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Trim24 (NM_145076) Mouse Tagged ORF Clone
CAT＃: MR211536
- TrueORF®
Trim24 (Myc-DDK-tagged) - Mouse tripartite motif-containing 24 (Trim24)
ORF Plasmid: tGFP
"NM_145076" in other vectors (4)
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CNY 7,320.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | A130082H20Rik; AI447469; D430004I05Rik; Tif; TIF1; TIF1-alpha; Tif1a; TIF1alpha |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211536 representing NM_145076
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGTGGCTGTGGAGAAGGCGGCGGCGGCAGCGGCTCCGGCCGGAGGCCCCGCAGCGGCGGCGCCGA GCGGGGAGAATGAGGCCGAGAGCCGGCAGGGCCCGGACTCGGAGAGCGGCGGCGAGGCGTCCCGGCTCAA CCTGTTGGACACTTGCGCCGTGTGCCACCAGAACATCCAGAGCCGGGTGCCCAAGCTGCTGCCCTGCCTG CACTCGTTCTGCCAGCGCTGTTTGCCCGCGCCGCAGCGCTATCTCATGCTGACGGCGCCCGCGCTGGGCT CGGCAGAGACCCCTCCACCCGCTCCCGCCCCCGCCCCCGCCCCGGGCTCCCCGGCCGGTGGTCCTTCGCC ATTCGCCACCCAAGTTGGAGTCATTCGATGCCCAGTTTGCAGTCAAGAGTGTGCTGAGAGACACATCATA GACAACTTTTTTGTGAAGGACACCACTGAAGTTCCTAGTAGTACAGTAGAAAAGTCTAATCAGGTATGTA CAAGCTGTGAAGACAATGCAGAAGCTAATGGGTTTTGTGTAGAGTGTGTTGAATGGCTCTGCAAGACATG TATTAGAGCTCACCAGAGGGTGAAGTTCACAAAAGACCACACAGTCAGGCAGAAAGAAGAAGTATCTCCA GAGGCAGTTGGGGTGACCAGTCAGCGACCAGTGTTTTGTCCCTTCCATAAAAAGGAGCAGTTGAAACTTT ACTGTGAAACATGTGATAAACTGACCTGTCGAGACTGCCAGCTGCTAGAACACAAAGAACACAGGTATCA ATTTATAGAAGAAGCTTTTCAGAATCAAAAAGTGATCATAGATACTCTAATCACCAAACTGATGGAAAAA ACAAAATATATAAAGTATACAGGAAATCAGATCCAAAATAGGATAATTGAAATAAATCAAAACCAAAAGC AGGTGGAACAGGATATTAAAGTTGCCATCTTCACATTGATGGTGGAGATAAACAAAAAAGGGAAAGCTCT GCTGCACCAGCTTGAGAGTCTTGCAAAGGACCATCGAATGAAACTCATGCAACAACAGCAGGAAGTGGCT GGGCTTTCTAAGCAGTTAGAGCACGTCATGCATTTTTCTAAATGGGCTGTTTCCAGTGGCAGCAGCACAG CCTTGCTGTACAGCAAGCGGCTGATTACATACAGGTTACGGCACCTTCTTCGTGCAAGGTGTGATGCTTC TCCTGTGACCAACACCACCATCCAGTTTCACTGTGATCCTAGTTTCTGGGCTCAAAATATTATCAACTTG GGTTCTTTAGTAATCGAGGATAAAGAGAGCCAGCCACAAATGCCTAAGCAGAATCCTGTCGTGGAGCAGA GTTCACAGCCACCAGGTGGTTTACCTTCCAACCAGTTATCCAAGTTCCCAACACAGATCAGCCTAGCTCA GTTACGACTCCAGCATATTCAGCAACAGGTAATGGCTCAGAGGCAACAGGTGCAACGGAGGCCAGCACCT GTGGGTTTACCAAACCCTAGAATGCAGGGGCCCATCCAGCAGCCTTCCATCTCTCATCAGCATCCGCCAC