Tpp2 (BC058239) Mouse Tagged ORF Clone
CAT#: MR211854
- TrueORF®
Tpp2 (Myc-DDK-tagged) - Mouse tripeptidyl peptidase II (cDNA clone MGC:65300 IMAGE:6308147)
ORF Plasmid: tGFP
"BC058239" in other vectors (4)
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CNY 8,696.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | TPP-2; TppII |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR211854 representing BC058239
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCACCGCCGCGACCGAGGAGCCCTTTCCTTTCCACGGTCTTCTACCAAAGAAGGAGACCGGGGCCT CCTCCTTCCTGTGCCGCTACCCGGAGTATGACGGGCGCGGGGTGCTCATCGCCGTCCTGGACACAGGGGT CGATCCCGGGGCCCCGGGCATGCAGGTTACAACTGATGGAAAACCAAAAATCATTGATATCATTGATACA ACAGGAAGTGGTGATGTAAATACTGCTACAGAAGTAGAACCAAAAGATGGTGAAATTATTGGTCTTTCTG GAAGAGTGCTTAAGATTCCTGCAAACTGGACAAATCCTTTAGGAAAATATCATATTGGCATTAAAAATGG TTATGACTTCTATCCAAAGGCTCTCAAGGAAAGGATACAGAAAGAACGGAAGGAAAAAATCTGGGATCCA ATTCACAGAGTTGCACTTGCAGAAGCTTGTAGAAAACAAGAAGAATTTGATATTGCCAACAATGGCTCTT CCCAAGCCAATAAACTAATCAAGGAAGAGTTGCAAAGTCAAGTGGAATTACTTAATTCTTTTGAGAAAAA GTATAGTGATCCAGGCCCTGTATATGACTGCTTGGTGTGGCATGATGGTGAGACCTGGAGAGCCTGTGTT GATTCAAATGAGAATGGGGACTTGAGTAAATGTGCCGTATTGAGAAACTACAAAGAGGCCCAAGAGTACA GTTCTTTTGGCACAGCTGAGATGCTGAATTACTCTGTGAACATTTATGACGATGGGAACCTGCTCTCCAT TGTGACCAGCGGAGGAGCTCATGGAACCCATGTAGCAAGTATAGCCGCAGGGCATTTTCCAGAAGAGCCT GAACGGAATGGAGTTGCTCCTGGTGCTCAAATTCTATCCATTAAGATTGGTGATACACGGCTAAGCACTA TGGAAACAGGCACAGGCCTCATCAGAGCTATGATAGAAGTTATAAATCATAAGTGTGATCTTGTCAACTA CAGTTATGGAGAAGCAACTCATTGGCCAAATTCTGGGAGAATTTGTGAAGTAATTAATGAAGCAGTATGG AAACATAATACAATTTATGTTTCAAGTGCTGGAAATAATGGTCCATGCCTTTCTACAGTGGGTTGTCCAG GAGGAACTACATCCAGTGTGATAGGTGTTGGAGCTTATGTTTCCCCTGATATGATGGTTGCAGAGTATTC ACTGAGAGAGAAACTGCCTGCAAATCAATATACATGGTCTTCTAGAGGCCCAAGTGCTGATGGAGCCCTC GGTGTGAGCATCAGTGCACCAGGAGGTGCTATTGCTTCTGTGCCTAACTGGACATTGAGGGGGACTCAGC TAATGAATGGGACATCAATGTCTTCCCCCAATGCCTGTGGTGGCATTGCCCTGGTACTTTCAGGGCTGAA AGCAAATAATGTTGACTATACTGTACACTCAGTCAGAAGAGCTCTAGAAAATACTGCAATAAAAGCTGAC AATATAGAAGTATTTGCCCAAGGACATGGAATTATTCAGGTTGACAAAGCTTATGACTACCTCATTCAAA ATACATCATTTGCTAACAGATTAGGTTTTACAGTTACTGTTGGAAATAACCGTGGTATCTACCTCCGAGA TCCTGTCCAGGTGGCTGCTCCTTCAGATCATGGTGTTGGCATTGAGCCTGTATTTCCAGAGAACACAGAA AACTCTGAAAAAATATCCTTTCAGCTTCATTTAGCTTTAACTTCAAATTCATCTTGGGTTCAGTGTCCCA