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Pnpla6 (BC054789) Mouse Tagged ORF Clone
CAT＃: MR211925
- TrueORF®
Pnpla6 (Myc-DDK-tagged) - Mouse patatin-like phospholipase domain containing 6 (cDNA clone MGC:65634 IMAGE:6831507)
ORF Plasmid: tGFP
"BC054789" in other vectors (4)
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CNY 13,210.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AI661849; MSws; Nte |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211925 representing BC054789
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGCCCCACTGCAAACCGGAATGGTGCTGGGCGTGATGATCGGGGCCGGAGTGGCGGTGCTGGTCA CCGCCGTGCTCATCCTCTTGGTGGTGCGAAGGCTGCGGGTGCAGAAAACTCCAGCCCCGGAGGGTCCCCG GTATCGGTTCCGGAAGCGGGACAAAGTGCTTTTCTATGGCCGGAAGATTATGCGGAAGGTGTCACAGTCC ACTTCTTCCCTGGTGGACACATCAGTCTCCACCACTTCCCGGCCCCGCATGAAGAAGAAACTGAAGATGC TTAACATTGCCAAGAAGATCTTACGTATCCAAAAGGAGACTCCGACCCTACAACGGAAGGAGCCCCCACC TTCAGTGCTGGAGGCTGACCTCACAGAGGGAGACCTGGCCAATTCCCACCTACCCTCTGAGGTACTCTAC ATGCTTAAGAATGTTCGGGTGCTAGGTCACTTTGAGAAGCCGCTCTTCCTGGAGCTCTGCCGGCACATGG TCTTCCAGCGTCTCGGGCAGGGGGACTATGTTTTTCGGCCCGGTCAACCAGATGCCAGCATCTATGTGGT TCAAGATGGGCTGCTGGAGCTCTGCCTACCAGGGCCTGATGGGAAGGAGTGTGTGGTGAAGGAGGTGGTT CCTGGAGACAGCGTCAACAGCCTTCTGAGCATCCTTGATGTCATCACGGGTCACCAGCATCCCCAGCGGA CTGTGTCAGCCAGGGCTGCACGGGACTCCACAGTGCTGAGACTCCCAGTAGAAGCCTTCTCTGCTGTCTT CACCAAGTACCCTGAGAGTTTGGTGCGAGTGGTACAGATCATCATGGTAAGGCTGCAGAGAGTCACCTTC CTGGCGCTTCACAATTACCTGGGTCTGACCAATGAACTCTTCAGTCATGAGATCCAGCCCCTACGCCTTT TCCCCAGCCCCGGCCTCCCGACCCGAACCAGCCCTGTCCGTGGCTCTAAACGGGTAGTCAGCACCTCAGG TACTGAGGACACCTCAAAGGAGACCTCTGGCCGGCCCCTGGACTCCATTGGAGCTCCTCTGCCTGGACCT GCAGGGGATCCCGTGAAGCCCACATCCTTAGAAGCACCCCCAGCCCCGTTACTGAGCCGCTGCATCTCCA TGCCAGTAGACATCTCAGGCTTGCAAGGTGGCCCTCGTTCTGATTTTGACATGGCCTATGAACGTGGACG GATATCTGTTTCTCTTCAAGAAGAGGCATCTGGGGGACCTCAGACAGCATCCCCTAGGGAGCTCCGGGAG CAGCCAGCGGGTGCCTGTGAATATAGCTACTGTGAGGATGAGTCAGCCACAGGCGGATGTCCCTTTGGGC CCTACCAGGGCCGCCAGACAAGCAGCATCTTTGAGGCTGCAAAGAGAGAGCTAGCCAAGCTGATGCGGAT TGAGGACCCCTCTCTACTGAACAGCAGAGTCTTGCTACATCATGCCAAAGCCGGCACCATCATAGCCCGC CAAGGGGACCAGGATGTGAGCCTGCACTTTGTGCTCTGGGGCTGCCTGCACGTGTACCAGCGCATGATTG ACAAGGCTGAGGAAGTGTGCCTATTTGTGGCACAGCCAGGGGAGCTGGTGGGGCAACTGGCGGTGCTCAC TGGGGAACCTCTCATCTTCACACTACGTGCCCAGAGAGACTGCACCTTCCTGCGGATCTCCAAATCCCAC TTCTATGAGATCATGCGTGCACAACCCAGTGTGGTACTGAGTGCAGCTCACACGGTGGCTGCTAGAATGT CCCCCTTCGTGCGCCAGATGGACTTTGCCATTGACTGGACAGCTGTGGAGGCTGGTCGTGCGCTCTACAG