CACGCTTAATAAACTTTCAGAATCACAGCCCTAAGCCCAATGGACCAGTTCTTCCTCCTTATCCTCAGCA GCTGAGATATTCACCAAGCCAGAATGTACCTCGGCAGACAACAATAAAGCCCAACCCCTTGCAAATGGCT TTTTTGGCTCAACAGGCCATAAAACAGTGGCAGATCAGCAGTGTACAGGCTCCGCCCACAACTGCCAGCA GCTCCTCCTCCACGCCGTCCAGCCCCACAATCACAAGTGCAGCTGGGTACGATGGAAAAGCTTTTAGTTC ACCCATGATTGATCTGAGTGCACCGGTGGGAGGGTCTTACAATCTTCCTTCTCTTCCAGATATTGATTGT TCAAGTACTATAATGTTGGACAACATTGCAAGGAAAGACACAGGTGTAGATCACGCCCAGCCGAGGCCTC CGTCAAACAGAACGGTGCAGTCACCAAATTCATCAGTGCCATCTCCAGGCCTTGCAGGGCCTGTTACTAT GACTAGCGTCCATCCCCCAATACGTTCACCTAGTGCCTCCAGTGTTGGAAGTCGAGGAAGCTCTGGCTCT TCCAGCAAACCAGCAGGAGCTGATTCTACTCACAAGGTCCCAGTAGTCATGCTGGAGCCAATTCGAATAA AACAGGAAAACAGTGGACCACCTGAAAATTATGATTTTCCTGTTGTTATAGTAAAACAAGAATCAGATGA AGAATCTAGACCTCAAAATACTAACTATCCAAGAAGCATACTTACCTCCCTCCTCTTAAACAGCAGTCAG AGCTCTGCTTCTGAGGAAACCGTGTTACGATCTGATGCCCCTGATAGTACAGGAGATCAGCCTGGACTCC ATCAAGAAAATTCCTCAAATGGAAAGTCTGAGTGGTCGGATGCCTCCCAGAAGTCCCCTGTGCATGTCGG AGAGACGAGGAAGGAGGATGACCCCAATGAAGACTGGTGTGCTGTTTGTCAAAATGGTGGGGAACTCCTA TGCTGTGAGAAATGTCCTAAAGTATTCCATCTTACTTGTCATGTGCCCACCTTGACAAATTTTCCAAGTG GAGAATGGATCTGTACTTTCTGCCGAGACTTATCTAAGCCAGAGGTTGACTATGATTGTGATGTTCCCAG TCACCACTCAGAGAAACGGAAAAGTGAAGGCCTTACTAAGTTAACGCCAATAGACAAAAGGAAATGTGAA CGCCTACTTCTGTTTCTTTACTGCCATGAAATGAGCCTGGCTTTCCAAGACCCTGTTCCTCTAACTGTGC CTGATTATTATAAAATAATTAAAAACCCAATGGACTTGTCAACCATCAAGAAAAGACTTCAGGAGGATTA TTGCATGTATACAAAGCCTGAAGACTTTGTAGCTGATTTTAGATTGATCTTTCAAAACTGTGCTGAATTC AATGAGCCTGATTCTGAAGTAGCCAATGCTGGTATAAAACTTGAAAGCTATTTTGAAGAACTTCTAAAGA ATCTTTATCCAGAAAAAAGGTTTCCTAAGGTAGAATTCAGGCATGAAGCAGAAGACTGTAAGTTCAGTGA CGACTCAGACGATGACTTTGTACAGCCCCGGAAGAAGCGTCTCAAGAGCACCGAGGATCGCCAGCTGCTT AAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211536 representing NM_145076
Red=Cloning site Green=Tags(s) MEVAVEKAAAAAAPAGGPAAAAPSGENEAESRQGPDSESGGEASRLNLLDTCAVCHQNIQSRVPKLLPCL HSFCQRCLPAPQRYLMLTAPALGSAETPPPAPAPAPAPGSPAGGPSPFATQVGVIRCPVCSQECAERHII DNFFVKDTTEVPSSTVEKSNQVCTSCEDNAEANGFCVECVEWLCKTCIRAHQRVKFTKDHTVRQKEEVSP EAVGVTSQRPVFCPFHKKEQLKLYCETCDKLTCRDCQLLEHKEHRYQFIEEAFQNQKVIIDTLITKLMEK TKYIKYTGNQIQNRIIEINQNQKQVEQDIKVAIFTLMVEINKKGKALLHQLESLAKDHRMKLMQQQQEVA GLSKQLEHVMHFSKWAVSSGSSTALLYSKRLITYRLRHLLRARCDASPVTNTTIQFHCDPSFWAQNIINL