GCCATTTGGAACTCATGAATCAGTGTCGGCACATAAACATACGTGTGGACCCCAGGGGCTTAAGAGAAGG GTTACATTATACAGAGGTATGTGGCTATGATATAGCATCCCCCAATGCAGGTCCTTTATTCAGAGTTCCA ATCACTGCAGTTATAGCAGCAAAAGTAAATGAGTCATCACATTATGATCTAGCCTTTACAGATGTACATT TTAAACCTGGTCAGATTCGAAGACATTTTGTTGAGGTTCCTGAAGGGGCAACCTGGGCTGAAGTTACCGT GTGTTCATGTTCTTCTGAGGTATCGGCAAAATTTGTTCTTCATGCAGTACAGCTTGTGAAGCAGAGAGCA TATCGAAGTCATGAATTTTATAAGTTTTGTTCCCTTCCAGAAAAAGGAACACTTATTGAAGCTTTTCCTG TTTTGGGCGGAAAAGCAATTGAATTTTGTATTGCTCGTTGGTGGGCAAGTCTTAGTGATGTCAATATTGA TTATACCATATCATTCCATGGGATAGTGTGTACTGCACCACAGTTAAACATTCATGCATCTGAAGGAATC AATCGTTTTGATGTTCAGTCCTCTTTAAAGTATGAAGATCTGGCTCCTTGCATAACTTTGAAGAGCTGGG TGCAAACGCTACGCCCAGTAAATGCAAAAACCAGACCTTTAGGATCAAGAGATGTTTTGCCAAATAATCG CCAGCTTTATGAGATGGTCCTTACATACAGCTTTCATCAGCCCAAGAGCGGAGAAGTAACACCTAGTTGT CCACTCCTTTGTGAATTGTTATATGAGTCAGAATTTGACAGTCAGTTGTGGATTATTTTCGACCAGAACA AAAGACAGATGGGCTCAGGCGATGCCTATCCACATCAGTATTCTCTGAAATTGGAGAAAGGAGATTATAC AATTCGATTACAGATTCGTCATGAGCAAATCAGTGATTTGGATCGTCTCAAAGATCTTCCGTTTATTGTT TCACATAGGTTGTCTAATACCTTGAGCTTAGATATTCATGAAAATCATAGCCTTGCACTTCTAGGAAAGA AGAAATCAAGCAGCTTAACATTACCACCCAAATACAATCAGCCATTCTTTGTTACTTCCTTACCTGATGA TAAAATACCTAAGGGGGCAGGACCCGGATGCTACCTTGCAGGCTCCTTGACATTGTCAAAGACTGAGCTT GGAAAGAAAGCTGATGTGATCCCTGTTCATTACTATCTCATACCTCCACCAACAAAGATTAAGAATGGCA GCAAAGATAAAGAAAAGGATTCAGAAAAAGAGAAAGACTTGAAAGAAGAGTTTACTGAAGCATTACGCGA TCTCAAAATTCAGTGGATGACCAAGCTTGATTCTACTGACATTTACAATGAATTGAAAGAAACATATCCT GCTTACCTTCCTTTGTATGTTGCACGTCTTCATCAACTGGATGCTGAAAAGGAACGAATGAAAAGACTTA ATGAAATTGTTGATGCTGCCAATGCTGTTATTTCTCACATCGATCAAACCGCTCTTGCAGTTTACATTGC AATGAAGACTGACCCCAGGCCTGATGCAGCTACTATAAAAAATGATATGGACAAGCAGAAATCCACCCTG ATAGATGCCCTCTGCAGGAAAGGATGTGCCCTGGCAGATCACCTTCTTCATACACAGCCTCACGACGGGG CAGCAGCTGGAGATGCTGAAGCAAAGGAAGAAGAAGGAGAAAGTACCATGGAATCTCTATCGGAAACCTA TTGGGAAACTACAAAGTGGACAGATCTTTTTGACACTAAGGTTTTGATATTTGCATACAAGCATGCATTA GTAAATAAGATGTATGGGAGAGGCCTTAAGTTTGCAACCAAACTCGTAGAAGAAAAACCAACAAAAGAAA ACTGGAAAAATTGTATTCAACTCATGAAATTACTTGGATGGACCCACTGTGCATCTTTTACTGAAAACTG GCTCCCCATCATGTATCCTCCTGATTATTGTGTATTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211854 representing BC058239
