Col18a1 (NM_009929) Mouse Tagged ORF Clone
CAT#: MR211918
- TrueORF®
Col18a1 (Myc-DDK-tagged) - Mouse collagen, type XVIII, alpha 1 (Col18a1), transcript variant 2
ORF Plasmid: tGFP
"NM_009929" in other vectors (2)
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CNY 13,110.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | endostatin |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR211918 representing NM_009929
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCCCAGGTGGCACCTCCTGGATGTGCTCACCAGTTTGGTCTTGCTGCTGGTGGCACGCGTCTCCT GGGCAGAGCCAGAGAATGTTGCTGAGGAGGTGGGGCTGCTGCAGCTCCTTGGAGACCCCCTACCTGAGAA GATCTCACAAATCGATGACCCTCACGTCGGGCCGGCCTACATCTTTGGACCAGACTCCAACAGTGGCCAG GTGGCCCAGTATCATTTCCCAAAACTCTTCTTCCGGGACTTTTCGCTGCTGTTTCATGTCCGGCCAGCCA CAGAGGCAGCAGGGGTGCTATTTGCCATCACAGATGCTGCCCAGGTGGTAGTCTCACTGGGCGTGAAGCT CTCAGAGGTCCGAGATGGACAGCAAAACATCTCATTGCTCTACACGGAGCCTGGGGCCAGCCAGACCCAG ACGGGAGCCAGCTTCCGCCTACCTGCATTTGTTGGGCAGTGGACACACTTCGCGCTCAGCGTCGACGGAG GCTCTGTGGCTCTCTACGTAGACTGTGAAGAATTCCAGAGGGTGCCATTTGCTCGGGCCTCGCAGGGACT GGAGCTAGAGCGTGGCGCTGGCCTCTTTGTGGGTCAGGCTGGAACAGCAGACCCTGACAAGTTCCAGGGG ATGATCTCAGAGCTGAAGGTACGCAAAACCCCCCGGGTGAGCCCTGTGCACTGTCTGGATGAAGAAGATG ATGATGAAGACCGGGCATCTGGAGATTTTGGAAGTGGCTTTGAAGAAAGCAGCAAGTCACACAAGGAGGA TACATCTCTACTACCTGGGCTCCCTCAGCCACCTCCTGTCACTTCCCCACCCCTGGCTGGAGGCAGCACC ACAGAAGATCCTAGAACAGAAGAAACGGAGGAAGACGCCGCGGTAGATTCTATAGGAGCTGAGACCCTTC CTGGCACAGGTTCAAGCGGTGCATGGGATGAGGCTATCCAGAACCCCGGAAGGGGCTTGATAAAGGGAGG TATGAAAGGACAAAAGGGAGAACCAGGTGCCCAGGGCCCACCTGGCCCAGCTGGGCCCCAGGGTCCTGCC GGTCCAGTGGTCCAGAGCCCCAACTCACAACCTGTCCCTGGAGCACAAGGACCCCCGGGACCTCAGGGGC CACCAGGGAAGGATGGCACTCCAGGAAGGGATGGTGAACCGGGTGACCCTGGTGAAGATGGGAGACCGGG TGACACTGGACCTCAAGGCTTTCCAGGGACCCCAGGAGATGTGGGCCCTAAGGGCGAGAAGGGAGATCCT GGTATTGGGCCCCGAGGACCTCCAGGGCCTCCAGGGCCACCAGGACCCTCCTTCAGACAAGACAAGCTGA CCTTCATTGACATGGAGGGATCCGGTTTCAGCGGAGACATAGAGAGCCTTAGAGGCCCACGAGGCTTCCC TGGCCCCCCGGGGCCCCCTGGTGTCCCAGGACTTCCTGGTGAGCCAGGACGCTTTGGGATCAATGGTTCC TATGCACCAGGACCTGCAGGCCTTCCTGGTGTACCTGGGAAGGAAGGACCCCCCGGTTTTCCAGGTCCCC CGGGACCTCCAGGTCCTCCAGGCAAAGAGGGCCCACCAGGAGTGGCCGGCCAGAAAGGCAGTGTTGGTGA TGTGGGCATCCCAGGACCCAAGGGGAGCAAAGGAGACCTTGGGCCCATCGGTATGCCTGGCAAGTCTGGC