Trpm4 (NM_175130) Mouse Tagged ORF Clone

CAT＃: MR211808

  • TrueORF®

Trpm4 (Myc-DDK-tagged) - Mouse transient receptor potential cation channel, subfamily M, member 4 (Trpm4)

Datasheet
SDS
Plasmid Map

  "NM_175130" in other vectors (3)

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Mouse Protein / Lysate Request

CNY 8,448.00

货期*
现货
规格
    • 10 ug

Product images

经常一起买 (4)
PS100001
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00

添加到购物车
CC100003
DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00

暂停销售
PRIMER
Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

添加到购物车
TA592569S
DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

添加到购物车

【特别福利】购一抗正装产品，免费领10ul抗体试用装

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms 1110030C19Rik; AW047689; LTrpC-4; LTRPC4; TRPM4B
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR211808 representing NM_175130
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGGGGCCGGAGAAGGAGCAGAGCTGGATCCCTAAGATCTTCAGGAAGAAGGTGTGCACGACGTTCA
TAGTGGACCTCTCCGATGATGCGGGAGGGACCTTGTGCCAGTGTGGGCAACCTCGGGATGCCCACCCTTC
TGTGGCTGTGGAGGATGCCTTTGGGGCAGCCGTAGTTACCGAGTGGAACAGTGATGAGCACACCACGGAG
AAGCCCACAGATGCCTATGGGGACCTGGACTTCACGTACTCTGGCCGAAAGCACAGCAACTTTCTCCGGC
TGTCTGACCGCACGGATCCGGCCACTGTGTACAGTCTCGTTACCCGCTCTTGGGGCTTTCGTGCCCCAAA
CTTGGTGGTGTCGGTGCTGGGGGGGTCGGGGGGCCCCGTGCTCCAGACCTGGCTGCAGGACCTTCTGCGT
CGCGGGCTGGTGAGAGCCGCCCAGAGCACAGGGGCCTGGATCGTCACTGGGGGACTGCACACAGGCATTG
GCCGGCACGTGGGTGTGGCTGTAAGGGACCACCAGACAGCCAGCACTGGGAGCAGCAAGGTGGTGGCCAT
GGGTGTGGCCCCCTGGGGAGTGGTCCGGAACAGAGACATGCTGATCAACCCCAAGGGCTCATTCCCCGCA
AGGTACCGGTGGCGTGGTGACCCTGAGGATGGTGTTGAGTTCCCCCTGGACTATAACTATTCTGCTTTCT
TCTTGGTGGATGATGGCACCTATGGCCGCCTGGGTGGTGAGAACCGCTTCCGCCTTCGGTTTGAGTCCTA
TGTGGCTCAGCAGAAGACCGGAGTGGGAGGGACTGGAATTGACATCCCAGTGCTCCTTCTTCTCATCGAT
GGAGATGAGAAGATGTTAAAGCGGATAGAGGATGCCACCCAAGCTCAGCTCCCCTGCCTCTTGGTGGCGG
GCTCTGGGGGTGCTGCAGACTGTCTGGTGGAGACCCTGGAAGATACTCTGGCCCCAGGGAGTGGGGGACT
TAGGCGAGGCGAAGCCCGGGATCGGATTAGGCGATACTTCCCTAAAGGAGACCCTGAGGTTCTTCAGGCC
CAGGTAGAGAGGATCATGACCCGAAAGGAGTTGCTGACGGTCTATTCATCAGAAGACGGCTCCGAGGAGT
TTGAGACTATCGTTTTGAGGGCTCTTGTGAAAGCCTGTGGGAGCTCTGAGGCCTCAGCCTACCTGGATGA
GCTGCGTTTGGCTGTGGCTTGGAACCGCGTGGACATCGCCCAAAGTGAACTTTTCCGTGGGGACATCCAA
TGGCGGTCCTTCCACCTGGAGGCATCTCTCATGGATGCTCTGCTGAATGACCGGCCTGAATTTGTGCGCC
TGCTCATCTCCCATGGCCTTAGCCTGGGACACTTCCTGACCCCCGTGCGTCTGGCCCAGCTGTACAGCGC
GGTGTCCCCTAACTCACTGATCCGAAATCTTCTGGACCAGGCGTCCCATGCTAGTAGCAGCAAATCCCCT
CCTGTAAATGGAACTGTAGAGCTCCGGCCTCCCAATGTGGGGCAAGTGCTGAGGACTCTGTTGGGAGAAA
CGTGTGCACCACGGTACCCTGCCAGGAACACCCGAGACTCCTATCTGGGCCAGGACCACAGAGAGAATGA
CTCTCTGCTTATGGACTGGGCCAACAAGCAACCATCAACGGATGCAAGCTTTGAACAAGCCCCCTGGAGT
GACCTGCTTATTTGGGCTCTGTTGCTGAACCGGGCCCAGATGGCTATCTATTTCTGGGAGAAGGGCTCCA
ACTCAGTGGCCTCTGCTCTTGGGGCTTGTCTTTTACTCCGAGTGATGGCTCGCCTAGAGTCGGAGGCTGA
GGAGGCCGCGCGGCGGAAGGATCTTGCTGCCACGTTTGAAAGCATGAGCGTGGACCTCTTTGGAGAGTGT
TACCACAACAGTGAAGAGCGAGCAGCCCGCCTTCTTCTGCGCCGCTGTCCCCTCTGGGGAGAGGCCACCT
GCCTCCAGCTTGCCATGCAGGCTGATGCCCGAGCGTTCTTTGCCCAGGATGGAGTCCAGTCTCTTCTGAC
ACAGAAGTGGTGGGGGGAGATGGACAGCACAACTCCCATCTGGGCCCTGCTTCTCGCCTTCTTCTGCCCG
CCTCTCATCTACACCAACCTTATCGTCTTCAGGAAGTCAGAGGAGGAGCCCACACAGAAGGATCTTGACT
TTGATATGGACAGCAGCATCAATGGAGCAGGTCCTCCTGGGACTGTAGAACCCTCTGCAAAGGTGGCCCT
TGAGAGGCGGCAGCGGCGACGGCCAGGACGTGCCCTCTGCTGTGGCAAGTTCTCCAAGCGCTGGTCGGAC
TTCTGGGGTGCCCCAGTGACTGCCTTCCTGGGTAATGTGGTCAGTTACCTGCTGTTCCTGCTGCTGTTTG
CCCACGTGTTGCTGGTGGATTTCCAGCCCACAAAGCCAAGTGTCTCCGAGCTGCTGCTGTACTTCTGGGC
CTTCACGTTGCTGTGCGAGGAGCTGCGCCAGGGCCTGGGTGGTGGCTGGGGTAGCCTGGCCAGTGGGGGA
CGTGGTCCGGACAGAGCTCCCCTGCGCCATCGGCTGCACCTCTACCTCTCAGATACCTGGAACCAATGTG
ACCTGCTGGCACTCACCTGCTTCCTGCTGGGCGTAGGCTGCCGGCTGACCCCTGGGCTCTTTGACTTGGG
ACGCACCGTCCTCTGCCTTGACTTCATGATCTTCACACTGCGCCTGCTGCACATCTTCACGGTGAACAAG
CAGCTGGGGCCCAAGATTGTCATAGTGAGCAAAATGATGAAGGATGTATTCTTCTTCCTCTTCTTCCTCT
GCGTGTGGCTTGTAGCTTATGGGGTGGCCACAGAGGGGATCCTGAGGCCCCAGGACCGCAGTCTACCGAG
TATCCTGCGCAGGGTCTTCTACCGACCGTATCTGCAGATCTTCGGACAAATTCCCCAGGAGGAAATGGAC
GTGGCCCTCATGATTCCAGGTAACTGCTCAATGGAGCGGGGCTCCTGGGCTCATCCGGAGGGGCCCGTCG
CAGGCTCCTGTGTGTCCCAGTATGCCAACTGGCTGGTGGTGTTGCTCCTTATCGTCTTCTTGCTGGTGGC
CAATATCCTGTTGCTCAACCTGCTCATCGCCATGTTCAGCTACACCTTCAGCAAAGTGCACGGCAACAGC
GACCTCTACTGGAAGGCACAGCGCTACAGCCTCATCCGGGAATTCCATTCGAGGCCTGCCCTGGCCCCAC
CCCTCATCATCATCTCTCATGTGCGCCTCCTCATCAAGTGGCTGCGCAGGTGTCGCAGGTGTCGCAGGGC
TAACTTGCCCGCCTCCCCAGTCTTCGAGCACTTCCGTGTCTGTCTCTCTAAGGAAGCAGAGCGGAAGCTG
CTGACTTGGGAGTCTGTGCACAAAGAGAACTTCCTGTTGGCACAAGCTCGGGACAAGCGAGACAGTGACT
CGGAGCGTTTGAAACGCACGTCTCAGAAGGTGGACACTGCACTGAAGCAGCTGGGACAGATCAGAGAGTA
CGATCGGCGTCTGAGGGGGCTGGAGAGAGAGGTCCAACACTGTTCTCGAGTCCTGACTTGGATGGCTGAG
GCCCTTAGCCACTCTGCCTTGCTGCCTCCAGGGGCCCCACCCCCTCCGAGCCCCACTGGTTCCAAAGAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
>MR211808 representing NM_175130
Red=Cloning site Green=Tags(s)

