Lrrk1 (BC072664) Mouse Tagged ORF Clone
CAT#: MR211710
- TrueORF®
Lrrk1 (Myc-DDK-tagged) - Mouse leucine-rich repeat kinase 1 (cDNA clone MGC:99993 IMAGE:30543489)
ORF Plasmid: tGFP
"BC072664" in other vectors (4)
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CNY 11,500.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | MGC28646, mKIAA1790 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR211710 representing BC072664
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCAGCAAACTGTCTTTACCAGGTGGCAGCTCCACGGTGCAGTGCCCCAGCATGGAAGAAATCCACA CTGCGTACAAGCAGAGGAACCTCTCCCGAGCCCGAGACCTACTCAGAGGGGTTTGTGAAGAGAGCGAATC CTCACAGGAGAAGGGCCAGCTGCTGAGCATCGCGGCAGCCCACGGGGATCTGGAGACTGTTCAGTTCCTG CTCACTGAGAAGCGCGTGGAGCTGCCGACGGAGCCCACCGATGACAACCCAGCTGTGGTGGCAGCGCATT TTGGACACGCCGAAGTAGTACGTGAACTACTAGAGTCCTTGCCAGGTCCCTGTACCCCCCAGCGGCTACT GAACTGGATGCTGGCCCTGGCTTGCCAGCGAGGGCACCTGGAGGTTGTGAAGCTGCTGGTCCTGACACAT GGGGCTGACCCGGAGAACTATGCCGTCAGGAAGAATGAGTTCCCCGTCATTGTGCGGTTGCCCCTCTATG CGGCCATCAAGGCAGGGAATGAAGACATTGCCATATTCCTGCTTCGGCACGGAGCCTATTTCTGCTCCTA CATCCTGCTGGATAGTCCTGAGCCAAGCAAACATCTGCTCAGGAAGTATTTCATCGAAGCCAGCGCCCTG CCCAGCAGCTATCCCGGGAAAATCGCACTTCGTGTGAAATGGTCCCATCTGAAGTTGCCCTGGGTGGACC TGGACTGGCTCCTAGACATCTCCTGCCAGATCACAGAGCTTGACCTGTCTGCCAACTGCCTGCCTTCGCT CCCTTCCATCATCCCTTGGGGACTCATCAATCTAAAGAAGCTGAATCTCTCAAACAACCAGCTGGGGGAG CTGCCCTGCGTGCAGTCCTCCGACGAAATCATCTGCTCCAGGTTACTTGAAATTGACATCTCCAGCAACA AACTGTCTCACCTCCCTCCTGGATTCCTGCACCTCTCAAAACTTCAAAAGCTGACTGCTTCCAAAAACTA CCTGGAGCGGCTGTTTGAAGAAGAAAACGCCACTAACTGGATCGGCCTGCGGAAGCTGCAAGAACTCGAT CTAGCTGACAACAGGCTGACAGAGCTCCCTGTCCAATTTATGCACTCCTTCAAGTCTCTCACCTCTCTGA ATGTCTCCAGGAACAACCTGAAGAGCTTCCCGGATCCCTGGTCCTGCCCTTTGAAATGTTGTAAAGCCTC CAAAAATGCCCTGGAATCGCTGCCAGACAAAATGGCTGTCTTTTGGAAAAGCCACCTTCGGGATGCAGAT TTCTCTGAGAACTCTCTGAAAGAGGTGCCCCTGGGATTGTTCCAGCTTGACGCCCTCATGTTCTTGAGGT TACAGGGAAACCAGCTGTTGTCGCTGCCACCTCAAGAGAAGTGGACCTGCACCCAACTCAAGACCCTGGA CCTCTCCAGAAACCAGCTCGGCAAAAATGAAGATGGGCTTAAAACAAAGAGGATCTCCCTGTTCACCACC AGAGGGCGCCAGCGTTCTGGAACTGAGACAGCCTCCATGTTGGAATTCCCAGCTTTCCTAAGTGAGTCTT