Hipk2 (BC031904) Mouse Tagged ORF Clone

CAT#: MR211742

  • TrueORF®

Hipk2 (Myc-DDK-tagged) - Mouse homeodomain interacting protein kinase 2 (cDNA clone MGC:36065 IMAGE:5368577)

ORF Plasmid: DDK tGFP



  "BC031904" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 11,690.00


货期*
2周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms 1110014O20Rik; B230339E18Rik; Stank
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR211742 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCCCGTGTACGAAGGTATGGCCTCACATGTGCAAGTTTTCTCCCCTCACACCCTTCAATCAAGTG
CCTTCTGTAGTGTGAAGAAACTAAAAGTAGAGCCAAGTTCCAACTGGGACATGACTGGGTACGGCTCCCA
CAGCAAAGTGTACAGCCAGAGCAAGAACATACCACCTTCTCAGCCAGCCTCCACAACCGTCAGCACCTCC
TTGCCAATCCCCAACCCAAGCCTACCTTACGAGCAGACCATCATCTTCCCAGGAAGTACTGGACACATAG
TTGTAACATCAGCAAGTAGCACTTCTGTCACCGGGCAAGTCCTCGGTGGACCACATAACCTAATGCGTCG
AAGCACTGTGAGCCTCCTTGACACCTACCAAAAATGTGGACTCAAGCGCAAGAGTGAGGAGATTGAGAAC
ACAAGCAGCGTGCAGATCATTGAAGAGCATCCACCCATGATTCAGAATAATGCCAGCGGGGCCACTGTAG
CCACTGCCACCACATCCACTGCCACCTCCAAAAACAGTGGCTCCAACAGTGAAGGGGACTATCAGCTGGT
GCAGCATGAAGTACTGTGCTCCATGACCAACACCTATGAGGTTTTAGAGTTCTTGGGGCGAGGGACATTT
GGGCAAGTGGTCAAGTGCTGGAAACGGGGCACCAATGAAATTGTGGCCATTAAGATCCTCAAGAACCACC
CCTCCTATGCCCGGCAAGGCCAGATTGAAGTGAGCATCTTGGCCCGGCTGAGCACGGAGAGCGCTGATGA
CTACAATTTTGTGCGGGCCTATGAGTGCTTCCAGCACAAGAACCACACGTGCTTAGTCTTTGAGATGTTG
GAGCAGAACCTTTATGACTTTCTGAAACAGAACAAGTTTAGTCCCTTGCCCCTCAAGTATATTCGCCCAG
TCCTCCAGCAGGTAGCCACAGCCCTGATGAAACTCAAAAGCCTGGGTCTTATCCATGCTGACCTCAAACC
AGAAAACATCATGCTGGTGGATCCATCCAGACAACCGTACCGAGTGAAGGTCATTGACTTTGGTTCAGCT
AGTCATGTGTCCAAAGCTGTCTGCTCTACGTACTTGCAATCCAGATACTACCGGGCCCCTGAGATCATCC
TTGGTTTGCCATTCTGTGAGGCAATTGACATGTGGTCCCTTGGTTGTGTCATTGCTGAACTGTTCCTGGG
CTGGCCATTATACCCAGGTGCTTCTGAGTATGATCAGATTCGGTATATTTCACAAACACAGGGTTTACCA
GCTGAATATTTATTAAGTGCAGGGACAAAGACAACTAGGTTTTTCAACCGTGACACAGACTCACCGTATC
CTTTGTGGAGGCTAAAGACACCAGATGACCATGAAGCAGAGACGGGAATTAAGTCAAAGGAAGCAAGAAA
GTACATTTTCAACTGTTTGGATGATATGGCCCAGGTGAACATGACAACAGATTTGGAAGGGAGTGACATG
TTAGTAGAGAAGGCAGACCGACGGGAGTTCATTGACCTGTTAAAGAAGATGCTGACCATCGATGCTGATA