MVGPEKEQSWIPKIFRKKVCTTFIVDLSDDAGGTLCQCGQPRDAHPSVAVEDAFGAAVVTEWNSDEHTTE
KPTDAYGDLDFTYSGRKHSNFLRLSDRTDPATVYSLVTRSWGFRAPNLVVSVLGGSGGPVLQTWLQDLLR
RGLVRAAQSTGAWIVTGGLHTGIGRHVGVAVRDHQTASTGSSKVVAMGVAPWGVVRNRDMLINPKGSFPA
RYRWRGDPEDGVEFPLDYNYSAFFLVDDGTYGRLGGENRFRLRFESYVAQQKTGVGGTGIDIPVLLLLID
GDEKMLKRIEDATQAQLPCLLVAGSGGAADCLVETLEDTLAPGSGGLRRGEARDRIRRYFPKGDPEVLQA
QVERIMTRKELLTVYSSEDGSEEFETIVLRALVKACGSSEASAYLDELRLAVAWNRVDIAQSELFRGDIQ
WRSFHLEASLMDALLNDRPEFVRLLISHGLSLGHFLTPVRLAQLYSAVSPNSLIRNLLDQASHASSSKSP
PVNGTVELRPPNVGQVLRTLLGETCAPRYPARNTRDSYLGQDHRENDSLLMDWANKQPSTDASFEQAPWS
DLLIWALLLNRAQMAIYFWEKGSNSVASALGACLLLRVMARLESEAEEAARRKDLAATFESMSVDLFGEC
YHNSEERAARLLLRRCPLWGEATCLQLAMQADARAFFAQDGVQSLLTQKWWGEMDSTTPIWALLLAFFCP
PLIYTNLIVFRKSEEEPTQKDLDFDMDSSINGAGPPGTVEPSAKVALERRQRRRPGRALCCGKFSKRWSD
FWGAPVTAFLGNVVSYLLFLLLFAHVLLVDFQPTKPSVSELLLYFWAFTLLCEELRQGLGGGWGSLASGG
RGPDRAPLRHRLHLYLSDTWNQCDLLALTCFLLGVGCRLTPGLFDLGRTVLCLDFMIFTLRLLHIFTVNK
QLGPKIVIVSKMMKDVFFFLFFLCVWLVAYGVATEGILRPQDRSLPSILRRVFYRPYLQIFGQIPQEEMD
VALMIPGNCSMERGSWAHPEGPVAGSCVSQYANWLVVLLLIVFLLVANILLLNLLIAMFSYTFSKVHGNS
DLYWKAQRYSLIREFHSRPALAPPLIIISHVRLLIKWLRRCRRCRRANLPASPVFEHFRVCLSKEAERKL
LTWESVHKENFLLAQARDKRDSDSERLKRTSQKVDTALKQLGQIREYDRRLRGLEREVQHCSRVLTWMAE
ALSHSALLPPGAPPPPSPTGSKD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_175130
ORF Size 3639 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_175130.4, NP_780339.2
RefSeq Size 4245 bp
RefSeq ORF 3642 bp
Locus ID 68667
UniProt ID Q7TN37
MW 136.2 kDa
Gene Summary Calcium-activated non selective (CAN) cation channel that mediates membrane depolarization. While it is activated by increase in intracellular Ca(2+), it is impermeable to it (PubMed:17188667, PubMed:29211714). Mediates transport of monovalent cations (Na(+) > K(+) > Cs(+) > Li(+)), leading to depolarize the membrane. It thereby plays a central role in cadiomyocytes, neurons from entorhinal cortex, dorsal root and vomeronasal neurons, endocrine pancreas cells, kidney epithelial cells, cochlea hair cells etc. Participates in T-cell activation by modulating Ca(2+) oscillations after T lymphocyte activation, which is required for NFAT-dependent IL2 production. Involved in myogenic constriction of cerebral arteries. Controls insulin secretion in pancreatic beta-cells. May also be involved in pacemaking or could cause irregular electrical activity under conditions of Ca(2+) overload. Affects T-helper 1 (Th1) and T-helper 2 (Th2) cell motility and cytokine production through differential regulation of calcium signaling and NFATC1 localization. Enhances cell proliferation through up-regulation of the beta-catenin signaling pathway (By similarity). Essential for the migration but not the maturation of dendritic cells (PubMed:18758465).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