TTGGCTGGATCCCCTGGGCCAGTTGGACCCCCAGGACCTCCAGGGCCTCCAGGGCCACCAGGACCAGGAT TTGCTGCTGGATTCGATGATATGGAAGGCTCTGGAATACCCCTCTGGACAACAGCCCGAAGCTCTGATGG GCTGCAGGGACCTCCCGGGTCGCCGGGACTCAAGGGGGATCCTGGAGTGGCAGGCCTACCTGGAGCCAAG GGAGAAGTTGGAGCAGATGGAGCCCAGGGCATCCCTGGTCCCCCAGGAAGAGAAGGTGCAGCTGGATCTC CGGGGCCAAAAGGAGAGAAAGGGATGCCGGGAGAAAAGGGAAACCCAGGAAAAGATGGAGTGGGCCGGCC GGGCCTCCCTGGGCCTCCAGGACCTCCAGGGCCTGTGATCTATGTGTCAAGTGAGGATAAAGCAATAGTG AGCACGCCAGGACCTGAGGGCAAGCCAGGGTACGCAGGCTTTCCTGGACCTGCTGGACCGAAGGGTGACC TGGGTTCCAAAGGCGAGCAGGGTCTTCCGGGGCCCAAGGGTGAGAAGGGAGAGCCAGGCACTATCTTTAG TCCTGATGGCAGAGCTCTGGGCCATCCCCAGAAGGGAGCCAAGGGAGAGCCAGGCTTTCGAGGACCCCCG GGTCCTTATGGACGACCTGGGCACAAGGGTGAAATTGGCTTCCCTGGACGGCCGGGTCGACCTGGAACGA ATGGCTTAAAGGGAGAGAAAGGAGAGCCTGGAGATGCCAGCCTTGGGTTCAGCATGAGGGGATTGCCTGG CCCCCCTGGGCCTCCAGGACCCCCAGGTCCTCCTGGGATGCCCATCTATGACAGCAATGCATTTGTGGAG TCTGGCCGACCTGGACTACCAGGACAGCAGGGTGTGCAGGGGCCTTCAGGACCAAAGGGTGACAAAGGAG AGGTGGGCCCACCTGGGCCACCAGGGCAATTCCCCATTGACCTCTTCCACCTGGAAGCGGAAATGAAGGG GGACAAGGGAGACCGAGGGGATGCTGGACAGAAAGGAGAGAGGGGAGAACCTGGGGCTCCTGGTGGTGGA TTCTTCAGCTCAAGTGTACCTGGCCCCCCCGGCCCACCTGGATACCCTGGAATTCCGGGTCCAAAGGGAG AGAGCATCCGGGGGCCACCTGGCCCTCCTGGCCCGCAGGGACCTCCTGGCATTGGCTATGAGGGTCGCCA GGGTCCCCCAGGACCTCCGGGACCTCCAGGACCTCCCTCCTTCCCTGGCCCTCACAGACAGACTGTCAGT GTTCCTGGTCCTCCGGGCCCACCTGGTCCTCCAGGTCCCCCAGGAGCCATGGGTGCCTCTGCTGGGCAGG TGAGGATCTGGGCCACATACCAGACCATGCTGGACAAGATCCGGGAGGTGCCGGAGGGCTGGCTCATCTT TGTGGCCGAGAGGGAAGAGCTCTATGTACGCGTTAGAAATGGCTTCCGGAAGGTGCTGCTGGAGGCCCGG ACAGCCCTCCCGAGAGGCACGGGCAATGAGGTGGCTGCTTTGCAGCCCCCATTGGTCCAGCTTCATGAGG GCAGTCCATACACCCGGAGGGAGTACTCCTATTCCACGGCACGACCCTGGCGAGCAGATGACATCCTGGC CAACCCACCGCGCCTGCCAGACCGCCAGCCTTACCCCGGAGTTCCACATCACCACAGTTCCTATGTGCAC CTGCCGCCAGCCCGCCCCACCCTCTCACTTGCTCATACTCATCAGGACTTTCAGCCAGTGCTCCACCTGG TGGCACTGAACACCCCCCTGTCTGGAGGCATGCGTGGTATCCGTGGAGCAGATTTCCAGTGCTTCCAGCA AGCCCGAGCCGTGGGGCTGTCGGGCACCTTCCGGGCTTTCCTGTCCTCTAGGCTGCAGGATCTCTATAGC ATCGTGCGCCGTGCTGACCGGGGGTCTGTGCCCATCGTCAACCTGAAGGACGAGGTGCTATCTCCCAGCT GGGACTCCCTGTTTTCTGGCTCCCAGGGTCAACTGCAACCCGGGGCCCGCATCTTTTCTTTTGACGGCAG AGATGTCCTGAGACACCCAGCCTGGCCGCAGAAGAGCGTATGGCACGGCTCGGACCCCAGTGGGCGGAGG CTGATGGAGAGTTACTGTGAGACATGGCGAACTGAAACTACTGGGGCTACAGGTCAGGCCTCCTCCCTGC TGTCAGGCAGGCTCCTGGAACAGAAAGCTGCGAGCTGCCACAACAGCTACATCGTCCTGTGCATTGAGAA TAGCTTCATGACCTCTTTCTCCAAA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >MR211918 representing NM_009929