TGGAGGTCCTTTGTCTGAATGACAACCATCTTGATGCTGTTCCCCCATCGGTCTGCCTGCTGAAGAACCT CTCAGAGCTCTACTTGGGCAATAACCCTGGCCTCCGAGAGCTCCCTCCGGAGCTAGGCCAGCTGGGGAAC CTCTGGCAACTGGACATTGAAGATCTGAACATTAGCAACGTGCCTGCGGAGGTCAGGAAAGAAGGCCCGA AAGCAACACTGTCTTTCCTGCGTGCTCAGCTGCGCAAAGCAGAGAAGTGTAAGCTGATGAAGATGATCCT CGTGGGTCCCCCGCGCCAGGGCAAGTCCACACTCCTGGAGATCTTACAGACCGGGAAGGCCCCGCAGCTA GCACACAGTGAAGCCACCATCAGGACCACCAAGTGGGAGCTCCAGAGACCAGCAGGCTCCAAAGCCAAGG TTGAGTCCGTAGAGTTCAACGTCTGGGACATCGGGGGTCCGGCCAGCATGGCCACGGTCAATCAGTGCTT CTTCACAGACAAGGCGCTGTATGTGGTGGTCTGGAACCTGGCACTGGGAGAGGAAGCCGTGGCCAACCTC CAGTTCTGGCTGCTCAACATTGAGGCCAAGGCCCCAAATGCCGTTGTGCTGGTGGTCGGGACACACCTGG ACCTAATCGAAGCTAAGTTCCGAGTGGAGAGGATAGCAACCCTGCGTGCCTACGTGCTGGCCCTCTGCCG CTCGCCATCGGGCTCCAGGGCTACGGGCTTCCCGGACATCACTTTCAAACACTTGCATGAGATCTCCTGC AAGAATCTGGAAGGGCAGGAGGGGCTGCGGCAACTGATCTTCCACGTCACGTGCAACATGAAGGATGTGG GTAGCACTATCGGCTGTCAAAAACTCGCCGGCAGGCTGATCCCCAGGAGCTACATAAGCCTACAGGAGGC CGTGCTGGCTGAGCAACAGCGCCGCAGTCTGGGTGACCAAGTACAGTATCTGACGGACAGGCAGCTGGAC CAGCTGGTAGAGCAGACGCCTGGCAATGATATCAAAGACTATGAAGACCTGCAGTCTGCCATCAGCTTCC TCATAGAAACTGGGACCCTGCTGCACTTTCCAGACACGAGCCATGGCCTAAGGAACCTCTACTTCCTGGA TCCCATCTGGCTCTCTGAATGCCTGCAGAGGATCTTTAACATCAAGGGCTCGCGGTCAGTGGCAAAGAAT GGGGTGATCCAAGCGGAAGACCTCAGGATGCTGCTGGTGGGGACAGGCTTCACACAGCAGACTGAGGAGC AATACTTCCAGTTCCTTGCCAAGTTCGAGATTGCCCTCCCTGTTGCCAATGACAGCTACCTCCTACCACA CCTCCTTCCATCCAAGCCTGGGCTGGACACCCACAGCATGCGGCACCCAATGGCTAACACCATCCAGCGG GTGTTTAAGATGAGCTTCGTGCCTGTTGGCTTCTGGCAACGGTTCATAGCACGGATGCTAATCAGCTTGG CTGAGATGGACCTGCAGCTTTTTGAAAACAAGAAGAATACGAAAAGCAGGAACCGGAAAGTCACTATTTA CAGTTTTACAGGGAGCCAGAGAAACCGCTGCAGTACATTCCGAGTCCGAAGAAATCAGACCATCTACTGG CAGGAAGGGCTGCTGGTCACTTTCGATGGGGGCTACCTCAGTGTGGAATCCTCAGATGTGAACTGGAAAA AGAAAAAAAGCGGAGGAATTAAAATCATCTGCCAGTCAGAGATGAGGGACTTCTCGGCAATGGCTTTTAT CACAGACCATGTCAACTCCCTGATTGACCAGTGGTTCCCTGCCCTGACGGCCACAGAGTGGTGCCTGGCT GTCTGGAGGGGCTGGGGCGCCAGGGAGTTTGACATCTTCTACCAGCTCCCTCACCAGCTATTCCAGTGTG CCTTTCTCTGCCAACTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211710 representing BC072664