GCAGGGAGACCGCTCCGACTGCACCTACATTGTACTCAATGGACGTCTCCGTAGTGTCATCCAACGAGGC AGTGGCAAGAAGGAGCTAGTTGGGGAGTATGGTCGAGGGGATCTCATTGGCGTGGTGGAGGCACTGACCC GGCAGCCACGAGCCACCACTGTACACGCAGTGCGAGACACTGAGCTGGCCAAGCTTCCCGAGGGCACCTT AGGCCACATCAAACGTCGGTACCCACAGGTTGTGACCCGCCTTATTCATCTGCTAAGCCAGAAAATTCTA GGCAATCTGCAGCAGTTGCAAGGACCCTTTCCAGGCTCCGGGCTCAGTGTTCCGCAGCACTCGGAACTGA CCAACCCAGCCAGCAATCTGTCTACTGTAGCTATCCTCCCTGTGTGTGCTGAGGTGCCCATGATGGCCTT TACTCTGGAACTGCAGCATGCTCTGCAAGCTATTGGTCCCACGCTCCTCCTTAACAGTGACGTCATCCGG GCACTCCTGGGGGCTTCAGCACTGGATAGCATTCAAGAATTCCGGCTGTCAGGGTGGTTGGCCCAGCAGG AAGATGCCCATCGCATTGTGCTCTACCAAACTGACACATCCCTGACGCCTTGGACCGTCCGTTGTCTGCG CCAGGCCGACTGCATCCTCATTGTGGGCCTGGGGGACCAGGAACCCACTGTTGGCCAGCTGGAGCAAATG TTAGAGAACACTGCTGTCCGTGCCTTGAAGCAACTGGTTTTGCTGCACCGGGAGGAAGGCCCTGGTCCCA CGCGCACTGTAGAGTGGCTCAACATGCGCAGCTGGTGCTCAGGGCACCTGCACCTGCGCTGTCCTCGCCG CCTCTTCTCACGGCGCAGCCCAGCGAAACTTCATGAGCTGTATGAGAAGGTTTTCTCCAGGCGTGCAGAC CGTCACAGCGACTTCTCCCGCTTGGCACGAGTACTCACAGGAAACACTATTGCTCTGGTGCTGGGTGGGG GCGGAGCCAGAGGCTGCTCGCATATTGGGGTGCTGAAGGCATTAGAGGAGGCAGGAGTCCCAGTCGACCT TGTGGGAGGCACATCCATAGGTTCCTTCATTGGGGCCTTGTATGCCGAGGAACGCAGCGCCAGCCGAACT AAACAACGAGCCCGGGAGTGGGCCAAGAGCATGACTTCTGTACTGGAGCCTGTATTGGACCTCACATATC CTGTCACCTCCATGTTTACTGGCTCAGCCTTTAACAGAAGTATCCACCGTGTCTTCCAGGATAAGCAGAT TGAGGACCTGTGGCTGCCTTACTTCAATGTGACCACAGACATCACTGCCTCTGCCATGCGCGTCCACAAA GATGGCTCCCTGTGGCGGTATGTACGTGCCAGCATGACGCTCTCGGGCTACCTACCCCCGCTGTGCGACC CAAAGGATGGGCACCTGCTCATGGATGGTGGCTACATCAACAACCTGCCAGCGGATATTGCCCGAAGCAT GGGAGCCAAAACGGTCATTGCCATCGACGTTGGAAGCCAGGATGAGACAGATCTCAGCACCTATGGGGAC AGCCTGTCTGGCTGGTGGTTGCTGTGGAAAAGGCTAAACCCCTGGGCAGACAAGGTAAAGGTTCCAGACA TGGCTGAGATCCAGTCCCGCTTGGCGTACGTGTCCTGTGTGCGGCAGCTAGAAGTCGTCAAGTCCAGCTC CTATTGCGAGTACCTTCGTCCATCCATTGACTGCTTCAAGACCATGGACTTTGGAAAGTTTGACCAGATC TATGATGTGGGTTACCAGTATGGGAAGGCTGTCTTTGGAGGCTGGACCCGTGGTGAAGTCATTGAGAAAA TGCTCACAGACCGGAGATCTACAGACCTTAATGAGAGTCGCCGTGCAGATATACTTGCCTTCCCAAGTTC TGGGTTCACTGACTTGGCTGAGATTGTGTCCCGGATCGAGCCACCAACAAGCTACGTCTCTGATGGCTGT GCTGATGGGGAGGAGTCGGATTGCTTGACAGAGTATGAGGAAGATGCAGGACCAGACTGCTCAAGAGATG AAGGGGGCTCCCCTGAGGGTGCCAGCCCTAGCACTGCCTCAGAGGTGGAAGAAGAGAAGTCCACACTCCG GCAACGACGCTTTCTGCCTCAGGAGACTCCCAGCTCAGTCGCAGATGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211925 representing BC054789
