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Sympk (BC049852) Mouse Tagged ORF Clone
CAT＃: MR211888
- TrueORF®
Sympk (Myc-DDK-tagged) - Mouse symplekin (cDNA clone MGC:54793 IMAGE:4163760)
ORF Plasmid: tGFP
"BC049852" in other vectors (4)
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CNY 8,944.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | SPK, SYM |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211888 representing BC049852
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAAGCAGCAGCGGGGACAGTGTCACCCGCCGGAGTGTGGCATCACAGTTTTTCACACAAGAAGAAG GGCCAAGCATCGATGGCATGACCACTTCTGAGAGGGTGGTAGATCTTCTGAACCAGGCTGCATTGATCAC CAATGACTCCAAGATCACAGTGCTCAAACAGGTCCAAGAGCTGATCATCAACAAGGACCCCACCCTGCTT GACAACTTCCTGGATGAGATCATCGCTTTCCAAGCAGACAAATCCATTGAAGTGCGCAAGTTTGTCATTG GCTTCATTGAGGAGGCATGCAAGCGGGACATTGAGCTGCTGCTAAAACTGATTGCCAACCTCAACATGCT ATTGCGGGATGAAAATGTGAACGTGGTTAAGAAGGCCATCCTCACCATGACCCAGCTCTACAAGGTGGCC CTACAGTGGATGGTGAAGTCTCGAGTCATCAGTGATCTCCAGGAGGCCTGCTGGGACATGGTGTCCTCCA TGGCTGGGGAAATCATCCTGCTTTTGGATTCTGACAACGATGGCATCCGTACACATGCCATCAAGTTTGT GGAGGGCCTCATTGTCACCCTGTCACCCCGCATGGCTGACTCAGAGGTCCCACGACGCCAGGAGCATGAC ATCAGTCTGGACCGCATTCCTCGAGATCACCCCTACATCCAGTACAATGTCCTATGGGAAGAAGGCAAGG CAGCTGTGGAGCAGCTGCTGAAGTTCATGGTGCACCCCGCCATCTCCTCCATCAACCTGACCACAGCGCT CGGCTCCCTTGCCAACATCGCCCGCCAGAGACCCATGTTCATGTCCGAGGTGATCCAGGCCTATGAGACT CTGCATGCCAACCTGCCCCCAACGCTGGCCAAGTCCCAGGTGAGCAGTGTGCGGAAGAACCTTAAGCTGC ACTTGCTCAGTGTGCTCAAGCACCCTGCCTCCTTGGAGTTCCAGGCCCAGATCACCACCCTGCTGGTGGA TCTGGGCACGCCCCAGGCTGAGATCGCCCGAAACATGCCTAGCAGCAAGGACTCCCGCAAGCGGCCCCGG GACGATACAGATTCCACGCTCAAGAAGATGAAGCTGGAGCCCAACTTGGGAGAAGACGATGAGGACAAAG ACTTGGAGCCTGGCCCATCAGGGACGTCAAAGGCGTCAGCCCAGATCTCAGGCCAGTCAGACACGGACAT CACTGCTGAGTTCTTGCAGCCTCTGCTGACACCTGACAATGTGGCCAATCTGGTCCTCATCAGCATGGTG TACCTGCCTGAGACCATGCCTGCCTCCTTCCAAGCCATCTACACCCCTGTGGAGTCAGCAGGCACTGAAG CCCAGATCAAGCACCTGGCTCGACTCATGGCCACACAGATGACAGCTGCAGGACTGGGTCCTGGTGTGGA ACAGACGAAACAGTGCAAGGAGGAGCCCAAGGAGGAGAAGGTGGTAAAGCCGGAGAGCGTCCTGATCAAG CGGCGCCTGTCGGTGCAGGGCCAGGCCATCTCTGTGGTAGGCTCTCAGAGCACCATGTCTCCCTTGGAGG AGGAGGTACCCCAGGCCAAGAGGAGGCCAGAACCCATCATCCCTGTCACGCAGCCACGGCTGGCAGGCGC AGGTGGGCGCAAGAAGATCTTCCGTCTGAGCGATGTGCTGAAGCCCCTGACAGATGCCCAGGTGGAAGCC ATGAAGCTAGGAGCTGTGAAGCGGATCCTGAGGGCTGAGAAGGCTGTAGCCTGCAGTGGGGCAGCCCAGG