Red=Cloning site Green=Tags(s) MATAATEEPFPFHGLLPKKETGASSFLCRYPEYDGRGVLIAVLDTGVDPGAPGMQVTTDGKPKIIDIIDT TGSGDVNTATEVEPKDGEIIGLSGRVLKIPANWTNPLGKYHIGIKNGYDFYPKALKERIQKERKEKIWDP IHRVALAEACRKQEEFDIANNGSSQANKLIKEELQSQVELLNSFEKKYSDPGPVYDCLVWHDGETWRACV DSNENGDLSKCAVLRNYKEAQEYSSFGTAEMLNYSVNIYDDGNLLSIVTSGGAHGTHVASIAAGHFPEEP ERNGVAPGAQILSIKIGDTRLSTMETGTGLIRAMIEVINHKCDLVNYSYGEATHWPNSGRICEVINEAVW KHNTIYVSSAGNNGPCLSTVGCPGGTTSSVIGVGAYVSPDMMVAEYSLREKLPANQYTWSSRGPSADGAL GVSISAPGGAIASVPNWTLRGTQLMNGTSMSSPNACGGIALVLSGLKANNVDYTVHSVRRALENTAIKAD NIEVFAQGHGIIQVDKAYDYLIQNTSFANRLGFTVTVGNNRGIYLRDPVQVAAPSDHGVGIEPVFPENTE NSEKISFQLHLALTSNSSWVQCPSHLELMNQCRHINIRVDPRGLREGLHYTEVCGYDIASPNAGPLFRVP ITAVIAAKVNESSHYDLAFTDVHFKPGQIRRHFVEVPEGATWAEVTVCSCSSEVSAKFVLHAVQLVKQRA YRSHEFYKFCSLPEKGTLIEAFPVLGGKAIEFCIARWWASLSDVNIDYTISFHGIVCTAPQLNIHASEGI NRFDVQSSLKYEDLAPCITLKSWVQTLRPVNAKTRPLGSRDVLPNNRQLYEMVLTYSFHQPKSGEVTPSC PLLCELLYESEFDSQLWIIFDQNKRQMGSGDAYPHQYSLKLEKGDYTIRLQIRHEQISDLDRLKDLPFIV SHRLSNTLSLDIHENHSLALLGKKKSSSLTLPPKYNQPFFVTSLPDDKIPKGAGPGCYLAGSLTLSKTEL GKKADVIPVHYYLIPPPTKIKNGSKDKEKDSEKEKDLKEEFTEALRDLKIQWMTKLDSTDIYNELKETYP AYLPLYVARLHQLDAEKERMKRLNEIVDAANAVISHIDQTALAVYIAMKTDPRPDAATIKNDMDKQKSTL IDALCRKGCALADHLLHTQPHDGAAAGDAEAKEEEGESTMESLSETYWETTKWTDLFDTKVLIFAYKHAL VNKMYGRGLKFATKLVEEKPTKENWKNCIQLMKLLGWTHCASFTENWLPIMYPPDYCVF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | BC058239 |
ORF Size | 3747 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | BC058239.1 |
RefSeq Size | 3960 bp |
RefSeq ORF | 3749 bp |
Locus ID | 22019 |
MW | 145.2 kDa |
Gene Summary | Component of the proteolytic cascade acting downstream of the 26S proteasome in the ubiquitin-proteasome pathway. May be able to complement the 26S proteasome function to some extent under conditions in which the latter is inhibited (By similarity). Stimulates adipogenesis.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC223079 | Tpp2 (untagged) - Mouse tripeptidyl peptidase II (cDNA clone MGC:65300 IMAGE:6308147), (10ug) |
CNY 12,920.00 |
|
MG211854 | Tpp2 (tGFP-tagged) - Mouse tripeptidyl peptidase II (cDNA clone MGC:65300 IMAGE:6308147) |
CNY 7,890.00 |
|
MR211854L3 | Lenti ORF clone of Tpp2 (Myc-DDK-tagged) - Mouse tripeptidyl peptidase II (cDNA clone MGC:65300 IMAGE:6308147) |
CNY 9,030.00 |
|
MR211854L4 | Lenti ORF clone of Tpp2 (mGFP-tagged) - Mouse tripeptidyl peptidase II (cDNA clone MGC:65300 IMAGE:6308147) |
CNY 9,030.00 |