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Polr2b (NM_153798) Mouse Tagged ORF Clone
CAT＃: MR211738
- TrueORF®
Polr2b (Myc-DDK-tagged) - Mouse polymerase (RNA) II (DNA directed) polypeptide B (Polr2b)
ORF Plasmid: tGFP
"NM_153798" in other vectors (3)
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CNY 8,184.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Pol2rb; Rpb2; Rpb140 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211738 representing NM_153798
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTATGACGCCGACGAGGACATGCAATATGATGAAGACGACGATGAGATCACTCCGGATCTGTGGCAGG AAGCATGCTGGATCGTGATCAGTTCTTACTTTGATGAGAAAGGCTTGGTCAGACAACAGCTGGATTCCTT TGATGAGTTTATTCAGATGTCTGTTCAAAGAATTGTGGAAGATGCACCACCGATTGACCTACAGGCCGAA GCTCAGCACGCCAGTGGAGAAGTTGAAGAGCCGCCAAGATATTTACTGAAGTTTGAACAAATTTACCTCT CCAAACCTACTCATTGGGAAAGAGATGGTGCCCCTTCGCCAATGATGCCAAATGAAGCCAGGTTAAGAAA TCTCACGTATTCTGCTCCGCTTTACGTTGACATAACAAAGACAGTCATTAAAGAAGGTGAAGAACAGCTG CAGACTCAGCACCAGAAAACGTTCATAGGGAAAATTCCAATTATGCTGCGCTCCACCTACTGCCTGTTAA ATGGCTTAACAGATCGTGACCTGTGTGAGCTGAATGAGTGTCCTTTGGATCCCGGTGGCTATTTTATTAT CAATGGATCAGAAAAGGTTCTGATAGCTCAAGAGAAAATGGCAACAAACACAGTCTATGTGTTTGCCAAA AAGGATTCCAAGTATGCCTATACAGGAGAATGTCGCTCATGCCTGGAGAATTCTTCCCGACCCACCAGTA CTATATGGGTTAGCATGCTGGCCAGAGGAGGACAGGGTGCAAAGAAGAGTGCTATTGGCCAGCGCATCGT GGCAACGTTACCATACATCAAGCAAGAAGTTCCTATCATCATTGTGTTCAGAGCACTAGGCTTTGTGTCT GATAGAGATATTTTAGAACACATCATTTATGACTTCGAGGATCCAGAGATGATGGAAATGGTTAAACCAT CTCTCGATGAAGCGTTTGTCATCCAAGAGCAGAATGTTGCCTTAAACTTCATCGGTTCAAGAGGGGCAAA GCCTGGTGTTACTAAGGAGAAAAGAATTAAATATGCAAAAGAAGTCTTACAGAAAGAAATGCTCCCTCAC GTTGGTGTCAGTGATTTCTGTGAGACCAAAAAGGCCTATTTCTTGGGGTATATGGTTCATAGGTTGCTTC TAGCAGCCCTAGGCAGAAGAGAACTAGACGACAGAGATCACTATGGCAACAAGAGATTGGATCTTGCTGG GCCTCTGCTTGCATTCTTATTCAGAGGCATGTTTAAGAATTTGCTGAAAGAAGTACGAATCTATGCGCAG AAGTTTATTGATCGCGGGAAGGATTTTAACTTAGAGCTGGCAATCAAGACAAGGATCATATCTGATGGGC TAAAATACTCTTTAGCCACTGGGAACTGGGGTGACCAGAAGAAAGCTCATCAAGCCAGAGCTGGAGTATC CCAGGTATTGAACCGCCTGACGTTTGCATCTACTCTTTCTCACCTGCGTCGTCTAAACTCTCCTATTGGC AGAGATGGCAAGCTAGCGAAGCCCAGACAGTTGCATAATACCTTGTGGGGAATGGTGTGTCCTGCTGAAA