGGAGGG TACTGACAAGACCAACCATGTCTCTAAGGAGGAAGCTGGAGTCCCACGAGGGGAGGGTCCAGCCGTTCCA GGAGACACCCCACCACCCACGCCCCGCTCTGCCCGTCTTGAGAGGATGGCCCAGGCTTTGGCGCTGCAAG CAGGGTCACCGGAAGATGGAGCACCTCCCCGGGGAAGTGAGAGCACCCCAGATTCCCTTCACAAAGCCCC CAAGAGGAAGAGCATCAAGTCGTCCATAGGCCGGCTCTTCGGCAAGAAAGAGAAGGGCCGGATGGGACCC CCAGGCCGGGAGAGTGTTTCTCTGGCCGGGACACCCTCAGATGAGACACTGGCCACTGACCCTCTGGGGC TAGCCAAGCTGACTGGTCCAGGAGATAAGGACAGGAGGAACAAGAGGAAGCACGAACTTCTGGAAGAGGC CTGTCGCCAGGGCCTGCCTTTTGCTGCCTGGGATGGACCCACCGTGGTCTCCTGGCTGGAGCTCTGGGTG GGCATGCCGGCGTGGTACGTGGCCGCCTGCCGGGCCAATGTCAAGAGCGGCGCCATCATGGCCAACCTGT CAGACACGGAGATCCAGAGGGAGATCGGCATCAGCAATCCACTGCACAGGCTCAAGCTACGCCTCGCCAT TCAGGAGATGGTGTCGCTCACATCACCCTCGGCTCCTGCCTCCTCACGCACTCCCACAGGAAACGTGTGG ATGACACACGAGGAGATGGAGTCTCTGACGGCAGCTACAAAACCCGAGACCAAGGAGATCAGCTGGGAGC AGATCCTGGCATATGGCGACATGAACCACGAGTGGGTGGGGAACGACTGGCTGCCCAGCCTTGGGCTGCC CCAGTACCGAAGCTACTTCATGGAGTCGCTAGTTGATGCGCGGATGTTGGACCATTTGAACAAGAAGGAA CTGCGAGGCCAACTCAAGATGGTGGACAGCTTCCACAGGGTTAGTCTGCACTATGGTATTATGTGCCTGA AGCGGCTCAACTATGACCGGAAGGACCTGGAGCGGAGGAGGGAAGAAAGCCAGACCCAAATCAGAGACGT GATGGTGTGGTCCAACGAACGGGTCATGGGTTGGGTGTCTGGTCTTGGCCTGAAGGAATTTGCTACGAAT CTCACGGAGAGTGGGGTGCACGGCGCACTGTTGGCTCTGGATGAAACCTTCGACTACTCCGATCTGGCCT TGCTTCTACAGATACCTACGCAGAATGCACAAGCTCGGCAACTGCTGGAGAAAGAATTCAGTAACCTCAT CTCCTTAGGCACAGACAGACGGCTGGATGAGGACAGTGCCAAGTCCTTCAGCCGCTCCCCATCCTGGAGG AAGATGTTCCGCGAGAAGGACCTCCGGGGTGTAACCCCCGATTCAGCCGAGATGTTACCCCCCAACTTTC GCTCAGCTGCCGCAGGAGCCCTAGGCTCTCCCGGGCTTCCTCTCCGCAAACTGCAGCCTGAAGGCCAGAC TTCTGGGAGTTCGCGAGCAGATGGTGTTTCGGTCCGGACTTACTCCTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211515 representing BC058404
Red=Cloning site Green=Tags(s) MMCEVMPTISEDGRRGSALGPDEAGGELERLMVTMLTERERLLETLREAQDGLATAQLRLRELGHEKDSL QRQLSIALPQEFAALTKELNLCREQLLEREEEIAELKAERNNTRLLLEHLECLVSRHERSLRMTVVKRQA QSPGGVSSEVEVLKALKSLFEHHKALDEKVRERLRMALENELASKESLYRQSEEKSRQLAEWLDDAKQKL QQTLQKAETLPEIEAQLAQRVAALNKAEERHGNFEERLRQLEAQLEEKNQELQRARQREKMNDDHNKRLS ETVDKLLSESNERLQLHLKERMGALEEKNSLSEEIANMKKLQDELLLNKEQLLAEMERMQMEIDQLRGRP PSSYSRSLPGSALELRYSQAPTLPSGAPLDPYGAGSGRAGKRGRWSGAKDESSKDWDRSAPAGSIPPPFP GELDGSDEEEAEGMFGAELLSPSGQADVQTLAIMLQEQLEAINKEIKLIQEEKETTEQRAEELESRVSSS GLDSLGRYRSSCSLPPSLTTSTLASPSPPSSGHSTPRLAPPSPAREGTDKTNHVSKEEAGVPRGEGPAVP GDTPPPTPRSARLERMAQALALQAGSPEDGAPPRGSESTPDSLHKAPKRKSIKSSIGRLFGKKEKGRMGP PGRESVSLAGTPSDETLATDPLGLAKLTGPGDKDRRNKRKHELLEEACRQGLPFAAWDGPTVVSWLELWV GMPAWYVAACRANVKSGAIMANLSDTEIQREIGISNPLHRLKLRLAIQEMVSLTSPSAPASSRTPTGNVW MTHEEMESLTAATKPETKEISWEQILAYGDMNHEWVGNDWLPSLGLPQYRSYFMESLVDARMLDHLNKKE LRGQLKMVDSFHRVSLHYGIMCLKRLNYDRKDLERRREESQTQIRDVMVWSNERVMGWVSGLGLKEFATN LTESGVHGALLALDETFDYSDLALLLQIPTQNAQARQLLEKEFSNLISLGTDRRLDEDSAKSFSRSPSWR KMFREKDLRGVTPDSAEMLPPNFRSAAAGALGSPGLPLRKLQPEGQTSGSSRADGVSVRTYSC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC058404 |
| ORF Size | 3129 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC058404.1 |
| RefSeq Size | 4191 bp |
| RefSeq ORF | 3131 bp |
| Locus ID | 76787 |
| MW | 153.7 kDa |
| Gene Summary | May regulate the disassembly of focal adhesions. May localize receptor-like tyrosine phosphatases type 2A at specific sites on the plasma membrane, possibly regulating their interaction with the extracellular environment and their association with substrates.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG211515 | Ppfia3 (tGFP-tagged) - Mouse protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 3 |
CNY 11,500.00 |
|
| MR211515L3 | Lenti ORF clone of Ppfia3 (Myc-DDK-tagged) - Mouse protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 3 |
CNY 12,350.00 |
|
| MR211515L4 | Lenti ORF clone of Ppfia3 (mGFP-tagged) - Mouse protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 3 |
CNY 12,350.00 |