GSLVIEDKESQPQMPKQNPVVEQSSQPPGGLPSNQLSKFPTQISLAQLRLQHIQQQVMAQRQQVQRRPAP VGLPNPRMQGPIQQPSISHQHPPPRLINFQNHSPKPNGPVLPPYPQQLRYSPSQNVPRQTTIKPNPLQMA FLAQQAIKQWQISSVQAPPTTASSSSSTPSSPTITSAAGYDGKAFSSPMIDLSAPVGGSYNLPSLPDIDC SSTIMLDNIARKDTGVDHAQPRPPSNRTVQSPNSSVPSPGLAGPVTMTSVHPPIRSPSASSVGSRGSSGS SSKPAGADSTHKVPVVMLEPIRIKQENSGPPENYDFPVVIVKQESDEESRPQNTNYPRSILTSLLLNSSQ SSASEETVLRSDAPDSTGDQPGLHQENSSNGKSEWSDASQKSPVHVGETRKEDDPNEDWCAVCQNGGELL CCEKCPKVFHLTCHVPTLTNFPSGEWICTFCRDLSKPEVDYDCDVPSHHSEKRKSEGLTKLTPIDKRKCE RLLLFLYCHEMSLAFQDPVPLTVPDYYKIIKNPMDLSTIKKRLQEDYCMYTKPEDFVADFRLIFQNCAEF NEPDSEVANAGIKLESYFEELLKNLYPEKRFPKVEFRHEAEDCKFSDDSDDDFVQPRKKRLKSTEDRQLL K TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_145076 |
| ORF Size | 3153 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_145076.4, NP_659542.3 |
| RefSeq Size | 6191 bp |
| RefSeq ORF | 3156 bp |
| Locus ID | 21848 |
| UniProt ID | Q64127 |
| MW | 117.1 kDa |
| Gene Summary | The protein encoded by this gene is part of the tripartite-motif containing family (TRIM), which are typified by the RING, B-box type 1, B-box type 2, and coiled-coil region domains. This protein, which also contains a PHD/TTC finger and bromodomain important for regulating nuclear receptors and binding chromatin, has important roles in differentiation, development, and tissue homeostasis. This protein has been reported to regulate the activity of the tumor suppressor p53 and of the retinoic acid receptor. A translocation event between this gene and Braf transforming gene, which results in the fusion protein T18, has been reported in hepatocellular carcinomas. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jan 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223351 | Trim24 (untagged) - Mouse tripartite motif-containing 24 (Trim24), (10ug) |
CNY 7,328.00 |
|
| MG211536 | Trim24 (tGFP-tagged) - Mouse tripartite motif protein 24 (Trim24) |
CNY 10,170.00 |
|
| MR211536L3 | Lenti ORF clone of Trim24 (Myc-DDK-tagged) - Mouse tripartite motif-containing 24 (Trim24) |
CNY 11,120.00 |
|
| MR211536L4 | Lenti ORF clone of Trim24 (mGFP-tagged) - Mouse tripartite motif-containing 24 (Trim24) |
CNY 11,120.00 |