Red=Cloning site Green=Tags(s) MAPRWHLLDVLTSLVLLLVARVSWAEPENVAEEVGLLQLLGDPLPEKISQIDDPHVGPAYIFGPDSNSGQ VAQYHFPKLFFRDFSLLFHVRPATEAAGVLFAITDAAQVVVSLGVKLSEVRDGQQNISLLYTEPGASQTQ TGASFRLPAFVGQWTHFALSVDGGSVALYVDCEEFQRVPFARASQGLELERGAGLFVGQAGTADPDKFQG MISELKVRKTPRVSPVHCLDEEDDDEDRASGDFGSGFEESSKSHKEDTSLLPGLPQPPPVTSPPLAGGST TEDPRTEETEEDAAVDSIGAETLPGTGSSGAWDEAIQNPGRGLIKGGMKGQKGEPGAQGPPGPAGPQGPA GPVVQSPNSQPVPGAQGPPGPQGPPGKDGTPGRDGEPGDPGEDGRPGDTGPQGFPGTPGDVGPKGEKGDP GIGPRGPPGPPGPPGPSFRQDKLTFIDMEGSGFSGDIESLRGPRGFPGPPGPPGVPGLPGEPGRFGINGS YAPGPAGLPGVPGKEGPPGFPGPPGPPGPPGKEGPPGVAGQKGSVGDVGIPGPKGSKGDLGPIGMPGKSG LAGSPGPVGPPGPPGPPGPPGPGFAAGFDDMEGSGIPLWTTARSSDGLQGPPGSPGLKGDPGVAGLPGAK GEVGADGAQGIPGPPGREGAAGSPGPKGEKGMPGEKGNPGKDGVGRPGLPGPPGPPGPVIYVSSEDKAIV STPGPEGKPGYAGFPGPAGPKGDLGSKGEQGLPGPKGEKGEPGTIFSPDGRALGHPQKGAKGEPGFRGPP GPYGRPGHKGEIGFPGRPGRPGTNGLKGEKGEPGDASLGFSMRGLPGPPGPPGPPGPPGMPIYDSNAFVE SGRPGLPGQQGVQGPSGPKGDKGEVGPPGPPGQFPIDLFHLEAEMKGDKGDRGDAGQKGERGEPGAPGGG FFSSSVPGPPGPPGYPGIPGPKGESIRGPPGPPGPQGPPGIGYEGRQGPPGPPGPPGPPSFPGPHRQTVS VPGPPGPPGPPGPPGAMGASAGQVRIWATYQTMLDKIREVPEGWLIFVAEREELYVRVRNGFRKVLLEAR TALPRGTGNEVAALQPPLVQLHEGSPYTRREYSYSTARPWRADDILANPPRLPDRQPYPGVPHHHSSYVH LPPARPTLSLAHTHQDFQPVLHLVALNTPLSGGMRGIRGADFQCFQQARAVGLSGTFRAFLSSRLQDLYS IVRRADRGSVPIVNLKDEVLSPSWDSLFSGSQGQLQPGARIFSFDGRDVLRHPAWPQKSVWHGSDPSGRR LMESYCETWRTETTGATGQASSLLSGRLLEQKAASCHNSYIVLCIENSFMTSFSK SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_009929 |
ORF Size | 3945 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_009929.3, NP_034059.2 |
RefSeq Size | 5058 bp |
RefSeq ORF | 3948 bp |
Locus ID | 12822 |
UniProt ID | P39061 |
MW | 134.7 kDa |
Gene Summary | Probably plays a major role in determining the retinal structure as well as in the closure of the neural tube.[UniProtKB/Swiss-Prot Function] |
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FAQs |
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