CTCCAGAGGGCCATGCTGTAGGGCTTGTGAAGAATTTAGCCCTGATGGCTTATATTTCAGTTGGATCTCA ACCCTCTCCAATTTTGGAATTTTTAGAAGAATGGAGCATGGAAAATTTAGAAGAAATTTCTCCTGCAGCT ATTGCTGATGCAACCAAGATTTTTGTTAATGGCTGCTGGGTTGGAATCCATAAAGACCCCGAACAACTCA TGAACACGCTAAGGAAGTTACGGCGGCAGATGGACATCATTGTATCCGAAGTTTCTATGATCAGAGATAT TCGAGAGCGGGAGATTCGGATCTATACAGATGCAGGCCGTATTTGTAGACCCCTTCTGATTGTGGAAAAG CAGAAGCTGCTTTTGAAGAAGAGACACATTGACCAGTTGAAAGAGAGAGAATATAACAACTACAGTTGGC AGGATCTGGTAGCCAGTGGGGTAGTAGAGTATATTGATACCCTGGAAGAGGAGACCGTGATGCTTGCAAT GACTCCAGATGATTTGCAGGAGAAGGAAGTAGCTTATTGTTCTACCTACACCCACTGTGAGATTCACCCA TCAATGATTCTTGGAGTCTGTGCATCTATTATTCCTTTTCCTGATCATAACCAGTCCCCTAGAAACACAT ACCAGTCTGCTATGGGCAAGCAGGCTATGGGAGTTTATATCACCAACTTCCATGTGCGGATGGACACCTT GGCCCATGTTCTCTACTATCCTCAGAAACCCCTTGTGACTACACGGTCTATGGAATATCTACGATTCAGA GAGCTGCCAGCAGGAATCAACTCAATTGTGGCCATTGCATCCTACACGGGCTATAATCAAGAAGACTCTG TTATCATGAATCGTTCAGCTGTGGATCGAGGCTTTTTCAGGTCTGTTTTCTACCGATCATACAAAGAACA AGAGTCTAAAAAGGGATTTGATCAAGAAGAAGTGTTTGAGAAGCCTACACGTGAGACATGCCAGGGAATG AGGCATGCCATCTATGAAAAGTTGGACGATGACGGTTTGATAGCTCCGGGGGTCCGAGTCTCCGGAGATG ACGTTATTATCGGCAAGACAGTCACCTTACCCGAGAACGAGGATGAGCTGGAGAGCACCAACAGGCGCTA CACCAAGAGGGACTGCAGCACTTTCCTCAGAACTAGTGAGACGGGCATCGTGGACCAGGTCATGGTCACT CTCAACCAGGAAGGATATAAATTTTGTAAAATAAGGGTGCGCTCTGTTAGAATTCCGCAGATTGGAGACA AATTTGCTAGCCGACATGGTCAGAAGGGAACTTGTGGTATTCAGTATAGACAAGAGGACATGCCGTTCAC TTGCGAAGGCATAACTCCTGACATCATCATAAACCCCCACGCCATCCCCTCCCGGATGACAATTGGTCAC TTGATCGAATGTCTCCAAGGGAAGGTCTCTGCCAACAAGGGTGAAATTGGTGACGCTACTCCATTTAATG ATGCTGTCAATGTGCAGAAGATTTCTAATCTTTTGTCTGACTATGGCTACCATCTCAGAGGGAACGAGGT GCTCTACAATGGATTCACTGGACGAAAAATCACATCACAGATTTTTATTGGCCCCACTTACTACCAGCGT TTGAAGCATATGGTGGATGACAAAATTCATTCTCGTGCTAGGGGACCCATTCAGATTCTTAACAGACAGC CCATGGAGGGCAGATCTCGTGATGGTGGCCTGCGCTTCGGAGAAATGGAACGAGACTGTCAAATTGCCCA CGGTGCAGCACAGTTTCTAAGAGAAAGACTGTTTGAAGCATCAGATCCGTATCAGGTTCATGTCTGTAAT CTTTGTGGAATAATGGCAATTGCTAACACGAGGACTCATACATATGAGTGCAGGGGATGCCGCAATAAAA CCCAGATTTCCTTGGTGCGAATGCCGTACGCGTGCAAGCTGTTGTTCCAGGAGCTCATGTCCATGAGCAT CGCACCACGAATGATGAGTGTT AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >MR211738 representing NM_153798