TGCGCATCAAGATCCTGGCCAGCCTCGTAACACAGTTCGACTCCGGATTCAAGGCAGAGGTGCTGTCCTT TATCCTGGAAGATGTCCGGGCCCGTCTGGACCTGGCTTTTGCCTGGCTCTACCAGGAATATAATGCCTAC CTGGCAGCAGGGACCTCAGGCACTCTGGACAAATATGAGGATTGCCTCATCTGCCTGCTCTCTGGACTAC AAGAGAAACCAGACCAGAAAGATGGGATCTTTACCAAGGTGGTTTTGGAGGCCCCACTGATCACTGAGAG TGCCTTGGAGGTGATACGGAAGTACTGTGAGGATGAGAGTCGTGCCTACCTGGGCATGTCGACACTTGGA GACCTGATCTTCAAGCGCCCTTCTCGCCAGTTCCAGTATCTGCACGTTCTTCTTGACCTCAGCTCTCACG AGAAGGACAGGGTGCGCTCCCAGGCCTTGCTCTTCATTAAGCGAATGTATGAGAAGGAGCAGCTTCGAGA GTATGTGGAGAAATTTGCCCTCAACTACCTGCAGCTCCTGGTCCACCCCAACCCACCCTCGGTGCTGTTT GGAGCCGACAAGGACACAGAGGTGGCCGCACCCTGGACAGAGGAAACAGTGAAGCAGTGTTTGTATCTGT ATCTGGCTCTCCTGCCTCAGAACCACAAGCTGATCCATGAGCTGGCAGCCGTGTACACTGAAGCCATCGC TGACATCAAGCGGACAGTGCTGAGGGTCATTGAGCAGCCGATCCGAGGGATGGGCATGAACTCTCCAGAG CTGCTGCTGCTGGTAGAAAACTGTCCCAAGGGGGCAGAGACGCTGGTCACCCGCTGCCTGCACAGCCTCA CCGACAAAGTGCCACCATCCCCAGAACTGGTGAAGCGGGTCCGAGACCTGTACCACAAACGGCTGCCTGA TGTGCGCTTCCTCATCCCCGTGCTCAATGGGTTGGAGAAGAAAGAGGTGATCCAGGCGCTGCCCAAGCTC ATCAAACTCAACCCCATTGTGGTGAAGGAAGTCTTCAACCGCCTGCTAGGCACTCAGCACGGTGAGGGGA ACTCGGCCCTGTCCCCCCTGAATCCAGGAGAGCTGCTGATTGCATTGCACAATATTGACTCTGTGAAGTG TGACATGAAGTCCATCATCAAAGCCACCAACCTGTGTTTTGCGGAGCGGAATGTGTACACGTCGGAAGTG CTAGCTGTGGTGATGCAGCAGCTGATGGAGCAAAGCCCTCTGCCCATGCTGCTCATGCGGACCGTCATCC AGTCCCTCACCATGTACCCCCGCCTGGGGGGCTTCGTCATGAACATCCTGGCCCGACTCATCATGAAGCA GGTATGGAAGTACCCCAAGGTATGGGAGGGCTTCATCAAGTGCTGCCAGCGCACCAAGCCACAGAGTTTC CAGGTCATCCTGCAGCTACCTCCACAGCAGCTTGGCGCAGTCTTTGACAAGTGCCCTGAGCTCCGGGAGC CACTGTTGGCCCATGTGCGCTCCTTCACTCCCCACCAGCAAGCACACATCCCCAACTCCATCATGACCAT CCTGGAGGCCACTGGAAAGCAGGAGCCCGAAGTCAAGGAAGCACCTTCAGGGCCCCTGGAAGAGGATGAC CTGGAGCCCTTGGCTCTGGCTTTGGCCCCGGCTCCGGCCCCGGCCCCGGCCCCGGCCCCTGCCCCACGGC CCCCTCAGGACCTCATCGGCCTGCGATTGGCTCAGGAGAAGGCCCTGAAGAGGCAGCTGGAGGAGGAACA GAAGCAGAAGCCCACAGGCATCGGAGCGCCCGCTGCCTGTGTGTCCTCAACGCCCTCGGTGCCAGCAGCA GCCCGAGCTGGGCCCACCCCAGCTGAGGAGGTCATGGAATACCGGGAGGAGGGTCCCGAATGCGAGACCC CGGCCATCTTCATCAGCATGGACGACGACTCGGGGCTGGCTGAAACCACACTGCTGGACTCTAGTCTCGA GGGGCCCCTGCCTAAGGAGGCAGCAGCAGTCGGGTCGAGCTCCAAGGATGAGCGGAGCCCCCAGAACCTC AGCCATGCTGTGGAAGAAGCCTTGAAGACCTCCAGCCCAGAAACCAGGGAACCCGAGAGCAAGGGGAACA GC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211888 representing BC049852