Red=Cloning site Green=Tags(s) MGSKLSLPGGSSTVQCPSMEEIHTAYKQRNLSRARDLLRGVCEESESSQEKGQLLSIAAAHGDLETVQFL LTEKRVELPTEPTDDNPAVVAAHFGHAEVVRELLESLPGPCTPQRLLNWMLALACQRGHLEVVKLLVLTH GADPENYAVRKNEFPVIVRLPLYAAIKAGNEDIAIFLLRHGAYFCSYILLDSPEPSKHLLRKYFIEASAL PSSYPGKIALRVKWSHLKLPWVDLDWLLDISCQITELDLSANCLPSLPSIIPWGLINLKKLNLSNNQLGE LPCVQSSDEIICSRLLEIDISSNKLSHLPPGFLHLSKLQKLTASKNYLERLFEEENATNWIGLRKLQELD LADNRLTELPVQFMHSFKSLTSLNVSRNNLKSFPDPWSCPLKCCKASKNALESLPDKMAVFWKSHLRDAD FSENSLKEVPLGLFQLDALMFLRLQGNQLLSLPPQEKWTCTQLKTLDLSRNQLGKNEDGLKTKRISLFTT RGRQRSGTETASMLEFPAFLSESLEVLCLNDNHLDAVPPSVCLLKNLSELYLGNNPGLRELPPELGQLGN LWQLDIEDLNISNVPAEVRKEGPKATLSFLRAQLRKAEKCKLMKMILVGPPRQGKSTLLEILQTGKAPQL AHSEATIRTTKWELQRPAGSKAKVESVEFNVWDIGGPASMATVNQCFFTDKALYVVVWNLALGEEAVANL QFWLLNIEAKAPNAVVLVVGTHLDLIEAKFRVERIATLRAYVLALCRSPSGSRATGFPDITFKHLHEISC KNLEGQEGLRQLIFHVTCNMKDVGSTIGCQKLAGRLIPRSYISLQEAVLAEQQRRSLGDQVQYLTDRQLD QLVEQTPGNDIKDYEDLQSAISFLIETGTLLHFPDTSHGLRNLYFLDPIWLSECLQRIFNIKGSRSVAKN GVIQAEDLRMLLVGTGFTQQTEEQYFQFLAKFEIALPVANDSYLLPHLLPSKPGLDTHSMRHPMANTIQR VFKMSFVPVGFWQRFIARMLISLAEMDLQLFENKKNTKSRNRKVTIYSFTGSQRNRCSTFRVRRNQTIYW QEGLLVTFDGGYLSVESSDVNWKKKKSGGIKIICQSEMRDFSAMAFITDHVNSLIDQWFPALTATEWCLA VWRGWGAREFDIFYQLPHQLFQCAFLCQL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | BC072664 |
ORF Size | 3447 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | BC072664.1 |
RefSeq Size | 5284 bp |
RefSeq ORF | 3449 bp |
Locus ID | 233328 |
MW | 193.7 kDa |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC223129 | Lrrk1 (untagged) - Mouse leucine-rich repeat kinase 1 (cDNA clone MGC:99993 IMAGE:30543489), (10ug) |
CNY 12,920.00 |
|
MG211710 | Lrrk1 (tGFP-tagged) - Mouse leucine-rich repeat kinase 1 (cDNA clone MGC:99993 IMAGE:30543489) |
CNY 12,640.00 |
|
MR211710L3 | Lenti ORF clone of Lrrk1 (Myc-DDK-tagged) - Mouse leucine-rich repeat kinase 1 (cDNA clone MGC:99993 IMAGE:30543489) |
CNY 13,400.00 |
|
MR211710L4 | Lenti ORF clone of Lrrk1 (mGFP-tagged) - Mouse leucine-rich repeat kinase 1 (cDNA clone MGC:99993 IMAGE:30543489) |
CNY 13,400.00 |