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Gucy2c (NM_145067) Mouse Tagged ORF Clone
CAT＃: MR211529
- TrueORF®
Gucy2c (Myc-DDK-tagged) - Mouse guanylate cyclase 2c (Gucy2c), transcript variant 2
ORF Plasmid: tGFP
"NM_145067" in other vectors (4)
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CNY 7,296.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AI893437; GC-C; Gcc |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211529 representing NM_145067
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGTCACTGCTGGGCTTGGCTGTGCGGTTACTGCTCTTCCAGCCCGCGCTGATGGTGTTCTGGGCCT CTCAGGTGAGGCAGAACTGCCGCAATGGCAGCTACGAGATCAGCGTCCTGATGATGGACAACTCAGCCTA CAAAGAACCTATGCAAAACCTGAGGGAGGCTGTGGAGGAAGGACTGGACATAGTGCGAAAGCGCCTGCGT GAAGCCGACCTAAATGTGACTGTGAACGCGACTTTCATCTACTCCGACGGTCTGATTCATAAGTCAGGTG ACTGCCGGAGCAGCACCTGTGAAGGCCTTGACCTACTCAGGGAGATTACAAGAGATCATAAGATGGGCTG CGCCCTCATGGGGCCCTCGTGCACGTATTCCACCTTCCAGATGTACCTCGACACAGAGTTGAACTATCCC ATGATTTCCGCTGGAAGTTATGGATTGTCCTGTGACTATAAGGAAACCCTAACCAGGATCCTGCCTCCAG CCAGGAAGCTGATGTACTTCTTGGTCGATTTCTGGAAAGTCAACAATGCATCTTTCAAACCCTTTTCCTG GAACTCTTCGTATGTTTACAAGAATGGATCGGAACCTGAAGATTGTTTCTGGTACCTCAATGCTCTGGAG GCTGGGGTGTCCTATTTTTCTGAGGTGCTCAACTTCAAGGATGTACTGAGACGCAGCGAACAGTTCCAGG AAATCTTAACAGGCCATAACAGAAAGAGCAATGTGATTGTTATGTGTGGCACGCCAGAAAGCTTCTATGA TGTGAAAGGTGACCTCCAAGTGGCTGAAGATACTGTTGTCATCCTGGTAGATCTGTTCAGTAACCATTAC TTTGAGGAGAACACCACAGCTCCTGAGTATATGGACAATGTCCTCGTCCTGACGCTGCCGTCTGAACAGT CCACCTCAAACACCTCTGTCGCCGAGAGGTTTTCATCGGGGAGAAGTGACTTTTCTCTCGCTTACTTGGA GGGAACCTTGCTATTTGGACACATGCTGCAGACGTTTCTTGAAAATGGAGAAAATGTCACGGGTCCCAAG TTTGCTCGTGCATTCAGGAATCTCACTTTTCAAGGCTTTGCAGGACCTGTGACTCTGGATGACAGTGGGG ACATTGACAACATTATGTCCCTTCTGTATGTGTCTCTGGATACCAGGAAATACAAGGTTCTTATGAAGTA TGACACCCACAAAAACAAAACTATTCCGGTGGCTGAGAACCCCAACTTCATCTGGAAGAACCACAAGCTC CCCAATGACGTTCCTGGGCTGGGCCCTCAAATCCTGATGATTGCCGTCTTCACGCTCACGGGGATCCTGG TAGTTCTGCTGCTGATTGCCCTCCTCGTGCTGAGAAAATACAGAAGAGATCATGCACTTCGACAGAAGAA ATGGTCCCACATTCCTTCTGAAAACATCTTTCCTCTGGAGACCAACGAGACCAACCACATCAGCCTGAAG ATTGACGATGACAGGAGACGAGACACAATCCAGAGAGTGCGACAGTGCAAATACGACAAGAAGTTGCTGC AGTCTGACTACTACAACCTGACTAAGTTCTACGGCACCGTGAAGCTGGACACCAGGATCTTTGGGGTGGT TGAGTACTGCGAGAGGGGATCCCTCCGGGAAGTGTTAAACGACACAATTTCCTACCCTGACGGCACGTTC ATGGATTGGGAGTTTAAGATCTCTGTCTTAAATGACATCGCTAAGGGGATGTCCTACCTGCACTCCAGTA AGATTGAAGTCCACGGGCGTCTCAAATCCACCAACTGCGTGGTGGACAGCCGCATGGTGGTGAAGATCAC CGACTTTGGGTGCAATTCCATCCTGCCTCCAAAAAAAGACCTGTGGACGGCCCCGGAGCACCTGCGCCAG GCCACCATCTCTCAGAAAGGAGACGTGTACAGCTTCGCCATCATTGCCCAGGAGATCATCCTCCGTAAGG AGACTTTTTACACGCTGAGCTGTCGGGATCACAATGAGAAGATTTTCAGAGTGGAAAATTCATACGGGAA ACCTTTCCGCCCAGACCTCTTCCTGGAGACTGCAGATGAGAAGGAGCTGGAGGTCTATCTACTTGTCAAA AGCTGTTGGGAGGAGGATCCAGAAAAGAGGCCAGATTTCAAGAAAATCGAGAGCACACTGGCCAAGATAT TTGGCCTTTTCCATGACCAGAAAAACGAGTCTTACATGGACACCTTGATCCGACGTCTCCAGCTGTACTC TCGAAACCTGGAACATCTGGTGGAGGAAAGGACTCAGCTGTACAAGGCGGAGAGGGACAGGGCTGACCAC CTTAACTTCATGCTCCTCCCACGGCTGGTGGTAAAGTCACTGAAGGAGAAAGGCATCGTGGAGCCAGAGC