Red=Cloning site Green=Tags(s) MASSSGDSVTRRSVASQFFTQEEGPSIDGMTTSERVVDLLNQAALITNDSKITVLKQVQELIINKDPTLL DNFLDEIIAFQADKSIEVRKFVIGFIEEACKRDIELLLKLIANLNMLLRDENVNVVKKAILTMTQLYKVA LQWMVKSRVISDLQEACWDMVSSMAGEIILLLDSDNDGIRTHAIKFVEGLIVTLSPRMADSEVPRRQEHD ISLDRIPRDHPYIQYNVLWEEGKAAVEQLLKFMVHPAISSINLTTALGSLANIARQRPMFMSEVIQAYET LHANLPPTLAKSQVSSVRKNLKLHLLSVLKHPASLEFQAQITTLLVDLGTPQAEIARNMPSSKDSRKRPR DDTDSTLKKMKLEPNLGEDDEDKDLEPGPSGTSKASAQISGQSDTDITAEFLQPLLTPDNVANLVLISMV YLPETMPASFQAIYTPVESAGTEAQIKHLARLMATQMTAAGLGPGVEQTKQCKEEPKEEKVVKPESVLIK RRLSVQGQAISVVGSQSTMSPLEEEVPQAKRRPEPIIPVTQPRLAGAGGRKKIFRLSDVLKPLTDAQVEA MKLGAVKRILRAEKAVACSGAAQVRIKILASLVTQFDSGFKAEVLSFILEDVRARLDLAFAWLYQEYNAY LAAGTSGTLDKYEDCLICLLSGLQEKPDQKDGIFTKVVLEAPLITESALEVIRKYCEDESRAYLGMSTLG DLIFKRPSRQFQYLHVLLDLSSHEKDRVRSQALLFIKRMYEKEQLREYVEKFALNYLQLLVHPNPPSVLF GADKDTEVAAPWTEETVKQCLYLYLALLPQNHKLIHELAAVYTEAIADIKRTVLRVIEQPIRGMGMNSPE LLLLVENCPKGAETLVTRCLHSLTDKVPPSPELVKRVRDLYHKRLPDVRFLIPVLNGLEKKEVIQALPKL IKLNPIVVKEVFNRLLGTQHGEGNSALSPLNPGELLIALHNIDSVKCDMKSIIKATNLCFAERNVYTSEV LAVVMQQLMEQSPLPMLLMRTVIQSLTMYPRLGGFVMNILARLIMKQVWKYPKVWEGFIKCCQRTKPQSF QVILQLPPQQLGAVFDKCPELREPLLAHVRSFTPHQQAHIPNSIMTILEATGKQEPEVKEAPSGPLEEDD LEPLALALAPAPAPAPAPAPAPRPPQDLIGLRLAQEKALKRQLEEEQKQKPTGIGAPAACVSSTPSVPAA ARAGPTPAEEVMEYREEGPECETPAIFISMDDDSGLAETTLLDSSLEGPLPKEAAAVGSSSKDERSPQNL SHAVEEALKTSSPETREPESKGNS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC049852 |
| ORF Size | 3852 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC049852.1 |
| RefSeq Size | 4071 bp |
| RefSeq ORF | 3854 bp |
| Locus ID | 68188 |
| MW | 149.3 kDa |
| Gene Summary | Scaffold protein that functions as a component of a multimolecular complex involved in histone mRNA 3'-end processing. Specific component of the tight junction (TJ) plaque, but might not be an exclusively junctional component. May have a house-keeping rule. Is involved in pre-mRNA polyadenylation. Enhances SSU72 phosphatase activity (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223093 | Sympk (untagged) - Mouse symplekin (cDNA clone MGC:54793 IMAGE:4163760), (10ug) |
CNY 12,920.00 |
|
| MG211888 | Sympk (tGFP-tagged) - Mouse symplekin (cDNA clone MGC:54793 IMAGE:4163760) |
CNY 8,080.00 |
|
| MR211888L3 | Lenti ORF clone of Sympk (Myc-DDK-tagged) - Mouse symplekin (cDNA clone MGC:54793 IMAGE:4163760) |
CNY 9,220.00 |
|
| MR211888L4 | Lenti ORF clone of Sympk (mGFP-tagged) - Mouse symplekin (cDNA clone MGC:54793 IMAGE:4163760) |
CNY 9,220.00 |