AGAGAGTCACTCCCATTGAAACTCTGAACCACCCCTTTGTCACCATGACACACCTGCTTGACTTCCCCCA
TAGTGCCCATGTCAAATCTTGTTTCCAAAACATGGAGATTTGCAAGCGCCGGGTGAATATGTATGACACA
GTGAACCAGAGCAAAACACCTTTCATCACTCACGTGGCCCCCAGCACATCCACCAACTTGACCATGACCT
TTAACAACCAGCTGACCACTGTCCACAACCAGCCCTCAGCGGCATCCATGGCCGCGGTGGCCCCGCGGAG
CATGCCCCTGCAGACGGGAACAGCCCAAATTTGTGCCCGACCTGATCCCTTCCAGCAAGCTCTCATCGTG
TGTCCCCCTGGCTTCCAAGGCCTGCAGGCCTCTCCCTCCAAGCACGCTGGCTACTCAGTGCGGATGGAAA
ATGCTGTCCCCATCGTCACCCAGGCGCCAGGAGCTCAGCCTCTTCAGATCCAACCAGGCCTGCTTGCTCA
GCAGGCCTGGCCAGGTGGGGCCCAACAGATCCTACTTCCTCCAGCATGGCAGCAGCTGACTGGCGTGGCC
ACCCACACATCTGTACAGCATGCAGCTGTGATTCCTGAGACCATGGCAGGCACTCAGCAACTGGCTGACT
GGAGGAACACGCATGCTCATGGAAGTCATTACAATCCCATCATGCAGCAGCCTGCACTCTTGACTGGTCA
TGTGACCCTTCCAGCTGCCCAGCCCTTAAATGTGGGTGTAGCCCATGTGATGAGGCAACAACCAACCAGC
ACCACCTCCTCCAGGAAGAGTAAGCAGCACCAGTCATCTGTGAGAAATGTCTCCACCTGTGAGGTGACCT
CTTCACAGGCTATCAGCTCCCCTCAGCGATCCAAGCGTGTCAAGGAGAACACTCCCCCACGGTGCGCCAT
GGTACACAGCAGCCCAGCTTGCAGCACCTCAGTCACCTGTGGGTGGGGCGACGTGGCCTCCAGCACCACC
CGGGAGCGACAGCGGCAGACGATTGTCATCCCCGACACCCCGAGCCCCACAGTCAGTGTCATCACCATCA
GCAGTGACACCGATGAAGAAGAGGAGCAGAAGCACGCCCCCACCAGCACAGTCTCCAAGCAAAGAAAAAA
TGTCATCAGCTGTGTCACCGTCCACGACTCTCCCTACTCTGACTCCTCCAGCAACACCAGCCCCTACTCG
GTGCAGCAGCGCACAGGGCACAACGGCACCAACACCTTGGACACCAAGGGGGGCCTAGAGAATCACTGCA
CTGGCAACCCCCGCACCATCATCGTACCCCCACTGAAGACCCAGGCCAGTGAAGTGTTGGTAGAATGTGA
CAGCCTAGGGCCAGCGATCAGTGCCAGTCACCATTCGTCCTCCTTCAAGTCCAAGTCCTCCAGCACCGTG
ACCTCCACCAGTGGACACTCTTCAGGGAGCTCTTCAGGAGCCATCGCCTACCGTCAGCAGCGGCCAGGCC
CCCACTTCCAGCAGCAGCAGCCCCTCAATCTCAGCCAGGCCCAGCAGCACATGGCTGCGGACCGCACCGG
GAGTCACCGTCGACAGCAGGCCTACATCACTCCGACCATGGCGCAAGCTCCGTACACCTTCCCGCACAAC
AGCCCCAGCCATGGCACTGTTCACCCCCACCTGGCTGCGGCTGCCCACCTCCCCACCCAGCCTCACCTCT
ACACCTATACAGCGCCCACAGCCCTAGGCTCCACCGGCACTGTAGCCCACCTGGTGGCATCCCAAGGCTC
AGCACGCCACACCGTGCAGCACACTGCCTATCCGGCCAGCATCGTCCACCAGGTCCCTGTGAGCATGGGG
CCCCGGGTCCTGCCCTCGCCCACCATCCACCCCAGTCAGTATCCAGCCCAGTTTGCCCACCAGACCTATA
TCAGCGCCTCGCCAGCCTCCACCGTCTACACTGGATACCCACTGAGTCCTGCCAAGGTCAACCAATACCC
TTACATA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR211742 protein sequence
Red=Cloning site Green=Tags(s)