TGTACGAAGAAGTCACAATCTACTTCAGTGACATTGTGGGCTTCACCACCATCTGCAAGTATAGCACGCC CATGGAGGTGGTGGACATGCTCAACGACATCTACAAGAGCTTTGACCAGATTGTGGACCACCATGACGTC TACAAGGTAGAAACCATCGGTGACGCCTACGTGGTGGCCAGCGGTCTGCCTATGAGAAACGGCAACCGAC ACGCGGTAGACATTTCCAAGATGGCCTTGGACATCCTCAGCTTCATAGGGACCTTTGAGTTGGAGCATCT CCCTGGCCTCCCCGTGTGGATCCGCATTGGAGTTCATTCTGGGCCCTGCGCTGCTGGTGTTGTGGGGATC AAGATGCCTCGCTATTGCCTGTTTGGAGACACTGTCAACACTGCCTCCAGGATGGAATCCACCGGCCTCC CCTTGAGGATTCACATGAGCAGCTCCACCATAACCATCCTGAAGAGAACGGATTGCCAGTTCCTGTATGA AGTGAGGGGAGAAACCTACTTAAAGGGAAGAGGGACAGAGACCACATACTGGCTGACTGGGATGAAGGAC CAAGAATACAACCTGCCATCCCCACCGACAGTGGAGAACCAACAGCGTCTGCAGACTGAGTTCTCAGACA TGATCGTTAGCGCCTTACAGAAAAGACAGGCCTCGGGCAAGAAGAGCCGGAGGCCCACTCGGGTGGCCAG CTACAAGAAAGGCTTTCTGGAATACATGCAGCTGAACAATTCAGACCACGATAGCACCTATTTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211529 representing NM_145067
Red=Cloning site Green=Tags(s) MTSLLGLAVRLLLFQPALMVFWASQVRQNCRNGSYEISVLMMDNSAYKEPMQNLREAVEEGLDIVRKRLR EADLNVTVNATFIYSDGLIHKSGDCRSSTCEGLDLLREITRDHKMGCALMGPSCTYSTFQMYLDTELNYP MISAGSYGLSCDYKETLTRILPPARKLMYFLVDFWKVNNASFKPFSWNSSYVYKNGSEPEDCFWYLNALE AGVSYFSEVLNFKDVLRRSEQFQEILTGHNRKSNVIVMCGTPESFYDVKGDLQVAEDTVVILVDLFSNHY FEENTTAPEYMDNVLVLTLPSEQSTSNTSVAERFSSGRSDFSLAYLEGTLLFGHMLQTFLENGENVTGPK FARAFRNLTFQGFAGPVTLDDSGDIDNIMSLLYVSLDTRKYKVLMKYDTHKNKTIPVAENPNFIWKNHKL PNDVPGLGPQILMIAVFTLTGILVVLLLIALLVLRKYRRDHALRQKKWSHIPSENIFPLETNETNHISLK IDDDRRRDTIQRVRQCKYDKKLLQSDYYNLTKFYGTVKLDTRIFGVVEYCERGSLREVLNDTISYPDGTF MDWEFKISVLNDIAKGMSYLHSSKIEVHGRLKSTNCVVDSRMVVKITDFGCNSILPPKKDLWTAPEHLRQ ATISQKGDVYSFAIIAQEIILRKETFYTLSCRDHNEKIFRVENSYGKPFRPDLFLETADEKELEVYLLVK SCWEEDPEKRPDFKKIESTLAKIFGLFHDQKNESYMDTLIRRLQLYSRNLEHLVEERTQLYKAERDRADH LNFMLLPRLVVKSLKEKGIVEPELYEEVTIYFSDIVGFTTICKYSTPMEVVDMLNDIYKSFDQIVDHHDV YKVETIGDAYVVASGLPMRNGNRHAVDISKMALDILSFIGTFELEHLPGLPVWIRIGVHSGPCAAGVVGI KMPRYCLFGDTVNTASRMESTGLPLRIHMSSSTITILKRTDCQFLYEVRGETYLKGRGTETTYWLTGMKD QEYNLPSPPTVENQQRLQTEFSDMIVSALQKRQASGKKSRRPTRVASYKKGFLEYMQLNNSDHDSTYF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_145067 |
| ORF Size | 3144 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_145067.3, NP_659504.2 |
| RefSeq Size | 3879 bp |
| RefSeq ORF | 3147 bp |
| Locus ID | 14917 |
| UniProt ID | Q3UWA6 |
| MW | 120.9 kDa |
| Gene Summary | Receptor for the E.coli heat-stable enterotoxin (E.coli enterotoxin markedly stimulates the accumulation of cGMP in mammalian cells expressing GC-C). Also activated by the endogenous peptide guanylin (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223348 | Gucy2c (untagged) - Mouse guanylate cyclase 2c (Gucy2c), transcript variant 2, (10ug) |
CNY 13,490.00 |
|
| MG211529 | Gucy2c (tGFP-tagged) - Mouse guanylate cyclase 2c (Gucy2c) |
CNY 8,896.00 |
|
| MR211529L3 | Lenti ORF clone of Gucy2c (Myc-DDK-tagged) - Mouse guanylate cyclase 2c (Gucy2c), transcript variant 2 |
CNY 10,260.00 |
|
| MR211529L4 | Lenti ORF clone of Gucy2c (mGFP-tagged) - Mouse guanylate cyclase 2c (Gucy2c), transcript variant 2 |
CNY 10,260.00 |