Red=Cloning site Green=Tags(s) MYDADEDMQYDEDDDEITPDLWQEACWIVISSYFDEKGLVRQQLDSFDEFIQMSVQRIVEDAPPIDLQAE AQHASGEVEEPPRYLLKFEQIYLSKPTHWERDGAPSPMMPNEARLRNLTYSAPLYVDITKTVIKEGEEQL QTQHQKTFIGKIPIMLRSTYCLLNGLTDRDLCELNECPLDPGGYFIINGSEKVLIAQEKMATNTVYVFAK KDSKYAYTGECRSCLENSSRPTSTIWVSMLARGGQGAKKSAIGQRIVATLPYIKQEVPIIIVFRALGFVS DRDILEHIIYDFEDPEMMEMVKPSLDEAFVIQEQNVALNFIGSRGAKPGVTKEKRIKYAKEVLQKEMLPH VGVSDFCETKKAYFLGYMVHRLLLAALGRRELDDRDHYGNKRLDLAGPLLAFLFRGMFKNLLKEVRIYAQ KFIDRGKDFNLELAIKTRIISDGLKYSLATGNWGDQKKAHQARAGVSQVLNRLTFASTLSHLRRLNSPIG RDGKLAKPRQLHNTLWGMVCPAETPEGHAVGLVKNLALMAYISVGSQPSPILEFLEEWSMENLEEISPAA IADATKIFVNGCWVGIHKDPEQLMNTLRKLRRQMDIIVSEVSMIRDIREREIRIYTDAGRICRPLLIVEK QKLLLKKRHIDQLKEREYNNYSWQDLVASGVVEYIDTLEEETVMLAMTPDDLQEKEVAYCSTYTHCEIHP SMILGVCASIIPFPDHNQSPRNTYQSAMGKQAMGVYITNFHVRMDTLAHVLYYPQKPLVTTRSMEYLRFR ELPAGINSIVAIASYTGYNQEDSVIMNRSAVDRGFFRSVFYRSYKEQESKKGFDQEEVFEKPTRETCQGM RHAIYEKLDDDGLIAPGVRVSGDDVIIGKTVTLPENEDELESTNRRYTKRDCSTFLRTSETGIVDQVMVT LNQEGYKFCKIRVRSVRIPQIGDKFASRHGQKGTCGIQYRQEDMPFTCEGITPDIIINPHAIPSRMTIGH LIECLQGKVSANKGEIGDATPFNDAVNVQKISNLLSDYGYHLRGNEVLYNGFTGRKITSQIFIGPTYYQR LKHMVDDKIHSRARGPIQILNRQPMEGRSRDGGLRFGEMERDCQIAHGAAQFLRERLFEASDPYQVHVCN LCGIMAIANTRTHTYECRGCRNKTQISLVRMPYACKLLFQELMSMSIAPRMMSV SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_153798 |
| ORF Size | 3525 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_153798.2, NP_722493.2 |
| RefSeq Size | 3812 bp |
| RefSeq ORF | 3525 bp |
| Locus ID | 231329 |
| UniProt ID | Q8CFI7 |
| MW | 133.9 kDa |
| Gene Summary | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB2 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG211738 | Polr2b (tGFP-tagged) - Mouse polymerase (RNA) II (DNA directed) polypeptide B (Polr2b) |
CNY 11,400.00 |
|
| MR211738L3 | Lenti ORF clone of Polr2b (Myc-DDK-tagged) - Mouse polymerase (RNA) II (DNA directed) polypeptide B (Polr2b) |
CNY 12,260.00 |
|
| MR211738L4 | Lenti ORF clone of Polr2b (mGFP-tagged) - Mouse polymerase (RNA) II (DNA directed) polypeptide B (Polr2b) |
CNY 12,260.00 |