MAPVYEGMASHVQVFSPHTLQSSAFCSVKKLKVEPSSNWDMTGYGSHSKVYSQSKNIPPSQPASTTVSTS
LPIPNPSLPYEQTIIFPGSTGHIVVTSASSTSVTGQVLGGPHNLMRRSTVSLLDTYQKCGLKRKSEEIEN
TSSVQIIEEHPPMIQNNASGATVATATTSTATSKNSGSNSEGDYQLVQHEVLCSMTNTYEVLEFLGRGTF
GQVVKCWKRGTNEIVAIKILKNHPSYARQGQIEVSILARLSTESADDYNFVRAYECFQHKNHTCLVFEML
EQNLYDFLKQNKFSPLPLKYIRPVLQQVATALMKLKSLGLIHADLKPENIMLVDPSRQPYRVKVIDFGSA
SHVSKAVCSTYLQSRYYRAPEIILGLPFCEAIDMWSLGCVIAELFLGWPLYPGASEYDQIRYISQTQGLP
AEYLLSAGTKTTRFFNRDTDSPYPLWRLKTPDDHEAETGIKSKEARKYIFNCLDDMAQVNMTTDLEGSDM
LVEKADRREFIDLLKKMLTIDADKRVTPIETLNHPFVTMTHLLDFPHSAHVKSCFQNMEICKRRVNMYDT
VNQSKTPFITHVAPSTSTNLTMTFNNQLTTVHNQPSAASMAAVAPRSMPLQTGTAQICARPDPFQQALIV
CPPGFQGLQASPSKHAGYSVRMENAVPIVTQAPGAQPLQIQPGLLAQQAWPGGAQQILLPPAWQQLTGVA
THTSVQHAAVIPETMAGTQQLADWRNTHAHGSHYNPIMQQPALLTGHVTLPAAQPLNVGVAHVMRQQPTS
TTSSRKSKQHQSSVRNVSTCEVTSSQAISSPQRSKRVKENTPPRCAMVHSSPACSTSVTCGWGDVASSTT
RERQRQTIVIPDTPSPTVSVITISSDTDEEEEQKHAPTSTVSKQRKNVISCVTVHDSPYSDSSSNTSPYS
VQQRTGHNGTNTLDTKGGLENHCTGNPRTIIVPPLKTQASEVLVECDSLGPAISASHHSSSFKSKSSSTV
TSTSGHSSGSSSGAIAYRQQRPGPHFQQQQPLNLSQAQQHMAADRTGSHRRQQAYITPTMAQAPYTFPHN
SPSHGTVHPHLAAAAHLPTQPHLYTYTAPTALGSTGTVAHLVASQGSARHTVQHTAYPASIVHQVPVSMG
PRVLPSPTIHPSQYPAQFAHQTYISASPASTVYTGYPLSPAKVNQYPYI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC031904
ORF Size 3507 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC031904, AAH31904
RefSeq Size 3867 bp
RefSeq ORF 3509 bp
Locus ID 15258
MW 127.7 kDa
Gene Summary Serine/threonine-protein kinase involved in transcription regulation, p53/TP53-mediated cellular apoptosis and regulation of the cell cycle. Acts as a corepressor of several transcription factors, including SMAD1 and POU4F1/Brn3a and probably NK homeodomain transcription factors. Phosphorylates PDX1, ATF1, PML, p53/TP53, CREB1, CTBP1, CBX4, RUNX1, EP300, CTNNB1, HMGA1 and ZBTB4. Inhibits cell growth and promotes apoptosis through the activation of p53/TP53 both at the transcription level and at the protein level (by phosphorylation and indirect acetylation). The phosphorylation of p53/TP53 may be mediated by a p53/TP53-HIPK2-AXIN1 complex. Involved in the response to hypoxia by acting as a transcriptional co-suppressor of HIF1A. Mediates transcriptional activation of TP73. In response to TGFB, cooperates with DAXX to activate JNK. Negative regulator through phosphorylation and subsequent proteasomal degradation of CTNNB1 and the antiapoptotic factor CTBP1. In the Wnt/beta-catenin signaling pathway acts as an intermediate kinase between MAP3K7/TAK1 and NLK to promote the proteasomal degradation of MYB. Phosphorylates CBX4 upon DNA damage and promotes its E3 SUMO-protein ligase activity. Activates CREB1 and ATF1 transcription factors by phosphorylation in response to genotoxic stress. In response to DNA damage, stabilizes PML by phosphorylation. PML, HIPK2 and FBXO3 may act synergically to activate p53/TP53-dependent transactivation. Promotes angiogenesis, and is involved in erythroid differentiation, especially during fetal liver erythropoiesis. Phosphorylation of RUNX1 and EP300 stimulates EP300 transcription regulation activity. Triggers ZBTB4 protein degradation in response to DNA damage. Modulates HMGA1 DNA-binding affinity. In response to high glucose, triggers phosphorylation-mediated subnuclear localization shifting of PDX1. Involved in the regulation of eye size, lens formation and retinal lamination during late embryogenesis.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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