Red=Cloning site Green=Tags(s) MEAPLQTGMVLGVMIGAGVAVLVTAVLILLVVRRLRVQKTPAPEGPRYRFRKRDKVLFYGRKIMRKVSQS TSSLVDTSVSTTSRPRMKKKLKMLNIAKKILRIQKETPTLQRKEPPPSVLEADLTEGDLANSHLPSEVLY MLKNVRVLGHFEKPLFLELCRHMVFQRLGQGDYVFRPGQPDASIYVVQDGLLELCLPGPDGKECVVKEVV PGDSVNSLLSILDVITGHQHPQRTVSARAARDSTVLRLPVEAFSAVFTKYPESLVRVVQIIMVRLQRVTF LALHNYLGLTNELFSHEIQPLRLFPSPGLPTRTSPVRGSKRVVSTSGTEDTSKETSGRPLDSIGAPLPGP AGDPVKPTSLEAPPAPLLSRCISMPVDISGLQGGPRSDFDMAYERGRISVSLQEEASGGPQTASPRELRE QPAGACEYSYCEDESATGGCPFGPYQGRQTSSIFEAAKRELAKLMRIEDPSLLNSRVLLHHAKAGTIIAR QGDQDVSLHFVLWGCLHVYQRMIDKAEEVCLFVAQPGELVGQLAVLTGEPLIFTLRAQRDCTFLRISKSH FYEIMRAQPSVVLSAAHTVAARMSPFVRQMDFAIDWTAVEAGRALYRQGDRSDCTYIVLNGRLRSVIQRG SGKKELVGEYGRGDLIGVVEALTRQPRATTVHAVRDTELAKLPEGTLGHIKRRYPQVVTRLIHLLSQKIL GNLQQLQGPFPGSGLSVPQHSELTNPASNLSTVAILPVCAEVPMMAFTLELQHALQAIGPTLLLNSDVIR ALLGASALDSIQEFRLSGWLAQQEDAHRIVLYQTDTSLTPWTVRCLRQADCILIVGLGDQEPTVGQLEQM LENTAVRALKQLVLLHREEGPGPTRTVEWLNMRSWCSGHLHLRCPRRLFSRRSPAKLHELYEKVFSRRAD RHSDFSRLARVLTGNTIALVLGGGGARGCSHIGVLKALEEAGVPVDLVGGTSIGSFIGALYAEERSASRT KQRAREWAKSMTSVLEPVLDLTYPVTSMFTGSAFNRSIHRVFQDKQIEDLWLPYFNVTTDITASAMRVHK DGSLWRYVRASMTLSGYLPPLCDPKDGHLLMDGGYINNLPADIARSMGAKTVIAIDVGSQDETDLSTYGD SLSGWWLLWKRLNPWADKVKVPDMAEIQSRLAYVSCVRQLEVVKSSSYCEYLRPSIDCFKTMDFGKFDQI YDVGYQYGKAVFGGWTRGEVIEKMLTDRRSTDLNESRRADILAFPSSGFTDLAEIVSRIEPPTSYVSDGC ADGEESDCLTEYEEDAGPDCSRDEGGSPEGASPSTASEVEEEKSTLRQRRFLPQETPSSVADA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC054789 |
| ORF Size | 3969 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC054789.1 |
| RefSeq Size | 4461 bp |
| RefSeq ORF | 3971 bp |
| Locus ID | 50767 |
| MW | 163.6 kDa |
| Gene Summary | Phospholipase B that deacylates intracellular phosphatidylcholine (PtdCho), generating glycerophosphocholine (GroPtdCho). This deacylation occurs at both sn-2 and sn-1 positions of PtdCho. Its specific chemical modification by certain organophosphorus (OP) compounds leads to distal axonopathy.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223084 | Pnpla6 (untagged) - Mouse patatin-like phospholipase domain containing 6 (cDNA clone MGC:65634 IMAGE:6831507), (10ug) |
CNY 12,920.00 |
|
| MG211925 | Pnpla6 (tGFP-tagged) - Mouse patatin-like phospholipase domain containing 6 (cDNA clone MGC:65634 IMAGE:6831507) |
CNY 14,540.00 |
|
| MR211925L3 | Lenti ORF clone of Pnpla6 (Myc-DDK-tagged) - Mouse patatin-like phospholipase domain containing 6 (cDNA clone MGC:65634 IMAGE:6831507) |
CNY 15,110.00 |
|
| MR211925L4 | Lenti ORF clone of Pnpla6 (mGFP-tagged) - Mouse patatin-like phospholipase domain containing 6 (cDNA clone MGC:65634 IMAGE:6831507) |
CNY 15,110.00 |