SKU Description Size Price
MC223963 Trpm4 (untagged) - Mouse transient receptor potential cation channel, subfamily M, member 4 (Trpm4), (10ug)
    • 10 ug

CNY 15,580.00

MR211808L3 Lenti ORF clone of Trpm4 (Myc-DDK-tagged) - Mouse transient receptor potential cation channel, subfamily M, member 4 (Trpm4)
    • 10 ug

CNY 11,500.00

MR211808L4 Lenti ORF clone of Trpm4 (mGFP-tagged) - Mouse transient receptor potential cation channel, subfamily M, member 4 (Trpm4)
    • 10 ug

CNY 11,500.00

You may also need
plasmid icon Empty Vector
protein iconDDK/Flag Antibody

其它Trpm4产品

Expression Plasmids
Lentiviral Particles
CRISPR
RNAi
Proteins
Antibodies
相关产品 (8)

PS100001

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【特别福利】购一抗正装产品，免费领10ul抗体试用装

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MC223963

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MR211808L4

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MR211808L3V

Lenti ORF particles, Trpm4 (Myc-DDK-tagged) - Mouse transient receptor potential cation channel, subfamily M, member 4 (Trpm4), 200ul, >10^7 TU/mL

    • 200 ul

CNY 17,670.00 暂停销售

MR211808L4V

Lenti ORF particles, Trpm4 (GFP-tagged) - Mouse transient receptor potential cation channel, subfamily M, member 4 (Trpm4), 200ul, >10^7 TU/mL

    • 200 ul

CNY 19,570.00 暂停销售

MR211808L3

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    • 10 ug

CNY 11,500.00

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